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Article

Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents

by
Juriyati Jalil
1,*,
Carla W. Sabandar
1,
Norizan Ahmat
2,
Jamia A. Jamal
1,
Ibrahim Jantan
1,
Nor-Ashila Aladdin
1,
Kartiniwati Muhammad
1,
Fhataheya Buang
1,
Hazni Falina Mohamad
1 and
Idin Sahidin
3
1
Drug Herbal Research Centre, Faculty of Pharmacy, Universiti Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, Kuala Lumpur 50300, Malaysia
2
Faculty of Applied Sciences, Universiti Teknologi Mara, Shah Alam 40450, Malaysia
3
Laboratory of Natural Products Chemistry, Faculty of Pharmacy, Halu Oleo University, Kendari 93232, Indonesia
*
Author to whom correspondence should be addressed.
Molecules 2015, 20(2), 3206-3220; https://doi.org/10.3390/molecules20023206
Submission received: 13 November 2014 / Accepted: 30 January 2015 / Published: 16 February 2015
(This article belongs to the Section Natural Products Chemistry)
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Abstract

The crude methanol extracts and fractions of the root and stem barks of Dillenia serrata Thunb. showed 64% to 73% inhibition on the production of prostaglandin E2 (PGE2) in lipopolysaccharide-induced human whole blood using a radioimmunoassay technique. Three triterpenoids isolated from the root bark of the plant, koetjapic (1), 3-oxoolean-12-en-30-oic (2), and betulinic (3) acids, exhibited significant concentration-dependent inhibitory effects on PGE2 production with IC50 values of 1.05, 1.54, and 2.59 μM, respectively, as compared with the positive control, indomethacin (IC50 = 0.45 μM). Quantification of compounds 1 and 3 in the methanol extracts and fractions were carried out by using a validated reversed-phase high performance liquid chromatography (RP-HPLC) method. The ethyl acetate fraction of the stem bark showed the highest content of both compound 1 (15.1%) and compound 3 (52.8%). The strong inhibition of the extracts and fractions on cyclooxygenase-2 (COX-2) enzymatic activity was due to the presence of their major constituents, especially koetjapic and betulinic acids.
Keywords: Dilleniaceae; Dillenia serrata; triterpenoids; prostaglandin E2; HPLC Dilleniaceae; Dillenia serrata; triterpenoids; prostaglandin E2; HPLC

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MDPI and ACS Style

Jalil, J.; Sabandar, C.W.; Ahmat, N.; Jamal, J.A.; Jantan, I.; Aladdin, N.-A.; Muhammad, K.; Buang, F.; Mohamad, H.F.; Sahidin, I. Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents. Molecules 2015, 20, 3206-3220. https://doi.org/10.3390/molecules20023206

AMA Style

Jalil J, Sabandar CW, Ahmat N, Jamal JA, Jantan I, Aladdin N-A, Muhammad K, Buang F, Mohamad HF, Sahidin I. Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents. Molecules. 2015; 20(2):3206-3220. https://doi.org/10.3390/molecules20023206

Chicago/Turabian Style

Jalil, Juriyati, Carla W. Sabandar, Norizan Ahmat, Jamia A. Jamal, Ibrahim Jantan, Nor-Ashila Aladdin, Kartiniwati Muhammad, Fhataheya Buang, Hazni Falina Mohamad, and Idin Sahidin. 2015. "Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents" Molecules 20, no. 2: 3206-3220. https://doi.org/10.3390/molecules20023206

APA Style

Jalil, J., Sabandar, C. W., Ahmat, N., Jamal, J. A., Jantan, I., Aladdin, N.-A., Muhammad, K., Buang, F., Mohamad, H. F., & Sahidin, I. (2015). Inhibitory Effect of Triterpenoids from Dillenia serrata (Dilleniaceae) on Prostaglandin E2 Production and Quantitative HPLC Analysis of Its Koetjapic Acid and Betulinic Acid Contents. Molecules, 20(2), 3206-3220. https://doi.org/10.3390/molecules20023206

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