Structure–Activity Relationship Assessment of Sophorolipid Ester Derivatives against Model Bacteria Strains
Abstract
:1. Introduction
2. Results and Discussion
2.1. In Vitro Antibacterial Efficacy and Structure–Activity Relationship of SL Derivatives
2.2. Time–Kill Studies
2.3. Membrane Depolarization Assay
2.4. In Vitro Cytotoxicity
3. Materials and Methods
3.1. Materials and Reagents
3.2. SL Synthesis
3.3. Bacteria Strain Culture and Antibacterial Testing
3.4. Time–Kill Studies
3.5. Cytotoxicity Study
3.6. Dye Leakage Experiment
4. Conclusions
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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SLs | MIC (µM) | TI | ||||
---|---|---|---|---|---|---|
B. cereus1 | B. subtilis1 | S. aureus1 | L. innocua2 | E. coli1 | ||
Lactonic SL 3 | 25 | 12 | 25 | 50 | >200 | 2 |
SL-methyl ester | 50 | 50 | 200 | 100 | >200 | >8 |
SL-ethyl ester | 50 | 50 | 200 | 50 | >200 | 6 |
SL-propyl ester | 25 | 25 | 100 | 25 | >200 | 16 |
SL-butyl ester | 12 | 12 | 100 | 12 | >200 | 33 |
SL-pentyl ester | 12 | >200 | >200 | 12 | >200 | 17 |
SL-hexyl ester | >200 | >200 | >200 | >200 | >200 | - |
SL-octyl ester | >200 | >200 | >200 | >200 | >200 | - |
Streptomycin | 12 | 12 | 3 | 24 | 12 | - |
SLs | MIC (µM) | |||
---|---|---|---|---|
B. Cereus1 | B. Subtilis1 | L. innocua2 | E. coli1 | |
ESL-(6′OH, 6″OH) 3 | 50 | 50 | 50 | >200 |
ESL-(6′Ac, 6″OH) | 25 | 25 | 25 | >200 |
ESL-(6′OH, 6″Ac) | 25 | 25 | 25 | >200 |
ESL-(6′Ac, 6″Ac) | 12 | >200 | >200 | >200 |
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Totsingan, F.; Liu, F.; Gross, R.A. Structure–Activity Relationship Assessment of Sophorolipid Ester Derivatives against Model Bacteria Strains. Molecules 2021, 26, 3021. https://doi.org/10.3390/molecules26103021
Totsingan F, Liu F, Gross RA. Structure–Activity Relationship Assessment of Sophorolipid Ester Derivatives against Model Bacteria Strains. Molecules. 2021; 26(10):3021. https://doi.org/10.3390/molecules26103021
Chicago/Turabian StyleTotsingan, Filbert, Fei Liu, and Richard A. Gross. 2021. "Structure–Activity Relationship Assessment of Sophorolipid Ester Derivatives against Model Bacteria Strains" Molecules 26, no. 10: 3021. https://doi.org/10.3390/molecules26103021