Next Article in Journal
Synergistic Treatment of Congo Red Dye with Heat Treated Low Rank Coal and Micro-Nano Bubbles
Next Article in Special Issue
Mulberry Ethanol Extract and Rutin Protect Alcohol-Damaged GES-1 Cells by Inhibiting the MAPK Pathway
Previous Article in Journal
Characterization of Roasting Time on Sensory Quality, Color, Taste, and Nonvolatile Compounds of Yuan An Yellow Tea
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates

1
Department of Agro-Food Resources, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Korea
2
Department of Food Science and Technology, Jeonbuk National University, Jeonju 54896, Korea
3
National Agrobiodiversity Center, National Institute of Agricultural Sciences, Rural Development Administration, Jeonju 54874, Korea
*
Authors to whom correspondence should be addressed.
Molecules 2022, 27(13), 4120; https://doi.org/10.3390/molecules27134120
Submission received: 13 June 2022 / Revised: 23 June 2022 / Accepted: 24 June 2022 / Published: 27 June 2022
(This article belongs to the Special Issue Bioactive Compounds in Fermented Foods and Beverages)

Abstract

:
In this study, thirty-eight isoflavone derivatives were comprehensively identified and quantified from the raw, steamed and fermented seeds of four selected soybean cultivars based on UPLC-DAD-QToF/MS results with reference to the previously reported LC-MS library and flavonoid database, and summarized by acylated group including glucosides (Glu), malonyl-glucosides (Mal-Glu), acetyl-glucosides (Ac-Glu), succinyl-glucosides (Suc-Glu) and phosphorylated conjugates (Phos) in addition to aglycones. Among them, Suc-Glu and Phos derivatives were newly generated due to fermentation by B. subtilis AFY-2 (cheonggukjang). In particular, Phos were characterized for the first time in fermented soy products using Bacillus species. From a proposed roadmap on isoflavone-based biotransformation, predominant Mal-Glu (77.5–84.2%, raw) decreased rapidly by decarboxylation and deesterification into Ac-Glu and Glu (3.5–8.1% and 50.0–72.2%) during steaming, respectively. As fermentation continued, the increased Glu were mainly succinylated and phosphorylated as well as gradually hydrolyzed into their corresponding aglycones. Thus, Suc-Glu and Phos (17.3–22.4% and 1.5–5.4%, 36 h) determined depending on cultivar type and incubation time, and can be considered as important biomarkers generated during cheonggukjang fermentation. Additionally, the changes of isoflavone profile can be used as a fundamental report in applied microbial science as well as bioavailability research from fermented soy foods.

1. Introduction

Isoflavones are mainly distributed as O-glycosyl or C-glycosyl derivatives with acylated moieties (e.g., none-, acetyl- and malonyl-) in soybean, kudzu, astragalus and red clover belonging to Leguminosae Family [1,2] and have been reported to have potential preventive effects in a wide range of human diseases such as cancer, hypertension, atherosclerosis, diabetes, hyperlipidemia, menopausal symptoms, osteoporosis, neurodegeneration and multiple sclerosis [1,3,4]. Particularly, soybean (Glycine max L.), commonly known as an isoflavone-rich source, is one of the most important crops consumed worldwide because it provides the functional nutrients (vegetable protein, oil, fatty acid, isoflavone, saponin, etc.) and related health benefits through dietary soy-based foods (e.g., bean sprouts, soymilk, soy paste, tofu, yuba, miso, natto, doenjang and cheonggukjang) [5,6,7]. In general, cheonggukjang and natto, which are representatively traditional fermented foods in Korea and Japan, respectively, are produced under different Bacillus species (B. subtilis, cheonggukjang; B. subtilis natto, natto) and processed conditions during fermentation [8,9].
According to previous reports, malonyl-glucosides predominantly found in raw soybeans can be decarboxylated and deesterified into acetyl-glucosides and glucosides, respectively, by thermal procedures including steaming, baking and roasting [10,11]. Additionally, as fermentation continued, the increased glucosides after steaming were hydrolyzed to the corresponding aglycones by certain Bacillus species with high β-glucosidase activity, and followed by biosynthesized into new derivatives of succinyl-glucosides and phosphorylated conjugates [12,13,14]. Considering these microbial biotransformations, most isoflavone studies during cheonggukjang and natto fermentations have been focused on the changes in their profiles under affected by type and incubation time of microorganisms as well as cooking temperature [13,15,16,17].
It has been reported that the malonyl-glucosides decreased, while the glucosides increased depending on heat-treated temperature and time during steaming and roasting, which is required in soybean cooking [7,11,18,19]. Moreover, when cheonggukjang and natto were fermented, the enzymatically 6″-O-acylated glycosides were newly identified as 6″-O-succinyldaidzin, 6″-O-succinylgenistin and 6″-O-succinylglycitin, accounting for 4.8, 7.2 and 0.6% of the total isoflavones (TIFs, natto), respectively, based on mass (MS) and nuclear magnetic resonance (NMR) spectroscopies, and found to be derivatives to play a preventive role on bone loss of an ovarian hormone-deficiency rat model [8,9]. Among them, 6″-O-succinyldaidzin of Japanese natto elicited anti-ischemic effects by reducing both infarct areas and neurological scores of the middle cerebral artery occlusion rats [20]. The Korean cheonggukjang presented significantly clinical effects in hyperlipidemia-induced hamsters [21], and 6″-O-succinyldaidzin and 6″-O-succinylgenistin derived from these foods were rapidly accumulated after 15–18 h prior to maximum conversion after 27–36 h during their fermentation [22,23].
Recently, Kwon et al. [24] identified and quantified a total of eight succinylated derivatives (20.3% and 13.6% of TIFs), including additional 4″-O-succinyldaidzin and 4″-O-succinylgenistin from commercially available cheonggukjang and natto using high-resolution QToF-MS coupled with UPLC-DAD system. As phosphorylated isoflavones, four conjugates were newly generated via biotransformation on the growth of B. subtilis and B. amyloliquefaciens cultivated with pure standards (daidzein, genistein, daidzin and genistin), and determined as 7-O-phosphate or 4′-O-phosphate based on daidzein and genistein by MS fragmentation and NMR elucidation [14,25]. However, these conjugates have not been directly characterized from the fermented soy foods. Other isomers associated with 6″-O-succinyl-glucosides are still limited in their detailed changes with different fermented conditions. Since the simplified isoflavone structures caused by fermentation lead to faster absorption and higher bioavailability after consumption of cheonggukjang in human clinical studies [26], it is also necessary to provide a comprehensive profile in the changed isoflavone derivatives.
Thus, in this study, a comprehensive isoflavones profiling was precisely performed through spectral interpretation of QToF-MS with reference to previously reported LC-MS library and flavonoid database (RDA DB 1.0 published in 2016) from the raw, steamed and fermented soybean seeds. Additionally, it was purposed to evaluate the isoflavone changes including primarily succinylated and phosphorylated conjugates during steaming and cheonggukjang fermentation (by B. subtilis AFY-2) with four selected cultivars based on UPLC-DAD. In order to regulate key metabolites during steaming and fermentation, a roadmap on biotransformation of soy isoflavones could also be proposed consisting of these completed profiles. Finally, the profile of isoflavone changes provided in the present study suggest that the presence of succinylated and phosphorylated isoflavones can be considered as important biomarkers during fermentation with Bacillus species, and can be used as a fundamental report in advanced microbial science as well as human bioavailability research after consumption of fermented soy foods.

2. Results and Discussion

2.1. Identification of 38 Isoflavone Derivatives in Raw, Steamed and Fermented Soybean Seeds

As shown in Figure 1 and Table 1, a total of thirty-eight isoflavone derivatives were tentatively identified from the raw, steamed and fermented seed samples of four selected soybean cultivars (KLS 87248, Nongrim 51, GNU-2007-14613 and Daewon) by comparing elution order, UV spectra and MS fragments pattern provided from the present UPLC-DAD and QToF-MS spectral interpretation as well as previously reported LC-MS library and NMR-elucidated results, and mainly composed of glucosides (four), malonyl-glucosides (nine), acetyl-glucosides (seven), succinyl-glucosides (nine) and phosphorylated conjugates (four) based on daidzein, genistein and glycitein aglycones. These derivatives with good separation on UPLC-DAD chromatograms (KLS 87248, wavelength at 254 nm) (Figure 1) were fragmented in detail through positive ionization (+ESI, m/z [M + H]+) of high-resolution QToF-MS (Table 1).
Through recent high-resolution MS technologies, the positive ionization makes it easier to distinguish the parent molecules than conventional negative ionization due to adductive sodium (Na+, 23 Da) and potassium (K+, 39 Da) ions with hydrogen (H+, 1 Da), when some mixed flavonoid and phenolic acid derivatives are complexly fragmented at low concentrations [24,27,28].

2.1.1. Glucosides, Malonyl-Glucosides and Acetyl-Glucosides in Raw and Steamed Seeds

From the raw and steamed soybean seeds, twenty-two glycosides were detected as structures in which glucosides (Glu, 162 Da; peaks 3, 4, 6 and 10), malonyl-glucosides (Mal-Glu, 248 Da; peaks 1115, 17, 19, 26 and 28), acetyl-glucosides (Ac-Glu, 204 Da; peaks 16, 22, 24, 27, 30, 32 and 36) and apiosyl-glucosides (Api-Glu, 294 Da; peaks 8 and 9) combined to the 7-OH or 4′-OH of daidzein (D, m/z 255; peak 33), genistein (Gn, m/z 271; peak 38) and glycitein (Gy, m/z 285; peak 35) (Figure 1 and Table 1).
Although peaks 17, 19 and 28 (m/z 503, 533, 519 [M + H]+, based on D, Gy and Gn, respectively) have been typically assigned as predominant components corresponding to ‘7-O-(6″-O-malonyl)glucoside’ from soybean seeds, the unknown isomers closely related to these compounds were still traced in ESI-MS selected ion chromatogram under affected by varietal differences [29]. Two malonylated isomers were further confirmed as ‘daidzein 7-O-(4″-O-malonyl)glucoside’ (peak 13, 4″-O-malonyldaidzin) and ‘genistein 7-O-(4″-O-malonyl)glucoside’ (peak 26, 4″-O-malonylgenistin) specified from our previous studies [24,26,27], and also consistent with LC-MS and NMR elucidation by Yerramsetty et al. [30] following novel compounds (4″-O-malonylated) derived from thermal treated standard solutions (6″-O-malonyldaidzin and 6″-O-malonylgenistin). Likewise, another isomers malonylated with the 6″-OH position of 4′-O-glucoside were indicated by their specific retention behavior and fragmentation pattern in UPLC-QToF-MS system [31], and found to be the ‘daidzein 4′-O-(6″-O-malonyl)glucoside’ (peak 11, 6″-O-malonylisodaidzin), ‘glycitein 4′-O-(6″-O-malonyl)glucoside’ (peak 14) and ‘genistein 4′-O-(6″-O-malonyl)glucoside’ (peak 25, 6″-O-malonylsophoricoside) as minor compounds [24,26,27]. Thus, six additional Mal-Glu isomers provided similar fragmentation of [M + K]+, [M + Na]+, [M + H]+ and [M + H – Mal − Glu]+ with major group (peaks 17, 19 and 28).
After steaming, Ac-Glu slightly contained in raw soybeans increased potentially as numerous isomers due to decarboxylation of Mal-Glu [32]. Seven Ac-Glu isomers (m/z 459, 489 and 475 [M + H]+, based on D, Gy and Gn, respectively) could be tentatively characterized as ‘7-O-(4″-O-acetyl)glucoside’ (peak 32), 4′-O-(6″-O-acetyl)glucoside’ (peaks 16 and 30) and ‘5-O-(6″-O-acetyl)glucoside’ (peak 22) including the well-known ‘7-O-(6″-O-acetyl)glucoside’ (peaks 24, 27 and 36) derived from corresponding Mal-Glu precursor by our recent findings [24,27].

