Studying the Structural Significance of Galectin Design by Playing a Modular Puzzle: Homodimer Generation from Human Tandem-Repeat-Type (Heterodimeric) Galectin-8 by Domain Shuffling
Abstract
:1. Introduction
2. Results
2.1. Protein Production and Characterization
2.1.1. Engineering and Yield
2.1.2. Structural Characterization
2.1.3. Glycan Specificity Profile
2.1.4. Cell Binding and Aggregation
3. Discussion
4. Materials and Methods
4.1. cDNA Engineering and Protein Production
4.2. Analytical Procedures
4.3. Glycan Array
4.4. Solid-Phase and Cell Assays
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Not available. |
Structure | Median | MAD # |
---|---|---|
Neu5Acα2,3Galβ1,3(6-O-Su)GlcNAc | 59,796 | 1931 |
Neu5Acα2,3Galβ1,4Glc-Gly-Phe * | 43,636 | 4717 |
Neu5Acα2,8Neu5Acα2,3Galβl,4Glc | 31,204 | 1141 |
Neu5Aα2,3Galβ1,3GlcNAcβ1,3Galβ1,4Glc | 18,278 | 6705 |
Neu5Acα2,3Galβ1,3GlcNAc | 17,215 | 1619 |
Neu5Acα2,3Galβ1,4Glc | 8888 | 294 |
3-O-Su-Galβ1,4(6-O-Su)Glc | 5279 | 146 |
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Ludwig, A.-K.; Michalak, M.; Shilova, N.; André, S.; Kaltner, H.; Bovin, N.V.; Kopitz, J.; Gabius, H.-J. Studying the Structural Significance of Galectin Design by Playing a Modular Puzzle: Homodimer Generation from Human Tandem-Repeat-Type (Heterodimeric) Galectin-8 by Domain Shuffling. Molecules 2017, 22, 1572. https://doi.org/10.3390/molecules22091572
Ludwig A-K, Michalak M, Shilova N, André S, Kaltner H, Bovin NV, Kopitz J, Gabius H-J. Studying the Structural Significance of Galectin Design by Playing a Modular Puzzle: Homodimer Generation from Human Tandem-Repeat-Type (Heterodimeric) Galectin-8 by Domain Shuffling. Molecules. 2017; 22(9):1572. https://doi.org/10.3390/molecules22091572
Chicago/Turabian StyleLudwig, Anna-Kristin, Malwina Michalak, Nadya Shilova, Sabine André, Herbert Kaltner, Nicolai V. Bovin, Jürgen Kopitz, and Hans-Joachim Gabius. 2017. "Studying the Structural Significance of Galectin Design by Playing a Modular Puzzle: Homodimer Generation from Human Tandem-Repeat-Type (Heterodimeric) Galectin-8 by Domain Shuffling" Molecules 22, no. 9: 1572. https://doi.org/10.3390/molecules22091572