Antimicrobial Potential of Essential Oils from Cerrado Plants against Multidrug−Resistant Foodborne Microorganisms
Abstract
:1. Introduction
2. Results
2.1. Antimicrobial Screening of Cerrado Plants Essential Oils
2.2. Chemical Profile of the Bioactive Essential Oils
3. Discussion
4. Materials and Methods
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the essential oils are available from the authors. |
MIC (µg·mL−1) | |||||||
---|---|---|---|---|---|---|---|
Plant species/Essential Oil | E. coli NEWP0022 | E. coli NEWP 0018 | S. aureus NEWP 0023 | P. aeruginosa NEWP0027 | Staphylococcus sp. 841 | Staphylococcus sp. 873 | Salmonella Typhi 905 |
Chromolaena squalida | >1000 | >1000 | 125 | 500 | 7.80 | 250 | >1000 |
Campomanesia sessiliflora | 500 | >1000 | 250 | 500 | 31.25 | 250 | 500 |
Myrsine guianensis | 500 | >1000 | 500 | 500 | 31.25 | 500 | >1000 |
Matayba guianensis | 500 | 500 | 500 | 500 | 125 | 500 | >1000 |
Siparuna guianensis | >1000 | >1000 | 500 | 500 | 500 | 500 | >1000 |
Ocotea minarum | 500 | >1000 | 250 | 500 | 250 | 250 | >1000 |
Endlicheria paniculata | 500 | >1000 | 500 | 500 | 500 | 250 | >1000 |
Gentamincin | ≤0.5 | ≤0.5 | ≤0.5 | 3.5 | ≤0.5 | 3.5 | ≤0.5 |
Compounds | Molecular Formula | RI + Exp | RI ++ Ref. | Chromolaena squalida | Campomanesia sessiliflora | Myrsine guianensis | Matayba guianensis |
---|---|---|---|---|---|---|---|
Peak Area (%) | |||||||
α-Thujene 1 | C10H16 | 922 | 924 | 0.27 | 0.27 | 0.01 | - |
α-Pinene 2 | C10H16 | 930 | 932 | 1.00 | 38.65 | - | - |
Camphene 3 | C10H16 | 944 | 946 | - | 0.03 | - | - |
Sabinene 4 | C10H16 | 968 | 969 | 0.06 | - | - | - |
β-Pinene 5 | C10H16 | 972 | 974 | 1.38 | 0.69 | - | - |
Myrcene 6 | C10H16 | 986 | 988 | 1.35 | - | - | - |
α-Phellandrene 7 | C10H16 | 1002 | 1002 | 0.04 | 0.20 | - | - |
α-Terpinene 8 | C10H16 | 1013 | 1014 | 0.10 | - | - | - |
o-Cymene 9 | C10H14 | 1021 | 1022 | 0.14 | 0.10 | - | - |
Limonene 10 | C10H16 | 1024 | 1024 | 2.18 | 2.10 | - | - |
(Z)-β-Ocimene 11 | C10H16 | 1033 | 1032 | 0.14 | - | - | - |
(E)-β-Ocimene 12 | C10H16 | 1043 | 1044 | 4.77 | - | - | - |
γ-Terpinene 13 | C10H16 | 1054 | 1054 | 0.30 | 0.17 | - | - |
Terpinolene 14 | C10H16 | 1085 | 1086 | 0.76 | 0.07 | - | - |
Eucalyptol 15 | C10H18O | 1027 | 1026 | - | 2.74 | 0.02 | - |
Linalool 16 | C10H18O | 1097 | 1095 | 0.15 | 1.67 | 0.05 | - |
Terpinen-4-ol 17 | C10H18O | 1173 | 1174 | 0.36 | 0.04 | - | - |
α-Terpineol 18 | C10H18O | 1188 | 1186 | 0.19 | 0.37 | - | - |
δ-Elemene 19 | C15H24 | 1332 | 1335 | 0.91 | - | - | 2.73 |
α-Cubebene 20 | C15H24 | 1344 | 1348 | 0.36 | - | 0.44 | 0.14 |
Cyclosativene 21 | C15H24 | 1362 | 1369 | 0.22 | - | - | - |
α-Ylangene 22 | C15H24 | 1366 | 1373 | 0.37 | - | 0.59 | 0.24 |
Isoledene 23 | C15H24 | 1368 | 1374 | 0.19 | 0.32 | - | 0.47 |
α-Copaene 24 | C15H24 | 1371 | 1374 | 1.95 | 0.11 | 7.00 | 1.34 |
β-Bourbonene 25 | C15H24 | 1380 | 1387 | 0.32 | - | 0.30 | 0.21 |
β-Elemene 26 | C15H24 | 1387 | 1389 | 1.26 | 0.19 | 0.55 | 1.95 |
α-Gurjunene 27 | C15H24 | 1405 | 1409 | 0.31 | 1.08 | 0.06 | 0.21 |
Isocaryophyllene 28 | C15H24 | 1414 | 1408 | - | - | 21.81 | - |
β-Caryophyllene 29 | C15H24 | 1415 | 1417 | 12.54 | 13.35 | - | 15.45 |
