Magnesium and Zinc Oxide Nanoparticles from Datura alba Improve Cognitive Impairment and Blood Brain Barrier Leakage
Abstract
:1. Introduction
2. Results
2.1. UV Visible Spectroscopy
2.2. Functional Group Analysis by FTIR Spectroscopy
2.3. X-ray Diffraction Analysis
2.4. SEM and EDX Analysis
2.5. Impact of MgO and ZnO NPs on Learning and Spatial Memory
2.6. Effect of MgO and ZnO NPs on Working Memory
2.7. Effect of ZnO and MgO NPs on Exploratory Behavior and Locomotion
2.8. Cognition Improvement Assessments of ZnO and MgO NPs
2.9. Assessment of BBB Integrity
2.10. Impact of ZnO and MgO NPs on Body Weight
3. Discussion
4. Materials and Methods
4.1. Drugs and Chemicals
4.2. Extract Preparation
4.3. MgO NPs Synthesis
4.4. ZnO NPs Synthesis
4.5. Biosynthesized NPs Characterization
4.6. Animals and Condition
4.7. PTZ Kindling Model: In Vivo Epileptogenesis
4.8. Study Design
4.9. Neurobehavioral Studies
4.9.1. Morris Water Maze Task
4.9.2. Y-Maze Test
4.9.3. Open Field Test
4.9.4. Novel Object Recognition Test
4.9.5. Evan’s Blue Dye (EBD) Leakage Assay
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Treatment Groups | Dose mg/kg | Initial Weight (g) | Gained Weight (g) |
---|---|---|---|
PTZ | 40 | 31.01 ± 0.4079 | 34.31 ± 1.610 |
Control | Saline | 28.80 ± 1.110 | 35.01 ± 1.230 ** |
Valproate | 200 | 31.01 ± 0.4078 | 35.26 ± 0.4787 *** |
MgO | 10 | 32.01 ± 1.236 | 36.80 ± 0.9470 |
ZnO | 10 | 22.51 ± 6.560 | 33.26 ± 1.501 |
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Ullah, H.; Ullah, I.; Rehman, G.; Hamayun, M.; Ali, S.; Rahman, A.; Lee, I.-J. Magnesium and Zinc Oxide Nanoparticles from Datura alba Improve Cognitive Impairment and Blood Brain Barrier Leakage. Molecules 2022, 27, 4753. https://doi.org/10.3390/molecules27154753
Ullah H, Ullah I, Rehman G, Hamayun M, Ali S, Rahman A, Lee I-J. Magnesium and Zinc Oxide Nanoparticles from Datura alba Improve Cognitive Impairment and Blood Brain Barrier Leakage. Molecules. 2022; 27(15):4753. https://doi.org/10.3390/molecules27154753
Chicago/Turabian StyleUllah, Habib, Ikram Ullah, Gauhar Rehman, Muhammad Hamayun, Sajid Ali, Abdur Rahman, and In-Jung Lee. 2022. "Magnesium and Zinc Oxide Nanoparticles from Datura alba Improve Cognitive Impairment and Blood Brain Barrier Leakage" Molecules 27, no. 15: 4753. https://doi.org/10.3390/molecules27154753