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Article

Phosphonodithioester–Amine Coupling as a Key Reaction Step for the Design of Cationic Amphiphiles Used for Gene Delivery

by
Montassar Khalil
1,
Alexis Hocquigny
2,
Mathieu Berchel
1,
Tristan Montier
2,3 and
Paul-Alain Jaffrès
1,*
1
Univ Brest, CNRS, CEMCA UMR 6521, 6 Avenue Victor Le Gorgeu, 29238 Brest, France
2
Univ Brest, INSERM, EFS, UMR 1078, GGB-GTCA, 29200 Brest, France
3
CHRU de Brest, Service de Génétique Médicale et Biologie de la Reproduction, Centre de Référence des Maladies Rares “Maladies Neuromusculaires”, 29200 Brest, France
*
Author to whom correspondence should be addressed.
Molecules 2021, 26(24), 7507; https://doi.org/10.3390/molecules26247507
Submission received: 8 November 2021 / Revised: 6 December 2021 / Accepted: 7 December 2021 / Published: 11 December 2021
(This article belongs to the Special Issue Synthesis of Amphiphilic Compounds and Applications)
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Abstract

:
A convergent synthesis of cationic amphiphilic compounds is reported here with the use of the phosphonodithioester–amine coupling (PAC) reaction. This versatile reaction occurs at room temperature without any catalyst, allowing binding of the lipid moiety to a polar head group. This strategy is illustrated with the use of two lipid units featuring either two oleyl chains or two-branched saturated lipid chains. The final cationic amphiphiles were evaluated as carriers for plasmid DNA delivery in four cell lines (A549, Calu3, CFBE and 16HBE) and were compared to standards (BSV36 and KLN47). These new amphiphilic derivatives, which were formulated with DOPE or DOPE-cholesterol as helper lipids, feature high transfection efficacies when associated with DOPE. The highest transfection efficacies were observed in the four cell lines at low charge ratios (CR = 0.7, 1 or 2). At these CRs, no toxic effects were detected. Altogether, this new synthesis scheme using the PAC reaction opens up new possibilities for investigating the effects of lipid or polar head groups on transfection efficacies.

Graphical Abstract

1. Introduction

Cationic amphiphilic compounds form liposomes in water and can interact with nucleic acids (e.g., pDNA, siRNA, mRNA) to produce lipoplexes after full reorganization of the liposomal supramolecular assemblies [1]. This self-reorganization occurs thanks to both ionic interactions and the self-assembly of their lipid chains, limiting their interactions with water [2]. Since the pioneering works of Felgner [3], many cationic amphiphilic structures have been proposed [4], with variations in the structure of the linker [5], the lipid chains [6] or the polar head group [7]. The final goal of all these cationic amphiphiles consists of producing supramolecular assemblies featuring tunable stability in order to be sufficiently stable during the carrying process, while also permitting cargo release in the cytosol after cell internalization, which usually occurs via an endocytosis pathway [8]. Accordingly, different types of liposomes responding to variations in pH [9,10], to reducing agents [11,12] or to enzymatic reactions [13] have been designed. These amphiphilic compounds have been used for in vitro or in vivo nucleic acid delivery assays (pDNA [14], mRNA [15]) aiming to address a broad panel of applications including vaccination [16], tendon healing [17], lung transfection [18,19] or cancer therapy [20,21]. Other type of cationic amphiphiles have been designed with the aim of controlling their molecular shape, which is, according to the Israelachvili theory [22], a key parameter for controlling the type of supramolecular self-assembly. Following this line, we have reported the synthesis of phospholipids using commercially available branched lipid chains [23] or including branched lipid chains prepared via the photo-thiol-ene reaction [24]. These cationic amphiphiles form inverted hexagonal aggregates that promote lipid fusion and favor the transfection process. With the aim of tuning the stability of the supramolecular assemblies, we have also incorporated a thiophosphoramide moiety in order to adjust the robustness of the hydrogen bonds networks present in liposomes and lipoplexes [25]. One important feature for the design of amphiphilic compounds is the versatility of their synthesis in order to explore new structures and to evaluate their transfection capacity. Moreover, the simplicity of the synthesis (e.g., limiting the number of steps) is a key parameter for facilitating the translation of in vitro to in vivo studies via the production of amphiphilic compounds at a multi-gram scale [26]. Accordingly, a convergent synthesis that allows for a better synthesis efficacy is highly desired. With regard to this, the coupling of the lipid moieties to the polar head group in the final step or close to the final step is an attractive strategy. Different click reactions including thiol-ene [27], thiol-yne [28] or copper alkyne-azide coupling—CuAAC [29] have been previously reported for construction of amphiphilic compounds used to prepare vesicles, including some of that were used for transfection. We report here a new convergent synthesis scheme for the preparation of cationic amphiphiles, in which the key step is a phosphonodithioester–amine coupling reaction (PAC reaction). The PAC reaction was initially reported by Masson et al., 1994 [30] as a new method for the preparation of phosphonothioamide. Following this, two studies reported its application for the functionalization of chitosan [31] and for the functionalization of the surface of liposomes [32]. This reaction was also extended to fluorinated phosphodithioacetate [33]. The interest of this reaction comes from its achievement under mild conditions and without any catalyst. We report here for the first time the use of the PAC reaction for the preparation of two cationic amphiphilic compounds 4a–b (Figure 1) that were used for in vitro transfection assays in four cell lines (A549, Calu3, CFBE, 16HBE). The transfection efficacies were compared to two reference compounds KLN47 and BSV36 (Figure 1), permitting us to conclude both the influence of the lipid chain and the effect of the phosphono-thioamide moiety on the transfection efficacy.

2. Materials and Methods

2.1. Materials

All reagents, including oleyl alcohol (technical grade, 85%) and 2-decyl-tetradecan-1-ol (97%), were purchased from commercial sources and used as received (Aldrich, TCI). Solvents (THF, CH2Cl2) were dried with an MBRAUN solvent purification system and kept under a molecular sieve. Milli-Q water was used as solvent for the liposomal formulations. NMR spectroscopy data were recorded with a Bruker Avance 400 (1H: 400.0 MHz, 31P: 161.9 MHz, T = 293 K) or a Bruker Avance 500 (13C: 125.7 MHz, T = 293 K). The 13C NMR spectra were recorded with a J-MOD sequence (J-modulated spin-echo). The chemical shifts are reported in δ[ppm] (multiplicity, coupling constant J, number of protons) relative to the solvent residual signal as the internal standard. For 31P NMR, an external reference (H3PO4; δ = 0 ppm) was used. The coupling constants are given in Hertz [Hz]. Mass spectra were recorded with the autoflex III Brucker MALDI-TOF mass spectrometer (α-Cyano-4-hydroxycinnamic acid (HCCA) or a SYNAPT XS High Resolution Mass Spectrometer (ESI-qTOF). IR were recorded using attenuated total reflection mode (ATR) on a Bruker platinum ATR VERTEX 70 IR spectrometer. The mean particle diameter and zeta potential (ξ) were measured using a Malvern Zetasizer Nano ZS at 25 °C using 1 cm disposable polystyrene cuvettes. KLN47 [34] and BSV36 [35] were synthesized following reported procedures.

