SiO2-PVA-Fe(acac)3 Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay
Abstract
:1. Introduction
2. Results and Discussion
2.1. Nitrogen Porosimetry
2.2. Thermal Analysis
2.3. Transmission Electron Microscopy
2.4. Small Angle Neutron Scattering (SANS)
2.5. Crystallite Size
2.6. Zeta Potential Measurements
2.7. Toxicological Evaluation of Magnetic Nanocomposite P-260
2.8. Evaluation of P-260 (NC) Nanocomposite by Studying the Effect on Human Keratinocytes Migration and Proliferation Potential
3. Materials and Methods
3.1. Materials and Synthesis Protocol
3.2. Characterization
3.3. In vitro Cytotoxicity Testing
3.4. Cell Culture
3.5. Cell Viability Assessment—MTT [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] Assay
3.6. Migratory and Proliferative Potential – aA Wound Healing Technique
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample Availability: The synthetized samples from this study are available from the authors. |
Samp. | SBET*1 [m2/g] | S L*2 [m2/g] | S t-plot*3 [m2/g] | dDFT*4 [nm] | dBJH*5 [nm] | TPV *6 [cm3/g] | Alpha-S*7 [cm3/g] | dfs FHH ads*8 | dfs FHH des*8 |
---|---|---|---|---|---|---|---|---|---|
P-120 | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A |
P-220 | 99 | 158 | 64 | 2.7 | 3.7 | 0.055 | 0.038 | 2.69/2.89 | 2.77/2.92 |
P-260 | 344 | 519 | 246 | 2.6 | 3.1 | 0.183 | 0.131 | 2.77/2.92 | 2.57/2.85 |
P-300 | 468 | 640 | 349 | 2.1 | 3.4 | 0.227 | 0.170 | 2.85/2.95 | 2.68/2.89 |
P-400 | 382 | 539 | 258 | 2.4 | 3.1 | 0.194 | 0.130 | 2.82/2.94 | 2.55/2.85 |
ΔT, [°C] | Tmax, [°C] | Weight Loss | Thermic Effect | ||
---|---|---|---|---|---|
ΔG/ΔT [%] | Cumulativ ΔG, [%] | ΔG/ΔT [mg] | |||
25–125 | 71 | 4.16 | 4.16 | 0.83 | endothermic |
125–215 | 193 | 8.27 | 12.43 | 1.65 | exothermic |
215–275 | 257 | 16.69 | 29.27 | 3.34 | exothermic |
275–800 | 361 | 22.31 | 51.44 | 4.46 | exothermic |
Sample Name | Calcination Temperature | Rg (nm) | p | Fractal Dimension |
---|---|---|---|---|
P-120 | 120 °C | - | 3.82 ± 0.03 | Dsurface = 2.18 |
P-220 | 220 °C | 10.01 ± 0.09 | 2.42 ± 0.01 | Dvolume = 2.42 |
P-260 | 260 °C | 7.54 ± 0.02 | 2.21 ± 0.01 | Dvolume = 2.21 |
P-300 | 300 °C | 7.79 ± 0.02 | 2.26 ± 0.01 | Dvolume = 2.26 |
P-400 | 400 °C | 9.59 ± 0.03 | 2.18 ± 0.01 | Dvolume =2.18 |
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Putz, A.-M.; Ianăși, C.; Dudás, Z.; Coricovac, D.; Watz, C.; Len, A.; Almásy, L.; Sacarescu, L.; Dehelean, C. SiO2-PVA-Fe(acac)3 Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay. Molecules 2020, 25, 653. https://doi.org/10.3390/molecules25030653
Putz A-M, Ianăși C, Dudás Z, Coricovac D, Watz C, Len A, Almásy L, Sacarescu L, Dehelean C. SiO2-PVA-Fe(acac)3 Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay. Molecules. 2020; 25(3):653. https://doi.org/10.3390/molecules25030653
Chicago/Turabian StylePutz, Ana-Maria, Cătălin Ianăși, Zoltán Dudás, Dorina Coricovac, Claudia (Farcas) Watz, Adél Len, László Almásy, Liviu Sacarescu, and Cristina Dehelean. 2020. "SiO2-PVA-Fe(acac)3 Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay" Molecules 25, no. 3: 653. https://doi.org/10.3390/molecules25030653
APA StylePutz, A. -M., Ianăși, C., Dudás, Z., Coricovac, D., Watz, C., Len, A., Almásy, L., Sacarescu, L., & Dehelean, C. (2020). SiO2-PVA-Fe(acac)3 Hybrid Based Superparamagnetic Nanocomposites for Nanomedicine: Morpho-textural Evaluation and In Vitro Cytotoxicity Assay. Molecules, 25(3), 653. https://doi.org/10.3390/molecules25030653