2.1.2. New Succinyl-Glucosides and Phosphorylated Conjugates in Fermented Seeds

During cheonggukjang fermentation by B. subtilis AFY-2, succinylated and phosphorylated derivatives were newly generated from the steamed seeds [12,13], and detailed as succinyl-glucosides (Suc-Glu, 262 Da; peaks 15, 18, 20, 21, 23, 29, 31, 34 and 37) and phosphorylated conjugates (Phos, 80 Da; peaks 1, 2, 5 and 7) with combination to the 7-OH or 4′-OH position of their aglycones. Overall, Suc-Glu studies have been primarily conducted on 6″-O-succinyldaidzin and 6″-O-succinylgenistin associated with ‘7-O-(6″-O-succinyl) glucoside’ [7,8,9,13,16,26]. However, six additional isomers, including recently reported 4″-O-succinyldaidzin (peak 20) and 4″-O-succinylgenistin (peak 31) [24], were fragmented from their parent ions (m/z 517 and 533 [M + H]+, based on D and Gn) to [M + H – Suc − Glu]+ at a low level in fermented seed samples (Figure 2a–d). In particular, daidzein-succinylated 4′-O-glucoside isomers as presented in Figure 2a,b had similar characteristics with the elution order (5.60 min = 4″-O-malonylated > 5.88 min = 6″-O-malonylated) and fragment ion’s relative abundance (aglycone = [M + H-Mal-Glu]+ > parent = [M + H]+, in 4″-O-malonylated) that Yerramsetty et al. [30] and Zhang et al. [31] reported before.
Accordingly, peaks 15 and 18 were tentatively identified as ‘daidzein 4′-O-(4″-O-succinyl)glucoside’ (D4′(4″S)G, 4″-O-succinylisodaidzin) and ‘4′-O-(6″-O-succinyl)glucoside’ (D4′(6″S)G, 6″-O-succinylisodaidzin), and have not been reported in this source yet. Interestingly, the presence of these isomers suggests that microbial succinylation depends on the binding position of intact Mal-Glu and Ac-Glu described in raw and steamed seeds.
New conjugates phosphorylated to aglycones were found to be structures of daidzein 7-O-phosphate/4′-O-phosphate (peaks 1/2, m/z 335 [M + H]+) (Figure 2e,f) and genistein 7-O-phosphate/4′-O-phosphate (peaks 5/7, m/z 351 [M + H]+) with fragment ions of [M + H-H2O]+ and [M + H − Phos]+. Despite complete identification of Phos on the growth of Bacillus species in addition to reference standards [14,25], thus far, there are no experimental reports on the qualitative and quantitative determination of these derivatives via microbial phosphorylation from fermented soy products.

2.2. Changes of 38 Isoflavone Derivatives during Steaming and Fermentation in Soybean Seeds

In the present study, the internal standard material (ISTD, 6-methoxyflavone) was used to evaluate the changes of isoflavones during steaming and fermentation (after 21, 36 and 60 h with B. subtilis AFY-2) in four selected soybean cultivars. The contents (mg/100 g, dry weight) of thirty-eight isoflavone derivatives are classified into acylated group (aglycone, Glu, Mal-Glu, Ac-Glu, Suc-Glu and Phos) according to their aglycones (D, Gn and Gy) in Table 2. TIFs could be distributed differently depending on cultivar type and processed condition of soybean seeds without their loss as follows: Daewon (199.0, 261.2, 255.3, 234.0 and 241.7 for raw, steamed, 21 h, 36 h and 60 h, respectively), KLS 87248 (374.5, 382.9, 378.8, 351.3 and 371.2 for raw, steamed, 21 h, 36 h and 60 h, respectively), Nongrim 51 (299.0, 305.9, 306.6, 313.3 and 318.3 for raw, steamed, 21 h, 36 h and 60 h, respectively) and GNU-2007–14613 (196.5, 184.0, 180.6, 181.2 and 191.3 for raw, steamed, 21 h, 36 h and 60 h, respectively).

2.2.1. Glucosides, Malonyl-Glucosides and Acetyl-Glucosides during Steaming

Before steaming, TIFs of raw samples ranged from 196.5 to 374.5 agree with the seed-isoflavones variation (132.6–445.2) affected by varietal and extracted differences in previous studies [11,24,27,37]. As presented in Figure 3a,b and Table 2, the raw TIFs containing primarily Mal-Glu (77.5–84.2%) were composed of Gn (109.6–179.7, 54.5%), D (69.8–167.0, 39.0%) and Gy (8.0–27.8, 6.4%) based on their aglycones. The most significant change during steaming (60 min, 121 °C) is that predominant Mal-Glu decreased rapidly to the level of 17.0–42.1% in addition to a slight increase of TIFs compared to raw seeds [11,17]. In contrast, Ac-Glu (0–0.7%, traced) [11,27,37,38,39] and Glu (10.1–18.1%) were highly increased to 3.5–8.1% and 50.0–72.2% of TIFs, respectively, via heat-induced decarboxylation and deesterification of Mal-Glu [10,11,18], and are considered to play an intermediate role required for further conversion to aglycones (deglucosylation) in subsequent microbial fermentation.

2.2.2. Succinyl-Glucosides and Phosphorylated Conjugates during Fermentation

As cheonggukjang fermentation continued up to 60 h, the increased Ac-Glu and Glu after steaming tended to decrease gradually due to their use during succinylated and phosphorylated biosynthesis [9,13,16,25] (Figure 3b). Meanwhile, it was found that the newly generated Suc-Glu/Phos not only had a close relationship with the quantitative levels of intact TIFs in raw cultivars, but also accounted for 9.4–12.7%/0.0–0.4% (21 h), 17.3–22.4%/1.5–5.4% (36 h) and 18.7–27.4%/4.5–14.7% (60 h) of TIFs according to incubated times with B. subtilis AFY-2. Thus, Suc-Glu proportions (17.3–22.4%) at 36 h of this study were similar with previous reports presented by Toda et al. [9] (12.6%, natto), Park et al. [13] (12.2 and 18.9%, cheonggukjang and natto) and Kwon et al. [24] (20.3 and 13.6%, cheonggukjang and natto). These results indicate that most of the isoflavone aglycones and Glu were converted to Suc-Glu within about 48 h [9] (Figure 3c). In addition, the ratio (fold) of 6″-O-succinylgenistin (peak 21) to 6″-O-succinyldaidzin (peak 34) as major Suc-Glu [8,9] were changed to 1.1–1.5 (21 h), 1.2–1.9 (36 h) and 2.0–2.7 (60 h), and implied that genistin may be succinylated faster than daidzin by B. subtilis AFY-2, which agrees with the results using the corresponding Glu standards [23].
After 36 h, Phos began to increase greatly with aglycones, and their content was maximized up to 60 h (Figure 3d). The detailed bioconversion rate (%) of Phos (mainly ‘7-O-phosphates’) was recently provided as a new finding; moreover, the aglycones (daidzein:genistein, 78:96) were higher than their Glu (daidzin:genistin, 68:90). Additionally, Gn could be phosphorylated more efficiently than D at the end of 48 h incubation with B. subtilis BCRC 80517 using only standard substances [25]. Through these complementary interpretations of isoflavone standards and soy products, it can be assumed that phosphorylation was delayed rather than succinylation because most of Glu was not converted to aglycones in the early stage (21 h) of cheonggukjang fermentation (Figure 3c,d). Among the cultivars used in the present study, ‘KLS 87248′ (Korean landrace) was succinylated and phosphorylated faster than other cultivars, and expected to be superior cultivar due to its higher TIFs (351.3) with Suc-Glu (21.1%) and Phos (5.4%) levels in fermentation between 36 and 48 h, which is generally required in commercially available cheonggukjang [8,40]. However, since Phos derivatives have not been detected directly from fermented soy products using Bacillus species, their quantification is still limited in terms of precision with high reliability.

2.2.3. Proposed Roadmap on Biotransformation of Soy Isoflavones during Steaming and Cheonggukjang Fermentation

Biotransformation on soy isoflavones during steaming and fermentation (by B. subtilis AFY-2) could be proposed through a roadmap concluded from the changes of present 38 derivatives (aglycone, Glu, Api-Glu, Mal-Glu, Ac-Glu, Suc-Glu and Phos) and related previous reports [14,23,25] (Figure 4A). According to bioconversion stage (based on Gn), predominant Mal-Glu derivatives (m/z 519.113 [M + H]+) (Figure 4(Ba)) in raw seeds are decarboxylated into Ac-Glu derivatives (m/z 475.123 [M + H]+) (Figure 4(Bb)) and further deesterified into Glu during steaming. The increased Glu after steaming were mainly succinylated at the 6″-OH or 4″-OH positions of 7-O-glucoside (m/z 533.128 [M + H]+) (Figure 4(Bc)) as well as deglycosylated into aglycones during fermentation. In addition, Phos derivatives (m/z 351.026 [M + H]+) (Figure 4(Bd)) were newly formed by phosphorylation at the 7-OH or 4′-OH position of the converted aglycones. Therefore, isoflavone glycosides (Mal-Glu, Ac-Glu and Glu) were gradually hydrolyzed into their corresponding aglycones by thermal procedure (steaming) and β-glucosidase of B. subtilis AFY-2 (fermentation). After conversion to aglycones, it was found that the enhanced succinylation and phosphorylation varied depending on cultivar type and fermentation time. To enhance Suc-Glu and Phos additionally, different Bacillus species should be further characterized by means of their catalytic properties. In the future, it will be also possible to evaluate bioconversion for representative glycosidic type from other isoflavone-rich resources such as kudzu, astragalus and red clover by considering this roadmap.