2.2. Synthesis and Characterization

The synthesis scheme is reported in Figure 2. This sequence starts with the synthesis of lipid-phosphonodithioformiates 2a–b in two steps involved in the PAC reaction to yield 3a–b. Then, in the final step, the methylation of the tertiary amine produced the cationic amphiphiles 4a–b. The NMR spectra are accessible in the supporting information (SI).

2.2.1. Dioleylphosphite 1a

A mixture of oleyl alcohol (3 g, 11.2 mmol) and diphenylphosphite (1.25 g, 5.3 mmol) was placed in a Kugelrohr distillation apparatus. The mixture was heated at 140 °C under reduced pressure (4.10−2 mbar) for 4 h. Phenol, which was sublimed during this heating period, was discarded. Then, the temperature was increased to 180 °C (4.10−2 mbar) for 1 h to remove the excess oleyl alcohol. The undistilled compound 1a was isolated as a pale-yellow oil (3.0 g; 97% yield). 1H NMR (400.0 MHz, CDCl3): δ = 6.79 (d, 1JHP = 692, 1H), 5.36–5.33 (m, 4H), 4.09–4.03 (m, 4H), 2.03–1.98 (m, 8H), 1.70–1.66 (m, 4H), 1.30–1.25 (m, 49H), 0.88 (t, 3JHH = 6.9, 6H); 31P{1H} NMR (161.9 MHz, CDCl3): δ = 9.12; 13C{1H} J-MOD NMR (125.7 MHz, CDCl3): δ = 132.70 and 132.45 (2C, HC=CH), 68.51 (d, 2JCP = 5.7, CH2-O), 35.32 (CH2), 34.62 (CH2), 33.13 (d, 3JCP = 6.0, CH2-CH2-O), 32.48–31.81 (CH2 fatty chains), 29.93 (CH2), 29.89 (CH2), 28.21 (CH2), 25.39 (CH2), 16.82 (CH3).

2.2.2. Bis(2-decyltetradecyl)phosphite 1b

The same protocol used for 1a was applied. Diphenylphosphite (0.99 g, 4.2 mmol), 2-decyltetradecan-1-ol (3.00 g, 8.45 mmol). Compound 1b was isolated as a colorless oil (3.0 g; 94% yield). 1H NMR (400.0 MHz, CDCl3): δ = 6.81 (d, 1JHP = 691, 1H), 4.00–3.97 (m, 4H), 1.65–1.64 (m, 4H), 1.34–1.24 (m, 83H), 0.91 (t, J = 6.8, 12H); 31P{1H} NMR (161.9 MHz, CDCl3): δ = 9.61; 13C{1H} J-MOD NMR (125.7 MHz, CDCl3): δ = 70.80 (d, 2JCP = 6.1, CH2-O), 41.41 (d, 3JCP = 6.5, CH-CH2-O), 34.64 (CH2), 33.43 (CH2), 32.66 (CH2), 32.38–32.33 (CH2 fatty chains), 32.07 (CH2), 29.36 (CH2), 25.40 (CH2), 16.80 (CH3); Maldi TOF (matrix: HCCA): [M+Na]+ calculated for [C48H99O3P+Na]+ = 777.72; observed [M+Na]+ = 777.82.

2.2.3. Methyl Dioleylphosphonodithioformate 2a

To a mixture of NaH (107 mg, 4.46 mmol) in 15 mL of anhydrous tetrahydrofuran placed in a Schlenk flask under nitrogen, a solution of phosphite 1a (2 g, 3.43 mmol) solubilized in 15 mL of anhydrous THF was added under stirring at room temperature. At the end of the addition, the mixture was heated to 54 °C for 4 h (a limpid solution was formed). The 31P NMR monitoring (D2O in capillary used as an internal standard) revealed a peak at 152 ppm for the phosphite sodium salt. Then, the solution was cooled down to −78 °C and treated with anhydrous carbon disulfide (1.56 g, 1.25 mL, 20.6 mmol). The mixture was stirred for 2 h at room temperature and methyl iodide (536 mg, 3.77 mmol) was subsequently added. After stirring for an additional 2 h, the mixture was washed with a saturated aqueous solution of ammonium chloride and was extracted twice with 40 mL of ethyl acetate. The organic phase was washed with brine, dried over anhydrous sodium sulfate, filtrated and concentrated. A purification by chromatography on silica gel (eluent of n-hexane/ethyl acetate from 100:0 to 70:30 in volume) gave 2a as a purple oil, obtained with a 20% yield (450 mg). 1H NMR (400.0 MHz, CDCl3): δ = 5.38–5.35 (m, 4H), 4.24–4.16 (m, 4H), 2.72 (s, 3H), 2.04–2.01 (m, 8H), 1.75–1.69 (m, 4H), 1.29–1.28 (m, 56H), 0.90 (t, 3JHH = 6.8, 6H); 31P{1H} NMR (161.9 MHz, CDCl3): δ = −1.69; 13C{1H} J-MOD NMR (125.7 MHz, CDCl3): δ = 229.58 (d, 1JCP = 176.6, C=S), 129.96 + 129.74 (CH2-CH=CH-CH2), 68.57 (d, 2JCP = 6.8, CH2-O-P), 31.91 to 22.68 (CH2 fatty chains), 19.20 (S-CH3), 14.11 (CH3-CH2); Maldi TOF (matrix: HCCA): [M+Na]+ calculated for [C38H73O3PS2Na]+ = 695.46; observed [M+Na]+ = 695.72.

2.2.4. Methyl Bis-(2-decanyltetradecyl)phosphonodithioformate 2b

Same protocol as for 2a. NaH (83 mg, 3.44 mmol), phosphite 1b (2 g, 2.65 mmol). Intermediate 31P NMR (D2O in a capillary used as an internal standard): 152 ppm; carbon disulfide (1.21 g, 960 µL, 15.9 mmol), methyl iodide (414 mg, 180 µL, 2.92 mmol). Purification by chromatography on silica gel (eluent n-hexane/ethyl acetate: 100:0 to 70:30). Compound 2b was isolated in 20% yield (400 mg). 1H NMR (400.0 MHz, CDCl3): δ = 4.12–4.09 (m, 4H), 2.72 (s, 3H), 1.67 (m, 2H), 1.33–1.28 (m, 93H), 0.91 (t, 3JHH = 6.8, 13H); 31P{1H} NMR (161.9 MHz, CDCl3): δ = −1.85; 13C{1H} J-MOD NMR (125.7 MHz, CDCl3): δ = 229.64 (d, 1JCP = 177.0, C=S), 70.91 (d, 2JCP = 7.4, CH2-O-P), 38.74 (d, 3JCP = 6.4, CH-CH2-O-P), 31.95 to 22.71 (CH2, fatty chains), 19.11 (S-CH3), 14.12 (CH3-CH2); Maldi TOF (matrix: HCCA): [M+Na]+ calculated for [C50H101O3PS2Na]+ = 867.68; observed [M+Na]+ =867.87.

