1. Introduction
Roselle is one of the potential sources of anthocyanins as red pigments [
1]. Roselle is cultivated widely in tropic and sub-tropic countries, such as Indonesia, Malaysia, China, Mexico, Thailand, and Egypt [
2]. Roselle is brightly red colored and rich in nutrients such as organic acid, anthocyanins, and pectin. Grajeda-Iglesias, et al. [
3] also identified that roselle is rich in anthocyanins as red natural pigments. Cyanidin 3-glucoside and delphinidin 3-glucoside are the most abundant anthocyanins present in the calyces of roselle [
4].
Anthocyanins categorized as flavonoids are water-soluble pigments that can be found in most vascular plants. Anthocyanins have been revealed as one of the most promising ingredients in the beverage, cosmetics, food, and nutraceutical industries. This bioactive compound can improve the control of Type II diabetes and coronary heart disease and prevent cancer development [
5]. Anthocyanins as high polar compounds are found predominantly in inner cell layers such as the epidermis and peripheral mesophyll cells [
6,
7,
8,
9,
10].
In response to a growing awareness of the detrimental environmental effects of synthetic products, the use of sustainable natural products has become more important [
11]. To produce great extraction yields from natural materials, it is vital to choose the most suited extraction techniques and circumstances. Natural colorants have gained popularity due to their properties and health-promoting advantages, as well as their low cytotoxicity in comparison to synthetic colorants [
12]. These natural colorants have a wide range of potential uses, notably in the food, textile, and pharmaceutical industries as shown in
Figure 1 [
13,
14,
15].
Supercritical carbon dioxide (ScCO
2) is often used to extract bioactive compounds, such as phenolic, flavonoid, and antioxidant compounds [
16,
17,
18]. This method is a novel approach for optimizing the extraction of chemicals of interest, especially for anthocyanins since it is a non-hazardous and eco-friendly solvent. Mohd-Nasir, et al. [
19] and Abdul Aziz, et al. [
20] reported that this technology has many benefits over traditional extraction techniques, including a reduction in extraction time, an improvement in extract quality, reduced extraction agent costs, and an eco-friendly operation. This technique is rapidly gaining acceptance as an alternative to solid sample extraction [
21,
22,
23]. It is also relatively recent and quite promising [
24].
However, pure carbon dioxide cannot be applied to extract anthocyanins from roselle [
11,
25,
26,
27,
28,
29,
30]. Commonly, ethanol is applied as an entrainer/modifier for supercritical carbon dioxide to extract anthocyanins. According to the United Nations’ sustainable development goals for good health and well-being, ethanol is classified as a generally recognized as safe (GRAS) solvent, as it belongs to Class 3 in the Guidance for Industry of the US Food and Drug Administration (FDA), which means it is considered non-hazardous.
This review contrasts the ScCO2 approach with conventional extraction techniques to highlight the benefits and drawbacks of ScCO2 in comparison to conventional methods. In addition, the concepts and mechanics of ScCO2 are described, as well as the evolution of ScCO2 technology up to the current day. The optimization of several extraction parameters, such as the co-solvent ratio, flow rate, extraction time, temperature, raw matrix, and pressure, are examined in depth. This article also included anthocyanins as red pigments for natural colorants. In addition, the appropriate ScCO2 extraction conditions are presented, along with a prospective outlook on the commercialization of roselle as a natural colorant source.
2. Anthocyanins as Red Pigments
Due to their great stability (light, oxygen, heat, and pH), homogeneity, coloring power, independence from microbial contamination, and comparatively cheap manufacturing cost, synthetic colorants are gradually replacing natural colorants. However, the usage of synthetics is decreasing progressively owing to their possible health risks [
31]. The issue surrounding the use of chemicals in consumables is rooted in the tension between technical need and safety, as well as the advantages and possible toxicological dangers of long-term exposure to these compounds [
32]. It has been observed that the growth in consumer understanding about what they eat is the primary reason in lowering the usage of synthetic chemicals [
14]. In addition, studies have highlighted the toxicity of food colorings since 1960, which has aroused grave worry among global health authorities (
Table 1).
Anthocyanins can be obtained from various plant sources such as tea, wines, nuts, fruits, vegetables, blackcurrant, cocoa, cereals, and honey. Ali, et al. [
40] reported that the molecular formula of anthocyanins is C
15H
11O
+, whereas its molecular weight and melting point are 207.24 g/mol and 150 °C, respectively. Despite its high melting point, anthocyanins will degrade at a temperature of 80 °C [
41], and it was also revealed that the degradation in anthocyanins was observed when treated at 80 °C. The anthocyanins structure links hydroxyl (−OH) and or methoxyl (−OCH
3) groups and one or more sugars as shown in
Figure 2 [
42].
Table 2 shows that roselle contains 335.7 mg/g anthocyanins extracted using Soxhlet extraction with various different ratios of fresh roselle to water (1:10 g/mL), which were conducted using a water bath at a temperature of 60 °C for 60 min [
43]. The anthocyanin concentration for roselle is higher than for strawberries and blueberries [
10]. Although the anthocyanins concentration in roselle is lower than in cherries, this is due to the other isomerization of anthocyanins being dominant to increase the antioxidant activity [
44].
Extraction is the separation of bioactive plant compounds from their inert components using selected solvents, which is the first step necessary to obtain the appropriate chemical components for further chemical analysis [
24]. Pre-treatment, purification, and analysis are important steps in the extraction procedure. Traditional extraction methods are commonly categorized as a leaching process to separate extract from the solid (solid–liquid) [
49,
50]. Conventional methods include Soxhlet extraction and maceration extraction [
51,
52]. These techniques have been utilized for more than a century for the extraction, isolation, and purification of anthocyanins [
29,
30,
42,
53,
54].
Anthocyanins are located in the inner cells of fruits and vegetables [
55]. Acidic solvents are commonly used to denature the membranes of cell tissue and dissolve the pigments. Alappat, et al. [
8] and Silva, et al. [
30] reported that the acid has a tendency to stabilize anthocyanins, but it may also alter the natural form of pigment in the tissue by severing bonds with metals, pigments, or other variables. The recent research of solid–liquid extraction of anthocyanins from roselle calyces was performed by Wu, et al. [
54]. They used 30% (
v/
v) ethanol in a 1:20 (
w/
v) ratio, followed by extraction at 75 °C for 20 min in a water bath, which resulted in about 361.99 mg/100 g of anthocyanins content. Meanwhile, the optimization model from the study by Miranda-Medina, et al. [
56] showed that 96% ethanol, 65 °C, and a 1:50 calyx-to-solvent ratio gave the highest anthocyanins concentration of 150 mg/100 g.
3. Conventional Extraction of Anthocyanins
Conventional extraction of anthocyanins from roselle is nonselective and produces considerable quantities of by-products, such as sugars, organic acids, alcohols, organic acids, proteins, and amino acids [
11,
43,
57]. Some of these contaminants, such as sugars, hasten the decomposition of anthocyanins during storage [
58]. Efficiency in the extraction of anthocyanins can be maximized with the optimization parameter for a few degradation compounds. Additionally, the extraction technique should not be dangerous, too complex, time-consuming, or expensive. For the extraction of anthocyanins, methanol, ethanol, and acetone are more effective than using other solvents. However, solvents such as methanol/acetone may be hazardous to human health [
59]. Therefore, the exploration of greener techniques for the extraction of anthocyanins is essential for current research [
49].