Aromadendrene 30 | C15H24 | 1434 | 1439 | 0.32 | 12.52 | 7.82 | 1.28 |
α-Humulene 31 | C15H24 | 1448 | 1452 | 1.92 | 1.69 | 7.80 | 2.10 |
(E)-β-Farnesene 32 | C15H24 | 1452 | 1454 | - | - | 0.68 | - |
α-Patchoulene 33 | C15H24 | 1456 | 1454 | 1.26 | 3.57 | 0.77 | 0.81 |
10-β-Cadina-1(6),4-diene 34 | C15H24 | 1468 | 1475 | 1.38 | - | - | - |
α-Amorphene 35 | C15H24 | 1473 | 1483 | - | - | - | 1.37 |
Germacrene D 36 | C15H24 | 1478 | 1480 | 12.74 | - | 1.95 | 28.39 |
β-Selinene 37 | C15H24 | 1482 | 1489 | 0.65 | 0.47 | 3.51 | 0.33 |
γ-Muurolene 38 | C15H24 | 1482 | 1478 | 4.85 | 0.42 | 7.05 | - |
Valencene 39 | C15H24 | 1490 | 1496 | - | - | 11.52 | - |
Viridiflorene 40 | C15H24 | 1490 | 1496 | 14.32 | 7.55 | - | - |
Bicyclogermacrene 41 | C15H24 | 1493 | 1500 | - | - | 0.37 | 31.32 |
α-Muurolene 42 | C15H24 | 1496 | 1500 | 3.29 | - | 0.64 | 0.56 |
γ-Cadinene 43 | C15H24 | 1510 | 1513 | 4.06 | 0.77 | 4.45 | - |
δ-Cadinene 44 | C15H24 | 1519 | 1522 | 11.60 | - | 11.03 | 0.76 |
trans-Cadina-1,4-diene 45 | C15H24 | 1527 | 1533 | 0.73 | - | - | - |
α-Calacorene 46 | C15H24 | 1539 | 1544 | - | 0.10 | - | - |
Germacrene B 47 | C15H24 | 1552 | 1559 | 2.49 | - | 3.02 | 5.19 |
Elemol 48 | C15H26O | 1545 | 1548 | - | 0.14 | - | - |
Spathulenol 49 | C15H24O | 1574 | 1577 | - | 6.46 | 2.21 | 2.29 |
(E)-Nerolidol 50 | C15H26O | 1559 | 1561 | - | - | 4.71 | - |
Viridiflorol 51 | C15H26O | 1598 | 1592 | 8.74 | 1.44 | 0.89 | 1.83 |
5-epi-7-epi-α-Eudesmol 52 | C15H26O | 1598 | 1607 | - | - | - | 0.67 |
γ-Eudesmol 53 | C15H26O | 1628 | 1630 | - | 0.59 | - | - |
epi-α-Muurolol 54 | C15H26O | 1637 | 1640 | - | 0.62 | - | - |
δ-cadinol 55 | C15H26O | 1643 | 1644 | - | 0.12 | 0.48 | - |
β-Eudesmol 56 | C15H26O | 1649 | 1649 | - | 1.36 | 0.19 | - |
Identified compounds (%) | 99.97 | 99.97 | 99.92 | 99.64 | |||
Monoterpene Hydrocarbons (%) | 12.49 | 42.28 | 0.01 | - | |||
Oxygenated Monoterpenes (%) | 0.70 | 4.82 | 0.07 | - | |||
Sesquiterpene Hydrocarbons (%) | 78.04 | 42.14 | 91.36 | 94.85 | |||
Oxygenated Sesquiterpenes (%) | 8.74 | 10.73 | 8.48 | 4.79 |
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de Jesus, G.S.; Micheletti, A.C.; Padilha, R.G.; de Souza de Paula, J.; Alves, F.M.; Leal, C.R.B.; Garcez, F.R.; Garcez, W.S.; Yoshida, N.C. Antimicrobial Potential of Essential Oils from Cerrado Plants against Multidrug−Resistant Foodborne Microorganisms. Molecules 2020, 25, 3296. https://doi.org/10.3390/molecules25143296
de Jesus GS, Micheletti AC, Padilha RG, de Souza de Paula J, Alves FM, Leal CRB, Garcez FR, Garcez WS, Yoshida NC. Antimicrobial Potential of Essential Oils from Cerrado Plants against Multidrug−Resistant Foodborne Microorganisms. Molecules. 2020; 25(14):3296. https://doi.org/10.3390/molecules25143296
Chicago/Turabian Stylede Jesus, Genilson Silva, Ana Camila Micheletti, Rafael Gonçalves Padilha, Jessica de Souza de Paula, Flavio Macedo Alves, Cassia Rejane Brito Leal, Fernanda Rodrigues Garcez, Walmir Silva Garcez, and Nidia Cristiane Yoshida. 2020. "Antimicrobial Potential of Essential Oils from Cerrado Plants against Multidrug−Resistant Foodborne Microorganisms" Molecules 25, no. 14: 3296. https://doi.org/10.3390/molecules25143296