Anti-Inflammatory Activity of Heterocarpin from the Salt Marsh Plant Corydalis heterocarpa in LPS-Induced RAW 264.7 Macrophage Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Structure Determination of Compounds 1–5
2.2. Cell Viability
2.3. Effect of Compounds 1–5 on LPS-induced NO Production and iNOS Expression
2.4. Effect of Compound 1 on LPS-Induced PGE2 Production and COX-2 Expression
2.5. Effects of Compound 1 on Pro-Inflammatory Cytokine Production
2.6. Discussion
3. Experimental Section
3.1. General Procedures
3.2. Reagents
3.3. Plant Material
3.4. Extraction and Isolation
3.5. Cell Culture
3.6. Measurement of Cell Viability
3.7. Measurement of Nitrite and PGE2
3.8. Enzyme-Linked Immunosorbent Assay (ELISA)
3.9. Western Blot Analysis
3.10. Statistical Analysis
Acknowledgement
Author Contributions
Conflicts of Interest
References
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Kim, Y.A.; Kong, C.-S.; Park, H.H.; Lee, E.; Jang, M.-S.; Nam, K.-H.; Seo, Y. Anti-Inflammatory Activity of Heterocarpin from the Salt Marsh Plant Corydalis heterocarpa in LPS-Induced RAW 264.7 Macrophage Cells. Molecules 2015, 20, 14474-14486. https://doi.org/10.3390/molecules200814474
Kim YA, Kong C-S, Park HH, Lee E, Jang M-S, Nam K-H, Seo Y. Anti-Inflammatory Activity of Heterocarpin from the Salt Marsh Plant Corydalis heterocarpa in LPS-Induced RAW 264.7 Macrophage Cells. Molecules. 2015; 20(8):14474-14486. https://doi.org/10.3390/molecules200814474
Chicago/Turabian StyleKim, You Ah, Chang-Suk Kong, Hyo Hyun Park, Eunkyung Lee, Mi-Soon Jang, Ki-Ho Nam, and Youngwan Seo. 2015. "Anti-Inflammatory Activity of Heterocarpin from the Salt Marsh Plant Corydalis heterocarpa in LPS-Induced RAW 264.7 Macrophage Cells" Molecules 20, no. 8: 14474-14486. https://doi.org/10.3390/molecules200814474