2.2.5. Dioleyl ((2-(dimethylamino)ethyl)carbamothioyl)phosphonate 3a

To a solution of 2a (200 mg, 0.297 mmol, 1 eq.) in 10 mL of CHCl3, N,N-dimethylethylenediamine (28.81 mg, 35 µL, 0.326 mmol, 1.1 eq.) was slowly added. The reaction was stirred at room temperature for 2 h. The excess amine was removed under vacuum. The compound was purified by chromatography on silica gel (CH2Cl2/MeOH: 90/10 (v/v)) to produce 3a as a yellow wax (121 mg; 57% yield). 1H NMR (400.0 MHz, CDCl3): δ = 5.37–5.32 (m, 4H), 4.21–4.13 (m, 4H), 3.69 (t, 3JHH = 4.6, NH-CH2-, 2H), 2.60 (t, 3JHH = 5.9, N(CH3)2-CH2-, 2H), 2.27 (s, 6H), 2.01–1.98 (m, 8H), 1.71–1.68 (m, 4H), 1.36–1.25 (m, 52H), 0.87 (t, 3JHH = 6.8, 6H); 31P{1H} NMR (161.9 MHz, CDCl3): δ = −1.20, 13C{1H} J-MOD NMR (125.7 MHz, CDCl3): δ = 193.07 (d, 1JCP = 182.4, C=S), 129.98 + 129.77 (CH2-CH=CH-CH2), 68.87 (d, 2JCP = 7.0, CH2-O-P), 55.67 (CH2-N(CH3)2), 44.95 (N(CH3)2-CH2-), 42.39 (d, 3JCP = 7.85, P-CS-NH-CH2-), 31.91 to 22.69 (m, CH2 fatty chains), 14.12 (CH3-CH2); Maldi TOF (matrix: HCCA): [M+H]+ calculated for [C41H82N2O3PS]+ = 713.58; observed [M+H]+ =713.74.

2.2.6. Bis(2-decanyltetradecyl) ((2-(dimethylamino)ethyl)carbamothioyl)phosphonate 3b

Same protocol as for 3a. 2b (100 mg, 0.118 mmol, 1 eq.), N,N-dimethylethylenediamine (11.47 mg, 14 µL, 0.13 mmol, 1.1 eq.). After purification by chromatography on silica gel (CH2Cl2/MeOH: 90/10 (v/v)), 3b was isolated as a yellow wax (51 mg, 49% yield). 1H NMR (400.0 MHz, CDCl3): δ = 4.10–4.04 (m, 4H), 3.67 (t, 3JHH = 5.9, NH-CH2-, 2H), 2.57 (t, 3JHH = 5.62, N(CH3)2-CH2-, 2H), 2.25 (s, 6H), 1.62 (m, 2H), 1.29–1.24 (m, CH2 fatty chains), 0.87 (t, 3JHH = 6.8, 12H); 31P{1H} NMR (161.9 MHz, CDCl3): δ = −1.66; 13C{1H} J-MOD NMR (125.7 MHz, CDCl3): δ = 192.95 (d, 1JCP = 182.9, C=S), 71.23 (d, 2JCP = 7.5, CH2-O-P), 65.70 (-CH2-N(CH3)3-), 55.67 (CH2-NH), 44.98 (N(CH3)3-), 38.71 (d, 3JCP = 6.3, CH-CH2-O-P), 31.93 to 29.37 (m, CH2 fatty chains), 22.69 (-CH2-CH3) 14.11 (CH2-CH3); Maldi TOF (matrix: HCCA): [M+H]+ calculated for [C53H110N2O3PS]+ = 885.80; observed [M+H]+ = 885.93.

2.2.7. 2-((Dioleyloxyphosphoryl)methanethioamido)-N,N,N-trimethylethan-1-aminium iodide 4a

Methyl iodide (200 mg, 1.4 mmol, 86 µL, 10 eq.) was added to a solution of 3a (100 mg, 0.14 mmol, 1 eq.) in CHCl3 (5 mL). The solution was stirred overnight at 20 °C. The solvent and the excess of methyl iodide were evaporated under vacuum. After purification by chromatography on silica gel (CH2Cl2/MeOH: 100/0 to 90/10 (v/v)), 4a was isolated as a yellow wax (76 mg; 75% yield).1H NMR (400.0 MHz, CDCl3): δ = 5.32–5.29 (m, 4H), 4.38 (CH2-N(CH3)3, 2H), 4.18–4.15 (m, CH2-O-P, 4H), 4.00 (t, 3JHH = 6.3, NH-CH2-, 2H), 3.45 (s, N(CH3)3, 9H), 1.98–1.95 (m, 4H), 1.70–1.67 (m, 4H), 1.25–1.22 (m, fatty chains), 0.84 (t, 3JHH = 6.8, 6H); 31P{1H} NMR (161.9 MHz, CDCl3): δ = −2.34; 13C{1H} J-MOD NMR (125.7 MHz, CDCl3): δ = 196.18 (d, 1JCP = 186.0, C=S), 130.01 + 129.77 (CH2-CH=CH-CH2), 69.42 (d, 2JCP = 7.0, CH2-O-P), 62.83 (CH2-N(CH3)3), 54.63 (N(CH3)3-), 38.82 (d, 3JCP = 8.6, P-CS-NH-CH2-), 29.80 to 27.25 (m, CH2 fatty chains), 14.15 (CH3-CH2).

2.2.8. 2-((Bis(2-decanyltetradecyloxy)phosphoryl)methanethioamido)-N,N,N-trimethylethan-1-ammonium iodide 4b

Same protocol as for 4a. 3b (51 mg, 0.058 mmol, 1 eq.), methyl iodide (82 mg, 0.576 mmol, 35 µL, 10 eq). After purification by chromatography on silica gel (CH2Cl2/MeOH: 90/0 to 10 (v/v)), 4a was isolated as a wax (44 mg; 85% yield). 1H NMR (400.0 MHz, CDCl3): δ = 4.42 (t, 3JHH = 5.9, NH-CH2-, 2H), 4.12–4.05 (m, 4H), 4.03 (t, 3JHH = 6.4, N(CH3)2-CH2-, 2H), 3.48 (s, 9H), 1.81 (m, 2H), 1.68 (m, 2H), 1.30–1.25 (m, CH2 fatty chains), 0.87 (t, 3JHH = 6.9, 12H); 31P{1H} NMR (161.9 MHz, CDCl3): δ = −2.24; 13C{1H} J-MOD NMR (125.7 MHz, CDCl3): δ = 196.24 (d, 1JCP = 184.5, C=S), 72.03 (d, 2JCP = 7.5, CH2-O-P), 62.68 (-CH2-N(CH3)3-), 54.62 (N(CH3)3-), 38.73 (d, 3JCP = 6.1, CH-CH2-O-P), 31.95 (NH-CH2-), 30.55 to 29.41 (m, CH2 fatty chains), 14.11 (CH2-CH3); HRMS (ESI-qTOF): m/z calculated for [C54H112N2O3PS]+ [M]+ = 899.8131; observed [M]+ = 899.8121.

