Neuroprotective and Antioxidant Enhancing Properties of Selective Equisetum Extracts
Abstract
:1. Introduction
2. Results and Discussion
2.1. Obtaining Extracts
2.2. Semi-Quantitative and Qualitative Determination of the Polyphenolic Compounds (UHPLC-DAD)
2.3. In Vitro Evaluation of the Antioxidant Activity of the Plant Extracts Obtained from the Three Species of the Equisetum Genus
2.3.1. The Chelating Capacity of the Ferrous Ion Determination
2.3.2. Determination of the Lipoxygenase Inhibition Capacity
2.3.3. Determination of the Scavenger Action of the Hydroxyl Radical
2.3.4. Determination of the Scavenger Capacity of the Superoxide Anion
2.4. In Vivo Evaluation of the Neuroprotective Activity of the Plant Extracts Obtained from the Three Species of Equisetum
2.4.1. Novel Tank Diving Test (NTT)
2.4.2. Y-Maze Test
3. Materials and Methods
3.1. Plant Materials
3.2. UHPLC Analysis of Phenolic Acids and Flavonoids
3.3. Antioxidant Capacity Assay
3.4. Animals and Group Division
3.5. Behavioral Assays
3.5.1. Novel Tank Diving Test (NTT)
3.5.2. Y-Maze Test
3.6. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Compound Expressed in mg/g Plant Product * | Sample (70% Methanolic Extract) | Sample (70% Ethanolic Extract) | ||||
---|---|---|---|---|---|---|
E. sylvaticum | E. pratense | E. telmateia | E. sylvaticum | E. pratense | E. telmateia | |
epicatechin | 0.9010 ± 0.021 | 0.5925 ± 0.001 | 1.1967 ± 0.001 | 0.1586 ± 0.002 | 0.0263 ± 0.003 | 0.7079 ± 0.011 |
quercetin-3-glucoside | 1.6995 ± 0.001 | 8.2442 ± 0.001 | 23.4765 ± 0.033 | 41.9429 ± 0.022 | 3.8440 ± 0.001 | 13.6175 ± 0.022 |
luteolin-7-glucoside | 11.0456 ± 0.011 | 1.8562 ± 0.001 | 5.6447 ± 0.021 | 3.7656 ± 0.019 | 0.0378 ± 0.011 | 1.2254 ± 0.003 |
apigenin-7-glucoside | 2.3249 ± 0.013 | 2.2947 ± 0.021 | 27.7463 ± 0.009 | 7.8806 ± 0.003 | 1.4626 ± 0.004 | 21.0042 ± 0.021 |
luteolin | 0.6150 ± 0.011 | 0.0344 ± 0.003 | 0.4214 ± 0.009 | 0.4069 ± 0.011 | 0.0076 ± 0.021 | 0.1455 ± 0.011 |
quercetin | 0.0658 ± 0.001 | 0.0059 ± 0.002 | 0.4013 ± 0.022 | 0.0255 ± 0.009 | 0.0071 ± 0.001 | 0.1369 ± 0.005 |
apigenin | 0.0716 ± 0.002 | 0.6260 ± 0.009 | 0.9571 ± 0.009 | 0.0591 ± 0.001 | 0.1359 ± 0.002 | 0.3758 ± 0.001 |
kaempferol | 1.4997 ± 0.021 | 0.9781 ± 0.009 | 1.4417 ± 0.009 | 0.0216 ± 0.009 | 0.2022 ± 0.001 | 0.7609 ± 0.004 |
Compound Expressed in mg/g Plant Product * | Sample (70% Methanolic Extract) | Sample (70% Ethanolic Extract) | ||||
---|---|---|---|---|---|---|
E. sylvaticum | E. pratense | E. telmateia | E. sylvaticum | E. pratense | E. telmateia | |
neochlorogenic acid | 0.2639 ± 0.001 | 0.0730 ± 0.001 | 0.6381 ± 0.005 | 0.4838 ± 0.002 | 0.1879 ± 0.002 | 0.3059 ± 0.002 |
chlorogenic acid | 4.3322 ± 0.001 | 0.7491 ± 0.002 | 10.1162 ± 0.002 | 3.6744 ± 0.001 | 0.7362 ± 0.003 | 14.3814 ± 0.004 |
caffeic acid | 0.1468 ± 0.002 | 0.2363 ± 0.002 | 0.8590 ± 0.003 | 0.6624 ± 0.002 | 0.1922 ± 0.001 | 0.3244 ± 0.002 |
ferulic acid | 0.6414 ± 0.002 | 0.2295 ± 0.005 | 1.0245 ± 0.001 | 5.7962 ± 0.004 | 0.5559 ± 0.001 | 1.0439 ± 0.003 |
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Batir-Marin, D.; Boev, M.; Cioanca, O.; Mircea, C.; Burlec, A.F.; Beppe, G.J.; Spac, A.; Corciova, A.; Hritcu, L.; Hancianu, M. Neuroprotective and Antioxidant Enhancing Properties of Selective Equisetum Extracts. Molecules 2021, 26, 2565. https://doi.org/10.3390/molecules26092565
Batir-Marin D, Boev M, Cioanca O, Mircea C, Burlec AF, Beppe GJ, Spac A, Corciova A, Hritcu L, Hancianu M. Neuroprotective and Antioxidant Enhancing Properties of Selective Equisetum Extracts. Molecules. 2021; 26(9):2565. https://doi.org/10.3390/molecules26092565
Chicago/Turabian StyleBatir-Marin, Denisa, Monica Boev, Oana Cioanca, Cornelia Mircea, Ana Flavia Burlec, Galba Jean Beppe, Adrian Spac, Andreia Corciova, Lucian Hritcu, and Monica Hancianu. 2021. "Neuroprotective and Antioxidant Enhancing Properties of Selective Equisetum Extracts" Molecules 26, no. 9: 2565. https://doi.org/10.3390/molecules26092565