3. Materials and Methods

3.1. Chemicals and Reagents

Reference standards of daidzein (≥95%), genistein (≥95%), daidzin (≥90%) and genistin (≥95%) were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA); glycitein (≥98%), glycitin (≥98%), sophoricoside (≥98%) and 6-methoxyflavone (ISTD, ≥95%) were obtained from Extrasynthese (Genay Cedex, France); 6″-O-malonyldaidzin (≥95%), 6″-O-malonylgenistin (≥90%), 6″-O-malonylglycitin (≥95%), 6″-O-acetyldaidzin (≥95%) and 6″-O-acetylgenistin (≥95%) were obtained from Synthose Inc. (Ontario, Canada); and 6″-O-acetylglycitin (99%) was obtained from MedChemExpress (Monmouth Junction, NJ, USA). LC-MS grade methanol, acetonitrile and water were supplied from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Additionally, LC grade formic acid (Junsei Chemical, Tokyo, Japan) was used to improve the efficiency of sample extraction and chromatographic separation.

3.2. Plant Materials

Among fifty-one core collected soybean cultivars with superior agronomic traits and flavonoid profiles recommended by the Gene Bank of National Agrobiodiversity Center (NAC, Jeonju, Korea), ‘KLS 87248′ (Korean landrace, rich seed-isoflavone), ‘Nongrim 51′ (Japanese breeding line, rich seed-isoflavone and leaf-flavonol), ‘GNU-2007-14613′ (Korean landrace, rich seed production in field) and ‘Daewon’ (a commercial variety commonly used in Korean cheonggukjang) were chosen considering their size (large) and yellow-coated color, which is required in seed types suitable for cheonggukjang fermentation. The selected four cultivars were sown on experimental field (19 June 2020, in rows at a spacing of 15 cm) located within the center, and cultivated under similar conditions during the country’s cropping season (June–November 2020). Their matured seeds were hand-harvested and further dried in a Bionex oven (Vision Scientific Co., Daejeon, Korea) for 72 h at 50 °C. Based on the Rural Development Administration (RDA, Jeonju, Korea) guideline, the seed size could be classified as small (<13 g), medium (13–24 g) and large (>24 g) with regard to their one hundred seeds weight [41]. Moreover, experimental research and field studies on plant materials of this study complies with relevant institutional, national and international guidelines and legislation.

3.3. Preparation of Cheonggukjang with B. Subtilis AFY-2

B. subtilis AFY-2 (KACC91988P, AFY 2) used in the present study was purchased from NUC Co. (Daegu, Korea). Cheonggukjang samples were prepared according to the method described by previous reports [8,16,22] with slight modification. Briefly, the washed seeds (1 kg) were soaked with 5 L of tap water for 12 h at room temperature and autoclaved for 60 min at 121 °C to conduct their steaming (Bionex VS-1221, Vision Scientific Co., Daejeon-Si, Korea). Steamed seeds were cooled down and inoculated with 1% (w/w) B. subtilis AFY-2, and followed by fermented for 60 h at 35 °C in an incubation room. To evaluate isoflavone changes during fermentation, cheonggukjang designed by incubation time was sampled at 0, 21, 36 and 60 h, respectively. The collected materials were lyophilized and finely grounded with a sample mill for use as analytical samples.

3.4. Extraction and UPLC-DAD-QToF/MS Analysis of Isoflavone Derivatives

With reference to Kim et al. [28] method, 1 g of powdered sample was extracted with mixed solvents (methanol:water:formic acid = 50:45:5, v/v/v, 10 mL) using an orbital shaker (for 30 min at 200 rpm), and continued centrifuging for 15 min at 2016× g and 4 °C (LABOGENE 1580R, Bio-Medical Science Co., Seoul, Korea). The collected supernatant was filtered through a 0.2 µm PVDF syringe filter (Thermo Fisher Scientific Inc., Waltham, MA, USA). Each filtrate (0.5 mL) and ISTD (6-methoxyflavone 50 ppm, 0.5 mL) were further diluted with distilled water to a final 7 mL as purpose of stable recovery during solid phase extraction (SPE). To summarize the overall SPE method, a C18 cartridge (Hypersep C18 500 mg, Thermo Fisher Scientific Inc., Waltham, MA, USA) was washed with methanol (3 mL), and followed by conditioned with distilled water (5 mL) for its initial activation. The previously diluted filtrate and ISTD were sequentially loaded on activated cartridge and washed with 5 mL of distilled water. Finally, the targeted compounds were slowly eluted from the loaded cartridge by 1% formic acid in methanol (5 mL). The semi-purified isoflavone eluate was completely concentrated using N2 gas and re-dissolved in above extraction solvent (0.5 mL) prior to UPLC-DAD-QToF/MS analysis.
A high-resolution analytical QToF/MS system (Xevo G2-S QToF, Waters MS Technologies, Manchester, UK) coupled with UPLC-DAD (ACQUITY UPLCTM system, Waters Co., Miliford, MA, USA) was operated to identify and quantify isoflavone derivatives including succinyl-glucosides and phosphorylated conjugates from the raw, steamed and fermented (cheonggukjang) samples of soybean seeds. On the basis of our previous report [42], UPLC conditions used were set followed as: 0.5% formic acid in water (eluent A) and 0.5% formic acid in acetonitrile (eluent B) used as a mobile phase in gradient program of initial (5% B), 20 min (25% B), 25 min (50% B), 30–32 min (90% B), 35–40 min (5% B, stabilization); flow rate (0.3 mL/min); sample injection volume (1 μL); CORTECS T3 C18 column (2.1 × 150 mm, 1.6 μm, Waters Co., Milford, MA, USA) and pre-column (CORTECS UPLC T3 VanGuard™, 2.1 × 50 mm, 1.6 μm, Waters Co., Milford, MA, USA); column oven temperature (30 °C). UV spectra was multi-scanned from 210 to 400 nm (254 nm for isoflavones). Mass spectra (positive ions) were simultaneously scanned with the range of m/z 100–1000 through an electrospray ionization (+ESI) probe, and their key parameters were: capillary voltage 3.5 kV, sampling cone voltage 40 V, source temperature 120 °C, desolvation temperature 500 °C, desolvation N2 gas flow 1020 L/h. All experimental analyses were carried out in triplicates.

3.5. Identification and Quantification of Isoflavone Derivatives

The previously constructed LC-MS library [27] and flavonoid database (‘RDA DB 1.0-Flavonoids’ completed in 2016, Korea) [2] (pp. 99–103) were used to clearly and efficiently identify isoflavone derivatives from the soybean seed samples (raw, steamed and cheonggukjang-fermented), and composed of twenty compounds information including positive and negative fragment ions. The purposed isoflavones were tentatively determined by comparing the positive fragmentation, UV spectra and elution order presented in the library and database. In particular, some derivatives of them were further confirmed through consistence with 13 types of reference standards provided in Table 1. Nevertheless, since it was impossible to obtain all available standards in relation to the identified derivatives, the quantification for each compound was calculated as 1:1 without considering the relative response factor for pre-inserted ISTD (based on UV detection at 254 nm).

3.6. Statistical Analysis

Data were expressed as means ± standard deviations in their triplicated results. Significant differences were determined with one-way analysis of variance (ANOVA) followed by Duncan’s multiple-range test using SPSS version 27.0 software (SPSS Inc., Chicago, IL, USA). The p-values < 0.05 were considered statistically significant.

4. Conclusions

In this work, thirty-eight isoflavone derivatives were comprehensively identified and quantified from the raw, steamed and fermented seed samples of four selected soybean cultivars based on high-resolution UPLC-DAD-QToF/MS results with reference to previously reported LC-MS library and flavonoid database (RDA DB 1.0 completed in 2016), and categorized into Glu, Api-Glu, Mal-Glu, Ac-Glu, Suc-Glu and Phos as acylated group along with D, Gn and Gy. Especially, Suc-Glu and Phos derivatives were newly generated during cheonggukjang fermentation by B. subtilis AFY-2, and among them, Phos were characterized for the first time in fermented soy products using Bacillus species. Most of the isoflavone quantification have been accurately evaluated in variation according to variety, cropping environment, storage duration and extraction solvent system of soybeans based on only 12 types of available standards (Glu, Mal-Glu, Ac-Glu and aglycones) [37,39,43]. However, since Suc-Glu (17.3–22.4%) and Phos (1.5–5.4%) have a significant proportion in 36 h-fermented cheonggukjang products, their absence can lead to a reduction of the TIFs. Additionally, the role of these derivatives should be elucidated in leading to higher isoflavone bioavailability after cheonggukjang consumption on human clinical studies [26]. Thus, the succinylated and phosphorylated isoflavones can be considered as important biomarkers generated newly during fermentation with Bacillus species, and a roadmap on isoflavone-based biotransformation proposed in this study can contribute to the enhanced production of these conjugates through metabolites regulation at each bioconversion stage. In the future, it is also necessary to carry out metabolomics approach to how isoflavone derivatives change according to type and concentration of microorganisms during fermentation as well as discover potential pharmacological activities from the Suc-Glu and Phos derivatives.

Author Contributions

Conceptualization, C.-D.W. and H.-W.K.; methodology, C.-D.W., H.-W.K., S.Y.K. and S.L.; software, S.L.; formal analysis, S.L., R.H.K., S.-J.L., H.N. and J.H.K.; investigation, S.L., J.H.K., S.Y.K. and C.-D.W.; resources, Y.-M.C., H.Y. and S.Y.K.; data curation, S.L.; Writing—original draft preparation, S.L. and H.-W.K.; Writing—review and editing, C.-D.W. and H.-W.K.; Supervision, C.-D.W., H.-W.K., Y.-M.C., H.Y., S.Y.K., Y.-S.K., S.H.L. and S.M.Y. All authors have read and agreed to the published version of the manuscript.

Funding

This study was supported by the ‘Cooperative Researcher Program for Agricultural Science and Technology Development (Project No. PJ014172012021)’, ‘RDA fellowship Program’ and ‘Collaborative Research Program between University and RDA’ of the National Institute of Agricultural Sciences, Rural Development Administration (RDA), Republic of Korea.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

Not applicable.

Conflicts of Interest

The authors declare no conflict of interest.

Sample Availability

Samples of the compounds are not available from the authors.