Silva, et al. [
30] have identified the benefits and drawbacks of anthocyanin extraction strategies using solid–liquid extraction, ScCO
2, pressurized liquid extraction (PLE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE). The solid–liquid extraction process involves a long extraction time, and within that time, exposure to acid, air, and light may cause anthocyanin degradation. In the UAE method, energy dissipation through heat may also cause anthocyanin degradation. Meanwhile, PLE and MAE require high temperatures during extraction which also contributes to degradation. The quality of natural extracts is significantly related to the extraction method, solvents used, vegetal matrix characteristics, and storage conditions. The selection of solvents must be carefully selected to maximize the yield and selectivity [
29,
60,
61].
Even though ScCO
2 extraction is a green extraction method, as it uses a nontoxic solvent, it is not suitable for anthocyanin recovery due to a difference in polarity between CO
2 and anthocyanins. Currently, ethanol has been used as a co-solvent in order to enhance the polarity of CO
2 [
62,
63]. Therefore, anthocyanins can be extracted using ScCO
2 and ethanol as a co-solvent [
11]. However, a greater concentration of co-solvent in the system would contribute to the solvent–solvent interactions in competition for the solvation of solutes, hence reducing the recovery [
62].
4. ScCO2 as Green Extraction
Extraction is the process of separating constituents from natural substances using chemical or physical means. As a part of its efforts to conserve the environment, the global community has recently adopted green extraction [
64,
65]. ScCO
2 as green extraction is based on methods that take less energy, permit the use of alternative solvents and renewable resources, and provide extracts that are both safe and high quality. It is regarded as the most effective alternative to conventional solvent extraction techniques for bioactive compounds. The four principles of eco-friendly natural product extraction are as follows [
66,
67]:
Renewable plant materials;
Use of alternative solvents and safe solvents;
Reduction in energy consumption;
Production of co-products as opposed to trash, including bio- and agro-refining industries.
To preserve natural materials, ScCO2 extraction requires either intensive culture or in vitro growth of plant cells or organisms. Natural colorants may be extracted from plants, animals, and fungi while preserving the rights of future generations. Using this method, it is possible to manufacture active components without solvent residue. CO2, which is widely utilized in the supercritical fluid extraction (SFE) technique, is an inflammable, odorless gas produced by the combustion of fossil fuels, the fermentation of alcohol, and human and animal respiration. In lieu of organic solvents such as hexane, the SFE approach employs compressed scCO2 at a pressure of up to 300 MPa and a temperature of 30 to 40 °C in the extraction process. Extraction is impacted by economic and environmental challenges, which need a significant reduction in energy use and waste generation.
Compared to conventional extraction procedures, the SFE approach expedites the extraction process. No further actions are necessary to save time or energy. A vast array of coproducts, by-products, and trash are generated during the extraction activities. According to the idea of biorefinery, plant resources are used in an integrated fashion. Plants contain several refined substances. Each component of a plant may be extracted and utilized to manufacture an assortment of goods. By creating functionalize co-products, the SFE process at low temperatures permits the identification of novel chemicals that improve the value of the extract. Reducing the number of steps in a manufacturing chain reduces costs and optimizes energy use. It indicates that the ideal approach is a one-step process. The advantages of supercritical fluid extraction include the use of a clean solvent and the production of an extract with minimum discrete processes. Furthermore, the extract is guaranteed to be harmless to people and the environment. Using this approach, the SFE method safeguards thermally unstable target molecules. In addition, the SFE extract has no residual solvent, making it very pure. Furthermore, the solvent of SFE can be recycled using a compressor, thus it will reduce the production cost [
64].
5. ScCO2 as a Solvent for Anthocyanin Extraction
Carbon dioxide (CO
2) has been used as a supercritical fluid for centuries. It is chosen based on its unique properties as discussed earlier. A comparison of its physical properties to some other fluids is shown in
Table 3. Due to its low critical temperature, it can operate at moderate temperature, hence preventing substance degradation due to its heat induction [
68]. CO
2 is the preferred supercritical solvent for the extraction of bioactive compounds because it is colorless, odorless, non-flammable, inert, non-toxic, safe, recyclable, and can be utilized under mild operational conditions (critical temperature of 31.1 °C and critical pressure of 7.38 MPa). Furthermore, this method aims for high selectivity, high product purity, and low extraction time, while being non-hazardous to human health and the environment.
The main factors of a supercritical fluid are the critical pressure (Pc), critical temperature (Tc), the critical density and solubility of the fluid. The density of CO2 can be manipulated using different pressures and temperatures. Around the Pc, a significant change in density will occur in the enhancement of pressure. The solute solubility in a supercritical fluid is commonly influenced by the density of the solvent. Thus, a significant change in solubility is anticipated with an increase in pressure from the subcritical level (P < Pc) to the supercritical pressure level (P > Pc).
6. Parameter Effects on ScCO2 Extraction
Optimizing conditions to produce an adequate extraction of bioactive compounds while preventing undesired components is the initial stage of the ScCO
2 procedure. Optimizing the operational parameters is a crucial stage in the development of the ScCO
2 technology since numerous process factors might impact the extraction efficiency. Several SFE parameters, which may be adjusted [
57], are required for the successful extraction of bioactive components from plant materials.
Table 4 shows the optimal conditions to extract the rich anthocyanins from roselle.
Several factors have been proven to have significant effects on the efficiency of ScCO
2 including the type of sample, fluid, pressure, flow rate, temperature, and extraction time. Reverchon, et al. [
69] previously reported that all those parameters could affect the extraction rate. However, the main parameters that contribute to the extraction performance are pressure and temperature. In addition, the moisture content in the sample matrix and the sample and particle size as well as solvent flow rate will ultimately affect the extraction performance.
Table 4.
The critical parameters in the ScCO2 extraction in the anthocyanin recovery.
Table 4.
The critical parameters in the ScCO2 extraction in the anthocyanin recovery.
Parameter | The Optimal Range | Ref. |
---|
Temperature | Temperature 40 to 70 °C | [11,70,71] |
Pressure | Above 20 MPa |
Co-solvent | Ethanol (15%), combination ethanol and water |
Time | Less than 3 h |
Flowrate | 1 to 10 mL/min, preferable slow flow rate to enhance the residence time of extraction |
Particle size | 0.25 < dp < 1.80 mm |
Moisture content | 4–14% |
Pre-treatment | Freeze-dried/sun dried |
6.1. Effect of Pressure at Constant Temperature in ScCO2 Extraction
Pressure is one of the important elements governing the ScCO
2 process due to its influence on the solubility of compounds and diffusivity of the solvent [
20,
62,
72]. Utilizing a back pressure regulator that maintains the desired pressure of ScCO
2 may be used to conduct pressure control in the ScCO
2 approach. At a fixed temperature, raising the pressure enhances the solubility of the bioactive compounds and the extraction yield by increasing the solvent power and extraction efficiency. In addition, increasing pressure enhances the density. As a result, the solubility improves, resulting in a greater recovery of anthocyanins [
25]. If the pressure is raised above a certain point, the solvent diffusivity may decrease. Additionally, there may be less interaction with the pores of the raw material, which may lead to a reduction in solute dissolution [
25]. On the other hand, a higher-pressure condition is not suggested for all targeted compounds as it might compress the raw material, hence decreasing the extraction yield [
6]. The effect of pressure is also associated with the increase or decrease in solvent power due to the density of the solvent and by strengthening the intermolecular physical interaction [
73].
