Antioxidant and Antisteatotic Activities of a New Fucoidan Extracted from Ferula hermonis Roots Harvested on Lebanese Mountains
Abstract
:1. Introduction
2. Results
2.1. Extraction Yield and Chemical Composition
2.2. Spectroscopic Characterization
2.3. Radical Scavenging Capacity
2.4. Antioxidant and Antisteatotic Activities of FUFe in a Cellular Model of Hepatic Steatosis
2.5. Effects of FUFe on Steatotic Endothelial Cells
3. Discussion
4. Materials and Methods
4.1. Biological Material and Chemicals
4.2. Extraction and Purification of Fucoidan
4.3. Chemical Characterization
4.4. Radical Scavenging Capacity
4.4.1. DPPH (2,2-diphenyl-1-picrylhydrazyl radical) Assay
4.4.2. ABTS (2,2-azinobis-3-ethylbenzothiazoline-6-sulfonic acid) Assay
4.4.3. FRAP (Ferric Reducing Antioxidant Power) Assay
4.5. Cell Culture and Treatments
4.6. ROS Production
4.7. Quantification of Triglycerides (TGs)
4.8. Nitric Oxide (NO) Production
4.9. RNA Extraction and Quantitative Real-Time PCR
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Sulfate | Fucose | Glucose | Galactose | Mannose | Proteins |
---|---|---|---|---|---|
1.8 ± 1.52 | 31.5 ± 6.1 | 29.3 ± 6.32 | 2.6 ± 5.82 | 1.2 ± 4.95 | 0.46 ± 7.6 |
FUFe (µg/mL) | RSA% |
---|---|
50 | 18.1 ± 4.2 |
75 | 26.6 ± 9.2 |
100 | 36.4 ± 9.1 |
200 | 60.5 ± 11.2 |
300 | 72.8 ± 5.1 |
400 | 75.7 ± 4.9 |
500 | 75.7 ± 4.2 |
IC50 = 157.6 ± 3.3 µg/mL |
ABTS | FRAP | ||
---|---|---|---|
TEAC (μmol TE/g) | IC50 (μg/mL) | TEAC (μmol TE/g) | |
Fucoidan from F. hermonis (FUFe) | 410.0 ± 14.0 | 44.26 ± 2.35 | 81.30 ± 7.20 |
Trolox | 4.53 ± 1.53 |
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El Rashed, Z.; Lupidi, G.; Kanaan, H.; Grasselli, E.; Canesi, L.; Khalifeh, H.; Demori, I. Antioxidant and Antisteatotic Activities of a New Fucoidan Extracted from Ferula hermonis Roots Harvested on Lebanese Mountains. Molecules 2021, 26, 1161. https://doi.org/10.3390/molecules26041161
El Rashed Z, Lupidi G, Kanaan H, Grasselli E, Canesi L, Khalifeh H, Demori I. Antioxidant and Antisteatotic Activities of a New Fucoidan Extracted from Ferula hermonis Roots Harvested on Lebanese Mountains. Molecules. 2021; 26(4):1161. https://doi.org/10.3390/molecules26041161
Chicago/Turabian StyleEl Rashed, Zeinab, Giulio Lupidi, Hussein Kanaan, Elena Grasselli, Laura Canesi, Hala Khalifeh, and Ilaria Demori. 2021. "Antioxidant and Antisteatotic Activities of a New Fucoidan Extracted from Ferula hermonis Roots Harvested on Lebanese Mountains" Molecules 26, no. 4: 1161. https://doi.org/10.3390/molecules26041161