Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease
Abstract
:1. Introduction
2. Results and Discussion
2.1. MG132-Induced In Vitro Model for PD Using hiNPCs from PD Patient
2.2. Cytoprotective Effects of CTN on the In Vitro PD Model
2.3. Cell Death Attenuation by CTN
2.4. Recovery of Mitochondrial Functions by CTN
2.5. Induction of NRF2-Mediated Oxidative Stress Response by CTN
2.6. Expression of Antioxidative and Mitochondrial Biogenesis Molecules by CTN
3. Materials and Methods
3.1. Cell Culture
3.2. Cell Viability Assay
3.3. Flow Cytometry Analysis
3.4. Western Blot Analysis
3.5. Real-Time PCR for mRNA Quantification
3.6. Immunofluorescence
3.7. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compound are available from the authors. |
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Lee, J.-E.; Sim, H.; Yoo, H.M.; Lee, M.; Baek, A.; Jeon, Y.-J.; Seo, K.-S.; Son, M.-Y.; Yoon, J.S.; Kim, J. Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease. Molecules 2020, 25, 3602. https://doi.org/10.3390/molecules25163602
Lee J-E, Sim H, Yoo HM, Lee M, Baek A, Jeon Y-J, Seo K-S, Son M-Y, Yoon JS, Kim J. Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease. Molecules. 2020; 25(16):3602. https://doi.org/10.3390/molecules25163602
Chicago/Turabian StyleLee, Joo-Eun, Hyuna Sim, Hee Min Yoo, Minhyung Lee, Aruem Baek, Young-Joo Jeon, Kang-Sik Seo, Mi-Young Son, Joo Seog Yoon, and Janghwan Kim. 2020. "Neuroprotective Effects of Cryptotanshinone in a Direct Reprogramming Model of Parkinson’s Disease" Molecules 25, no. 16: 3602. https://doi.org/10.3390/molecules25163602