6.2. Effect of Temperature at Constant Pressure in ScCO2 Extraction
The temperature has two distinct impacts at constant pressure. The enhancement of temperature decreases the density and solvating capacity of carbon dioxide. However, raising the temperature improves the solubility and extraction yield of the target compounds by increasing their vapor pressure. Consequently, this may result in the crossing of the isotherms, a process known as retrogradation in which higher-temperature conditions result in low yields and low temperatures result in a higher yield [
11,
20,
25]. The inversion of yield isotherms is a result of these competing influences on the overall extraction yield.
By analyzing the crossover property, Cockrell, et al. [
74] hypothesized that the density effect dominates at pressures below the crossover pressure, while the solute vapor pressure controls the extraction process at higher pressures. A greater temperature decreases the extraction recovery of nonvolatile substances. However, their solubility in ScCO
2 is in competition with the volatility of their volatile components. The solubility of compounds alleviates as the temperature rises, although volatility increases. Although raising the temperature increases the extraction efficiency, many heat-sensitive compounds may break down or oxidize at higher extraction temperatures, losing their biological activity [
52,
75]. In order to prevent deterioration, the temperature condition for thermolabile compounds, especially for anthocyanins, must be adjusted between 35 and 60 °C [
11]. The effects of temperature and pressure are related to each other. The increase in temperature at constant pressure will decrease solvent density and its solvation power [
76]. The rise in temperature also will results in increasing solute vapor pressure, subsequently enhancing the solubility of a solute in the ScCO
2.
6.3. Effect of Co-Solvent/Modifier in ScCO2 Extraction
A co-solvent is described as an organic solvent that may dissolve in ScCO
2 at varying ratios and maintain a substantial degree of solvent power toward the targeted compounds [
77]. Due to its intrinsic polarity, scCO
2 is an ideal solvent for the extraction of nonpolar compounds, for example. However, pure CO
2 is seldom used to extract hydrophilic compounds [
78]. It is standard procedure to change the polarity of the ScCO
2 by adding small quantities of organic co-solvents in order to boost the solvating power towards the target molecules. Strong polarity-dependent interactions, such as hydrogen-bonding and dipole–dipole interactions, between co-solvents and bio-components, such as hydrogen-bonding and dipole–dipole interactions, greatly improve extraction yields [
79]. When finding the best co-solvent for a given extraction technique, the kind of material, targeted compounds, and preparatory tests should be considered [
72]. Ethanol and methanol are the organic solvents most typically utilized [
59]. According to the Food and Drug Administration (FDA) of the United States, ethanol is widely accepted as safe, making it the recommended co-solvent [
70]. Due to its toxicity, methanol is not employed in the manufacturing of food or oil, despite the fact that it is used in ScCO
2 processes on an analytical scale.
6.4. Effect of Particle Size in ScCO2 Extraction
Particle size is also a significant factor that contributes to the success of ScCO
2. According to Reverchon, et al. [
69], particle size affects to the mass transfer kinetics especially the diffusivity process and the contact of CO
2 to the soluble compounds. Higher extraction efficiencies and the mass transfer surface can be influenced by the small physical morphology of the sample matrix, resulting from shorter internal diffusion path lengths over which the extracted solutes move to the fluid phase [
80]. Generally, for natural products, the particle size varies from 0.25 to 1.80 mm; therefore, this issue must be evaluated case by case.
Another aspect that must be addressed is the particle’s form. As a result of the grinding process, the particles may be spherical, flaky, plate-like, or of various forms that will affect the diffusivity of the solvent in order to penetrate the raw material [
81]. This trend is supported by the observation that decreasing the particle size increased the diffusivity coefficient of
Momordica charantia extract in the range from 0.2 to 0.7 mm, thus the extraction yield increased [
82]. On the other hand, the smallest particle size (0.2 mm) gives the lowest diffusivity coefficients (0.72 × 10
−13 m/s
2). This is because a smaller particle size will cause compaction and channeling inside the bed. However, fine particle size may result in bed caking and the formation of the preferential CO
2 flow path, hence reducing the mass transfer. Therefore, it will reduce the anthocyanin recovery from roselle.
6.5. Effect of Flowrate in ScCO2 Extraction
CO
2 flow rate is another important factor that needs to be considered while conducting the experiment. Machmudah, et al. [
68] reported that enhancing the CO
2 flow rate not only minimized the residence time but also enhanced the number of particles in contact with the anthocyanins, thus enhancing the intermolecular interaction between CO
2 and the anthocyanins. Idham, et al. [
25] also stated that the mass transfer of the extraction, which may be subdivided into a solubility-controlled zone and a diffusion-controlled phase, is highly influenced by the flow rate. A reduced solvent flow enhances the effectiveness, especially in the solubility-controlled area, and reduces the total solvent mass used to extract a given quantity of anthocyanins as interest compounds. As predicted, the anthocyanin production was directly related to the quantity of CO
2 required for extraction in the solubility-controlled zone at lower pressures.
6.6. Effect of Extraction Time in ScCO2 Extraction
The extraction time relies on the types and conditions of raw material. It is also related to the other parameter such as pressure, temperature, size of particle and modifier. Generally, the extraction rate is increased as the time increased for short extraction time. However, towards the end of the extraction, the rate become slower due to transition to a diffusion controlled process from solubility controlled [
83]. In addition, shorter time usage will contributes to the less consumption of CO
2. Hossain, et al. [
84] stated that increasing of extraction time is not significant to enhance the palm kernel cake oil recovery. Salajegheh, et al. [
85] also stated that extraction efficiency of cocoa butter extract increases significantly up to 6 hours and then remain unchanged up to 8 h because the system reaches the equilibrium state.
7. Previous Studies of Anthocyanin Recovery from Roselle Using Scco2 Extraction
The presence of anthocyanin pigments in roselle is modest. Therefore, a particular extraction method is necessary to separate the dye-bearing components from their substrates as shown in
Table 5 [
74]. To obtain high yields and purity while keeping functional qualities such as color and bioactivity, the parameters of ScCO
2 extraction have to be defined. Anthocyanins were extracted from roselle calyces using ScCO
2 according to Idham, et al. [
11]. Using a three-factor design, three processing parameters, namely pressure, temperature, and co-solvent ratio (ethanol–water), were examined. The approach was used to simulate the extraction of anthocyanins and their color properties. At maximum anthocyanins, the optimal conditions were 27 MPa, 58 °C, and an 8.86% co-solvent ratio. The production of anthocyanins was 1197 mg/100 g of dried roselle. Temperature is a significant factor to enhance the total yield of anthocyanins and color stability.
Idham, et al. [
25] also discovered that the examination of the solubility yield and anthocyanin content of roselle extract at various temperatures of 50 to 70 °C, pressures of 8 to 12 MPa, and modifier ratios of 5 to 10%. This research demonstrated that a low temperature and high pressure at a constant modifier ratio enhanced the solubility of anthocyanins. Furthermore, the increasing temperature, pressure, and modifier ratio also increased the anthocyanin recovery.
