Chemical Constituents and Anti-Angiogenic Principles from a Marine Algicolous Penicillium sumatraense SC29
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Characterization of Secondary Metabolites
2.2. Anti-Angiogenesis Activities in Human Endothelial Progenitor Cells
2.3. Anti-Angiogenesis Activities in an In Vivo Zebrafish Model
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Algal Material
3.3. Isolation and Identification of Fungal Stain
3.4. Extraction and Isolation of Secondary Metabolites
3.5. X-ray Diffraction Analysis
3.6. Alkaline Hydrolysis of 1
3.7. Isolation and Cultivation of Human EPCs
3.8. Cell Growth Assay
3.9. Capillary Tube Formation Assay
3.10. Cell Migration Assay
3.11. Cytotoxicity Assay
3.12. Zebrafish
3.13. Transgenic Zebrafish Lines
3.14. Embryo Collection
3.15. Angiogenesis Inhibition Drug Screening Platform
3.16. Survival Test
3.17. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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No. | 1 a,b | 2 a,b | 3 a,b | 4 a,b | 5 a,b |
---|---|---|---|---|---|
1 | 61.2, CH2 | 61.2, CH2 | 67.4, CH | 66.3, CH | 66.6, CH |
2 | 173.8, C | 172.6, C | 48.4, CH2 | 121.2, CH | 44.1, CH2 |
3 | 69.6, CH | 69.4, CH | 99.4, C | 148.2, C | 89.4, C |
4 | 41.4, CH2 | 41.3, CH2 | 202.9, C | 186.7, C | 93.1, C |
5 | 174.1, C | 173.8, C | 102.1, CH | 100.7, CH | 98.8, CH |
6 | 20.1, CH3 | 20.2, CH3 | 21.6, CH3 | 22.0, CH3 | 20.8, CH3 |
7 | 52.4, CH3 | 50.7, CH3 | 55.5, CH3 | 63.6, CH2 | |
8 | 48.4, CH3 | 57.1, CH3 | 72.5, CH | ||
9 | 55.4, CH3 | 69.2, CH2 |
No. | 1 a | 2 a | 3 a | 4 a | 5 a |
---|---|---|---|---|---|
1 | 4.07, brs | 4.07, brs | 4.11, dqd (11.4, 6.6, 3.0) | 4.82, qd (6.6, 1.8) | 4.38, dqd (11.4, 6.6, 2.4) |
2a | 2.36, dd (13.5, 3.0) | 6.02, d (1.8) | 1.67, dd (13.5, 2.4) | ||
2b | 2.65, dd (13.5, 11.4) | 1.79, dd (13.5, 11.4) | |||
3 | 5.31, dqd (8.4, 6.6, 5.4) | 5.31, dqd (7.8, 6.6, 5.4) | |||
4a | 2.56, dd (16.2, 5.4) | 2.60, dd (15.9, 5.4) | |||
4b | 2.63, dd (16.2, 8.4) | 2.66, dd (15.9, 7.8) | |||
5 | 4.94, s | 4.74, s | 4.62, s | ||
6 | 1.31, d (6.6) | 1.31, d (6.6) | 1.31, d (6.6) | 1.38, d (6.6) | 1.14, d (6.6) |
7a | 3.67, s | 3.18, s | 3.61, s | 3.72, d (12.0) | |
7b | 4.34, dt (12.0, 3.0) | ||||
8 | 3.31, s | 3.50, s | 3.77, t (3.0) | ||
9a | 3.39, s | 4.03, d (12.0) | |||
9b | 4.07, dt (12.0, 3.0) |
Compound a (µg/mL) | EPCs Growth | |
---|---|---|
20 | 40 | |
1 | >100% | >100% |
2 | >100% | >100% |
3 | 99.3 ± 1% | >100% |
4 | 65 ± 5% | 34 ± 1% |
5 | >100% | >100% |
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Hsi, H.-Y.; Wang, S.-W.; Cheng, C.-H.; Pang, K.-L.; Leu, J.-Y.; Chang, S.-H.; Lee, Y.-T.; Kuo, Y.-H.; Huang, C.-Y.; Lee, T.-H. Chemical Constituents and Anti-Angiogenic Principles from a Marine Algicolous Penicillium sumatraense SC29. Molecules 2022, 27, 8940. https://doi.org/10.3390/molecules27248940
Hsi H-Y, Wang S-W, Cheng C-H, Pang K-L, Leu J-Y, Chang S-H, Lee Y-T, Kuo Y-H, Huang C-Y, Lee T-H. Chemical Constituents and Anti-Angiogenic Principles from a Marine Algicolous Penicillium sumatraense SC29. Molecules. 2022; 27(24):8940. https://doi.org/10.3390/molecules27248940
Chicago/Turabian StyleHsi, Hsiao-Yang, Shih-Wei Wang, Chia-Hsiung Cheng, Ka-Lai Pang, Jyh-Yih Leu, Szu-Hsing Chang, Yen-Tung Lee, Yueh-Hsiung Kuo, Chia-Ying Huang, and Tzong-Huei Lee. 2022. "Chemical Constituents and Anti-Angiogenic Principles from a Marine Algicolous Penicillium sumatraense SC29" Molecules 27, no. 24: 8940. https://doi.org/10.3390/molecules27248940