Polydopamine-Assisted Rapid One-Step Immobilization of L-Arginine in Capillary as Immobilized Chiral Ligands for Enantioseparation of Dansyl Amino Acids by Chiral Ligand Exchange Capillary Electrochromatography
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of L-Arg/PDA Coating
2.1.1. Field Emission Scanning Electron Microscopy (FESEM)
2.1.2. Fourier-Transform Infrared Spectroscopy (FTIR) and Attenuated Total Reflectance FTIR (ATR-FTIR)
2.1.3. Electroosmotic Flow (EOF)
2.2. Optimization of PDA/L-Arg@Capillary Preparation
2.2.1. Effects of the Molar Ratio of Dopamine and L-Arg on Enantioseparation Capacity
2.2.2. Influences of Hydrothermal Temperature and Time of PDA/L-Arg Layer on Enantioseparation Capacity
2.3. CLE-CEC Operation Conditions Optimization
2.4. Enantioseparation Performance of PDA/L-Arg@Capillary
2.5. Repeatability and Stability of PDA/L-Arg@Capillary
2.6. Exploration of Enantioseparation Scheme in Presented CLE-CEC
2.7. Quantitative Determination of D,L-Glu
2.8. Enzyme Kinetic Study of L-GLDH
3. Materials and Methods
3.1. Materials and Chemicals
3.2. Apparatus
3.3. Buffer Solution and Sample Solution Preparation
3.4. CLE-CEC Procedures
3.5. Fabrication of PDA/L-Arg@Capillary
3.6. Enzyme Kinetics Study of L-GLDH
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gui, Y.; Ji, B.; Yi, G.; Li, X.; Zhang, K.; Fu, Q. Polydopamine-Assisted Rapid One-Step Immobilization of L-Arginine in Capillary as Immobilized Chiral Ligands for Enantioseparation of Dansyl Amino Acids by Chiral Ligand Exchange Capillary Electrochromatography. Molecules 2021, 26, 1800. https://doi.org/10.3390/molecules26061800
Gui Y, Ji B, Yi G, Li X, Zhang K, Fu Q. Polydopamine-Assisted Rapid One-Step Immobilization of L-Arginine in Capillary as Immobilized Chiral Ligands for Enantioseparation of Dansyl Amino Acids by Chiral Ligand Exchange Capillary Electrochromatography. Molecules. 2021; 26(6):1800. https://doi.org/10.3390/molecules26061800
Chicago/Turabian StyleGui, Yuanqi, Baian Ji, Gaoyi Yi, Xiuju Li, Kailian Zhang, and Qifeng Fu. 2021. "Polydopamine-Assisted Rapid One-Step Immobilization of L-Arginine in Capillary as Immobilized Chiral Ligands for Enantioseparation of Dansyl Amino Acids by Chiral Ligand Exchange Capillary Electrochromatography" Molecules 26, no. 6: 1800. https://doi.org/10.3390/molecules26061800