New Insights on Rotenone Resistance of Complex I Induced by the m.11778G>A/MT-ND4 Mutation Associated with Leber’s Hereditary Optic Neuropathy
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Sequence Alignment and Conservation Determination
3.2. Structural Analyses of the Rotenone Binding Site in the ND4 Subunit
3.3. Limitations
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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ND1 | ND2 | ND3 | ND4 | ND4L | ND5 | ND6 | ||
---|---|---|---|---|---|---|---|---|
Eukaryota | Aligned sequences | 159 | 166 | 158 | 105 | 331 | 109 | 129 |
Average conservation (%) | 70 | 60 | 61 | 59 | 69 | 55 | 44 | |
Conserved residues (%) | 56 | 34 | 42 | 35 | 57 | 26 | 11 | |
Invariant residues (%) | 2 | 1 | 2 | 3 | 1 | 4 | 0 | |
Vertebrata | Aligned sequences | 109 | 127 | 101 | 62 | 279 | 59 | 84 |
Average conservation (%) | 82 | 70 | 75 | 77 | 77 | 73 | 58 | |
Conserved residues (%) | 77 | 51 | 62 | 63 | 64 | 56 | 29 | |
Invariant residues (%) | 26 | 10 | 20 | 20 | 5 | 17 | 6 | |
Mammalia | Aligned sequences | 79 | 95 | 74 | 44 | 256 | 39 | 43 |
Average conservation (%) | 85 | 77 | 80 | 83 | 80 | 79 | 71 | |
Conserved residues (%) | 79 | 62 | 70 | 77 | 69 | 68 | 55 | |
Invariant residues (%) | 39 | 20 | 31 | 38 | 17 | 32 | 23 |
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Musiani, F.; Rigobello, L.; Iommarini, L.; Carelli, V.; Degli Esposti, M.; Ghelli, A.M. New Insights on Rotenone Resistance of Complex I Induced by the m.11778G>A/MT-ND4 Mutation Associated with Leber’s Hereditary Optic Neuropathy. Molecules 2022, 27, 1341. https://doi.org/10.3390/molecules27041341
Musiani F, Rigobello L, Iommarini L, Carelli V, Degli Esposti M, Ghelli AM. New Insights on Rotenone Resistance of Complex I Induced by the m.11778G>A/MT-ND4 Mutation Associated with Leber’s Hereditary Optic Neuropathy. Molecules. 2022; 27(4):1341. https://doi.org/10.3390/molecules27041341
Chicago/Turabian StyleMusiani, Francesco, Laura Rigobello, Luisa Iommarini, Valerio Carelli, Mauro Degli Esposti, and Anna Maria Ghelli. 2022. "New Insights on Rotenone Resistance of Complex I Induced by the m.11778G>A/MT-ND4 Mutation Associated with Leber’s Hereditary Optic Neuropathy" Molecules 27, no. 4: 1341. https://doi.org/10.3390/molecules27041341