Berberrubine Phosphate: A Selective Fluorescent Probe for Quadruplex DNA
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis
2.2. Absorption and Emission Properties
2.3. Photometric DNA Titrations
2.4. Thermal DNA Denaturation Experiments
2.5. CD and LD Spectroscopy
2.6. Fluorimetric Titrations
3. Materials and Methods
3.1. Equipment
3.2. Materials
3.3. Synthesis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Solvent | λabsa/nm | lg ε b | λflc/nm | νabs − νfl d/cm−1 | Φfle | ||||
---|---|---|---|---|---|---|---|---|---|
2 | 1a f | 2 | 2 | 1a f | 2 | 1a f | 2 | 1a f | |
MeOH | 431 | 429 | 3.98 | 558 | 540 | 5280 | 4790 | 0.01 | 0.01 |
EtOH | 431 | 430 | 3.98 | 537 | 536 | 4580 | 4600 | 0.02 | 0.03 |
1-PrOH | 431 | 3.94 | 531 | 4369 | 0.04 | ||||
MeCN | 431 | 430 | 3.91 | 537 | 537 | 4579 | 4630 | 0.03 | 0.02 |
DMSO | 430 | 425 | 3.97 | 547 | 553 | 4974 | 5500 | 0.01 | <0.01 |
Buffer | 424 | 3.92 | 535 | 4893 | <0.01 |
DNA | Kb/105 M−1 b | Φfl c | ∆Tm/°C d |
---|---|---|---|
ct DNA | 2.2 ± 0.2 | <0.01 | |
22AG/F21Ta | 5.0 ± 0.6 | 0.01 | 3.6 |
c-kit/FkitTa | 6.9 ± 0.2 | 0.01 | 1.0 |
a2/Fa2Ta | 6.7 ± 0.2 | 0.01 | 0.6 |
c-myc/FmycT a | 5.9 ± 0.4 | 0.01 | 2.2 |
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Wickhorst, P.J.; Ihmels, H. Berberrubine Phosphate: A Selective Fluorescent Probe for Quadruplex DNA. Molecules 2021, 26, 2566. https://doi.org/10.3390/molecules26092566
Wickhorst PJ, Ihmels H. Berberrubine Phosphate: A Selective Fluorescent Probe for Quadruplex DNA. Molecules. 2021; 26(9):2566. https://doi.org/10.3390/molecules26092566
Chicago/Turabian StyleWickhorst, Peter Jonas, and Heiko Ihmels. 2021. "Berberrubine Phosphate: A Selective Fluorescent Probe for Quadruplex DNA" Molecules 26, no. 9: 2566. https://doi.org/10.3390/molecules26092566