References

  1. Aboushanab, S.A.; Khedr, S.M.; Gette, I.F.; Danilova, I.G.; Kolberg, N.A.; Ravishankar, G.A.; Ambati, R.R.; Kovaleva, E.G. Isoflavones Derived from Plant Raw Materials: Bioavailability, Anti-Cancer, Anti-Aging Potentials, and Microbiome Modulation. Crit. Rev. Food Sci. Nutr. 2021, 1–27. [Google Scholar] [CrossRef]
  2. Kim, J.-B.; Kim, H.-W.; Lee, M.-K.; Lee, S.-H.; Kim, Y.-J.; Choi, B.-K.; Cho, S.-Y.; Kim, H.-J.; Lee, S.-H.; Jang, H.-H.; et al. Flavonoid Library. RDA DB 1.0—Flavonoids; Rural Development Administration (RDA): Wanju, Korea, 2016; pp. 59–522. [Google Scholar]
  3. Watanabe, S.; Uesugi, S.; Kikuchi, Y. Isoflavones for Prevention of Cancer, Cardiovascular Diseases, Gynecological Problems and Possible Immune Potentiation. Biomed. Pharmacother. 2002, 56, 302–312. [Google Scholar] [CrossRef]
  4. Farina, H.G.; Pomies, M.; Alonso, D.F.; Gomez, D.E. Antitumor and Antiangiogenic Activity of Soy Isoflavone Genistein in Mouse Models of Melanoma and Breast Cancer. Oncol. Rep. 2006, 16, 885–891. [Google Scholar] [CrossRef] [Green Version]
  5. Rizzo, G. The Antioxidant Role of Soy and Soy Foods in Human Health. Antioxidants 2020, 9, 635. [Google Scholar] [CrossRef]
  6. Rizzo, G.; Baroni, L. Soy, Soy Foods and Their Role in Vegetarian Diets. Nutrients 2018, 10, 43. [Google Scholar] [CrossRef] [Green Version]
  7. Toda, T.; Sakamoto, A.; Takayanagi, T.; Yokotsuka, K. Changes in Isoflavone Compositions of Soybean Foods during Cooking Process. Food Sci. Technol. Res. 2000, 6, 314–319. [Google Scholar] [CrossRef] [Green Version]
  8. Yang, S.; Lee, S.; Park, Y.; Lee, S.; Chang, P.-S.; Choi, S.-S.; Lee, J. Succinyl Daidzin and Succinyl Genistin are New Isoflavone Derivatives Found in Cheonggukjang. Food Sci. Biotechnol. 2008, 17, 172–175. [Google Scholar]
  9. Toda, T.; Uesugi, T.; Hirai, K.; Nukaya, H.; Tsuji, K.; Ishida, H. New 6-O-Acyl Isoflavone Glycosides from Soybeans Fermented with Bacillus subtilis (natto). I. 6-O-Succinylated Isoflavone Glycosides and Their Preventive Effects on Bone Loss in Ovariectomized Rats Fed a Calcium-Deficient Diet. Biol. Pharm. Bull. 1999, 22, 1193–1201. [Google Scholar] [CrossRef] [Green Version]
  10. Coward, L.; Smith, M.; Kirk, M.; Barnes, S. Chemical Modification of Isoflavones in Soyfoods during Cooking and Processing. Am. J. Clin. Nutr. 1998, 68, 1486S–1491S. [Google Scholar] [CrossRef]
  11. Lee, J.H.; Lee, B.W.; Kim, B.; Kim, H.T.; Ko, J.M.; Baek, I.-Y.; Seo, W.T.; Kang, Y.M.; Cho, K.M. Changes in Phenolic Compounds (Isoflavones and Phenolic acids) and Antioxidant Properties in High-Protein Soybean (Glycine max L., cv. Saedanbaek) for Different Roasting Conditions. J. Korean Soc. Appl. Biol. Chem. 2013, 56, 605–612. [Google Scholar] [CrossRef]
  12. Kim, M.-J.; Lee, J. Modification of Isoflavones by Processing and Photosensitization in Model and Food Systems. J. Korean Soc. Appl. Biol. Chem. 2011, 54, 833–840. [Google Scholar] [CrossRef]
  13. Park, C.U.; Jeong, M.K.; Park, M.H.; Yeu, J.; Park, M.S.; Kim, M.-J.; Ahn, S.M.; Chang, P.-S.; Lee, J. Formation of Succinyl Genistin and Succinyl Daidzin by Bacillus Species. J. Food Sci. 2010, 75, C128–C133. [Google Scholar] [CrossRef]
  14. Kim, K.-M.; Park, J.-S.; Choi, H.; Kim, M.-S.; Seo, J.-H.; Pandey, R.P.; Kim, J.W.; Hyun, C.-G.; Kim, S.-Y. Biosynthesis of Novel Daidzein Derivatives Using Bacillus amyloliquefaciens Whole Cells. Biocatal. Biotransform. 2018, 36, 469–475. [Google Scholar] [CrossRef]
  15. Lee, J.; Doo, E.-H.; Kwon, D.Y.; Park, J.-B. Functionalization of Isoflavones with Enzymes. Food Sci. Biotechnol. 2008, 17, 228–233. [Google Scholar]
  16. Park, J.; Park, M.H.; Jeong, M.K.; Kim, M.-J.; Park, K.-M.; Lee, J. Changes of Isoflavone Profiles in Cheonggukjang with Lentinus edodes. J. Korean Soc. Appl. Biol. Chem. 2012, 55, 115–119. [Google Scholar] [CrossRef]
  17. Lee, S.; Park, Y.; Han, Y.S.; Chang, P.-S.; Lee, J.-M.; Kim, Y.S.; Lee, J. Changes in Isoflavone Profiles during Cheongyukjang Preparation, A Traditional Banga Food. Korean J. Food Sci. Technol. 2009, 41, 141–145. [Google Scholar]
  18. Huang, R.-Y.; Chou, C.-C. Heating Affects the Content and Distribution Profile of Isoflavones in Steamed Black Soybeans and Black Soybean Koji. J. Agric. Food Chem. 2008, 56, 8484–8489. [Google Scholar] [CrossRef]
  19. Aguiar, C.L.; Haddad, R.; Eberlin, M.N.; Carrao-Panizzi, M.C.; Tsai, S.M.; Park, Y.K. Thermal Behavior of Malonylglucoside Isoflavone in Soybean Flour Analyzed by RPHPLC/DAD and Electrospray Ionization Mass Spectrometry. LWT-Food Sci. Technol. 2012, 48, 114–119. [Google Scholar] [CrossRef]
  20. Bao, K.; Chen, T.-L.; Zhang, S.; Huang, Z.-Z.; Huang, Y.-F.; Huang, Z.-H.; Zhu, Y.-Y.; Wu, Q.-N.; Duan, J.-A.; Zhang, Z.-Z.; et al. A Succinyl Isoflavone Identified in Natto Promotes Anti-Ischemic Effects in the Middle Cerebral Artery Occlusion Rats. J. Funct. Foods 2020, 73, 104104. [Google Scholar] [CrossRef]
  21. Jung, M.J.; Wang, M.H. Effect of Fermented Soybean-Derived Chungkookjang on Diet-Induced Hyperlipidemia in Bio F1B Hamsters. Food Biotechnol. 2009, 23, 74–82. [Google Scholar] [CrossRef]
  22. Yang, S.; Chang, P.-S.; Lee, J. Isoflavone Distribution and β-Glucosidase Activity in Cheonggukjang, a Traditional Korean Whole Soybean-Fermented Food. Food Sci. Biotechnol. 2006, 15, 96–101. [Google Scholar]
  23. Zhang, S.; Chen, G.; Chu, J.; Wu, B.; He, B. High Production of Succinyl Isoflavone Glycosides by Bacillus licheniformis ZSP01 Resting Cells in Aqueous Miscible Organic Medium. Biotechnol. Appl. Biochem. 2015, 62, 255–259. [Google Scholar] [CrossRef] [PubMed]
  24. Kwon, R.H.; Kim, H.-W.; Lee, S.; Lee, S.-J.; Na, H.; Kim, J.H.; Wee, C.-D.; Yoo, S.M.; Lee, S.H. Isoflavone Characterization in Soybean Seed and Fermented Products Based on High-Resolution Mass Spectrometry. J. Korean Soc. Food Sci. Nutr. 2021, 50, 950–961. [Google Scholar] [CrossRef]
  25. Hsu, C.; Wu, B.-Y.; Chang, Y.-C.; Chang, C.-F.; Chiou, T.-Y.; Su, N.-W. Phosphorylation of Isoflavones by Bacillus subtilis BCRC 80517 May Represent Xenobiotic Metabolism. J. Agric. Food Chem. 2018, 66, 127–137. [Google Scholar] [CrossRef] [PubMed]
  26. Jang, H.-H.; Noh, H.; Kim, H.-W.; Cho, S.-Y.; Kim, H.-J.; Lee, S.-H.; Lee, S.-H.; Gunter, M.J.; Ferrari, P.; Scalbert, A.; et al. Metabolic Tracking of Isoflavones in Soybean Products and Biosamples from Healthy Adults After Fermented Soybean Consumption. Food Chem. 2020, 330, 127317. [Google Scholar] [CrossRef]
