Antiadipogenic Effects of Mixtures of Cornus officinalis and Ribes fasciculatum Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice
Abstract
:1. Introduction
2. Results
2.1. Effects of CO and RF on Adipogenesis in Vitro
2.2. Effects of CO+RF Extract Mixture on Adipogenesis in 3T3-L1 Cells
2.3. Anti-Obesity Effects of CO and RF in HFD-Induced Murine Obesity Model
2.4. HPLC Profile of CO and RF Extracts
3. Discussion
4. Materials and Methods
4.1. Preparation of CO and RF Extracts
4.2. HPLC-DAD Analysis of CO and RF Extracts
4.3. Cell Culture and Adipocyte Differentiation
4.4. Cell Viability
4.5. Quantitative Reverse-Transcription Polymerase Chain Reaction
4.6. Oil Red O Staining of Adipocytes
4.7. In Vivo Experiments in an Obesity Murine Model
4.8. Preparation of Tissue Samples and Histology
4.9. Statistical Analysis
5. Conclusion
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
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Park, E.; Lee, C.G.; Jeong, H.; Yeo, S.; Kim, J.A.; Jeong, S.-Y. Antiadipogenic Effects of Mixtures of Cornus officinalis and Ribes fasciculatum Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice. Molecules 2020, 25, 2350. https://doi.org/10.3390/molecules25102350
Park E, Lee CG, Jeong H, Yeo S, Kim JA, Jeong S-Y. Antiadipogenic Effects of Mixtures of Cornus officinalis and Ribes fasciculatum Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice. Molecules. 2020; 25(10):2350. https://doi.org/10.3390/molecules25102350
Chicago/Turabian StylePark, Eunkuk, Chang Gun Lee, Hyesoo Jeong, Subin Yeo, Ji Ae Kim, and Seon-Yong Jeong. 2020. "Antiadipogenic Effects of Mixtures of Cornus officinalis and Ribes fasciculatum Extracts on 3T3-L1 Preadipocytes and High-Fat Diet-Induced Mice" Molecules 25, no. 10: 2350. https://doi.org/10.3390/molecules25102350