Idham, et al. [
41] also discovered that ScCO
2 extraction was conducted at constant pressure and temperature, 10 MPa and 70 °C, respectively, using 75% (
v/
v) ethanol. The data indicates that 120 min was the optimal extraction duration for determining the flow rate. An increase in extraction time did not increase the anthocyanin recovery, but it would increase the production cost due to large CO
2 consumption during the extraction process. These findings indicate that a low flow rate of 4 mL/min, a smaller particle size of 200 < dp < 355 µm, and a modifier percentage ratio of 10% might increase anthocyanin concentration. These process parameters might increase the selectivity of anthocyanin extraction.
Idham, et al. [
86] also discovered that ScCO
2 extraction of the red color of roselle was conducted using ethanol at pressures of 8 and 12 MPa, temperatures of 50 and 70 °C, and modifier flow rates between 5 and 10%. The total flow rate of CO
2, modifier (6 mL/min), the ratio of the modifier (75% ethanol-25% water), the particle size utilized (dp < 350 µm), and the extraction time (70 min) were kept constant. The results indicated that the extraction yield was substantially impacted by the three primary influences examined in this research. The optimal working parameters for ScCO
2 extraction of red color extract were 70 °C, 8.90 MPa, and 9.49%, with a red extract yield of 26.73%.
The optimal particle size (212 to 710 µm) was identified by Peng, et al. [
87] to obtain the maximum total yield. Using the response surface methodology, the effects of two factors, pressure (20 to 30 MPa) and temperature (40 to 80 °C), on extraction yield were investigated. Based on the experimental results, the optimal particle size, pressure, and temperature were determined to be 300 µm, 27.5 MPa, and 50.8 °C, respectively. The greatest extraction yield estimated with the optimized parameters was 163.26 mg-extract/g-dried material. The most significant factor in enhancing the roselle extract is temperature due to the degradation of anthocyanins at high temperature.
The phenolic and flavonoid compounds were also recovered from roselle. Rizkiyah, et al. [
70] discovered that pressure, temperature, and flow rate were used as variables in roselle extraction with ScCO
2. The best conditions, as determined using the response surface methodology, were, 40 °C, 20 MPa, and 4.875 mL/min, producing 14.11% yield of red extract, 816.16 mg/L of TFC, and 935.48 mg/L of TPC, respectively. Pressure and temperature were significant factors that enhanced the roselle extract with ScCO
2.
8. Comparison of ScCO2 Extraction and Traditional Extractions
Conventional techniques for extracting anthocyanins from plant material are nonselective and produce pigment mixtures including considerable quantities of by-products, such as sugar alcohols, sugars, amino acids, organic acids, amino acids, and proteins. Some of these contaminants hasten the decomposition of anthocyanins during storage [
88]. Efficient separation techniques should optimize anthocyanin recovery with few degradations or changes to its natural form. Additionally, the extraction technique should not be too complicated, dangerous, time-consuming, or expensive. For the extraction of anthocyanins, using solvents such as ethanol, methanol, and acetone is more effective than using other solvents. However, solvents such as methanol/acetone may be hazardous to human health [
52].
Maceration, Soxhlet extraction, and hydro distillation are frequently employed to extract bioactive compounds and natural pigments from a range of natural sources. The extraction temperature affects the effectiveness of the traditional extraction procedures. Although traditional procedures are straightforward, affordable, and simple to use, they need vast quantities of hazardous organic solvents, lengthy extraction durations, and additional solvent clean-up processes as shown in
Table 6. Low purity and a lack of selectivity may lead to pigment degradation and unfavorable compound extraction as a result of the long process required by conventional procedures. Pigments have traditionally been extracted using organic solvents such as hexane, chloroform, isopropanol, acetone, and methanol. Due to the polar to nonpolar characteristics of the majority of pigments, mixtures of solvents such as n-hexane are used.
The high pressure required for the ScCO
2 extraction process results in high capital and operations costs due to little or no toxic solvent. It is highly efficient, suitable for heat-sensitive pigments, and has a quick mass transfer (
Table 6). ScCO
2 is a nonpolar solvent with a predilection for nonpolar or low-polarity compounds, such as carotenoids [
91]. Therefore, its applicability for the extraction of polar pigments, such as anthocyanins, is limited. In this situation, increasing its affinity needs the addition of a co-solvent to convert supercritical CO
2 from a nonpolar to a polar solvent. Higher pressure increases the solvation power of CO
2 (exhausted process) but reduces the selectivity of CO
2 for compounds of interest, particularly anthocyanins.
9. Future Perspective
Numerous aspects of natural colorants need more investigation, and the present goal for scientists is to extract natural pigments from diverse sources, such as food sector leftovers, utilizing ecologically acceptable procedures to achieve low production cost and safety. Natural plant dyes may be in short supply due to limited resources. Therefore, roselle as a natural product has drawn the interest of scientists since it is seen as a possible source for novel natural high-yield dyes with extremely wide potential. In this respect, researchers are working to identify and validate the safety of red natural pigments; however, regulatory approval for these products is costly and time-consuming. Therefore, only a few naturally occurring colorants that are commercially accessible, such as anthocyanins, are FDA-approved for use in foods and drinks. As was previously stated, the restricted stability and poor solubility of natural colors in the application medium limit their employment in the food and pharmaceutical sectors. Alternative formulation procedures based on ScCO2 improve the solubility of anthocyanins. In addition, research was conducted on co-pigments and encapsulating methods to improve their hyperchromic effect, stability, solubility, and bioavailability. Co-pigments have been used to enhance the color and stability of a material. In the textile business, the anthocyanins derived from roselle are good for eczema sufferers. Nonetheless, the use of naturally colored textiles for wound healing has not yet been examined, and more study is required. Furthermore, ethyl acetate (EAc) can be applied as a substitute to extract anthocyanins from roselle in future research.
10. Conclusions
According to earlier research, ScCO2 extraction is a viable alternative to conventional extraction techniques that adds value by recovering high-quality pigments in an environmentally friendly way. This method has been utilized to generate unique natural red colorants, which has led to the creation of innovative concepts for the largest range of applications for these substances. Due to its pressure-tenable dissolving power, easy recovery, high selectivity, and nontoxicity, it is regarded as a green method for extracting natural antimicrobial colorants from different sources. ScCO2 parameters depends on anthocyanin recovery including temperature, pressure, flowrate, particle size, extraction time, and modifier ratio. The best parameters for the ScCO2 extraction of anthocyanins from roselle also influences the color’s stability. In many sectors, natural colorants extracted with ScCO2 are regarded as safe compounds, especially those meant for human consumption, such as novel functional food additives and textile and pharmaceutical colors.
Author Contributions
Conceptualization, N.R.P.; methodology, A.H.A.A.; software, D.N.R.; validation, D.N.R.; writing—original draft preparation, D.N.R., N.R.P., I.I. (Irianto Irianto), S.R., E.Y. and I.I. (Ikhwani Ikhwani); writing—review and editing, A.H.A.A. and I.V.; visualization, N.R.P. supervision, M.A.C.Y. All authors have read and agreed to the published version of the manuscript.
Funding
The authors would like to acknowledge the UTM Professional Development Research University grant (R.J130000.7113.05E53) and Department of General Education, Faculty of Resilience, Rabdan Academy, Abu Dhabi, United Arab Emirates for supporting this work.
Institutional Review Board Statement
Not applicable.
Informed Consent Statement
Not applicable.