2.3. Preparation of Liposomes

The cationic lipids BSV36 and KLN47 (used as references) were formulated as previously reported [35]. The new compounds 4a and 4b were formulated as liposomal solutions with a helper lipid—1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) and cholesterol (Chol)—by hydration of a lipid film or by ethanolic injection, as summarized in Table 1. Briefly, for LF1–2 formulations (1.5 µmol of 4a or 4b), 4a was taken from a stock solution in chloroform and a colipid (1.5 µmol of DOPE or/and cholesterol taken from a stock solution in chloroform) were placed in a 10 mL round-bottom flask. The chloroform was evaporated under vacuum with a rotary evaporator. The resulting lipid film was hydrated with 1 mL of water (MilliQ water) and left at 4 °C for one night. Then, the solution was vortexed and sonicated (30 min at 45 °C.) to produce a transparent solution.
For LF3 formulation, the ethanolic injection method was applied because the method using the hydration of a lipid film was unsuccessful. Briefly, a mixture of 4b (1.5 µmol) and DOPE (1.5 µmol) in a small volume of ethanol (1% of the final volume of the liposomal solution) was slowly added to an aqueous solution (1 mL) under stirring. In the final step, ethanol was removed by evaporation at 45 °C under vacuum.

2.4. Size and Zeta Measurements

An aliquot of 50 µL of the liposomal solutions was diluted in 950 µL of Milli-Q water. This solution was transferred into a MALVERN DTS1060 cuvette for measurements.
The same procedure was applied to record the size and zeta potential of the lipoplexes at different charge ratios (CRs). A total of 0.625 μg of plasmid pGM144 was used to prepare 50 μL of complex solutions.

2.5. DNA Complexation

This procedure was similar to a previously reported protocol [36]. Liposomes were sonicated for 10 min at 38 °C before use, while the concentration of the pDNA solution was quantified with the NanoDrop ND-1000. Briefly, lipoplexes were prepared by mixing pDNA (0.25 µg pGM144 for 20 µL of complexes solutions; pGM144 is a plasmid coding for luciferase) with each liposomal solution in water at different charge ratios (CRs) from 0.7 to 4 (CRs are defined by the ratio of the number of cationic charges coming from the cationic amphiphiles divided by the number of negative charges coming from the phosphate moieties of the plasmid). All mixtures were incubated at room temperature for 30 min. The complexes were subjected to electrophoresis in 0.8% agarose gel at 100 V, 90 mA for 20 min. The gel was stained with BET (ethidium bromide) and then visualized using a UV transilluminator.
As shown in Figure 3, the DNA complexation of LF1–LF3 requires a charge ratio of 2 or 4 to obtain a full complexation of pDNA. When partial complexation is observed (CR = 0.7, 1 and 2), the intensity of the free DNA appears to correlate with the CR, with progressive complexation observed with increases in the CR.

2.6. In Vitro Transfection Efficacies

This procedure was similar to a previously reported protocol (Le Corre et al., 2014 [37]). Briefly, cells were grown in EMEM (16HBE and CFBE) or DMEM (A549, Calu-3), both supplemented with 10% fetal bovine serum, 1% antibiotic and 1% L-glutamine at 37 °C in a humidified atmosphere containing 5% CO2. Cells were seeded onto a 96-well plate at a density of 20,000 to 40,000 cells per well for 24 h before transfection and incubated at 37 °C overnight. Complexes (including 0.25 µg of plasmid/well) were added dropwise to each well; the reference compounds BSV36 and KLN47 were used as positive transfection controls, whereas naked DNA was used as a negative control. After 24 h, the culture medium was removed and the cells were lysed with Passive Lysis Buffer (Promega) in order to be assayed for luciferase expression using a chemoluminescent assay (Luciferase Assay System, Promega). The total protein content of the cell lysate was determined using the BC assay kit (Uptima). Data are expressed as relative light units (RLU) per milligram of total protein (mean ± SD with n = 3), as depicted in Figure 4.

2.7. Cell Viability

This procedure was similar to a previously reported protocol (Delbeke et al., 2016 [38]). Briefly, the ATP content, which reflects the number of living cells (transfected or not) in culture, was determined using the ViaLight kit (Lonza, Basel, Switzerland) 24 h after transfection. This assay was used as recommended by the manufacturer. The results (Figure 5) were expressed as percentages relative to the viability of untransfected cells, which was used as the reference (100% cell viability).

