Chemical Constituents of Vigna luteola and Their Anti-inflammatory Bioactivity
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purification and Characterization
2.2. Structural Elucidations of 1–5
2.3. Anti-Inflammatory Activity
3. Experimental Section
3.1. General
3.2. Plant Materials
3.3. Extraction and Isolation
3.3.1. Viglutin (1)
3.3.2. Viglutoside (2)
3.3.3. Viglutanone (3)
3.3.4. Sodium Phaseate (4)
3.3.5. Sodium p-Coumarate (5)
3.4. Anti-Inflammatory Bioactivity Examination
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of all the isolated compounds are available from the authors. |
Samples | Inh % a | |
---|---|---|
Superoxide Anion Generation | Elastase Release | |
methanol extract | 51.8 ± 6.8 *** | 108.4 ± 6.9 *** |
chloroform fraction | 91.2 ± 3.8 *** | 118.0 ± 5.0 *** |
water fraction | 18.3 ± 6.1 * | 34.6 ± 3.6 *** |
Compound | Superoxide Anion Generation | Elastase Release | ||
---|---|---|---|---|
IC50 (μM) a | Inh % b | IC50 (μM) | Inh % | |
26 | 6.1 ± 0.3 | 69.9 ± 4.4 *** | – c | 11.8 ± 2.1 ** |
54 | 4.5 ± 0.3 | 93.6 ± 3.3 *** | – | 23.7 ± 1.1 *** |
55 | 4.1 ± 0.2 | 99.0 ± 1.9 *** | 3.8 ± 0.1 | 89.4 ± 4.5 *** |
57 | 5.0 ± 0.4 | 88.4 ± 5.3 *** | 4.7 ± 0.4 | 89.9 ± 2.2 *** |
63 | 9.3 ± 0.3 | 52.5 ± 1.2 *** | 4.9 ± 0.2 | 75.2 ± 3.2 *** |
65 | 1.9 ± 0.2 | 89.3 ± 2.9 *** | 6.4 ± 0.7 | 61.3 ± 4.7 *** |
66 | – | 27.4 ± 7.5 * | 7.7 ± 0.5 | 60.4 ± 2.3 *** |
67 | 3.2 ± 0.1 | 100.0 ± 1.3 *** | 4.1 ± 0.7 | 99.6 ± 7.6 *** |
70 | 5.6 ± 0.9 | 85.2 ± 9.7 *** | – | 46.5 ± 2.2 *** |
LY294002d | 1.0 ± 0.2 | 3.1 ± 0.7 |
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Lam, S.-H.; Li, Y.-C.; Kuo, P.-C.; Hwang, T.-L.; Yang, M.-L.; Wang, C.-C.; Tzen, J.T.C. Chemical Constituents of Vigna luteola and Their Anti-inflammatory Bioactivity. Molecules 2019, 24, 1371. https://doi.org/10.3390/molecules24071371
Lam S-H, Li Y-C, Kuo P-C, Hwang T-L, Yang M-L, Wang C-C, Tzen JTC. Chemical Constituents of Vigna luteola and Their Anti-inflammatory Bioactivity. Molecules. 2019; 24(7):1371. https://doi.org/10.3390/molecules24071371
Chicago/Turabian StyleLam, Sio-Hong, Yue-Chiun Li, Ping-Chung Kuo, Tsong-Long Hwang, Mei-Lin Yang, Chien-Chiao Wang, and Jason T. C. Tzen. 2019. "Chemical Constituents of Vigna luteola and Their Anti-inflammatory Bioactivity" Molecules 24, no. 7: 1371. https://doi.org/10.3390/molecules24071371