Data Availability Statement
Data available on request from the authors.
Conflicts of Interest
The authors declare no conflict of interest.
References
- Rizkiyah, D.N.; Putra, N.R.; Idham, Z.; Che Yunus, M.A.; Veza, I.; Harny, I.; Syahlani, N.; Abdul Aziz, A.H. Optimization of Red Pigment Anthocyanin Recovery from Hibiscus sabdariffa by Subcritical Water Extraction. Processes 2022, 10, 2635. [Google Scholar] [CrossRef]
- Iglesias, I.; Echeverría, G.; Lopez, M. Fruit color development, anthocyanin content, standard quality, volatile compound emissions and consumer acceptability of several ‘Fuji’apple strains. Sci. Hortic. 2012, 137, 138–147. [Google Scholar] [CrossRef]
- Grajeda-Iglesias, C.; Figueroa-Espinoza, M.C.; Barouh, N.; Baréa, B.; Fernandes, A.; de Freitas, V.; Salas, E. Isolation and characterization of anthocyanins from Hibiscus sabdariffa flowers. J. Nat. Prod. 2016, 79, 1709–1718. [Google Scholar] [CrossRef]
- Pragalyaashree, M.M.; Tiroutchelvame, D.; Sashikumar, S. Degradation kinetics of anthocyanin extracted from roselle calyces (Hibiscus sabdariffa). J. Appl. Pharm. Sci. 2018, 8, 57–63. [Google Scholar]
- Sinela, A.; Rawat, N.; Mertz, C.; Achir, N.; Fulcrand, H.; Dornier, M. Anthocyanins degradation during storage of Hibiscus sabdariffa extract and evolution of its degradation products. Food Chem. 2017, 214, 234–241. [Google Scholar] [CrossRef]
- Seabra, I.J.; Braga, M.E.; Batista, M.T.; de Sousa, H.C. Fractioned high pressure extraction of anthocyanins from elderberry (Sambucus nigra L.) pomace. Food Bioprocess Technol. 2010, 3, 674–683. [Google Scholar] [CrossRef] [Green Version]
- Riaz, M.; Zia-Ul-Haq, M.; Saad, B. Introduction to anthocyanins. In Anthocyanins and Human Health: Biomolecular and Therapeutic Aspects; Springer: Berlin/Heidelberg, Germany, 2016; pp. 21–33. [Google Scholar]
- Alappat, B.; Alappat, J. Anthocyanin pigments: Beyond aesthetics. Molecules 2020, 25, 5500. [Google Scholar] [CrossRef]
- Miguel, G.; Fontes, C.; Antunes, D.; Neves, A.; Martins, D. Anthocyanin concentration of “Assaria” pomegranate fruits during different cold storage conditions. J. Biomed. Biotechnol. 2004, 2004, 338. [Google Scholar] [CrossRef] [Green Version]
- Felgines, C.; Talavéra, S.; Gonthier, M.-P.; Texier, O.; Scalbert, A.; Lamaison, J.-L.; Rémésy, C. Strawberry anthocyanins are recovered in urine as glucuro-and sulfoconjugates in humans. J. Nutr. 2003, 133, 1296–1301. [Google Scholar] [CrossRef] [Green Version]
- Idham, Z.; Putra, N.R.; Aziz, A.H.A.; Zaini, A.S.; Rasidek, N.A.M.; Mili, N.; Yunus, M.A.C. Improvement of extraction and stability of anthocyanins, the natural red pigment from roselle calyces using supercritical carbon dioxide extraction. J. CO2 Util. 2022, 56, 101839. [Google Scholar] [CrossRef]
- Numan, M.; Bashir, S.; Mumtaz, R.; Tayyab, S.; Rehman, N.U.; Khan, A.L.; Shinwari, Z.K.; Al-Harrasi, A. Therapeutic applications of bacterial pigments: A review of current status and future opportunities. 3 Biotech 2018, 8, 207. [Google Scholar] [CrossRef]
- Shahid, M.; Mohammad, F. Recent advancements in natural dye applications: A review. J. Clean. Prod. 2013, 53, 310–331. [Google Scholar] [CrossRef]
- Faizal, A.N.M.; Putra, N.R.; Zaini, M.A.A. Insight into the adsorptive mechanisms of methyl violet and reactive orange from water—A short review. Part. Sci. Technol. 2022, 1–10. [Google Scholar] [CrossRef]
- Faizal, A.N.M.; Yunus, M.A.C.; Ali, A.; Zaini, M.A.A. Insights into kinetics and equilibrium of methylene blue adsorption onto β-cyclodextrin polymers. Int. J. Chem. React. Eng. 2022. [Google Scholar]
- Argun, M.E.; Argun, M.Ş.; Arslan, F.N.; Nas, B.; Ates, H.; Tongur, S.; Cakmakcı, O. Recovery of valuable compounds from orange processing wastes using supercritical carbon dioxide extraction. J. Clean. Prod. 2022, 375, 134169. [Google Scholar] [CrossRef]
- Putra, N.R.; Rizkiyah, D.N.; Machmudah, S.; Shalleh, L.M.; Che Yunus, M.A. Recovery and solubility of flavonoid and phenolic contents from Arachis Hypogea in supercritical carbon dioxide assisted by ethanol as cosolvent. J. Food Process. Preserv. 2020, 44, e14768. [Google Scholar] [CrossRef]
- Bodoira, R.; Rossi, Y.; Montenegro, M.; Maestri, D.; Velez, A. Extraction of antioxidant polyphenolic compounds from peanut skin using water-ethanol at high pressure and temperature conditions. J. Supercrit. Fluids 2017, 128, 57–65. [Google Scholar] [CrossRef]
- Mohd-Nasir, H.; Putra, N.R.; Chuo, S.C.; Daud, N.M.; Bakeri, N.; Ruslan, M.S.H.; Mohd-Setapar, S.H.; Ahmad, A.; Md Salleh, L. Optimization of the Supercritical Carbon Dioxide Extraction of Quercus infectoria Galls Extracts and Its Bioactivities. J. Food Process. Preserv. 2020, 45, e15156. [Google Scholar]
- Abdul Aziz, A.H.; Mohd Idrus, N.F.; Putra, N.R.; Awang, M.A.; Idham, Z.; Mamat, H.; Che Yunus, M.A. Solubility of Rosmarinic Acid in Supercritical Carbon Dioxide Extraction from Orthosiphon stamineus Leaves. ChemEngineering 2022, 6, 59. [Google Scholar] [CrossRef]
- dos Santos, L.C.; Bitencourt, R.G.; dos Santos, P.; de Tarso Vieira e Rosa, P.; Martínez, J. Solubility of passion fruit (Passiflora edulis Sims) seed oil in supercritical CO2. Fluid Phase Equilibria 2019, 493, 174–180. [Google Scholar] [CrossRef]
- Soh, S.H.; Jain, A.; Lee, L.Y.; Jayaraman, S. Optimized extraction of patchouli essential oil from Pogostemon cablin Benth. with supercritical carbon dioxide. J. Appl. Res. Med. Aromat. Plants 2020, 19, 100272. [Google Scholar]
- Zabihi, S.; Esmaeili-Faraj, S.H.; Borousan, F.; Hezave, A.Z.; Shirazian, S. Loxoprofen solubility in supercritical carbon dioxide: Experimental and modeling approaches. J. Chem. Eng. Data 2020, 65, 4613–4620. [Google Scholar] [CrossRef]
