Evaluation of the Antinociceptive, Antiallodynic, Antihyperalgesic and Anti-Inflammatory Effect of Polyalthic Acid
Abstract
:1. Introduction
2. Results
2.1. Antinociceptive Effect of Polyalthic Acid
2.2. Possible Mechanisms of the Antinociceptive Effect of Polyalthic Acid
2.3. Antiallodynic Effect of Polyalthic Acid on Rats with an L5/L6 Spinal Nerve Ligation
2.4. Antihyperalgesic Effect of Polyalthic Acid and Naproxen
2.5. Synergistic Interaction between Polyalthic Acid and Naproxen
2.6. Anti-Inflammatory Effect of Polyalthic Acid
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Drugs
4.3. Formalin Test
4.4. L5/L6 Spinal Nerve Ligation
4.5. Thermal Hyperalgesia
4.6. Isobologram
4.7. Carrageenan-Induced Paw Edema
4.8. Experimental Design
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Combination | Oral Dose (mg/kg) | ||
---|---|---|---|
Polyalthic Acid | Naproxen | Total Dose in the Combination | |
1 | 3.2 | 0.6 | 3.8 |
2 | 6.4 | 1.3 | 7.7 |
3 | 12.7 | 2.5 | 15.2 |
4 | 25.4 | 5.0 | 30.4 |
5 | 50.8 | 10 | 60.8 |
Drug | ED50 (mg/kg) |
---|---|
Naproxen | 20.1 ± 4.8 |
Polyalthic acid | 101.6 ± 30.9 |
Theoretical ED50 of the polyalthic acid–naproxen combination | 60.9 ± 15.7 CI (32.4–114.3) |
Experimental ED50 of the polyalthic acid-–naproxen combination | 2.4 ± 0.02 * CI (0.9–6.6) |
Interaction index (γ) | 0.04 ± 0.02 CI (0.02–0.10) |
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Rodríguez-Silverio, J.; Sánchez-Mendoza, M.E.; Rocha-González, H.I.; Reyes-García, J.G.; Flores-Murrieta, F.J.; López-Lorenzo, Y.; Quiñonez-Bastidas, G.N.; Arrieta, J. Evaluation of the Antinociceptive, Antiallodynic, Antihyperalgesic and Anti-Inflammatory Effect of Polyalthic Acid. Molecules 2021, 26, 2921. https://doi.org/10.3390/molecules26102921
Rodríguez-Silverio J, Sánchez-Mendoza ME, Rocha-González HI, Reyes-García JG, Flores-Murrieta FJ, López-Lorenzo Y, Quiñonez-Bastidas GN, Arrieta J. Evaluation of the Antinociceptive, Antiallodynic, Antihyperalgesic and Anti-Inflammatory Effect of Polyalthic Acid. Molecules. 2021; 26(10):2921. https://doi.org/10.3390/molecules26102921
Chicago/Turabian StyleRodríguez-Silverio, Juan, María Elena Sánchez-Mendoza, Héctor Isaac Rocha-González, Juan Gerardo Reyes-García, Francisco Javier Flores-Murrieta, Yaraset López-Lorenzo, Geovanna Nallely Quiñonez-Bastidas, and Jesús Arrieta. 2021. "Evaluation of the Antinociceptive, Antiallodynic, Antihyperalgesic and Anti-Inflammatory Effect of Polyalthic Acid" Molecules 26, no. 10: 2921. https://doi.org/10.3390/molecules26102921