Putative Biosynthesis of Talarodioxadione & Talarooxime from Talaromyces stipitatus
Abstract
:1. Introduction
2. Materials and Methods
2.1. Fermentation and Extraction
2.2. Isolation of Metabolites
2.3. Structure Elucidation
2.4. Spectroscopic and Spectrometric Data
2.5. Bioinformatics Analysis
3. Results and Discussion
3.1. Structure Elucidation
3.2. Identification of Talaromyces Stipitatus Polyesters Biosynthetic Gene Cluster
3.3. The Biosynthesis of Talaromyces Stipitatus Polyesters
3.4. The Biosynthesis of Talarooxime
4. Conclusions
Supplementary Materials
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Position | δc (ppm) | δH (ppm), (m, J Values in Hz, nH) |
---|---|---|
1 | 110.6 | --- |
2 | 160.2 | |
3 | 102.5 | 6.37 (s, 1H) |
4 | 159.6 | --- |
5 | 113.0 | 6.19 (d, 2.2, 1H) |
6 | 139.3 | --- |
7 | 39.3 | 3.66 a (dd, 14.3, 3.8, 1H), 2.51–2.47 b (m, 2H) |
8 | 70.9 | 5.30 (dt, 6.9, 3.5, 1H) |
9 | 18.2 | 1.03 (d, 6.7, 3H) |
10 | 171.2 | --- |
11 | 41.3 | 2.86 a (t, 11.3, 1H), 2.51–2.47 b (m, 2H) |
12 | 74.5 | 5.20 (ddd, 10.8, 6.8, 3.8 Hz, 1H) |
13 | 21.0 | 1.68 (d, 6.6, 3H) |
14 | 170.7 | --- |
Gene | Gene Location | Protein Accession No. | Size | Function | Conserved Domain |
---|---|---|---|---|---|
tpeA | TSTA_008130 | XP_002488696 | 2521 aa | HR-PKS | KS-MAT-DH-ER-KR-ACP |
tpeB | TSTA_008140 | XP_002488697 | 2200 aa | NR-PKS | SAT-KS-MAT-PT-ACP1-ACP2-TE |
tpeC | TSTA_008150 | XP_002488698 | 507 aa | Oxygenase | Cytochrome P450 (CYP) |
tpeD | TSTA_008170 | XP_002488702 | 846 aa | Regulator | BED zinc finger (ZnF_BED) |
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al Fahad, A.J. Putative Biosynthesis of Talarodioxadione & Talarooxime from Talaromyces stipitatus. Molecules 2022, 27, 4473. https://doi.org/10.3390/molecules27144473
al Fahad AJ. Putative Biosynthesis of Talarodioxadione & Talarooxime from Talaromyces stipitatus. Molecules. 2022; 27(14):4473. https://doi.org/10.3390/molecules27144473
Chicago/Turabian Styleal Fahad, Ahmed J. 2022. "Putative Biosynthesis of Talarodioxadione & Talarooxime from Talaromyces stipitatus" Molecules 27, no. 14: 4473. https://doi.org/10.3390/molecules27144473