  27. Lee, S.-J.; Kim, H.-W.; Lee, S.; Na, H.; Kwon, R.H.; Kim, J.H.; Yoon, H.; Choi, Y.-M.; Wee, C.-D.; Yoo, S.M.; et al. Characterization of Isoflavones from Seed of Selected Soybean (Glycine max L.) Resources Using High-Resolution Mass Spectrometry. Korean J. Food Nutr. 2020, 33, 655–665. [Google Scholar]
  28. Kim, H.-W.; Lee, S.H.; Yoo, S.M.; Chung, M.-N.; Kim, J.B.; Kehraus, S.; Konig, G.M. Identification and Quantification of Hydroxybenzoyl and Hydroxycinnamoyl Derivatives from Korean Sweet Potato Cultivars by UPLC-DAD-QToF/MS. J. Food Compos. Anal. 2021, 100, 103905. [Google Scholar] [CrossRef]
  29. Gu, L.; Gu, W. Characterisation of Soy Isoflavones and Screening for Novel Malonyl Glycosides Using High-Performance Liquid Chromatography-Electrospray Ionisation-Mass Spectrometry. Phytochem. Anal. 2001, 12, 377–382. [Google Scholar] [CrossRef]
  30. Yerramsetty, V.; Mathias, K.; Bunzel, M.; Ismail, B. Detection and Structural Characterization of Thermally Generated Isoflavone Malonylglucoside Derivatives. J. Agric. Food Chem. 2011, 59, 174–183. [Google Scholar] [CrossRef]
  31. Zhang, S.; Zheng, Z.-P.; Zeng, M.-M.; He, Z.-Y.; Tao, G.-J.; Qin, F.; Chen, J. A Novel Isoflavone Profiling Method Based on UPLC-PDA-ESI-MS. Food Chem. 2017, 219, 40–47. [Google Scholar] [CrossRef]
  32. Chen, Y.; Shan, S.; Cao, D.; Tang, D. Steam Flash Explosion Pretreatment Enhances Soybean Seed Coat Phenolic Profiles and Antioxidant Activity. Food Chem. 2020, 319, 126552. [Google Scholar] [CrossRef] [PubMed]
  33. Gasparetto, J.C.; Smolarek, F.S.F.; de Francisco, T.M.G.; Miranda, L.C.; Pontarolo, R.; Siqueira, P.F. Development and Validation of an HPLC-DAD Method for Analysis of the Six Major Isoflavones in Extracts from Soybean Processing. J. Am. Oil Chem. Soc. 2012, 89, 1211–1222. [Google Scholar] [CrossRef]
  34. Kudou, S.; Fleury, Y.; Welti, D.; Magnolato, D.; Uchida, T.; Kitamura, K.; Okubo, K. Malonyl Isoflavone Glycosides in Soybean Seeds (Glycine max Merrill). Agric. Biol. Chem. 1991, 55, 2227–2233. [Google Scholar] [CrossRef] [Green Version]
  35. Ohta, N.; Kuwata, G.; Akahori, H.; Watanabe, T. Isoflavonoid Constituents of Soybeans and Isolation of a New Acetyl Dadizin. Agric. Biol. Chem. 1979, 43, 1415–1419. [Google Scholar]
  36. Kudou, S.; Shimoyamada, M.; Imura, T.; Uchida, T.; Okubo, K. A New Isoflavone Glycosides in Soybean Seeds (Glycine max Merrill), Glycitein 7-O-β-D-(6”-O-Acetyl)-Glucopyranoside. Agric. Biol. Chem. 1991, 55, 859–860. [Google Scholar] [CrossRef]
  37. Lee, Y.H.; Kim, B.; Hwang, S.-R.; Kim, K.; Lee, J.H. Rapid Characterization of Metabolites in Soybean Using Ultra High Performance Liquid Chromatography Coupled with Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry (UPLC-ESI-Q-TOF-MS/MS) and Screening for α-Glucosidase Inhibitory and Antioxidant Properties Through Different Solvent Systems. J. Food Drug Anal. 2018, 26, 277–291. [Google Scholar]
  38. Lee, M.J.; Chung, I.-M.; Kim, H.; Jung, M.Y. High Resolution LC–ESI-TOF-Mass Spectrometry Method for Fast Separation, Identification, and Quantification of 12 Isoflavones in Soybeans and Soybean Products. Food Chem. 2015, 176, 254–262. [Google Scholar] [CrossRef]
  39. Kim, S.H.; Jung, W.S.; Ahn, J.K.; Chung, I.M. Analysis of Isoflavone Concentration and Composition in Soybean [Glycine max (L.)] Seeds between the Cropping Year and Storage for 3 Years. Eur. Food Res. Technol. 2005, 220, 207–214. [Google Scholar] [CrossRef]
  40. Park, M.; Jeong, M.K.; Kim, M.; Lee, J. Modification of Isoflavone Profiles in a Fermented Soy Food with Almond Powder. J. Food Sci. 2012, 71, C128–C134. [Google Scholar] [CrossRef]
  41. Choi, Y.-M.; Yoon, H.; Lee, S.; Ko, H.-C.; Shin, M.-J.; Lee, M.C.; Hur, O.S.; Ro, N.Y.; Desta, K.T. Isoflavones, Anthocyanins, Phenolic Content, and Antioxidant Activities of Black Soybeans (Glycine max (L.) Merrill) as Affected by Seed Weight. Sci. Rep. 2020, 10, 19960. [Google Scholar] [CrossRef]
  42. Lee, M.K.; Kim, H.-W.; Lee, S.-H.; Kim, Y.J.; Asamenew, G.; Choi, J.; Lee, J.-W.; Jung, H.-A.; Yoo, S.M.; Kim, J.-B. Characterization of Catechins, Theaflavins, and Flavonols by Leaf Processing Step in Green and Black Teas (Camellia sinensis) Using UPLC-DAD-QToF/MS. Eur. Food Res. Technol. 2019, 245, 997–1010. [Google Scholar] [CrossRef] [Green Version]
  43. Lee, S.J.; Ahn, J.K.; Kim, S.H.; Kim, J.T.; Han, S.J.; Jung, M.Y.; Chung, I.M. Variation in Isoflavone of Soybean Cultivars with Location and Storage Duration. J. Agric. Food Chem. 2003, 51, 3382–3389. [Google Scholar] [CrossRef] [PubMed]
Figure 1. UPLD-DAD chromatograms of 38 isoflavone derivatives (wavelength at 254 nm) from the raw, steamed and fermented (after 21, 36 and 60 h) seed samples of soybean cultivar, ‘KLS 87248’.
Figure 1. UPLD-DAD chromatograms of 38 isoflavone derivatives (wavelength at 254 nm) from the raw, steamed and fermented (after 21, 36 and 60 h) seed samples of soybean cultivar, ‘KLS 87248’.
Molecules 27 04120 g001
Figure 2. Structures and ESI(+)-QToF/MS characteristics of new succinyl-glucosides (m/z 517 [M + H]+) and phosphorylated conjugates (m/z 335 [M + H]+) based on daidzein (m/z 255 [M + H]+) generated from soybean seeds fermented (after 60 h) with B. subtilis AFY-2. (a) D4(4″S)G, daidzein 4′-O-(4″-O-succinyl)glucoside; (b) D4(6″S)G, daidzein 4′-O-(6″-O-succinyl)glucoside; (c) D7(4″S)G, daidzein 7-O-(4″-O-succinyl)glucoside; (d) D7(6″S)G, daidzein 7-O-(6″-O-succinyl)glucoside; (e) D7P, daidzein 7-O-phosphate; (f) D4P, daidzein 4′-O-phosphate.
Figure 2. Structures and ESI(+)-QToF/MS characteristics of new succinyl-glucosides (m/z 517 [M + H]+) and phosphorylated conjugates (m/z 335 [M + H]+) based on daidzein (m/z 255 [M + H]+) generated from soybean seeds fermented (after 60 h) with B. subtilis AFY-2. (a) D4(4″S)G, daidzein 4′-O-(4″-O-succinyl)glucoside; (b) D4(6″S)G, daidzein 4′-O-(6″-O-succinyl)glucoside; (c) D7(4″S)G, daidzein 7-O-(4″-O-succinyl)glucoside; (d) D7(6″S)G, daidzein 7-O-(6″-O-succinyl)glucoside; (e) D7P, daidzein 7-O-phosphate; (f) D4P, daidzein 4′-O-phosphate.
Molecules 27 04120 g002
Figure 3. Comparison in total isoflavones content (mg/100 g, dry weight) based on (a) aglycone types or (b) derivatives as well as changes in total content of new (c) succinyl-glucosides and (d) phosphorylated conjugates from soybean samples (Daewon, KLS 87248, Nongrim 51 and GNU-2007-14613) according to steamed and fermented (after 21, 36 and 60 h with B. subtilis AFY-2) conditions.