- Daud, N.M.; Putra, N.R.; Jamaludin, R.; Norodin, N.S.M.; Sarkawi, N.S.; Hamzah, M.H.S.; Nasir, H.M.; Zaidel, D.N.A.; Yunus, M.A.C.; Salleh, L.M. Valorisation of plant seed as natural bioactive compounds by various extraction methods: A review. Trends Food Sci. Technol. 2022, 119, 201–214. [Google Scholar] [CrossRef]
- Idham, Z.; Putra, N.R.; Nasir, H.; Yian, L.N.; Idrus, N.F.M.; Yunus, M.A.C. Extraction and Solubility Modeling of Anthocyanins Rich Extract from Hibiscus sabdariffa L. using Supercritical Carbon Dioxide. Malays. J. Fundam. Appl. Sci. 2021, 17, 720–730. [Google Scholar] [CrossRef]
- Khazaei, K.M.; Jafari, S.; Ghorbani, M.; Kakhki, A.H.; Sarfarazi, M. Optimization of anthocyanin extraction from saffron petals with response surface methodology. Food Anal. Methods 2016, 9, 1993–2001. [Google Scholar] [CrossRef]
- Le, X.T.; Huynh, M.T.; Pham, T.N.; Than, V.T.; Toan, T.Q.; Bach, L.G.; Trung, N.Q. Optimization of total anthocyanin content, stability and antioxidant evaluation of the anthocyanin extract from Vietnamese Carissa carandas L. fruits. Processes 2019, 7, 468. [Google Scholar] [CrossRef] [Green Version]
- Lee, J.; Durst, R.W.; Wrolstad, R.E. Determination of total monomeric anthocyanin pigment content of fruit juices, beverages, natural colorants, and wines by the pH differential method: Collaborative study. J. AOAC Int. 2005, 88, 1269–1278. [Google Scholar] [CrossRef] [Green Version]
- Pazir, F.; Koçak, E.; Turan, F.; Ova, G. Extraction of anthocyanins from grape pomace by using supercritical carbon dioxide. J. Food Process. Preserv. 2021, 45, e14950. [Google Scholar] [CrossRef]
- Silva, S.; Costa, E.; Calhau, C.; Morais, R.; Pintado, M. Anthocyanin extraction from plant tissues: A review. Crit. Rev. Food Sci. Nutr. 2017, 57, 3072–3083. [Google Scholar] [CrossRef]
- Mount, D.I.; Anderson-Carnahan, L. Methods for Aquatic Toxicity Identification Evaluations: Phase I: Toxicity Characterization Procedures; US Environmental Protection Agency, Environmental Research Laboratory: Newport, OR, USA, 1988; Volume 2. [Google Scholar]
- Moirangthem, K.; Ramakrishna, P.; Amer, M.H.; Tucker, G.A. Bioactivity and anthocyanin content of microwave-assisted subcritical water extracts of Manipur black rice (Chakhao) bran and straw. Future Foods 2021, 3, 100030. [Google Scholar] [CrossRef]
- Castro, T.A.; Leite, B.S.; Assunção, L.S.; de Jesus Freitas, T.; Colauto, N.B.; Linde, G.A.; Otero, D.M.; Machado, B.A.S.; Ferreira Ribeiro, C.D. Red tomato products as an alternative to reduce synthetic dyes in the food industry: A review. Molecules 2021, 26, 7125. [Google Scholar] [CrossRef]
- Maranhão, M.V.M.; Nóbrega, D.K.A.d.; Anunciação, C.E.C.; Maia, B.d.A.B.; Mariano, P.V.D. Reação alérgica ao corante azul patente em cirurgia de mama-relato de caso. Rev. Bras. Anestesiol. 2016, 66, 433–436. [Google Scholar] [CrossRef] [Green Version]
- Sayed, H.M.; Fouad, D.; Ataya, F.S.; Hassan, N.H.; Fahmy, M.A. The modifying effect of selenium and vitamins A, C, and E on the genotoxicity induced by sunset yellow in male mice. Mutat. Res. Genet. Toxicol. Environ. Mutagen. 2012, 744, 145–153. [Google Scholar] [CrossRef]
- Giri, A.; Das, S.; Talukder, G.; Sharma, A. Sister chromatid exchange and chromosome aberrations induced by curcumin and tartrazine on mammalian cells in vivo. Cytobios 1990, 62, 111–117. [Google Scholar] [PubMed]
- Mpountoukas, P.; Pantazaki, A.; Kostareli, E.; Christodoulou, P.; Kareli, D.; Poliliou, S.; Mourelatos, C.; Lambropoulou, V.; Lialiaris, T. Cytogenetic evaluation and DNA interaction studies of the food colorants amaranth, erythrosine and tartrazine. Food Chem. Toxicol. 2010, 48, 2934–2944. [Google Scholar] [CrossRef] [PubMed]
- Chequer, F.M.D.; de Paula Venâncio, V.; Bianchi, M.d.L.P.; Antunes, L.M.G. Genotoxic and mutagenic effects of erythrosine B, a xanthene food dye, on HepG2 cells. Food Chem. Toxicol. 2012, 50, 3447–3451. [Google Scholar] [CrossRef] [PubMed]
- Ozaki, A.; Kitano, M.; Itoh, N.; Kuroda, K.; Furusawa, N.; Masuda, T.; Yamaguchi, H. Mutagenicity and DNA-damaging activity of decomposed products of food colours under UV irradiation. Food Chem. Toxicol. 1998, 36, 811–817. [Google Scholar] [CrossRef]
- Ali, H.M.; Almagribi, W.; Al-Rashidi, M.N. Antiradical and reductant activities of anthocyanidins and anthocyanins, structure–activity relationship and synthesis. Food Chem. 2016, 194, 1275–1282. [Google Scholar] [CrossRef]
- Idham, Z.; Zaini, A.S.; Putra, N.R.; Rusli, N.M.; Mahat, N.S.; Yian, L.N.; Che Yunus, M.A. Effect of flow rate, particle size and modifier ratio on the supercritical fluid extraction of anthocyanins from Hibiscus sabdariffa (L). IOP Conf. Ser. Mater. Sci. Eng. 2020, 932, 012031. [Google Scholar] [CrossRef]
- Zannou, O.; Koca, I.; Aldawoud, T.M.; Galanakis, C.M. Recovery and stabilization of anthocyanins and phenolic antioxidants of roselle (Hibiscus sabdariffa L.) with hydrophilic deep eutectic solvents. Molecules 2020, 25, 3715. [Google Scholar] [CrossRef]
- Chumsri, P.; Sirichote, A.; Itharat, A. Studies on the optimum conditions for the extraction and concentration of roselle (Hibiscus sabdariffa Linn.) extract. Songklanakarin J. Sci. Technol. 2008, 30, 133–139. [Google Scholar]
- Yu, J.; Ahmedna, M.; Goktepe, I.; Dai, J. Peanut skin procyanidins: Composition and antioxidant activities as affected by processing. J. Food Compos. Anal. 2006, 19, 364–371. [Google Scholar] [CrossRef]
- Cinquanta, L.; Di Matteo, M.; Esti, M. Physical pre-treatment of plums (Prunus domestica). Part 2. Effect on the quality characteristics of different prune cultivars. Food Chem. 2002, 79, 233–238. [Google Scholar] [CrossRef]
