The Application of Ultra-High-Performance Liquid Chromatography Coupled with a LTQ-Orbitrap Mass Technique to Reveal the Dynamic Accumulation of Secondary Metabolites in Licorice under ABA Stress
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material and Reagents
2.2. Sample Processing
2.3. Chromatographic Conditions
2.4. MS Conditions
2.5. Statistical Analysis
3. Result and Discussion
3.1. Flavonoids and Triterpenes in the Targeted Metabolomics Study
3.2. Comparison of Content of Licorice Samples in Two Groups
3.3. Principal Component Analysis
3.4. Partial Least Squares Discriminant Analysis
3.5. Analysis of Metabolic Pathways of Two Compounds
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds in this paper are available from the authors. |
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Li, D.; Xu, G.; Ren, G.; Sun, Y.; Huang, Y.; Liu, C. The Application of Ultra-High-Performance Liquid Chromatography Coupled with a LTQ-Orbitrap Mass Technique to Reveal the Dynamic Accumulation of Secondary Metabolites in Licorice under ABA Stress. Molecules 2017, 22, 1742. https://doi.org/10.3390/molecules22101742
Li D, Xu G, Ren G, Sun Y, Huang Y, Liu C. The Application of Ultra-High-Performance Liquid Chromatography Coupled with a LTQ-Orbitrap Mass Technique to Reveal the Dynamic Accumulation of Secondary Metabolites in Licorice under ABA Stress. Molecules. 2017; 22(10):1742. https://doi.org/10.3390/molecules22101742
Chicago/Turabian StyleLi, Da, Guojie Xu, Guangxi Ren, Yufeng Sun, Ying Huang, and Chunsheng Liu. 2017. "The Application of Ultra-High-Performance Liquid Chromatography Coupled with a LTQ-Orbitrap Mass Technique to Reveal the Dynamic Accumulation of Secondary Metabolites in Licorice under ABA Stress" Molecules 22, no. 10: 1742. https://doi.org/10.3390/molecules22101742