Figure 3. Comparison in total isoflavones content (mg/100 g, dry weight) based on (a) aglycone types or (b) derivatives as well as changes in total content of new (c) succinyl-glucosides and (d) phosphorylated conjugates from soybean samples (Daewon, KLS 87248, Nongrim 51 and GNU-2007-14613) according to steamed and fermented (after 21, 36 and 60 h with B. subtilis AFY-2) conditions.
Molecules 27 04120 g003
Figure 4. (A) A proposed roadmap on biotransformation of soy isoflavones during steaming and cheonggukjang fermentation (by B. subtilis AFY-2) in soybean seeds; (B) ESI(+)-QToF/MS (m/z [M + H]+) selected ion chromatograms of genistein-based (a) intact form (malonyl-glucosides, 519.113), (b) decarboxylation (acetyl-glucosides, 475.123), (c) succinylation (succinyl-glucosides, 533.128) and (d) phosphorylation (phosphorylated conjugates, 351.026) by bioconversion stage.
Figure 4. (A) A proposed roadmap on biotransformation of soy isoflavones during steaming and cheonggukjang fermentation (by B. subtilis AFY-2) in soybean seeds; (B) ESI(+)-QToF/MS (m/z [M + H]+) selected ion chromatograms of genistein-based (a) intact form (malonyl-glucosides, 519.113), (b) decarboxylation (acetyl-glucosides, 475.123), (c) succinylation (succinyl-glucosides, 533.128) and (d) phosphorylation (phosphorylated conjugates, 351.026) by bioconversion stage.
Molecules 27 04120 g004
Table 1. Characterization of thirty-eight isoflavones from the raw, steamed and fermented (by B. subtilis AFY-2) soybean seeds based on UPLC-DAD-QToF/MS.
Table 1. Characterization of thirty-eight isoflavones from the raw, steamed and fermented (by B. subtilis AFY-2) soybean seeds based on UPLC-DAD-QToF/MS.
Acylated GroupPeak No.Isoflavone DerivativesAbbreviationRT (min)Molecular FormulaObserved m/z [M + H]+Error (3) (ppm)ESI(+)-QToF-MS (Fragmented Ions of [M + H]+, m/z)References
Daidzein Derivatives (14)
Aglycone33 (2)DaidzeinD20.19C15H10O4255.06520.0277[M + Na]+, 255[M + H]+[8,19,24,26,27,29,33]
Glu4 (2)Daidzein 7-O-glucoside (Daidzin)D7G11.26C21H20O9417.1176−1.0455[M + K]+, 439[M + Na]+, 417[M + H]+, 255[M + H-Glu]+[19,24,26,27,29,33]
Mal-Glu11Daidzein 4′-O-(6″-O-malonyl)glucoside (6″-O-Malonylisodaidzin)D4′(6″M)G14.65C24H22O12503.11840.0541[M + K]+, 525[M + Na]+, 503[M + H]+, 255[M + H-Mal-Glu]+[24,26,27,31]
13Daidzein 7-O-(4″-O-malonyl)glucoside (4″-O-Malonyldaidzin)D7(4″M)G15.17C24H22O12503.11840.0541[M + K]+, 525[M + Na]+, 503[M + H]+, 255[M + H-Mal-Glu]+[24,26,27,30,31]
17 (2)Daidzein 7-O-(6″-O-malonyl)glucoside (6″-O-Malonyldaidzin)D7(6″M)G16.07C24H22O12503.1181−0.6541[M + K]+, 525[M + Na]+, 503[M + H]+, 255[M + H-Mal-Glu]+[24,26,27,29,30,31,34]
Ac-Glu16Daidzein 4′-O-(6″-O-acetyl)glucoside (6″-O-Acetylisodaidzin)D4′(6″Ac)G15.80C23H22O10459.1285−0.2497[M + K]+, 481[M + Na]+, 459[M + H]+, 255[M + H-Ac-Glu]+[24]
24 (2)Daidzein 7-O-(6″-O-acetyl)glucoside (6″-O-Acetyldaidzin)D7′(6″Ac)G18.02C23H22O10459.1285−0.2497[M + K]+, 481[M + Na]+, 459[M + H]+, 255[M + H-Ac-Glu]+[8,24,26,29,35]
Suc-Glu15 1)Daidzein 4′-O-(4″-O-succinyl)glucoside (4″-O-Succinylisodaidzin)D4′(4″S)G15.74C25H24O12517.13440.7555[M + K]+, 539[M + Na]+, 517[M + H]+, 255[M + H-Suc-Glu]+
18 (1)Daidzein 4′-O-(6″-O-succinyl)glucoside (6″-O-Succinylisodaidzin)D4′(6″S)G16.16C25H24O12517.13430.5555[M + K]+, 539[M + Na]+, 517[M + H]+, 255[M + H-Suc-Glu]+
20Daidzein 7-O-(4″-O-succinyl)glucoside (4″-O-Succinyldaidzin)D7(4″S)G16.52C25H24O12517.1333−1.7555[M + K]+, 539[M + Na]+, 517[M + H]+, 255[M + H-Suc-Glu]+[24]
21Daidzein 7-O-(6″-O-succinyl)glucoside (6″-O-Succinyldaidzin)D7(6″S)G17.10C25H24O12517.1339−0.5555[M + K]+, 539[M + Na]+, 517[M + H]+, 255[M + H-Suc-Glu]+[8,9,20,23,24,26]
29Daidzein succinyl glucoside isomerDSG19.14C25H24O12517.13420.3555[M + K]+, 539[M + Na]+, 517[M + H]+, 255[M + H-Suc-Glu]+
Phos1 (1)Daidzein 7-O-phosphateD7P8.51C15H11O7P335.03150.0335[M + H]+, 317[M + H − H2O]+, 255[M + H-Phos]+[14,25]
2 (1)Daidzein 4′-O-phosphateD4′P10.36C15H11O7P335.03232.4335[M + H]+, 317[M + H − H2O]+, 255[M + H-Phos]+[25]
Genistein Derivatives (18)
Aglycone38 (2)GenisteinGn23.58C15H10O5271.05990.7293[M + Na]+, 271[M + H]+[19,24,26,27,29,33]
Glu3Genistein 5-O-glucosideGn5G10.76C21H20O10433.11310.4471[M + K]+, 455[M + Na]+, 433[M + H]+, 271[M + H-Glu]+[24,26,27]
10 (2)Genistein 7-O-glucoside (Genistin)Gn7G14.65C21H20O10433.1124−1.2471[[M + K]+, 455[M + Na]+, 433[M + H]+, 271[M + H-Glu]+[19,24,26,27,29,33]
Api-Glu8Genistein 7-O-(6″-O-apiosyl)glucoside (Ambocin)Gn7(6″Ap)G13.73C26H28O14565.15530.2587[M + Na]+, 565[M + H]+, 433[M + H-Api]+, 271[M + H-Api-Glu]+[24,27]
9Genistein 7-O-(2″-O-apiosyl)glucosideGn7(6″Ap)G14.38C26H28O14565.15520.0587[M + Na]+, 565[M + H]+, 433[M + H-Api]+, 271[M + H-Api-Glu]+[24,27]
Mal-Glu12Genistein 5-O-(6″-O-malonyl)glucosideGn5(6″M)G15.10C24H22O13519.11330.0557[M + K]+, 541[M + Na]+, 519[M + H]+, 271[M + H-Mal-Glu]+[24,26,27]
25Genistein 4′-O-(6″-O-malonyl)glucoside (6″-O-Malonylsophoricoside)Gn4′(6″M)G18.15C24H22O13519.11360.5557[M + K]+, 541[M + Na]+, 519[M + H]+, 271[M + H-Mal-Glu]+[24,26,27]
26Genistein 7-O-(4″-O-malonyl)glucoside (4″-O-Malonylgenistin)Gn7(4″M)G18.69C24H22O13519.1128−1.0557[M + K]+, 541[M + Na]+, 519[M + H]+, 271[M + H-Mal-Glu]+[24,26,27,30]
28 (2)Genistein 7-O-(6″-O-malonyl)glucoside (6″-O-Malonylgenistin)Gn7(6″M)G19.14C24H22O13519.1130−0.6557[M + K]+, 541[M + Na]+, 519[M + H]+, 271[M + H-Mal-Glu]+[8,24,26,27,29,30,34]
Ac-Glu22Genistein 5-O-(6″-O-acetyl)glucosideGn5(6″Ac)G17.22C23H22O11475.1237−0.4513[M + K]+, 497[M + Na]+, 475[M + H]+, 271[M + H-Ac-Glu]+[24]
30Genistein 4′-O-(6″-O-acetyl)glucoside (6″-O-Acetylsophoricoside)Gn4′(6″Ac)G19.34C23H22O11475.12400.2513[M + K]+, 497[M + Na]+, 475[M + H]+, 271[M + H-Ac-Glu]+[24,26]
32Genistein 7-O-(4″-O-acetyl)glucoside (4″-O-Acetylgenistin)Gn7(4″Ac)G19.71C23H22O11475.12390.9513[M + K]+, 497[M + Na]+, 475[M + H]+, 271[M + H-Ac-Glu]+[24]
36 (2)Genistein 7-O-(6″-O-acetyl)glucoside (6″-O-Acetylgenistin)Gn7(6″Ac)G21.79C23H22O11475.12380.7513[M + K]+, 497[M + Na]+, 475[M + H]+, 271[M + H-Ac-Glu]+[24,26,27,29]
Suc-Glu31Genistein 7-O-(4″-O-succinyl)glucoside (4″-O-Succinylgenistin)Gn7(4″S)G19.62C25H24O13533.1289−0.1571[M + K]+, 555[M + Na]+, 533[M + H]+, 271[M + H-Suc-Glu]+[24]
34Genistein 7-O-(6″-O-succinyl)glucoside (6″-O-Succinylgenistin)Gn7(6″S)G20.31C25H24O13533.1288−0.3571[M + K]+, 555[M + Na]+, 533[M + H]+, 271[M + H-Suc-Glu]+[8,9,23,24,26]
37Genistein succinyl glucoside isomerGnSG22.40C25H24O13533.1286−0.7571[M + K]+, 555[M + Na]+, 533[M + H]+, 271[M + H-Suc-Glu]+
