Impact of Thermal Treatment on the Starch-Protein Interplay in Red Lentils: Connecting Molecular Features and Rheological Properties
Abstract
:1. Introduction
2. Results and Discussion
2.1. Impact of Heat Treatment on the Color of Red Lentil Flour
2.2. Starch Properties
2.2.1. Heat Treatment Affects Starch Susceptibility to α-Amylase Hydrolysis
2.2.2. Thermal Analysis Highlights the Modification of Both Starch and Proteins
2.2.3. Structural Modifications Lead to Different Pasting Properties
2.3. Proteins Features
2.3.1. Heat Treatment Affects Protein Structure and Aggregation
2.3.2. Water Availability in Drive Protein Modifications during Kneading
2.4. Hydration Properties
2.4.1. Thermogravimetric Analysis Shows Effects on Starch and Protein Phase Separation
2.4.2. Thermal Treatment Affects Water-Holding Capacity
2.5. Mixing Properties
3. Materials and Methods
3.1. Samples
3.2. Color
3.3. Starch Properties
3.3.1. Starch Susceptibility to α-Amylase Hydrolysis
3.3.2. Thermal Properties
3.3.3. Pasting Properties
3.4. Protein Properties
3.4.1. Protein Susceptibility to Tryptic Hydrolysis
3.4.2. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
3.4.3. Protein Differential Solubility
3.4.4. Quantification of Accessible Thiols
3.4.5. Front-Face Fluorescence
3.5. Hydration Properties
3.5.1. Thermalgravimetric Analysis (TGA)
3.5.2. Water-Holding Capacity (WHC)
3.6. Mixing Properties
3.7. Statistics
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Bresciani, A.; Emide, D.; Saitta, F.; Fessas, D.; Iametti, S.; Barbiroli, A.; Marti, A. Impact of Thermal Treatment on the Starch-Protein Interplay in Red Lentils: Connecting Molecular Features and Rheological Properties. Molecules 2022, 27, 1266. https://doi.org/10.3390/molecules27041266
Bresciani A, Emide D, Saitta F, Fessas D, Iametti S, Barbiroli A, Marti A. Impact of Thermal Treatment on the Starch-Protein Interplay in Red Lentils: Connecting Molecular Features and Rheological Properties. Molecules. 2022; 27(4):1266. https://doi.org/10.3390/molecules27041266
Chicago/Turabian StyleBresciani, Andrea, Davide Emide, Francesca Saitta, Dimitrios Fessas, Stefania Iametti, Alberto Barbiroli, and Alessandra Marti. 2022. "Impact of Thermal Treatment on the Starch-Protein Interplay in Red Lentils: Connecting Molecular Features and Rheological Properties" Molecules 27, no. 4: 1266. https://doi.org/10.3390/molecules27041266