Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules
Abstract
:1. Introduction
Disorder | Plant Part | Dosage Form | Region/Country | References |
---|---|---|---|---|
B. pilosa | ||||
Stomach ache | LE | Not stated | Africa | [11] |
Colic | WP | Decoction | China, Africa | [11] |
Catarrh | WP | Juice | Cuba | [12] |
Diarrhea | LE, WP | Decoction | Uganda, Africa | [13] |
Constipation | WP | Decoction | India | [14] |
Dysentery | WP | Infusion | Africa | |
Choleretic | WP | Decoction | America | [15] |
Antirheumatic | RT, WP | Infusion | Hong Kong | [16] |
Appendicitis | WP | Not stated | Hong Kong | [16] |
Enteritis | WP | Decoction | China | [17] |
Otitis | WP | Decoction | China, Africa | [18] |
Gastritis | WP | Juice | Cuba | [19] |
Diabetes | WP | Decoction | Taiwan, Cuba | [11] |
Headache | WP | Decoction | Bafia, Cameroon | [11] |
Diuretic | WP | Decoction | Central America | [20] |
Hypotensive | WP | Juice | Cameroon | [21] |
Fever | WP | Decoction | Not stated | [19] |
Yellow Fever | LE, WP | Decoction | America | [13] |
Acute hepatitis | WP | Decoction | Hong Kong | [22] |
Intestinal worms | LE | Decoction | Africa | [14] |
Malaria | WP | Juice | China | [18] |
Eye diseases | LE | Juice | Uganda | [13] |
B. biternata | ||||
Leprosy | LE | Not stated | India | [23] |
Cuts and wounds | LE | Decoction | India | [23] |
Nose bleeds | WP | Decoction | China | [23] |
Gastric ulcers | LE | Maceration taken orally | Central America | [10] |
Skin problems | WP | Topical application | Africa | [24] |
Wounds | WP | Crushed herb | China | [24] |
Snake bites | WP | Crushed herb | China | [23] |
B. bipinnata L. | ||||
Asthma | WP | Decoction | China | [25] |
Colds | LE | Decoction | China | [25] |
Fever | WP | Decoction | Not stated | [9] |
Antimicrobial | AP | Decoction | Trinidad | [26] |
Eye Diseases | LE | Juice | Uganda | [13] |
Colds | LE, WP | Decoction | Uganda, China | [21] |
2. Material and Method
2.1. Formation of Crude Methanolic Plant Extract
2.2. In-Vitro Cytotoxicity Assay
Thiazolyl Blue Tetrazolium Bromide (MTT)
2.3. In-Vivo Cytotoxicity Assay Caenorhabditis elegans
2.4. In Vivo Cytotoxicity Assay on Zebrafish
2.5. In Vivo Cytotoxicity Assay on Brine Shrimp
2.6. Bioassay-Guided Purification
2.7. Identification of Isolated Compound
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Zahara, K.; Bibi, Y.; Masood, S.; Nisa, S.; Sher, A.; Ali, N.; Kumar, S.; Qayyum, A.; Ahmed, W.; Sami, R.; et al. Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules. Molecules 2022, 27, 1927. https://doi.org/10.3390/molecules27061927
Zahara K, Bibi Y, Masood S, Nisa S, Sher A, Ali N, Kumar S, Qayyum A, Ahmed W, Sami R, et al. Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules. Molecules. 2022; 27(6):1927. https://doi.org/10.3390/molecules27061927
Chicago/Turabian StyleZahara, Kulsoom, Yamin Bibi, Saadia Masood, Sobia Nisa, Ahmad Sher, Naushad Ali, Sunjeet Kumar, Abdul Qayyum, Waseem Ahmed, Rokayya Sami, and et al. 2022. "Isolation and Identification of Bioactive Compounds from Bidens spp. Using HPLC-DAD and GC-MS Analysis and Their Biological Activity as Anticancer Molecules" Molecules 27, no. 6: 1927. https://doi.org/10.3390/molecules27061927