Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effects of α-T, TS, and TP on the Viability of FLS
2.2. Effects of α-T, TS, and TP on the Expression of Rheumatoid Arthritis-Related Genes
2.3. Anti-RA Effects of TP on Laminarin-Induced RA Model Mice
3. Materials and Methods
3.1. Materials
3.2. Evaluation of Cell Viability by WST-1 Assay
3.3. Determination of mRNA Levels by RT-PCR
3.4. Quantification of TP Amounts in the Cell Culture Medium by HPLC
3.5. Animal Experiments
3.6. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Hama, S.; Kirimura, N.; Obara, A.; Takatsu, H.; Kogure, K. Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice. Molecules 2022, 27, 1425. https://doi.org/10.3390/molecules27041425
Hama S, Kirimura N, Obara A, Takatsu H, Kogure K. Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice. Molecules. 2022; 27(4):1425. https://doi.org/10.3390/molecules27041425
Chicago/Turabian StyleHama, Susumu, Naoko Kirimura, Aki Obara, Hirokatsu Takatsu, and Kentaro Kogure. 2022. "Tocopheryl Phosphate Inhibits Rheumatoid Arthritis-Related Gene Expression In Vitro and Ameliorates Arthritic Symptoms in Mice" Molecules 27, no. 4: 1425. https://doi.org/10.3390/molecules27041425