Effect of Strain, Wood Substrate and Cold Treatment on the Yield and β-Glucan Content of Ganoderma lucidum Fruiting Bodies
Abstract
:1. Introduction
2. Results
2.1. Effect of Strain, Wood Substrate and Cold Treatment on Successful Fruiting
2.2. Effect of Strain, Wood Substrate and Cold Treatment on Yield
2.3. Effect of Strain and Wood Substrate on Glucan Contents
3. Discussion
4. Materials and Methods
4.1. Fungal Strains
4.2. Spawn Production
4.3. Substrate Preparation
4.4. Cold Treatment and Fruiting Body Yield
4.5. β-Glucan Content Analysis
4.6. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Variables | Estimate | SE | Wald χ2 | p-Value |
---|---|---|---|---|
Constant | −1.30 | 0.57 | 5.20 | 0.023 |
Strain (df = 5, ref. MU19) | 41.82 | <0.001 | ||
MUS192 | −1.81 | 0.65 | 7.74 | 0.005 |
MUS6 | −2.15 | 0.66 | 10.54 | 0.001 |
MUS75 | −4.82 | 0.82 | 34.52 | <0.001 |
MUS9 | −2.80 | 0.68 | 16.86 | <0.001 |
MUS12 | −3.65 | 0.72 | 25.89 | <0.001 |
Wood (df = 4, ref. P. abies) | 13.97 | 0.007 | ||
Betula spp. | 0.30 | 0.55 | 0.30 | 0.583 |
Larix sp. | −0.81 | 0.58 | 1.98 | 0.160 |
P. sylvestris | −0.15 | 0.56 | 0.08 | 0.781 |
P. tremula | 1.33 | 0.56 | 5.61 | 0.018 |
Treatment +5 °C (df = 1, ref. −20 °C) | 4.14 | 0.54 | 58.78 | <0.001 |
Source | Numerator df | Denominator df | F | p-Value |
---|---|---|---|---|
Intercept | 1 | 69 | 308.99 | <0.001 |
Strain | 5 | 69 | 3.40 | 0.008 |
Wood | 4 | 69 | 9.71 | <0.001 |
Treatment | 1 | 69 | 0.48 | 0.492 |
Strain * Wood | 14 | 69 | 1.12 | 0.361 |
Wood * Treatment | 1 | 69 | 0.15 | 0.697 |
Source | Num. Df | Den. Df | Total Glucan | α-Glucan | β-Glucan | |||
---|---|---|---|---|---|---|---|---|
F | p-Value | F | p-Value | F | p-Value | |||
Intercept | 1 | 61 | 23,708.09 | <0.001 | 204.55 | <0.001 | 19,374.09 | <0.001 |
Strain | 5 | 61 | 3.63 | 0.006 | 1.11 | 0.364 | 3.31 | 0.010 |
Wood | 4 | 61 | 17.28 | <0.001 | 1.37 | 0.256 | 15.60 | <0.001 |
Strain * Wood | 14 | 61 | 1.15 | 0.335 | 1.14 | 0.348 | 1.09 | 0.385 |
Strain | Region | Host Species | Isolation Date | ITS GenBank ID |
---|---|---|---|---|
MUS192 | Uusimaa | P. abies | 07/2016 | MT334582 |
MUS6 | Uusimaa | P. abies | 07/2016 | MT334583 |
MUS75 | Uusimaa | P. abies | 07/2016 | MT334584 |
MUS9 | Uusimaa | P. abies | 07/2016 | MT334585 |
MUS12 | Satakunta | P. abies | 10/2016 | MT334586 |
MUS19 | Uusimaa | B. pubescens | 03/2017 | MT334587 |
Sample Availability: Samples of the compounds are not available from the authors. |
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Cortina-Escribano, M.; Pihlava, J.-M.; Miina, J.; Veteli, P.; Linnakoski, R.; Vanhanen, H. Effect of Strain, Wood Substrate and Cold Treatment on the Yield and β-Glucan Content of Ganoderma lucidum Fruiting Bodies. Molecules 2020, 25, 4732. https://doi.org/10.3390/molecules25204732
Cortina-Escribano M, Pihlava J-M, Miina J, Veteli P, Linnakoski R, Vanhanen H. Effect of Strain, Wood Substrate and Cold Treatment on the Yield and β-Glucan Content of Ganoderma lucidum Fruiting Bodies. Molecules. 2020; 25(20):4732. https://doi.org/10.3390/molecules25204732
Chicago/Turabian StyleCortina-Escribano, Marta, Juha-Matti Pihlava, Jari Miina, Pyry Veteli, Riikka Linnakoski, and Henri Vanhanen. 2020. "Effect of Strain, Wood Substrate and Cold Treatment on the Yield and β-Glucan Content of Ganoderma lucidum Fruiting Bodies" Molecules 25, no. 20: 4732. https://doi.org/10.3390/molecules25204732