Radiofrequency Irradiation Attenuates High-Mobility Group Box 1 and Toll-like Receptor Activation in Ultraviolet B–Induced Skin Inflammation
Abstract
:1. Introduction
2. Results
2.1. Inhibitory Effect of RF on Inflammatory Cytokine Secreted by UVB-Effected Keratinocytes
2.2. Inhibition Effect on Macrophage Activation by RF-Irradiated Keratinocytes
2.3. Inhibitory Effects on the TLR Pathway of Macrophages Affected by RF-Irradiated Keratinocytes
2.4. The Inhibitory Effects of RF Irradiation on Macrophages Affect Keratinocyte Proliferation and Pigment Accumulation
3. Discussion
4. Materials and Methods
4.1. UVB-Induced Skin Inflammation Models
4.1.1. Animal Model (In Vivo)
4.1.2. Cell Model (In Vitro)
4.2. RF Irradiation Conditions for Patients
4.3. Devices for RF Irradiation
4.4. Cell Proliferation Measurement
4.5. Sample Preparation
4.5.1. Paraffin-Embedded Tissue Section Processing
4.5.2. RNA Extraction and cDNA Synthesis
4.6. Quantitative Real-Time Polymerase Chain Reaction
4.7. Immunostaining Using 3,3-Diaminobenzidine
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Kim, H.M.; Oh, S.; Yoon, J.H.; Kang, D.; Son, M.; Byun, K. Radiofrequency Irradiation Attenuates High-Mobility Group Box 1 and Toll-like Receptor Activation in Ultraviolet B–Induced Skin Inflammation. Molecules 2021, 26, 1297. https://doi.org/10.3390/molecules26051297
Kim HM, Oh S, Yoon JH, Kang D, Son M, Byun K. Radiofrequency Irradiation Attenuates High-Mobility Group Box 1 and Toll-like Receptor Activation in Ultraviolet B–Induced Skin Inflammation. Molecules. 2021; 26(5):1297. https://doi.org/10.3390/molecules26051297
Chicago/Turabian StyleKim, Hyoung Moon, Seyeon Oh, Jung Hyun Yoon, Donghwan Kang, Myeongjoo Son, and Kyunghee Byun. 2021. "Radiofrequency Irradiation Attenuates High-Mobility Group Box 1 and Toll-like Receptor Activation in Ultraviolet B–Induced Skin Inflammation" Molecules 26, no. 5: 1297. https://doi.org/10.3390/molecules26051297