Phos5 (1)Genistein 7-O-phosphateGn7P11.31C15H11O8P351.02670.8351[M + H]+, 333[M + H − H2O]+, 271[M + H-Phos]+[25]
7 (1)Genistein 4′-O-phosphateGn4′P12.77C15H11O8P351.02681.1351[M + H]+, 333[M + H − H2O]+, 271[M + H-Phos]+[25]
Glycitein Derivatives (6)
Aglycone35 (2)GlyciteinGy21.36C16H12O5285.07590.5323[M + K]+, 307[M + Na]+, 285[M + H]+, 270[M + H-CH3]+,[19,24,26,27,29,33]
Glu6 (2)Glycitein 7-O-glucoside (Glycitin)Gy7G12.10C22H22O10447.1285−0.2485[M + K]+, 469[M + Na]+, 447[M + H]+, 285[M + H-Glu]+[19,24,26,27,29,33]
Mal-Glu14Glycitein 4′-O-(6″-O-malonyl)glucosideGy4′(6″M)G15.42C25H24O13533.1279−2.0571[M + K]+, 555[M + Na]+, 533[M + H]+, 285[M + H-Mal-Glu]+[24,27]
19 (2)Glycitein 7-O-(6″-O-malonyl)glucoside (6″-O-Malonylglycitin)Gy7(6″M)G16.49C25H24O13533.1288−0.3571[M + K]+, 555[M + Na]+, 533[M + H]+, 285[M + H-Mal- Glu]+[8,26,27,29,34]
Ac-Glu27 (2)Glycitein 7-O-(6″-O-acetyl)glucoside (6″-O-Acetylglycitin)Gy7(6″Ac)G18.69C24H24O11489.13940.5527[M + K]+, 511[M + Na]+, 489[M + H]+, 285[M + H-Ac-Glu]+[24,26,29,36]
Suc-Glu23Glycitein 7-O-(6″-O-succinyl)glucoside (6″-O-Succinylglycitin)Gy7(6″S)G17.58C26H26O13547.14500.7585[M + K]+, 569[M + Na]+, 547[M + H]+, 285[M + H-Suc-Glu]+[9,24]
All samples analyzed in positive ESI-ionization mode (m/z [M + H]+) of ToF-MS; [M + Na]+ and [M + K]+ adduct ions presented. Each peak was tentatively determined by comparing elution order, UV spectra and MS fragmentation provided from previously LC-MS and NMR reports. RT, retention time; Api or Ap (apiose, 132 Da); Glu or G (glucose, 162 Da); Ac (acetyl, 42 Da); Mal or M (malonyl, 86 Da); Suc or S (succinyl, 100 Da); Phos or P (phosphate, 80 Da); D, daidzein (m/z 255); Gn, genistein (m/z 271); Gy, glycitein (m/z 285). (1) New isoflavone derivatives in this source. (2) Further confirmed in comparison with authentic standards. (3) Error (ppm) indicates the mass accuracy of ToF data and its formula expressed as [(calculated ion − observed ion)/calculated ion] × 106 based on m/z [M + H]+ value.
Table 2. Changes in content of thirty-eight isoflavone derivatives during steaming and fermentation (after 21, 36 and 60 h) from the four selected soybean cultivars.
Table 2. Changes in content of thirty-eight isoflavone derivatives during steaming and fermentation (after 21, 36 and 60 h) from the four selected soybean cultivars.
Acylated GroupPeak No.Daewon (Control Variety)KLS 87248 (Korean Landrace)
RawSteamed21 h36 h60 hRawSteamed21 h36 h60 h
Aglycone33 (2)0.2 ± 0.1 h1.7 ± 0.2 ef1.6 ± 0.3 efg10.2 ± 2.5 c15.8 ± 0.7 b1.2 ± 0.3 efgh1.6 ± 0.1 efg1.7 ± 0.1 ef15.3 ± 0.3 b31.7 ± 0.8 a
Glu4 (2)7.0 ± 0.6 m62.2 ± 2.5 c49.8 ± 0.9 e25.0 ± 3.8 i15.3 ± 1.6 k24.3 ± 1.7 j107.4 ± 2.2 a92.9 ± 0.6 b45.8 ± 0.6 f23.2 ± 0.6 ij
Mal-Glu114.6 ± 0.2 c1.8 ± 0.0 ij1.4 ± 0.1 kl1.2 ± 0.0 lm0.9 ± 0.1 m9.5 ± 0.3 a4.6 ± 0.5 c3.7 ± 0.2 d3.1 ± 0.2 fg2.3 ± 0.1 h
130.6 ± 0.1 g0.7 ± 0.1 fg0.7 ± 0.1 fg0.9 ± 0.2 ef1.1 ± 0.2 e1.5 ± 0.1 d2.9 ± 0.3 a2.9 ± 0.2 a2.9 ± 0.1 a2.5 ± 0.2 b
17 (2)57.5 ± 2.0 c12.7 ± 0.5 h13.3 ± 0.6 h13.3 ± 0.5 h12.4 ± 1.1 h116.1 ± 6.1 a30.8 ± 2.2 ef30.8 ± 2.1 ef31.4 ± 1.5 ef28.4 ± 2.0 f
Ac-Glu16ND0.7 ± 0.0 ab0.7 ± 0.0 abc0.8 ± 0.2 a0.6 ± 0.1 cdNDND0.5 ± 0.0 deNDND
24 (2)ND5.5 ± 0.3 c4.0 ± 0.3 d2.2 ± 0.2 f1.4 ± 0.1 ghi0.8 ± 0.1 jk9.8 ± 0.6 a8.0 ± 0.6 b4.4 ± 0.1 d1.9 ± 0.3 fg
Suc-Glu15NDND0.3 ± 0.1 e0.9 ± 0.1 b1.0 ± 0.0 bNDND0.3 ± 0.1 de1.4 ± 0.1 a1.4 ± 0.1 a
18NDND0.5 ± 0.1 h1.8 ± 0.5 ef1.9 ± 0.1 deNDND1.6 ± 0.2 ef3.4 ± 0.1 b4.7 ± 0.3 a
20NDNDNDNDNDNDNDNDNDND
21NDND11.1 ± 0.6 gh14.1 ± 0.5 d11.7 ± 0.9 fgNDND13.3 ± 1.0 de25.7 ± 1.2 a23.9 ± 1.6 b
293.6 ± 0.4 bcd0.8 ± 0.5 e0.8 ± 0.6 e0.8 ± 0.5 e0.7 ± 0.5 e13.8 ± 2.2 a3.3 ± 0.1 bcde3.2 ± 0.0 bcde2.9 ± 0.1 cde2.6 ± 0.1 de
Phos1 (1)NDND1.0 ± 0.0 fg6.7 ± 0.1 de19.0 ±1.1 bNDND1.2 ± 0.2 fg12.2 ± 0.434.6 ± 3.8 a
2 (1)NDNDNDNDNDNDNDND0.8 ± 0.0 c3.6 ± 0.5 a
Total Daidzein (14)73.6 ± 3.2 h86.1 ± 2.7 f85.1 ± 1.6 f77.9 ± 2.1 gh81.9 ± 3.3 fg167.0 ± 5.5 a160.4 ± 4.4 a160.1 ± 3.8 a149.4 ± 0.9 b160.9 ± 1.7 a
Aglycone38 (2)0.3 ± 0.1 efg2.0 ± 0.2 ef1.9 ± 0.0 ef7.1 ± 2.5 c15.5 ± 0.7 b0.7 ± 0.1 fg1.2 ± 0.1 fg1.3 ± 0.0 efg6.9 ± 0.2 c16.9 ± 0.2 a
Glu30.5 ± 0.0 hi3.2 ± 0.1 c3.0 ± 0.1 d1.6 ± 0.2 f1.1 ± 0.1 g1.6 ± 0.4 f9.0 ± 0.2 a8.9 ± 0.1 a4.6 ± 0.1 b2.5 ± 0.1 e
10 (2)9.1 ± 0.7 m108.0 ± 4.2 b88.8 ± 2.2 d57.2 ± 6.2 g40.6 ± 1.4 i26.7 ± 0.9 k122.0 ± 2.4 a106.0 ± 0.3 b64.6 ± 0.9 f39.9 ± 1.1 i
Api-Glu80.2 ± 0.0 j0.4 ± 0.0 hi0.4 ± 0.0 gh0.5 ± 0.0 g0.5 ± 0.0 g1.3 ± 0.1 a0.8 ± 0.0 de0.8 ± 0.0 d0.9 ± 0.0 c1.0 ± 0.1 b
90.4 ± 0.1 i0.5 ± 0.0 hi0.6 ± 0.0 ghi0.6 ± 0.0 ghi0.6 ± 0.1 gh1.2 ± 0.0 f1.3 ± 0.0 ef1.2 ± 0.0 ef1.3 ± 0.1 ef1.4 ± 0.1 e
Mal-Glu123.5 ± 0.2 b0.8 ± 0.1 e0.8 ± 0.0 e0.5 ± 0.1 e0.5 ± 0.1 e9.6 ± 0.7 a1.8 ± 0.3 d1.8 ± 0.2 d2.1 ± 0.2 cd1.8 ± 0.2 d
255.7 ± 0.5 d1.8 ± 0.4 klm1.6 ± 0.3 mn1.1 ± 0.1 n1.1 ± 0.1 n10.7 ± 0.8 a3.7 ± 0.3 e3.1 ± 0.2 efg2.5 ± 0.3 hij1.7 ± 0.2 lm
260.8 ± 0.1 e0.5 ± 0.0 f0.5 ± 0.0 f0.6 ± 0.1 f0.6 ± 0.1 f0.3 ± 0.0 g0.9 ± 0.1 e0.8 ± 0.1 e1.0 ± 0.0 de1.0 ± 0.1 de
28 (2)89.3 ± 3.9 c23.3 ± 1.0 g24.7 ± 1.5 g26.1 ± 1.2 g25.1 ± 2.1 g123.8 ± 5.1 b34.8 ± 3.4 f35.5 ± 3.5 f38.0 ± 1.7 f37.3 ± 2.8 f
Ac-Glu22ND1.1 ± 0.1 b0.7 ± 0.2 dNDNDND1.2 ± 0.1 a1.1 ± 0.0 b0.8 ± 0.1 c0.4 ± 0.1 e
30ND0.7 ± 0.1 cd0.2 ± 0.1 eNDNDND1.0 ± 0.2 a0.7 ± 0.0 bc0.9 ± 0.2 bND
32ND0.9 ± 0.1 defND1.6 ± 0.2 b1.0 ± 0.1 cdND0.9 ± 0.1 def1.1 ± 0.1 cd2.0 ± 0.1 a1.0 ± 0.0 cde
36 (2)0.1 ± 0.0 l11.1 ± 0.1 a9.3 ± 0.3 c6.9 ± 0.5 e5.2 ± 0.2 g1.1 ± 0.2 k11.5 ± 0.2 a10.1 ± 1.0 b7.7 ± 0.3 d4.7 ± 0.6 g
Suc-Glu31NDNDND1.3 ± 0.6 de3.7 ± 0.3 bNDNDND1.3 ± 0.1 d7.1 ± 0.5 a
34NDND17.0 ± 1.0 e27.0 ± 2.3 c31.3 ± 2.3 bNDND14.4 ± 1.5 f31.0 ± 2.0 b48.6 ± 3.9 a
371.4 ± 0.1 abc1.0 ± 0.5 c0.8 ± 0.5 bc0.7 ± 0.2 bc0.9 ± 0.0 bc2.7 ± 0.0 ab2.0 ± 0.0 abc1.4 ± 0.7 abc1.6 ± 0.0 abc1.6 ± 0.1 abc
Phos5 (1)NDNDND4.7 ± 0.7 cd13.2 ± 3.1 bNDNDND6.0 ± 0.3 c14.7 ± 2.5 a
7 (1)NDNDNDNDNDNDNDNDND1.5 ± 0.1
Total Genistein (18)111.6 ± 5.3 f155.4 ± 4.0 d150.3 ± 2.7 d137.5 ± 3.7 e140.1 ± 7.3 e179.7 ± 3.6 bc192.1 ± 4.7 a188.4 ± 5.4 a173.2 ± 2.8 c183.3 ± 6.6 ab
Aglycone35 (2)ND0.8 ± 0.1 aNDND0.6 ± 0.1 bcdND0.9 ± 0.1 abc0.7 ± 0.0 ab0.4 ± 0.1 cd0.7 ± 0.1 ab
Glu6 (2)3.5 ± 0.3 l15.1 ± 0.5 c14.2 ± 0.1 d9.3 ± 0.5 f7.6 ± 0.2 h8.7 ± 0.5 g22.3 ± 0.4 a21.4 ± 0.3 b14.8 ± 0.1 c9.2 ± 0.2 fg
Mal-Glu140.9 ± 0.1 b0.4 ± 0.1 fgh0.4 ± 0.0 fg0.3 ± 0.0 ghi0.3 ± 0.1 ghi1.2 ± 0.2 a0.5 ± 0.1 efg0.5 ± 0.1 cde0.4 ± 0.1 efg0.2 ± 0.0 i
19 (2)9.5 ± 0.9 c2.3 ± 0.1 g2.5 ± 0.1 g2.6 ± 0.1 g2.5 ± 0.1 g17.7 ± 1.4 a4.9 ± 0.3 f4.8 ± 0.4 f5.1 ± 0.2 f4.8 ± 0.3 f