- Blando, F.; Gerardi, C.; Nicoletti, I. Sour cherry (Prunus cerasus L.) anthocyanins as ingredients for functional foods. J. Biomed. Biotechnol. 2004, 2004, 253. [Google Scholar] [CrossRef] [Green Version]
- Bimakr, M.; Rahman, R.A.; Taip, F.S.; Ganjloo, A.; Salleh, L.M.; Selamat, J.; Hamid, A.; Zaidul, I. Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves. Food Bioprod. Process. 2011, 89, 67–72. [Google Scholar] [CrossRef]
- Miguel, G.; Dandlen, S.; Antunes, D.; Neves, A.; Martins, D. The effect of two methods of pomegranate (Punica granatum L.) juice extraction on quality during storage at 4 C. J. Biomed. Biotechnol. 2004, 2004, 332. [Google Scholar] [CrossRef] [Green Version]
- Putra, N.R.; Aziz, A.H.A.; Faizal, A.N.M.; Che Yunus, M.A. Methods and Potential in Valorization of Banana Peels Waste by Various Extraction Processes: In Review. Sustainability 2022, 14, 10571. [Google Scholar] [CrossRef]
- Alvi, T.; Asif, Z.; Khan, M.K.I. Clean label extraction of bioactive compounds from food waste through microwave-assisted extraction technique—A review. Food Biosci. 2022, 46, 101580. [Google Scholar] [CrossRef]
- Zaini, A.S.; Putra, N.R.; Idham, Z.; Mohd Faizal, A.N.; Che Yunus, M.A.; Mamat, H.; Abdul Aziz, A.H. Comparison of alliin recovery from Allium sativum L. using Soxhlet extraction and subcritical water extraction. ChemEngineering 2022, 6, 73. [Google Scholar] [CrossRef]
- Arumugham, T.; Rambabu, K.; Hasan, S.W.; Show, P.L.; Rinklebe, J.; Banat, F. Supercritical carbon dioxide extraction of plant phytochemicals for biological and environmental applications—A review. Chemosphere 2021, 271, 129525. [Google Scholar] [CrossRef]
- Belwal, T.; Ezzat, S.M.; Rastrelli, L.; Bhatt, I.D.; Daglia, M.; Baldi, A.; Devkota, H.P.; Orhan, I.E.; Patra, J.K.; Das, G. A critical analysis of extraction techniques used for botanicals: Trends, priorities, industrial uses and optimization strategies. TrAC Trends Anal. Chem. 2018, 100, 82–102. [Google Scholar] [CrossRef]
- Wu, H.-Y.; Yang, K.-M.; Chiang, P.-Y. Roselle anthocyanins: Antioxidant properties and stability to heat and pH. Molecules 2018, 23, 1357. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jackman, R.L.; Yada, R.Y.; Tung, M.A. A review: Separation and chemical properties of anthocyanins used for their qualitative and quantitative analysis. J. Food Biochem. 1987, 11, 279–308. [Google Scholar] [CrossRef]
- Miranda-Medina, A.; Hayward-Jones, P.M.; Carvajal-Zarrabal, O.; de Guevara, L.d.A.L.; Ramírez-Villagómez, Y.D.; Barradas-Dermitz, D.M.; Luna-Carrillo, G.; Aguilar-Uscanga, M.G. Optimization of Hibiscus sabdariffa L. (Roselle) anthocyanin aqueous-ethanol extraction parameters using response surface methodology. Sci. Study Res. Chem. Chem. Eng. Biotechnol. Food Ind. 2018, 19, 53–62. [Google Scholar]
- Lukmanto, S.; Roesdiyono, N.; Ju, Y.-H.; Indraswati, N.; Soetaredjo, F.E.; Ismadji, S. Supercritical CO2 extraction of phenolic compounds in Roselle (Hibiscus sabdariffa L.). Chem. Eng. Commun. 2013, 200, 1187–1196. [Google Scholar] [CrossRef] [Green Version]
- Liu, F.-C.; Tsai, H.-I.; Yu, H.-P. Organ-protective effects of red wine extract, resveratrol, in oxidative stress-mediated reperfusion injury. Oxidative Med. Cell. Longev. 2015, 2015, 568634. [Google Scholar] [CrossRef] [PubMed]
- Marcus, Y. Extraction by subcritical and supercritical water, methanol, ethanol and their mixtures. Separations 2018, 5, 4. [Google Scholar] [CrossRef] [Green Version]
- Abdul Aziz, A.H.; Putra, N.R.; Nian Yian, L.; Mohd Rasidek, N.A.; Che Yunus, M.A. Parametric and kinetic study of supercritical carbon dioxide extraction on sinensetin from Orthosiphon stamineus Benth. leaves. Sep. Sci. Technol. 2022, 57, 444–453. [Google Scholar] [CrossRef]
- Quispe-Fuentes, I.; Uribe, E.; López, J.; Contreras, D.; Poblete, J. A study of dried mandarin (Clementina orogrande) peel applying supercritical carbon dioxide using co-solvent: Influence on oil extraction, phenolic compounds, and antioxidant activity. J. Food Process. Preserv. 2022, 46, e16116. [Google Scholar] [CrossRef]
- Putra, N.R.; Rizkiyah, D.N.; Idham, Z.; Jumakir, J.; Waluyo, W.; Mohd Faizal, A.N.; Ahmad Zaini, M.A.; Che Yunus, M.A. A New solubility model for competing effects of three solvents: Water, ethanol, and supercritical carbon dioxide. Sep. Sci. Technol. 2022, 57, 2269–2275. [Google Scholar] [CrossRef]
- Redzuan, S.; Ho, C.Y.; Idham, Z.; Yusuf, S.; Putra, N.R.; Yunus, M.A.C.; Mansor, S.; Ruslan, M.S.H. Optimization of Anthocyanins Extracts from Roselle (Hibiscus sabdarifa) Petals Using Ultrasonic-Assisted Extraction Method. In Proceedings of the 3rd International Conference on Separation Technology, Johor, Malaysia, 15–16 August 2020; pp. 295–309. [Google Scholar]
- Abou Elmaaty, T.; Sayed-Ahmed, K.; Elsisi, H.; Magdi, M. Optimization of Extraction of Natural Antimicrobial Pigments Using Supercritical Fluids: A Review. Processes 2022, 10, 2111. [Google Scholar] [CrossRef]
- Uwineza, P.A.; Waśkiewicz, A. Recent advances in supercritical fluid extraction of natural bioactive compounds from natural plant materials. Molecules 2020, 25, 3847. [Google Scholar] [CrossRef] [PubMed]
- Budisa, N.; Schulze-Makuch, D. Supercritical carbon dioxide and its potential as a life-sustaining solvent in a planetary environment. Life 2014, 4, 331–340. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Chemat, F.; Vian, M.A.; Cravotto, G. Green extraction of natural products: Concept and principles. Int. J. Mol. Sci. 2012, 13, 8615–8627. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Machmudah, S.; Shotipruk, A.; Goto, M.; Sasaki, M.; Hirose, T. Extraction of astaxanthin from Haematococcus pluvialis using supercritical CO2 and ethanol as entrainer. Ind. Eng. Chem. Res. 2006, 45, 3652–3657. [Google Scholar] [CrossRef]
