Nanotechnology, Green Synthesis and Biological Activity Application of Zinc Oxide Nanoparticles Incorporated Argemone Mxicana Leaf Extract
Abstract
:1. Introduction
2. Results and Discussion
2.1. X-ray Diffraction Analysis
- k = Scherrer’s constant (0.9)
- λ = Wavelength of X-ray (1.54 × 10−10 m)
- β = Full Width Half Maximum
- θ = Bragg’s angle
2.2. FTIR Analysis
2.3. Scanning Electron Microscopic Analysis (SEM)
2.4. Energy-Dispersive X-ray Spectroscopy
2.5. Ultraviolet—Visible (UV-Vis) Spectroscopy
2.6. Antibacterial Activity
2.7. Antifungal Activity
2.8. Antioxidant Activity
3. Materials and Methods
3.1. Preparation of Leaf Extract
3.2. Biosynthesis of Zinc Oxide Nanoparticle
3.3. Antibacterial Activity Assay
3.4. Antifungal Activity Assay
3.5. Antioxidant Activity
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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S.NO | 2 θ Degree | d A° | FWHM (deg) | Hkl | Crystalline Size (nm) | Average Crystal Size (nm) |
---|---|---|---|---|---|---|
1. | 31.737 | 2.820 | 0.56300 | 100 | 1.47129 | 1.461449 |
2. | 34.379 | 2.608 | 0.54300 | 002 | 1.532493 | |
3. | 36.215 | 2.480 | 0.60570 | 101 | 1.380564 |
Peak Value | Chemical Bonding |
---|---|
871 cm−1 | C=C bending |
3196 cm−1 | O=H Stretching |
1626 cm−1 | C=C Stretching |
1650 cm−1 | C=N Stretching |
1102 cm−1 | C-O Stretching |
Sample | At of (Zn) | At of (O) |
---|---|---|
ZNp | 32.05 | 34.58 |
Sample No | Sample Marking | Sample Concentration | Test Organisms and Zone of Inhibition in (mm) | |
---|---|---|---|---|
Staphylococcus aureus | E. coli | |||
2 | Control | MHA | NA | NA |
3 | Leaf Extract | 75 µL | NA | 8 mm |
4 | 100 µL | 7 mm | 11 mm |
Sample No | Sample Marking | Sample Concentration | Zone of Inhibition in (mm) |
---|---|---|---|
Aspergillus fumiga | |||
2 | Control | PDA | NA |
3 | ZnO AML | 25 µL | 5 mm |
4 | 75 µL | NA | |
5 | Leaf Extract | 50 µL | NA |
6 | 100 µL | 13 mm |
Sample No | Extract Concentration (μg/mL) | DPPH Antioxidant Activity | ||
---|---|---|---|---|
OD Value at 517 nm (in Triplicates) | ||||
1 | 500 μg/mL | 1.613 | 1.244 | 1.507 |
2 | 250 μg/mL | 0.108 | 0.120 | 0.122 |
3 | 100 μg/mL | 0.152 | 0.153 | 0.198 |
4 | 50 μg/mL | 0.211 | 0.236 | 0.247 |
5 | 10 μg/mL | 0.283 | 0.253 | 0.273 |
6 | Control | 1.189 | 1.148 | 1.116 |
Sample No | Standard | Concentration (g/mL) |
Antioxidant Activity % of Inhibition |
---|---|---|---|
DPPH (510 nm) | |||
1 | Ascorbic acid used as a standard (OD value) | 500 μg/mL | 91.97 |
2 | 250 μg/mL | 88.46 | |
3 | 100 μg/mL | 84.08 | |
4 | 50 μg/mL | 81.44 | |
5 | 10 μg/mL | 20.83 |
Day | Materials and Methods | Process |
---|---|---|
I | 3.8 g of Zinc acetate dihydrate+ 50 mL of distilled water | Stirred for 30 min. |
10 g leaves of Argemone maxicana+50 mL of distilled adding 60 mL of leaf extract (drop wise) | Stirred for 20 min + Filtering extract with whatmann filter paper. | |
Formation of Zinc nanoparticles (deep emerald green color) | 1hour of stirring (PH = 12) and then kept in room temp. | |
II | Deep emerald green precipitation was formed | Per day for Two times water changed. |
III | Deep emerald green precipitation was formed | Per day for Two times water changed. |
IV | Dried in hot air oven at 100 °C for Five hour | Sample transferred to silica crucible cup. |
V | Heated in muffle furnace at 400 °C for 2 h. White precipitation was formed finally. | Grained in mortar ZnO Nano powder. |
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Chinnapaiyan, M.; Selvam, Y.; Bassyouni, F.; Ramu, M.; Sakkaraiveeranan, C.; Samickannian, A.; Govindan, G.; Palaniswamy, M.; Ramamurthy, U.; Abdel-Rehim, M. Nanotechnology, Green Synthesis and Biological Activity Application of Zinc Oxide Nanoparticles Incorporated Argemone Mxicana Leaf Extract. Molecules 2022, 27, 1545. https://doi.org/10.3390/molecules27051545
Chinnapaiyan M, Selvam Y, Bassyouni F, Ramu M, Sakkaraiveeranan C, Samickannian A, Govindan G, Palaniswamy M, Ramamurthy U, Abdel-Rehim M. Nanotechnology, Green Synthesis and Biological Activity Application of Zinc Oxide Nanoparticles Incorporated Argemone Mxicana Leaf Extract. Molecules. 2022; 27(5):1545. https://doi.org/10.3390/molecules27051545
Chicago/Turabian StyleChinnapaiyan, Maheswari, Yashika Selvam, Fatma Bassyouni, Mathammal Ramu, Chandrasekar Sakkaraiveeranan, Aravindan Samickannian, Gobi Govindan, Matheswaran Palaniswamy, Uthrakumar Ramamurthy, and Mohamed Abdel-Rehim. 2022. "Nanotechnology, Green Synthesis and Biological Activity Application of Zinc Oxide Nanoparticles Incorporated Argemone Mxicana Leaf Extract" Molecules 27, no. 5: 1545. https://doi.org/10.3390/molecules27051545