3. Results and Discussion

The synthesis of the new cationic lipids is depicted in Figure 2. Regarding the choice of the lipid chains, we selected oleyl chains or the branched lipid chain 2-decyl-tetradecanyl because they proved their efficacy when incorporated in the structure of other cationic amphiphiles [23,35]. Previous publications have indeed observed that oleyl chains increase the fluidity of self-assemblies thanks to the presence of their carbon–carbon double bond possessing a Z-configuration. This structural feature introduces a kink in the lipid chain that disturbs the lipid packing and increases fluidity, as previously reported [39]. In the case of branched lipid chains, the ramification results in a larger volume of hydrophobic chains that contribute to the formation of a cone-shaped amphiphilic structure that favors non-lamellar self-assemblies and produces efficient gene carriers [23]. Their synthesis starts with a transesterification reaction involving a lipid alcohol and diphenylphosphite. This reaction can be achieved on large scale (10 g) and with high yields (>95%) in a Kugelrohr apparatus. In these conditions, phenol was removed from the products 1a–b (Figure 2) by sublimation. Then, these phosphites were used for the preparation of the methylphosphonodithioformiates 2a–b by adapting the method of Grisley [40]. The presence of the lipid chains reduced the reactivity of the phosphite. Accordingly, an efficient deprotonation required heating of the phosphite in the presence of sodium hydride in dry THF (reflux). Then, there was the addition of carbon disulfide at a low temperature, in order to avoid a desulfuration reaction [41], and finally, the methylation step produced the expected phosphonodithioformiates 2a–b at modest yields (20%). This low yield was accounted for by the formation of side products (visible by 31P NMR) that imposed the loss of some of the expected product that was eluted in the same fractions as the by-products. Then, the phosphonodithioester–amine coupling (PAC) reaction was achieved by mixing 2a–b with N,N-dimethylethylenediamine. The color of the reaction changed immediately from deep purple to yellow when N,N-dimethylethylenediamine was added—indicating a fast reaction, as previously reported for this type of reaction [32]. The resulting phosphonothioamides 3a–b were methylated to produce the cationic amphiphilic compounds 4a–b. Then, 4a–b were formulated for the preparation of liposomal solutions. We observed that their formulation alone by either hydration of a lipid film or ethanol-injection methods produced very large aggregates with very high values on the PolyDispersity Index (PDI). In consequence, we associated 4a–b with DOPE (1/1 molar ratio with either 4a or 4b) or DOPE/cholesterol as co-lipids in order to facilitate the formulation step. The resulting formulations were identified as LF1, LF2 and LF3 (Table 1). It must be noted that the incorporation of cholesterol (LF2 vs. LF1) reduced the transfection efficacy (as discussed later); as a consequence, the addition of cholesterol into LF3 was not considered in this study. These formulations were characterized by dynamic light scattering (DLS) to determine their size and zeta potential. The size of the aggregates (Table 2) was distributed over a quite narrow range of values (from 194 to 229 nm), and the zeta potentials were clearly positive—as expected for formulations including cationic amphiphilic compounds (+57 to +79 mV). After storing these formulations for 5 months, new analyses (Table S1) indicated that these formulations were still suitable for use as transfection agents (size < 200 nm; positive zeta potentials). The sizes and zeta potentials of the lipoplexes were also recorded (Table S2). It was observed that the size of the lipoplexes (depending on the charge ratios) were either similar or slightly smaller. For the zeta potential, positive values were observed at CR = 2 or 4 for all the formulations except for LF3. Indeed, for LF3, a slightly negative zeta potential (−13 to −10 mV) was observed at the different charge ratios. This negative charge could be due to a different type of supramolecular assembly. Branched lipid chains are indeed prone to adopting inverted hexagonal assemblies [23]. The complexation properties of these formulations were evaluated using gel electrophoresis of the lipoplexes, prepared in water at different charge ratios (from 0.7 to 4; Figure 3). It appeared that LF1 fully compacted pDNA at CR = 2, whereas LF2 required CR = 4 to produce full complexation. LF3 was less efficient at compacting pDNA, since even at CR 4 pDNA was only partly compacted. This behavior could result from non-lamellar packing [23]. These formulations were then tested as gene carriers for the transfection of four cell lines (A549, Calu3, CFBE, 16HBE), and with pGM144 plasmid [42] used as a gene reporter (a plasmid encoding luciferase). For these experiments, we used BSV36 and KLN47 as references because we have previously reported their transfection efficacies [35]. KLN47 has also been previously compared to other commercial kits of transfection [43]. The new cationic amphiphiles 4a and 4b formulated with DOPE (LF1 and LF3; Table 1), or with DOPE and cholesterol (LF2; Table 1), were very efficient most of the time at transfecting all the cell lines, and were therefore good candidates compared to other nucleic acid carriers [44] for use in further developmental studies. The most remarkable results came from the formulation LF1, which proved to be very efficient at transfecting the four cell lines tested at relatively low charge ratios (CRs = 0.7 to 2; Figure 4). The effects of the transfection conditions on cell viability were studied and the results are depicted in Figure 5. Overall, the formulations exhibited low toxicity at CR = 0.7, 1 and 2. Some toxicity was observed at CR 4 for BSV36 in some cell lines (A549, Calu3, 16HBE). However, at the CRs efficient for transfection (CR = 0.7, 1 or 2), the most efficient formulations—LF1 and LF2—were not toxic under the tested conditions. These results confirm the interest of the formulations LF1 and LF2, which include cationic amphiphilic compounds that are prepared using the PAC reaction in order to include in their molecular structures a phosphonothioamide moiety.

4. Conclusions

For the first time, cationic amphiphilic compounds have been prepared using a phosphonodithioester–amine coupling (PAC) reaction. The final cationic amphiphilic compounds 4a–b were isolated in a four-step synthesis scheme in sufficient quantities for their evaluation as plasmid DNA carriers. The interest of the PAC reaction comes from its rapidity and from its efficacy without the requirement of any catalyst. These cationic amphiphilic compounds were formulated in the presence of DOPE or DOPE/cholesterol to produce well-defined liposomal solutions (size close to 200 nm). These two cationic amphiphilic compounds included in their structure either oleyl chains or branched lipid chains that had previously proved their efficacy when incorporated into other type of cationic amphiphilic structures. The formulations, including 4a–b, feature different capacities for compacting pDNA. Among these formulations, the one featuring the highest capacity to compact pDNA (LF1) was also the most efficient formulation for the transfection of four cell lines (A549, Calu3, CFBE, 16HBE). Compound 4a, formulated with either DOPE or DOPE/cholesterol (LF1 or LF2), was the most efficient formulation for transfecting the four cell lines. The most remarkable results were that LF1 and LF2 were efficient at low charge ratios (at charge ratios where the complexation of pDNA was incomplete), suggesting that such partial complexation could be helpful for high in vitro transfection efficacies—likely by facilitating the release of the plasmid from the endosome to the cytosol. The side effects induced by these formulations were evaluated by determining cell viability. The most efficient formulations, LF1 and LF2, exhibited no toxicity at the charge ratio where they were the most efficient (CR = 0.7, 1 or 2). The influence of the lipid chain on the transfection efficacy was in favor of the oleyl chains (LF1 and LF2). With the cationic amphiphile-featuring branched lipid chain (LF3), lower transfection efficiencies were observed—except for CFBE cell line. Altogether, the cationic amphiphilic 4a compounds formulated with DOPE (LF1) produced very promising results in terms of transfection efficacies, coupled with the absence of toxic effects. The synthetic approach, based on the PAC reaction, opens up new possibilities for the modulation of both the polar head group and the hydrophobic domain.

Supplementary Materials

The following are available online, in the supplementary materials part: Figures S1–S35 correspond to NMR and MS spectra (1H, 31P, 13C, MALDI TOF or ESIqTOF MS spectra) for the compounds 2–4/a–b, Table S1 “Sizes and zeta potentials of the liposomes at t = 0 and 5 months later.”, Table S2 “Sizes and zeta potentials of the lipoplexes at different charges ratios”, Table S3 “Fraction of complexed DNA and free DNA after complexation process with the cationic lipids determined by densitometry analysis on the electrophoresis gel”, Figure S36 “Proportion of complexed and free DNA after complexation process with the cationic lipids determined by densitometry analysis on the electrophoresis gel.”

Author Contributions

Conceptualization, M.B., T.M. and P.-A.J.; methodology, M.K. and A.H.; validation, M.B., T.M. and P.-A.J.; writing—original draft preparation, P.-A.J.; writing—review and editing, all. All authors have read and agreed to the published version of the manuscript.

Funding

This research was funded by grants from the ANR—Agence Nationale de la Recherche (project no. ANR-17-CE18-0015-03, “monop-DNA-Nanoparticules Virus-Inspirées pour transfert de gènes”; ANR, Paris, France); the Association de Transfusion Sanguine et de Biogénétique Gaétan Saleün (Brest, France); and the Région Bretagne (France). M.K. and A.H. were recipient of a PhD fellowship from the French Ministry of Higher Education, Research and Innovation.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Acknowledgments

The Service Général des Plateformes, Animalerie Commune (Brest, France), RMN-RPE et spectrométrie de masse and SynNanoVect (Brest-Rennes, France) are also acknowledged.