- Reverchon, E.; Donsi, G.; Sesti Osseo, L. Modeling of supercritical fluid extraction from herbaceous matrices. Ind. Eng. Chem. Res. 1993, 32, 2721–2726. [Google Scholar] [CrossRef]
- Rizkiyah, D.N.; Jusoh, W.M.S.W.; Idham, Z.; Putra, N.R.; Che Yunus, M.A. Investigation of Phenolic, Flavonoid and Antioxidant Recovery and Solubility from Roselle Using Supercritical Carbon Dioxide: Experimental and Modelling. J. Food Process. Preserv. 2022, 46, e16670. [Google Scholar] [CrossRef]
- Peng, W.L.; Mohd-Nasir, H.; Setapar, S.H.M.; Ahmad, A.; Lokhat, D. Optimization of process variables using response surface methodology for tocopherol extraction from Roselle seed oil by supercritical carbon dioxide. Ind. Crops Prod. 2020, 143, 111886. [Google Scholar] [CrossRef]
- Putra, N.R.; Rizkiyah, D.N.; Idham, Z.; Salleh, L.M.; Machmudah, S.; Yunus, M.A.C. Formulation and evaluation of a new semi-empirical model for solubility of plant extracts in supercritical carbon dioxide assisted by ethanol as co-solvent. Chem. Eng. Commun. 2021, 208, 1326–1334. [Google Scholar] [CrossRef]
- Cobb, B.F.; Kallenbach, J.; Hall, C.A.; Pryor, S.W. Optimizing the Supercritical Fluid Extraction of Lutein from Corn Gluten Meal. Food Bioprocess Technol. 2018, 11, 757–764. [Google Scholar] [CrossRef]
- Cockrell, C.J.; Dicks, O.; Wang, L.; Trachenko, K.; Soper, A.K.; Brazhkin, V.V.; Marinakis, S. Experimental and modeling evidence for structural crossover in supercritical CO2. Phys. Rev. E 2020, 101, 052109. [Google Scholar] [CrossRef] [PubMed]
- Arumugham, T.; AlYammahi, J.; Rambabu, K.; Hassan, S.W.; Banat, F. Supercritical CO2 pretreatment of date fruit biomass for enhanced recovery of fruit sugars. Sustain. Energy Technol. Assess. 2022, 52, 102231. [Google Scholar] [CrossRef]
- Suleiman, N.; Baharin, B.S.; Sarker, M.Z.I.; Mirhosseini, S.H.; Ali, M.H. Optimisation of squalene recovery from palm oil by-product using integrated ScCO2-pressure swing. J. Oil Palm Res. 2018, 30, 570–578. [Google Scholar] [CrossRef]
- Jiao, G. Extraction of anthocyanins from haskap berry pulp using supercritical carbon dioxide: Influence of co-solvent composition and pretreatment. Lwt 2018, 98, 237–244. [Google Scholar] [CrossRef]
- Da Porto, C.; Decorti, D.; Natolino, A. Water and ethanol as co-solvent in supercritical fluid extraction of proanthocyanidins from grape marc: A comparison and a proposal. J. Supercrit. Fluids 2014, 87, 1–8. [Google Scholar] [CrossRef]
- Pimentel-Moral, S.; Borrás-Linares, I.; Lozano-Sánchez, J.; Arráez-Román, D.; Martínez-Férez, A.; Segura-Carretero, A. Supercritical CO2 extraction of bioactive compounds from Hibiscus sabdariffa. J. Supercrit. Fluids 2019, 147, 213–221. [Google Scholar] [CrossRef]
- Taylor, L.T. Supercritical Fluid Extraction; Wiley-Interscience: Hoboken, NJ, USA, 1996; Volume 4. [Google Scholar]
- Gaspar, F.; Lu, T.; Santos, R.; Al-Duri, B. Modelling the extraction of essential oils with compressed carbon dioxide. J. Supercrit. Fluids 2003, 25, 247–260. [Google Scholar] [CrossRef]
- Aris, N.A.; Zaini, A.S.; Nasir, H.M.; Idham, Z.; Vellasamy, Y.; Yunus, M.A.C. Effect of particle size and co-extractant in Momordica charantia extract yield and diffusion coefficient using supercritical CO2. Malays. J. Fundam. Appl. Sci. 2018, 14, 368–373. [Google Scholar] [CrossRef] [Green Version]
- Zaidul, I.S.M.; Nik Norulaini, N.A.; Mohd Omar, A.K.; Smith, R.L. Supercritical carbon dioxide (SC-CO2) extraction of palm kernel oil from palm kernel. J. Food Eng. 2007, 79, 1007–1014. [Google Scholar] [CrossRef]
- Hossain, M.S.; Norulaini, N.A.N.; Naim, A.Y.A.; Zulkhairi, A.R.M.; Bennama, M.M.; Omar, A.K.M. Utilization of the supercritical carbon dioxide extraction technology for the production of deoiled palm kernel cake. J. CO2 Util. 2016, 16, 121–129. [Google Scholar] [CrossRef]
- Salajegheh, D.; Vaziri, A.; Bastani, D. Supercritical extraction of cocoa butter from cocoa seed, using pure carbon dioxide, carbon dioxide with ethanol as co-solvent and ethane. Middle East J. Sci. Res. 2013, 13, 1010–1015. [Google Scholar]
- Idham, Z.; Nasir, H.; Yunus, M.; Lee, N.; Wong, L.; Hassan, H.; Setapar, S. Optimisation of supercritical CO2 extraction of red colour from roselle (Hibiscus Sabdariffa Linn.) calyces. Chem. Eng. Trans. 2017, 56, 871–876. [Google Scholar]
- Peng, W.L.; Setapar, S.H.M.; Nasir, H.M. Process optimization of supercritical CO2 extraction of Roselle using response surface methodology. Malays. J. Fundam. Appl. Sci. 2020, 16, 30–33. [Google Scholar] [CrossRef]
- Zaini, A.; Putra, N.; Idham, Z.; Norodin, N.M.; Rasidek, N.M.; Yunus, M.C. Mini Review: Extraction of Allicin from Allium sativum using Subcritical Water Extraction. IOP Conf. Ser. Mater. Sci. Eng. 2020, 932, 012023. [Google Scholar] [CrossRef]
- Putra, N.R.; Aziz, A.H.A.; Yian, L.N.; Ramli, W.D.; Yunus, M.A.C. Optimization of supercritical carbon dioxide and co-solvent ethanol extraction of wasted peanut skin using response surface methodology. MATEC Web Conf. 2018, 156, 02005. [Google Scholar] [CrossRef] [Green Version]
- De Oliveira, M.S.; Silva, S.G.; da Cruz, J.N.; Ortiz, E.; da Costa, W.A.; Bezerra, F.W.F.; Cunha, V.; Cordeiro, R.; de Jesus Chaves Neto, A.; de Aguiar Andrade, E. Supercritical CO2 application in essential oil extraction. Ind. Appl. Green Solvents 2019, 2, 1–28. [Google Scholar]
- Putra, N.R.; Wibobo, A.G.; Machmudah, S.; Winardi, S. Recovery of valuable compounds from palm-pressed fiber by using supercritical CO2 assisted by ethanol: Modeling and optimization. Sep. Sci. Technol. 2020, 55, 3126–3139. [Google Scholar] [CrossRef]
| Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. |
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).