Systematical Ingredient Investigations of Ficus tikoua Bur. Fruit and Immunoregulatory and Antioxidant Effects of Different Fractions
Abstract
:1. Introduction
2. Results and Discussion
2.1. Chemical Constituents
2.2. Analysis of Nutritional Phytochemicals
2.3. Antioxidant Activity
2.4. Immunomodulatory Activity
2.5. Effect of Polysaccharide on NF-κB Translocation in RAW 264.7 Cells
2.6. Effects of Polysaccharide on PI3K/AKT Signaling Pathway in RAW 264.7 Cells
3. Experimental
3.1. Materials and Chemicals
3.2. Isolation and Quantification of Main Constituents
3.3. LC–MS and GC–MS Analyses of the Chemical Components in F. tikoua Fruit
3.4. Chemical Analysis
3.4.1. Proximate Analysis
3.4.2. Vitamin Analysis
3.4.3. Amino Acid Composition
3.4.4. Total Polysaccharide Content and Its Monosaccharide Composition
3.4.5. Total Flavonoid Content (TFC)
3.4.6. Total Polyphenols Content (TPC)
3.5. Antioxidant Activity Analysis
3.5.1. DPPH Assay
3.5.2. FRAP Assay
3.5.3. ABTS Assay
3.6. Immunomodulatory Activity Analysis
3.6.1. Cell Line and Cell Culture
3.6.2. Cell Viability Assay
3.6.3. NO, PGE2, and IL-6 Production
3.6.4. Western Blot Analysis
3.7. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Gong, Y.; Luo, W.; Chen, H.; Ren, B.; Hu, W.; Li, L. Systematical Ingredient Investigations of Ficus tikoua Bur. Fruit and Immunoregulatory and Antioxidant Effects of Different Fractions. Molecules 2022, 27, 6880. https://doi.org/10.3390/molecules27206880
Gong Y, Luo W, Chen H, Ren B, Hu W, Li L. Systematical Ingredient Investigations of Ficus tikoua Bur. Fruit and Immunoregulatory and Antioxidant Effects of Different Fractions. Molecules. 2022; 27(20):6880. https://doi.org/10.3390/molecules27206880
Chicago/Turabian StyleGong, Yu, Wei Luo, Hulan Chen, Bo Ren, Weicheng Hu, and Limei Li. 2022. "Systematical Ingredient Investigations of Ficus tikoua Bur. Fruit and Immunoregulatory and Antioxidant Effects of Different Fractions" Molecules 27, no. 20: 6880. https://doi.org/10.3390/molecules27206880