Conflicts of Interest

The authors declare that there are no conflict of interest, no competing interests and nothing to disclose.

References

  1. Rädler, J.O.; Koltover, I.; Salditt, T.; Safinya, C.R. Structure of DNA–Cationic Liposome Complexes: DNA Intercalation in Multilamellar Membranes in Distinct Interhelical Packing Regimes. Science 1997, 275, 810–814. [Google Scholar] [CrossRef] [Green Version]
  2. Pozharski, E.; MacDonald, R.C. Lipoplex Thermodynamics: Determination of DNA-Cationic Lipoid Interaction Energies. Biophys. J. 2003, 85, 3969–3978. [Google Scholar] [CrossRef] [Green Version]
  3. Felgner, P.L.; Gadek, T.R.; Holm, M.; Roman, R.; Chan, H.W.; Wenz, M.; Northrop, J.P.; Ringold, G.M.; Danielsen, M. A highly efficient, lipid-mediated DNA-transfection procedure. Proc. Natl. Acad. Sci. USA 1987, 84, 7413–7417. [Google Scholar] [CrossRef] [Green Version]
  4. Mintzer, M.A.; Simanek, E.E. Nonviral Vectors for Gene Delivery. Chem. Rev. 2009, 109, 259–302. [Google Scholar] [CrossRef]
  5. Zhi, D.; Bai, Y.; Yang, J.; Cui, S.; Zhao, Y.; Chen, H.; Zhang, S. A review on cationic lipids with different linkers for gene delivery. Adv. Colloid Interface Sci. 2018, 253, 117–140. [Google Scholar] [CrossRef] [PubMed]
  6. Zhi, D.F.; Zhang, S.B.; Wang, B.; Zhao, Y.N.; Yang, B.L.; Yu, S.J. Transfection efficiency of cationic lipids with different hydrophobic domains in gene delivery. Bioconjugate Chem. 2010, 21, 563–577. [Google Scholar] [CrossRef]
  7. Bajaj, A.; Mishra, S.K.; Kondaiah, P.; Bhattachary, S. Effect of the headgroup variation on the gene transfer properties of cholesterol based cationic lipids possessing ether linkage. Biochim. Biophys. Acta 2008, 1778, 1222–1236. [Google Scholar] [CrossRef] [Green Version]
  8. Billiet, L.; Gonçalves, C.; Gomez, J.P.; Lodewick, J.; Berchel, M.; Jaffrès, P.A.; Montier, T.; Lehn, P.; Bertrand, E.; El-Ghoul, Y.; et al. Gene transfer by chemical vectors: Comparative study of the endocytosis routes of polyplexes, lipoplexes and lipopolyplexes in a myoblast cell line. Biomaterials 2012, 33, 2980–2990. [Google Scholar] [CrossRef]
  9. Singh, R.S.; Gonçalves, C.; Sandrin, P.; Pichon, C.; Midoux, P.; Chaudhuri, A. On the Gene Delivery Efficacies of pH-Sensitive Cationic Lipids via Endosomal Protonation: A Chemical Biology Investigation. Chem. Biol. 2004, 11, 713–723. [Google Scholar] [CrossRef]
  10. Mével, M.; Neveu, C.; Gonçalves, C.; Yaouanc, J.J.; Pichon, C.; Jaffrès, P.A.; Midoux, P. Novel neutral imidazole-lipophosphoramides for transfection assays. Chem. Commun. 2008, 3124–3126. [Google Scholar] [CrossRef] [Green Version]
  11. Fraix, A.; Le Gall, T.; Berchel, M.; Denis, C.; Lehn, P.; Montier, T.; Jaffrès, P.A. Cationic lipophosphoramidates with two disulfide motifs: Synthesis, behaviour in reductive media and gene transfection activity. Org. Biomol. Chem. 2013, 11, 1650–1658. [Google Scholar] [CrossRef] [PubMed]
  12. Puchkov, P.A.; Shmendel, E.V.; Luneva, A.S.; Zenkova, M.A.; Maslov, M.A. Position of Disulfide Bond Determines the Properties of Novel Stimuli-Responsive Cationic Lipids. Chem. Sel. 2020, 5, 4509–4514. [Google Scholar] [CrossRef]
  13. Reinhard, S.; Wang, Y.; Dengler, S.; Wagner, E. Precise Enzymatic Cleavage Sites for Improved Bioactivity of siRNA Lipo-Polyplexes. Bioconjugate Chem. 2018, 29, 3649–3657. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  14. Buck, J.; Grossen, P.; Cullis, P.R.; Huwyler, J.; Witzigmann, D. Lipid-Based DNA Therapeutics: Hallmarks of Non-Viral Gene Delivery. ACS Nano 2019, 13, 3754–3782. [Google Scholar] [CrossRef]
  15. Hou, X.; Zaks, T.; Langer, R.; Dong, Y. Lipid nanoparticles for mRNA delivery. Nat. Rev. Mater. 2021, 6, 1078–1094. [Google Scholar] [CrossRef]
  16. Pardi, N.; Hogan, M.J.; Porter, F.W.; Weissman, D. mRNA vaccines—A new aera in vaccinology. Nature Rev. Drug Discov. 2018, 17, 261–279. [Google Scholar] [CrossRef] [Green Version]
  17. Delalande, A.; Gosselin, M.P.; Suwalski, A.; Guilmain, W.; Leduc, C.; Berchel, M.; Jaffrès, P.A.; Baril, P.; Midoux, P.; Pichon, C. Enhanced Achilles tendon healing by fibromodulin gene transfer. Nanomed. NBM 2015, 11, 1735–1744. [Google Scholar] [CrossRef]
  18. Lindberg, M.F.; Le Gall, T.; Carmoy, N.; Berchel, M.; Hyde, S.C.; Gill, D.R.; Jaffrès, P.A.; Lehn, P.; Montier, T. Efficient in vivo transfection and safety profile of a CpG-free and codon optimized luciferaseplasmid using a cationic lipophosphoramidate in a multiple intravenous administration procedure. Biomaterials 2015, 59, 1–11. [Google Scholar] [CrossRef]
  19. Gaspar, D.P.; Vital, J.; Leiva, M.C.; Goncalves, L.M.D.; Taboada, P.; Remunan-Lopez, C.; Vitor, J.; Almeida, A.J. Transfection of pulmonary cells by stable pDNA-polycationic hybrid nanostructured particles. Nanomedicine 2019, 14, 407–429. [Google Scholar] [CrossRef] [PubMed]
  20. Künnapuu, K.; Veiman, K.L.; Porosk, L.; Rammul, E.; Kiisholts, K.; Langel, U.; Kurrikoff, K. Tumor gene therapy by systemic delivery of plasmid DNA with cell-penetrating peptides. FASEB BioAdv. 2019, 1, 105–114. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  21. Teo, P.Y.; Cheng, W.; Hedrick, J.L.; Yang, Y.Y. Co-deliveryofdrugsandplasmidDNAforcancertherapy. Adv. Drug Deliv. Rev. 2016, 98, 41–63. [Google Scholar] [CrossRef]