Ac-Glu27 (2)ND1.2 ± 0.1 c0.9 ± 0.1 d0.6 ± 0.1 f0.3 ± 0.0 ijk0.1 ± 0.0 lm1.8 ± 0.2 a1.4 ± 0.1 b0.9 ± 0.1 d0.4 ± 0.0 hij
Suc-Glu23NDND1.9 ± 0.1 gh5.8 ± 0.7 d7.5 ± 0.5 bNDND1.5 ± 0.1 hi7.0 ± 0.2 bc11.8 ± 1.5 a
Total Glycitein (6)13.8 ± 1.3 g19.8 ± 0.4 d19.9 ± 0.1 d18.6 ± 0.2 de18.8 ± 1.0 de27.8 ± 1.2 bc30.4 ± 0.4 a30.3 ± 0.9 a28.6 ± 0.5 b27.0 ± 1.9 c
Total Isoflavones199.0 ± 9.7 h261.2 ± 6.7 f255.3 ± 4.2 f234.0 ± 5.6 g241.7 ± 11.0 g374.5 ± 7.9 b382.9 ± 9.5 a378.8 ± 10.1 ab351.3 ± 4.2 c371.2 ± 9.1 ab
Acylated GroupPeak No.Nongrim 51 (Japanese Breeding Line)GNU-2007-14613 (Korean Landrace)
RawSteamed21 h36 h60 hRawSteamed21 h36 h60 h
Aglycone33 (2)0.2 ± 0.1 gh1.8 ± 0.0 ef1.4 ± 0.2 efgh6.0 ± 0.3 d16.1 ± 1.0 b1.0 ± 0.0 fgh1.3 ± 0.1 efgh0.9 ± 0.1 fgh2.5 ± 0.3 e9.9 ± 0.1 c
Glu4 (2)13.1 ± 0.5 kl64.3 ± 1.1 c55.0 ± 0.3 d36.5 ± 0.8 g24.2 ± 1.0 ij10.1 ± 2.1 l30.8 ± 1.4 h25.1 ± 0.6 i15.7 ± 0.7 k12.0 ± 0.3 l
Mal-Glu115.5 ± 0.3 b4.6 ± 0.2 c3.5 ± 0.2 de3.3 ± 0.2 ef2.8 ± 0.2 g3.8 ± 0.1 d2.8 ± 0.2 g2.3 ± 0.1 h2.1 ± 0.1 hi1.6 ± 0.2 jk
130.7 ± 0.0 fg2.0 ± 0.1 c2.0 ± 0.4 c2.4 ± 0.2 b2.6 ± 0.4 ab0.5 ± 0.1 g1.7 ± 0.1 d1.6 ± 0.1 d1.6 ± 0.2 d1.7 ± 0.1 cd
17 (2)92.0 ± 2.0 b31.1 ± 1.5 ef30.1 ± 1.8 ef33.0 ± 2.3 e29.9 ± 0.8 ef48.3 ± 2.1 d21.7 ± 1.5 g22.2 ± 1.1 g22.9 ± 1.1 g19.7 ± 1.6 g
Ac-Glu16ND0.4 ± 0.0 efNDNDNDND0.3 ± 0.1 fg0.3 ± 0.0 g0.6 ± 0.0 bcdND
24 (2)ND3.3 ± 0.2 e2.8 ± 0.3 e2.2 ± 0.2 f1.4 ± 0.2 ghi0.3 ± 0.0 kl1.5 ± 0.2 gh1.3 ± 0.2 hi1.0 ± 0.1 ij0.6 ± 0.1 jk
Suc-Glu15NDND0.4 ± 0.2 d0.9 ± 0.0 b0.9 ± 0.1 bNDNDNDND0.7 ± 0.1 c
18ND1.0 ± 0.1 g1.5 ± 0.3 f2.3 ± 0.2 d2.6 ± 0.3 cNDND1.1 ± 0.2 g1.7 ± 0.1 ef2.3 ± 0.2 d
20NDNDND0.2 ± 0.0 b0.5 ± 0.0 aNDNDNDNDND
21NDND9.3 ± 0.6 i15.8 ± 1.0 c12.8 ± 0.7 efNDND5.8 ± 0.3 j10.0 ± 0.5 hi10.1 ± 1.0 hi
295.4 ± 0.1 bc3.7 ± 0.2 bcde3.6 ± 0.1 bcde3.4 ± 0.1 bcde3.0 ± 0.1 bcde5.9 ± 0.2 b2.6 ± 0.1 de2.1 ± 0.9 de2.4 ± 0.2 de1.5 ± 0.6 de
Phos1 (1)NDND0.5 ± 0.1 fg7.8 ± 0.3 d19.0 ± 1.1 bNDNDND1.9 ± 0.3 f6.0 ± 0.2 e
2 (1)NDNDNDND1.3 ± 0.1 bNDNDNDNDND
Total Daidzein (14)116.9 ± 2.8 cd112.2 ± 0.5 de110.2 ± 1.7 e113.7 ± 3.0 cde117.1 ± 3.4 c69.8 ± 2.4 i62.6 ± 2.3 j62.6 ± 0.5 j62.5 ± 1.1 j66.1 ± 2.2 ij
Aglycone38 (2)0.2 ± 0.0 fg2.2 ± 0.2 e2.2 ± 0.3 e4.4 ± 0.2 d14.8 ± 0.2 b0.9 ± 0.1 efg1.4 ± 0.1 efg1.4 ± 0.1 efg2.0 ± 0.1 ef6.3 ± 0.1 c
Glu30.3 ± 0.0 i1.0 ± 0.1 g1.0 ± 0.1 g0.6 ± 0.0 h0.4 ± 0.1 hi0.4 ± 0.1 hi1.4 ± 0.0 f1.4 ± 0.1 f0.9 ± 0.0 g0.6 ± 0.1 h
10 (2)18.7 ± 0.9 l104.4 ± 2.1 b95.1 ± 1.4 c72.1 ± 1.5 e53.7 ± 2.3 gh15.0 ± 3.4 l49.8 ± 2.7 h41.7 ± 1.3 i31.8 ± 1.5 j28.2 ± 0.4 j
Api-Glu80.5 ± 0.0 g0.5 ± 0.0 g0.7 ± 0.0 f0.7 ± 0.0 ef0.8 ± 0.0 de0.3 ± 0.1 ijNDNDNDND
95.5 ± 0.1 d5.5 ± 0.1 d5.7 ± 0.1 c5.9 ± 0.3 b6.2 ± 0.3 a0.6 ± 0.0 gh0.6 ± 0.0 gh0.6 ± 0.0 gh0.7 ± 0.0 gh0.8 ± 0.0 g
Mal-Glu122.0 ± 0.1 cd0.9 ± 0.1 e0.9 ± 0.0 e0.8 ± 0.1 e0.6 ± 0.1 e2.3 ± 0.1 c0.8 ± 0.1 e0.8 ± 0.1 e0.9 ± 0.1 e0.8 ± 0.1 e
258.5 ± 0.2 b3.5 ± 0.4 ef3.0 ± 0.3 fgh3.1 ± 0.4 fgh2.9 ± 0.2 ghi6.3 ± 0.4 c2.7 ± 0.2 ghij2.4 ± 0.2 ijk2.2 ± 0.2 jkl1.8 ± 0.2 klm
261.6 ± 0.1 c1.8 ± 0.1 b1.6 ± 0.3 bc2.0 ± 0.2 a2.2 ± 0.1 a0.1 ± 0.0 g1.0 ± 0.1 de1.0 ± 0.1 de1.0 ± 0.1 de1.1 ± 0.2 d
28 (2)135.5 ± 8.1 a53.5 ± 3.8 e53.0 ± 4.1 e59.2 ± 5.0 e55.9 ± 4.0 e81.1 ± 5.4 d40.2 ± 1.5 f39.3 ± 2.9 f41.2 ± 2.7 f35.4 ± 0.4 f
Ac-Glu22NDNDNDNDNDNDNDNDNDND
30ND0.8 ± 0.1 b0.6 ± 0.1 cd0.5 ± 0.1 dNDNDNDNDNDND
32ND0.5 ± 0.0 g0.6 ± 0.0 f1.2 ± 0.3 c0.8 ± 0.1 efND0.3 ± 0.1 hND0.5 ± 0.1 g0.3 ± 0.0 gh
36 (2)0.2 ± 0.0 l7.3 ± 0.1 de7.0 ± 0.2 e6.2 ± 0.3 f4.7 ± 0.2 g1.0 ± 0.0 kl3.5 ± 0.1 h3.2 ± 0.2 hi2.8 ± 0.0 i2.0 ± 0.0 j
Suc-Glu31NDNDND0.9 ± 0.2 ef3.4 ± 0.4 bcNDNDND0.6 ± 0.2 f3.3 ± 0.4 c
34NDND13.4 ± 0.8 f26.0 ± 2.1 c30.7 ± 1.4 bNDND7.2 ± 0.4 g13.8 ± 1.4 f22.4 ± 2.0 d
371.1 ± 0.4 abc3.0 ± 0.1 a2.9 ± 0.2 a2.5 ± 0.1 ab2.4 ± 0.2 abc1.6 ± 0.1 abc2.2 ± 0.1 abc2.0 ± 0.1 abc1.8 ± 0.1 abc1.5 ± 0.0 abc
Phos5 (1)NDNDND4.2 ± 0.2 d12.0 ± 0.6 bNDNDND0.9 ± 0.1 f2.6 ± 0.2 e
7 (1)NDNDNDNDNDNDNDNDNDND
Total Genistein (18)174.1 ± 8.0 c184.8 ± 1.7 a187.7 ± 4.0 a190.4 ± 7.7 a191.6 ± 3.7 a109.6 ± 1.2 fg103.9 ± 2.2 g101.0 ± 2.0 g101.2 ± 3.1 g107.2 ± 5.3 fg
Aglycone35 (2)NDNDNDNDNDNDNDNDND0.4 ± 0.0 d
Glu6 (2)2.0 ± 0.2 m5.9 ± 0.1 i6.1 ± 0.0 i4.4 ± 0.1 k3.80 ± 0.1 l4.7 ± 0.6 jk10.0 ± 0.5 e8.8 ± 0.2 fg6.1 ± 0.2 i5.1 ± 0.1 j
Mal-Glu140.6 ± 0.1 c0.2 ± 0.0 hiNDNDND0.9 ± 0.1 b0.6 ± 0.0 cd0.5 ± 0.0 cdef0.6 ± 0.1 cde0.5 ± 0.1 defg
19 (2)5.3 ± 0.5 ef2.2 ± 0.1 g2.3 ± 0.1 g2.3 ± 0.1 g2.5 ± 0.0 g11.5 ± 0.6 b6.0 ± 0.1 de5.9 ± 0.4 de6.3 ± 0.3 d5.2 ± 0.3 f
Ac-Glu27 (2)ND0.6 ± 0.0 fg0.4 ± 0.0 ghi0.3 ± 0.0 jk0.2 ± 0.0 lm0.1 ± 0.0 mn0.9 ± 0.0 d0.7 ± 0.0 e0.5 ± 0.1 fgh0.2 ± 0.0 kl
Suc-Glu23NDNDND2.2 ± 0.2 g3.1 ± 0.2 fNDND1.1 ± 0.1 i4.0 ± 0.2 e6.7 ± 0.6 c
Total Glycitein (6)8.0 ± 0.7 i8.9 ± 0.1 hi8.8 ± 0.2 hi9.2 ± 0.4 hi9.7 ± 0.2 h17.1 ± 0.7 f17.5 ± 0.4 ef17.0 ± 0.4 f17.5 ± 0.5 ef18.0 ± 1.0 ef
Total Isoflavones299.0 ± 10.4 e305.9 ± 2.0 de306.6 ± 5.8 de313.3 ± 11.0 d318.3 ± 6.1 d196.5 ± 2.5 hi184.0 ± 4.8 ij180.6 ± 2.6 j181.2 ± 4.3 j191.3 ± 8.6 hij
Each value expressed as mean ± SD (n = 3). The quantification (mg/100 g, dry weight) for each peak carried out using pre-inserted internal standard (6-methoxyflavone). Different small letters in the same low with mean values indicate a significant difference at p < 0.05 by Duncan’s multiple range test. ND, not detected. (1) New isoflavone derivatives in this source. (2) Further confirmed in comparison with authentic standards. Bold numbers: importance and recognition of total contents.
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Share and Cite

MDPI and ACS Style

Lee, S.; Kwon, R.H.; Kim, J.H.; Na, H.; Lee, S.-J.; Choi, Y.-M.; Yoon, H.; Kim, S.Y.; Kim, Y.-S.; Lee, S.H.; et al. Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates. Molecules 2022, 27, 4120. https://doi.org/10.3390/molecules27134120

AMA Style

Lee S, Kwon RH, Kim JH, Na H, Lee S-J, Choi Y-M, Yoon H, Kim SY, Kim Y-S, Lee SH, et al. Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates. Molecules. 2022; 27(13):4120. https://doi.org/10.3390/molecules27134120

Chicago/Turabian Style

Lee, Suji, Ryeong Ha Kwon, Ju Hyung Kim, Hyemin Na, So-Jeong Lee, Yu-Mi Choi, Hyemyeong Yoon, So Young Kim, Yong-Suk Kim, Sang Hoon Lee, and et al. 2022. "Changes in Isoflavone Profile from Soybean Seeds during Cheonggukjang Fermentation Based on High-Resolution UPLC-DAD-QToF/MS: New Succinylated and Phosphorylated Conjugates" Molecules 27, no. 13: 4120. https://doi.org/10.3390/molecules27134120

Article Metrics

Back to TopTop