Anti-Ovarian Cancer Conotoxins Identified from Conus Venom
Abstract
:1. Introduction
2. Results and Discussions
2.1. Peptide Isolation and Sequencing
2.2. Disulfide Connectivity
2.3. Blockage on Voltage-Gated Sodium Channel
2.4. Tx3a.1, Mr3.8 and Vi14b Inhibited the Proliferation of ID8 Cells
3. Materials and Methods
3.1. Venom Preparation and Peptide Isolation
3.2. Peptide Sequencing
3.3. Peptide Synthesis
3.4. Disulfide Connectivity Recognition
3.5. Patch Clamp Recording for Voltage-Gated Sodium Channel
3.6. Ovarian Cancer Cell Viability Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
References
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Name | Sequence | Origin | Disulfide Bridge | Reference |
---|---|---|---|---|
Vi14b | QQMCGRCGGTGQIIK(Ac)NECKTCHGKK(Ac) VTK | C. virgo | 2 pairs | This work |
Vi002 | LSSGATALSGVPRLTKPAGRLTTTTVAVAF * | C. virgo | none | This work |
Vi003 | NTESTKGESLLGK * | C. virgo | none | This work |
Vi15a | DCTTCAGEECCGRCTCPWGDNCSCTEW-(nh2) | C. virgo | 4 pairs | [24] |
Mr3d.1 | CCRLSCGLGCHPCC | C. marmoreus | 3 pairs | This work |
Mr3e.1 | VCCPFGGCHELCYCCD | C. marmoreus | 3 pairs | This work |
Mr3.8 | CCHWNWCDHLCSCCGS | C. marmoreus | 3 pairs | [25,26] |
Tx3a.1 | CCSWDVCDHPSCTCCG | C. texile | 3 pairs | This work |
TCP | NCPYCVVYCCPPAYCEASGCRPP | C. texile | 3 pairs | [27] |
Sequence | ya/yb | Sequence | ya/yb | Sequence | ya/yb |
---|---|---|---|---|---|
RCGGT | 475.2082 | TGQIIK(Ac) | 683.4087 | GKK(Ac) | 356.2292 |
RCGGTG | 504.2347 | IIK(Ac) | 397.2809 | GKK(Ac)V | 427.3027 |
CGGTGQ | 504.1871 | IK(Ac)N | 398.2398 | GKK(Ac)VT | 528.3504 |
CGGTGQI | 617.2712 | IK(Ac)NE | 527.2824 | KK(Ac)V | 398.2762 |
TGQI | 372.2241 | HGK | 323.1826 | K(Ac)VT | 371.2289 |
IK(Ac) | 284.1969 | GK | 186.1237 |
Sequence | ya/yb | Sequence | ya/yb | Sequence | ya/yb |
---|---|---|---|---|---|
SSG | 232.0928 | GVP | 226.1550 | LTT | 316.1867 |
SSGA | 303.1299 | PR | 226.1662 | AGRLTTT | 673.3992 |
SSGAT | 404.1776 | LT | 187.1441 | RLTTTT | 674.3832 |
GATA | 301.1506 | LTK | 315.2391 | TTTV | 403.2187 |
GATAL | 386.2398 | TKPAG | 455.2613 | TTTVA | 474.2558 |
GATALS | 473.2718 | PAG | 226.1186 | TTTTVAV | 545.3293 |
GATALSG | 558.2882 | AGR | 285.1670 | TTVAV | 472.2766 |
SGVP | 341.1819 | GRL | 299.2190 | VAVA | 341.2183 |
GV | 129.1022 | LT | 187.1441 |
Name | TP1 | TP2 |
---|---|---|
Mr3e.1 | 3.79 ± 0.61% on Nav1.4 | 21.89 ± 3.06% on Nav1.4 |
Mr3.8 | 4.62 ± 1.06% on Nav1.4 | 23.61 ± 2.70% on Nav1.4 |
Tx3a.1 | 3.17 ± 0.63% on Nav1.4 | 24.32 ± 2.29% on Nav1.4 |
TCP | 5.45 ± 0.39% on Nav1.8 | 21.51 ± 1.00% on Nav1.8 |
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Ju, S.; Zhang, Y.; Guo, X.; Yan, Q.; Liu, S.; Ma, B.; Zhang, M.; Bao, J.; Luo, S.; Fu, Y. Anti-Ovarian Cancer Conotoxins Identified from Conus Venom. Molecules 2022, 27, 6609. https://doi.org/10.3390/molecules27196609
Ju S, Zhang Y, Guo X, Yan Q, Liu S, Ma B, Zhang M, Bao J, Luo S, Fu Y. Anti-Ovarian Cancer Conotoxins Identified from Conus Venom. Molecules. 2022; 27(19):6609. https://doi.org/10.3390/molecules27196609
Chicago/Turabian StyleJu, Shuang, Yu Zhang, Xijun Guo, Qinghui Yan, Siyi Liu, Bokai Ma, Mei Zhang, Jiaolin Bao, Sulan Luo, and Ying Fu. 2022. "Anti-Ovarian Cancer Conotoxins Identified from Conus Venom" Molecules 27, no. 19: 6609. https://doi.org/10.3390/molecules27196609