A Novel Polysaccharide from AuriculariaAuricula Alleviates Thrombosis Induced by Carrageenan in Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Purification of AAP-b2
2.2. Monosaccharide Composition of AAP-b2
2.3. Methylation Analysis of AAP-b2
2.4. NMR Analysis of AAP-b2
2.5. Effects of AAP-b2 on Platelet Aggregation In Vitro
2.6. Effects of AAP-b2 on Thrombus Length
2.7. Effects of AAP-b2 on Coagulation Parameter
2.8. Histological Examination
2.9. Antithrombotic Pathway Analysis of AAP-b2
2.9.1. Effect of AAP-b2 on Platelet Activation in Mice
2.9.2. Effect of AAP-b2 on Anticoagulant Activity
2.9.3. Effect of AAP-b2 on Fibrinolytic Capacity
3. Materials and Methods
3.1. Materials and Reagents
3.2. Extraction and Purification of Polysaccharides
3.3. Structure Elucidation
3.3.1. Molecular Weight Determination
3.3.2. Monosaccharide Composition Determination
3.3.3. Methylation Analysis
3.3.4. NMR Spectroscopy Analysis
3.4. Animal Experiments
3.4.1. Animals and Treatments
3.4.2. Platelet aggregation
3.4.3. Determination of Thrombus Length
3.4.4. Measurement of APTT, PT, TT and FIB
3.4.5. Histological Examination
3.4.6. Enzyme-linked Immunosorbent Assay
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability Statement
References
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Groups | APTT (s) | PT (s) | TT (s) | FIB (mg/mL) |
---|---|---|---|---|
Control | 35.86 ± 0.05 | 17.60 ± 0.29 | 21.07 ± 0.48 | 1.86 ± 0.03 |
Model | 30.67 ± 0.37 ## | 13.37 ± 0.34 # | 16.90 ± 0.29 ## | 3.50 ± 0.06 ## |
Aspirin | 34.63 ± 0.09 ** | 16.27 ± 0.37 ** | 19.77 ± 0.57 ** | 2.49 ± 0.02 ** |
Test-L | 30.87 ± 0.12 | 12.70 ± 0.22 | 16.80 ± 0.29 | 3.50 ± 0.07 |
Test-M | 31.30 ± 0.37 | 12.77 ± 0.25 | 18.03 ± 0.59 | 3.24 ± 0.02 ** |
Test-H | 32.57 ± 0.29 ** | 14.00 ± 0.22 * | 18.73 ± 0.37 ** | 2.70 ± 0.02 ** |
Groups | Experimental Treatments | |||
---|---|---|---|---|
Saline | AAP-b2 | Aspirin | Carrageenan (0.5%) | |
Control | a | - | - | - |
Model | a | - | - | 10 mL/kg b.w. |
Aspirin | - | - | 20 mg/kg b.w. | 10 mL/kg b.w. |
Test-L | - | 50 mg/kg b.w. | - | 10 mL/kg b.w. |
Test-M | - | 100 mg/kg b.w. | - | 10 mL/kg b.w. |
Test-H | - | 200 mg/kg b.w. | - | 10 mL/kg b.w. |
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Bian, C.; Ji, L.; Qu, H.; Wang, Z. A Novel Polysaccharide from AuriculariaAuricula Alleviates Thrombosis Induced by Carrageenan in Mice. Molecules 2022, 27, 4831. https://doi.org/10.3390/molecules27154831
Bian C, Ji L, Qu H, Wang Z. A Novel Polysaccharide from AuriculariaAuricula Alleviates Thrombosis Induced by Carrageenan in Mice. Molecules. 2022; 27(15):4831. https://doi.org/10.3390/molecules27154831
Chicago/Turabian StyleBian, Chun, Lanyang Ji, Hang Qu, and Zhenyu Wang. 2022. "A Novel Polysaccharide from AuriculariaAuricula Alleviates Thrombosis Induced by Carrageenan in Mice" Molecules 27, no. 15: 4831. https://doi.org/10.3390/molecules27154831