  22. Israelachvili, J.N.; Mitchell, D.J.; Ninham, B.W. Theory of self-assembly of hydrocarbon amphiphiles into micelles and bilayers. J. Chem. Soc. Faraday Trans. 1976, 72, 1525–1568. [Google Scholar] [CrossRef]
  23. Bouraoui, A.; Ghanem, R.; Berchel, M.; Deschamps, L.; Vié, V.; Paboeuf, G.; Le Gall, T.; Montier, T.; Jaffrès, P.A. Cationic amphiphiles producing hexagonal aggregates: Physico-chemical characterization and application to gene delivery. Org. Biomol. Chem. 2020, 18, 337–345. [Google Scholar] [CrossRef]
  24. Afonso, D.; Le Gall, T.; Couthon-Gourvès, H.; Grélard, A.; Prakash, S.; Berchel, M.; Kervarec, N.; Dufourc, E.J.; Montier, T.; Jaffrès, P.A. Thiol-ene click reaction applied to cationic amphiphiles: Impacts on supramolecular packing and gene transfection properties. Soft Matter 2016, 12, 4516–4520. [Google Scholar] [CrossRef] [PubMed]
  25. Fraix, A.; Montier, T.; Carmoy, N.; Loizeau, D.; Burel-Deschamps, L.; Le Gall, T.; Giamarchi, P.; Couthon-Gourvès, H.; Haelters, J.P.; Lehn, P.; et al. Cationic Lipothiophosphoramidates for in vitro gene delivery: Synthesis, physico-chemical characterizations and transfection assays- comparison with lipophosphoramidates. Org. Biomol. Chem. 2011, 9, 2422–2432. [Google Scholar] [CrossRef]
  26. Van der Jeught, K.; De Koker, S.; Bialkowski, L.; Heirman, C.; Tjok Joe, P.; Bevers, S.; Broos, K.; Deswarte, K.; Malard, V.; Hammad, H.; et al. Systemic delivery of modified mRNA through lipopolyplexes combines strong antitumor T cell immunity with improved inflammatory safety. ACS Nano 2018, 12, 9815–9829. [Google Scholar] [CrossRef]
  27. Dey, S.; Gupta, A.; Saha, A.; Pal, S.; Kumar, S.; Manna, D. Sunlight-Mediated Thiol−Ene/Yne Click Reaction: Synthesis and DNA Transfection Efficiency of New Cationic Lipids. ACS Omega 2020, 5, 735–750. [Google Scholar] [CrossRef]
  28. Naik, S.S.; Chan, J.W.; Comer, C.; Hoyle, C.E.; Savin, D.A. Thiol–yne ‘click’ chemistry as a route to functional lipid mimetics. Polym. Chem. 2011, 2, 303–305. [Google Scholar] [CrossRef]
  29. Saha, A.; Panda, S.; Pradhan, N.; Kalita, K.; Trivedi, V.; Manna, D. Azidophosphonate Chemistry as a Route for a Novel Class of Vesicle-Forming Phosphonolipids. Chem. Eur. J. 2018, 24, 1121–1127. [Google Scholar] [CrossRef]
  30. Bulpin, A.; Le Roy-Gourvennec, S.; Masson, S. The amination of phosphonodithioformates; a preparation of new functionalised phosphonates. Phosphorus Sulfur Sil. 1994, 89, 119–132. [Google Scholar] [CrossRef]
  31. Lebouc, F.; Dez, I.; Gulea, M.; Madec, P.J.; Jaffrès, P.A. Synthesis of phosphorus-containing chitosan derivatives. Phosphorus Sulfur Silicon Relat. Elem. 2009, 184, 872–889. [Google Scholar] [CrossRef]
  32. Khalil, M.; Jeanne Dit Fouque, D.; Berchel, M.; Fraix, A.; Dupont, A.; Sortino, S.; Memboeuf, A.; Jaffrès, P.A. Phosphonodithioester-Amine Coupling in water: A fast reaction to modify the surface of liposomes. Org. Biomol. Chem. 2021, 19, 6392–6396. [Google Scholar] [CrossRef]
  33. Pfund, E.; Lequeux, T.; Masson, S.; Vazeux, M.; Cordi, A.; Pierre, A.; Serred, V.; Herve, G. Efficient synthesis of fluorothiosparfosic acid analogues with potential antitumoral activity. Bioorg. Med. Chem. 2005, 13, 4921–4928. [Google Scholar] [CrossRef] [PubMed]
  34. Picquet, E.; Le Ny, K.; Delépine, P.; Montier, T.; Yaouanc, J.J.; Cartier, D.; des Abbayes, H.; Férec, C.; Clément, J.C. Cationic Lipophosphoramidates and Lipophosphoguanidines Are Very Efficient for in Vivo DNA Delivery. Bioconjugate Chem. 2005, 16, 1051–1053. [Google Scholar] [CrossRef]
  35. Le Corre, S.S.; Berchel, M.; Belmadi, N.; Denis, C.; Haelters, J.P.; Le Gall, T.; Lehn, P.; Montier, T.; Jaffrès, P.A. Cationic lipophosphoramidates with two different lipid chains: Synthesis and evaluation as gene carriers. Org. Biomol. Chem. 2014, 12, 1463–1474. [Google Scholar] [CrossRef]
  36. Bouraoui, A.; Berchel, M.; Ghanem, R.; Vié, V.; Paboeuf, G.; Deschamps, L.; Lozach, O.; Le Gall, T.; Montier, T.; Jaffrès, P.A. Thiol-ene click reaction to construct the lipid chains of cationic amphiphiles: Physico-chemical behaviour and application for gene delivery. Org. Biomol. Chem. 2019, 17, 3609–3616. [Google Scholar] [CrossRef]
  37. Le Corre, S.S.; Berchel, M.; Le Gall, T.; Haelters, J.P.; Lehn, P.; Montier, T.; Jaffrès, P.A. Cationic trialkylphosphates: Synthesis and transfection efficacies compared to phosphoramidate analogues. Eur. J. Org. Chem. 2014, 8041–8048. [Google Scholar] [CrossRef]
  38. Delbeke, E.I.P.; Lozach, O.; Le Gall, T.; Berchel, M.; Montier, T.; Jaffrès, P.A.; Van Geem, K.M.; Stevens, C.V. Evaluation of the transfection efficacies of quaternary ammonium salts prepared from sophorolipids. Org. Biomol. Chem. 2016, 14, 3744–3751. [Google Scholar] [CrossRef] [PubMed]
  39. Le Gall, T.; Loizeau, D.; Picquet, E.; Carmoy, N.; Yaouanc, J.J.; Burel-Deschamps, L.; Delépine, P.; Giamarchi, P.; Jaffès, P.A.; Lehn, P.; et al. A Novel Cationic Lipophosphoramide with Diunsaturated Lipid Chains: Synthesis, Physicochemical Properties, and Transfection Activities. J. Med. Chem. 2010, 53, 1496–1508. [Google Scholar] [CrossRef] [PubMed]
  40. Grisley, D.W. The reaction of sodium dialkyl phosphonates with carbonyl sulfide and with carbon disulfide. J. Org. Chem. 1961, 26, 2544–2546. [Google Scholar] [CrossRef]
