Dual-Template Magnetic Molecularly Imprinted Polymer for Simultaneous Determination of Spot Urine Metanephrines and 3-Methoxytyramine for the Diagnosis of Pheochromocytomas and Paragangliomas
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Material
2.2. Patients
2.3. Synthesis of MMIP and Magnetic Non-Imprinted Polymer
2.4. Performance of d-SPE
2.5. Chromatographic Conditions
3. Results and Discussion
3.1. MMIP Synthesis
3.2. Optimization of MMIP Synthesis
3.3. The Characteristics of MMIP
3.4. The Optimization of d-SPE Conditions
3.5. Precision, Usage Count and Stability of MMIP
3.6. Recoveries and Calibration Curves
3.7. The Methods Comparison between SPE and d-SPE
3.8. The Diagnosis of PPGLs
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Template | EA (%) of Standards | ||
---|---|---|---|
NMN | MN | 3-MT | |
DA | 94.5 ± 0.0 | 90.8 ± 0.7 | 94.9 ± 0.1 |
E | 78.1 ± 1.3 | 73.4 ± 0.6 | 82.0 ± 0.6 |
NE | 84.6 ± 0.3 | 75.8 ± 0.9 | 87.3 ± 0.6 |
NMN | 52.5 ± 2.2 | 46.6 ± 0.3 | 61.5 ± 2.3 |
MN | 57.8 ± 1.1 | 64.3 ± 1.2 | 48.0 ± 0.6 |
3-MT | 56.4 ± 0.9 | 32.7 ± 1.4 | 45.7 ± 2.2 |
DA +MN | 91.3 ± 0.1 | 89.4 ± 0.2 | 94.6 ± 0.0 |
MNIP | 8.9 ± 1.2 | 9.4 ± 0.5 | 8.5 ± 0.5 |
Template | Peak Area | ||
---|---|---|---|
NMN | MN | 3-MT | |
DA | 287.5 ± 8.3 | 2.1 ± 0.1 | 44.1 ± 1.6 |
E | 12.2 ± 0.5 | 12.0 ± 0.4 | 10.1 ± 0.1 |
NE | 3.8 ± 0.3 | 3.6 ± 0.3 | 82.9 ± 7.3 |
NMN | 26.3 ± 0.5 | 0.0 | 5.0 ± 0.5 |
MN | 5.6 ± 0.2 | 42.9 ± 0.3 | 49.0 ± 0.8 |
3-MT | 24.6 ± 0.1 | 29.3 ± 0.8 | 51.1 ± 0.7 |
DA + MN | 25.8 ± 1.0 | 40.3 ± 1.2 | 37.4 ± 1.8 |
MNIP | 3.2 ± 0.1 | 5.6 ± 0.1 | 3.0 ± 1.6 |
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Zeng, H.; Zhang, X.; Zhen, Q.; He, Y.; Wang, H.; Zhu, Y.; Sun, Q.; Ding, M. Dual-Template Magnetic Molecularly Imprinted Polymer for Simultaneous Determination of Spot Urine Metanephrines and 3-Methoxytyramine for the Diagnosis of Pheochromocytomas and Paragangliomas. Molecules 2022, 27, 3520. https://doi.org/10.3390/molecules27113520
Zeng H, Zhang X, Zhen Q, He Y, Wang H, Zhu Y, Sun Q, Ding M. Dual-Template Magnetic Molecularly Imprinted Polymer for Simultaneous Determination of Spot Urine Metanephrines and 3-Methoxytyramine for the Diagnosis of Pheochromocytomas and Paragangliomas. Molecules. 2022; 27(11):3520. https://doi.org/10.3390/molecules27113520
Chicago/Turabian StyleZeng, Hongyu, Xiaoqing Zhang, Qianna Zhen, Yifan He, Haoran Wang, Yang Zhu, Qi Sun, and Min Ding. 2022. "Dual-Template Magnetic Molecularly Imprinted Polymer for Simultaneous Determination of Spot Urine Metanephrines and 3-Methoxytyramine for the Diagnosis of Pheochromocytomas and Paragangliomas" Molecules 27, no. 11: 3520. https://doi.org/10.3390/molecules27113520