The Effect of GD1a Ganglioside-Expressing Bacterial Strains on Murine Norovirus Infectivity
Abstract
:1. Introduction
2. Results
2.1. The Western Blotting Result for GD1a Expression Confirmation
2.2. The Effect of GD1a-Expressing Bacterial Strains on the MNV Binding Profile
2.3. The Effect on MNV Infectivity
3. Discussion
4. Materials and Methods
4.1. Screening Out GD1a-Positive Bacterial Strains from Sewage Sample
4.2. Preparation of Media, Bacterial Suspension, and Virus Stock
4.3. ELISA for MNV–Bacterium Binding Assessment
4.4. Plaque Assay in the Presence of Bacterial Components
4.5. Viral Infectivity Assessment
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: The bacterial strains are available from the authors upon request. |
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Zhu, Y.; Kawai, H.; Hashiba, S.; Amarasiri, M.; Kitajima, M.; Okabe, S.; Sano, D. The Effect of GD1a Ganglioside-Expressing Bacterial Strains on Murine Norovirus Infectivity. Molecules 2020, 25, 4084. https://doi.org/10.3390/molecules25184084
Zhu Y, Kawai H, Hashiba S, Amarasiri M, Kitajima M, Okabe S, Sano D. The Effect of GD1a Ganglioside-Expressing Bacterial Strains on Murine Norovirus Infectivity. Molecules. 2020; 25(18):4084. https://doi.org/10.3390/molecules25184084
Chicago/Turabian StyleZhu, Yifan, Hiroki Kawai, Satoshi Hashiba, Mohan Amarasiri, Masaaki Kitajima, Satoshi Okabe, and Daisuke Sano. 2020. "The Effect of GD1a Ganglioside-Expressing Bacterial Strains on Murine Norovirus Infectivity" Molecules 25, no. 18: 4084. https://doi.org/10.3390/molecules25184084