  41. Heuzé, B.; Lemarié, M.; Vazeux, M.; Gulea, M.; Masson, S.; Sene, A.; Jaffrès, P.A.; Alberti, A.; Macciantelli, D. Synthesis of methylene diphosphonates from carbon disulfide and phosphites via desulfurization: A mechanistic study. Phosphorus Sulfur Silicon Relat. Elem. 2009, 184, 820–829. [Google Scholar] [CrossRef]
  42. Hyde, S.C.; Pringle, I.A.; Abdullah, S.; Lawton, A.E.; Davies, L.A.; Varathalingam, A.; Nunez-Alonso, G.; Green, A.M.; Bazzani, R.P.; Sumner-Jones, S.G.; et al. CpG-free plasmids confer reduced inflammation and sustained pulmonary gene expression. Nat. Biotechnol. 2008, 26, 549–551. [Google Scholar] [CrossRef] [PubMed]
  43. Laurent, V.; Fraix, A.; Montier, T.; Cammas-Marion, S.; Ribault, C.; Benvegnu, T.; Jaffrès, P.A.; Loyer, P. Highly efficient gene transfer into hepatocyte-like HepaRG cells: New means for drug metabolism and toxicity studies. Biotechnol. J. 2010, 5, 314–320. [Google Scholar] [CrossRef] [Green Version]
  44. Sharma, D.; Arora, S.; Singh, J.; Layek, B. A review of the tortuous path of nonviral gene delivery and recent progress. Inter. J. Biol. Macromol. 2021, 183, 2055–2073. [Google Scholar] [CrossRef]
Molecules 26 07507 g001 550
Figure 1. Chemical structure of the two new cationic amphiphiles 4a–b reported in this work and the structure of KLN47 and BSV36, which were used as references for the transfection experiments.
Figure 1. Chemical structure of the two new cationic amphiphiles 4a–b reported in this work and the structure of KLN47 and BSV36, which were used as references for the transfection experiments.
Molecules 26 07507 g001
Molecules 26 07507 g002 550
Figure 2. Synthesis of cationic amphiphiles. (i) Heating at 140 °C under vacuum in a Kugelrohr distillation apparatus for 4 h; (ii) NaH, anhydrous THF, reflux, 4 h; (iii) addition of CS2 at −78 °C, then warmed up and stirred at RT for 2 h; (iv) CH3I; (v) N,N-dimethylethylenediamine; (vi) CH3I, RT, 2 h.
Figure 2. Synthesis of cationic amphiphiles. (i) Heating at 140 °C under vacuum in a Kugelrohr distillation apparatus for 4 h; (ii) NaH, anhydrous THF, reflux, 4 h; (iii) addition of CS2 at −78 °C, then warmed up and stirred at RT for 2 h; (iv) CH3I; (v) N,N-dimethylethylenediamine; (vi) CH3I, RT, 2 h.
Molecules 26 07507 g002
Molecules 26 07507 g003 550
Figure 3. Gel electrophoresis of complexes prepared by mixing LF1-3, BSV36, KLN47 and pGM144 at CRs ranging from 0.7 to 4 in water.
Figure 3. Gel electrophoresis of complexes prepared by mixing LF1-3, BSV36, KLN47 and pGM144 at CRs ranging from 0.7 to 4 in water.
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Molecules 26 07507 g004 550
Figure 4. In vitro transfection efficacies (RLU/mg of protein) as a function of the charge ratios (CR from 0.7 to 4) in four cell lines: (A) A549; (B) Calu-3; (C) 16HBE; (D) CFBE.
Figure 4. In vitro transfection efficacies (RLU/mg of protein) as a function of the charge ratios (CR from 0.7 to 4) in four cell lines: (A) A549; (B) Calu-3; (C) 16HBE; (D) CFBE.
Molecules 26 07507 g004
Molecules 26 07507 g005 550
Figure 5. Cell viability as a function of the charge ratio (CR = 0.7 to 4). (A) A549; (B) Calu-3; (C) 16HBE; (D) CFBE.
Figure 5. Cell viability as a function of the charge ratio (CR = 0.7 to 4). (A) A549; (B) Calu-3; (C) 16HBE; (D) CFBE.
Molecules 26 07507 g005
Table
Table 1. Composition and method used to prepare the formulations LF1–3 and the reference formulations BSV36 and KLN47.
Table 1. Composition and method used to prepare the formulations LF1–3 and the reference formulations BSV36 and KLN47.
FormulationsCompositionRatioConcentrationMethod
BSV36BSV3611.5 mMLipid film hydration
KLN47KLN4711.5 mMLipid film hydration
LF14a: DOPE1: 11.5 mM in 4aLipid film hydration
LF24a: Chol: DOPE1: 1: 11.5 mM in 4aLipid film hydration
LF34b: DOPE1: 11.5 mM in 4bEthanol injection
Table
Table 2. Sizes and zeta potentials of liposomal solutions.
Table 2. Sizes and zeta potentials of liposomal solutions.
FormulationsSize (nm)PDIPotential Zeta (mV)
BSV36169 ± 90.2458 ± 0.06
KLN47126 ± 70.3328 ± 1.16
LF1227 ± 410.4661 ± 1.7
LF2194 ± 160.4479 ± 0.6
LF3229 ± 150.3357 ± 0.8
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Khalil, M.; Hocquigny, A.; Berchel, M.; Montier, T.; Jaffrès, P.-A. Phosphonodithioester–Amine Coupling as a Key Reaction Step for the Design of Cationic Amphiphiles Used for Gene Delivery. Molecules 2021, 26, 7507. https://doi.org/10.3390/molecules26247507

AMA Style

Khalil M, Hocquigny A, Berchel M, Montier T, Jaffrès P-A. Phosphonodithioester–Amine Coupling as a Key Reaction Step for the Design of Cationic Amphiphiles Used for Gene Delivery. Molecules. 2021; 26(24):7507. https://doi.org/10.3390/molecules26247507

Chicago/Turabian Style

Khalil, Montassar, Alexis Hocquigny, Mathieu Berchel, Tristan Montier, and Paul-Alain Jaffrès. 2021. "Phosphonodithioester–Amine Coupling as a Key Reaction Step for the Design of Cationic Amphiphiles Used for Gene Delivery" Molecules 26, no. 24: 7507. https://doi.org/10.3390/molecules26247507

APA Style

Khalil, M., Hocquigny, A., Berchel, M., Montier, T., & Jaffrès, P. -A. (2021). Phosphonodithioester–Amine Coupling as a Key Reaction Step for the Design of Cationic Amphiphiles Used for Gene Delivery. Molecules, 26(24), 7507. https://doi.org/10.3390/molecules26247507

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