Antimicrobial, Antioxidant, and Antityrosinase Activities of Morina persica L. and Its Isolated Compounds
Abstract
:1. Introduction
2. Results
2.1. Isolation Results
2.1.1. Kaempferol-3-O-β-glucopyranoside (Astragalin) (MP-1)
2.1.2. Quercetin-3-O-rutinoside (Rutin) (MP-2)
2.2. Antimicrobial Activity Results
2.3. Total Phenolic Compound Quantification Results
2.4. DPPH Radical Scavenging Study Results
2.5. ABTS Radical Scavenger Study Results
2.6. Tyrosinase Inhibitor Activity Results
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Extraction Studies
4.3. Isolation Studies and Structure Determination
4.4. Antimicrobial Studies
4.5. Antioxidant Studies
4.5.1. Determination of Total Phenolic Compound Quantity
4.5.2. DPPH Radical Scavenger Capacity Determination
4.5.3. ABTS+ Radical Scavenger Capacity Determination
4.6. Determination of Tyrosinase Enzyme Inhibition
- A: Phosphate buffer and enzyme
- B: Phosphate buffer
- C: Phosphate buffer, enzyme, and sample
- D: Phosphate buffer and sample
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Escherichia coli (ATCC 25922) | Klebsiella pneumoniae (ATCC 700603) | Pseudomonas aeruginosa (ATCC 9027) | Staphylococcus aureus (ATCC 29213) | Enterococcus faecalis (ATCC 29212) | |
---|---|---|---|---|---|
Dichloromethane | 64 | 16 | 512 | 1 | 2 |
n-Hexane | 32 | 64 | 1024 | 1 | 4 |
Ethyl acetate | 32 | 64 | 1024 | 8 | 64 |
Aqueous | 128 | 256 | 1024 | 4 | 32 |
Methanol | 4 | 256 | 1024 | 4 | 8 |
MP-1 | 32 | 1024 | 1024 | 1 | 4 |
MP-2 | 128 | 1024 | 1024 | 1 | 1 |
Sulfamerazine | 1600 | 1600 | 1600 | 6.25 | 3.12 |
Extract | Total Phenolic Compound (μg GAE/mg Extract) |
---|---|
Methanol | 49.29 ± 0.105 |
n-Hexane | 22.24 ± 0.080 |
Dichloromethane | 53.23 ± 0.152 |
Ethyl acetate | 169.24 ± 0.229 |
Aqueous | 46.71 ± 0.059 |
% Inhibition (50 μg/mL) | |
---|---|
Methanol | 11.80 ± 0.021 |
n-Hexane | 5.50 ± 0.003 |
Dichloromethane | 10.65 ± 0.041 |
Ethyl acetate | 38.80 ± 0.136 |
Aqueous | 10.25 ± 0.066 |
MP-1 | 43.88 ± 0.106 |
MP-2 | 19.00 ± 0.023 |
Trolox | 93.38 ± 0.209 |
% Inhibition (50 μg/mL) | |
---|---|
Methanol | 40.92 ± 0.198 |
n-Hexane | 15.99 ± 0.034 |
Dichloromethane | 28.70 ± 0.148 |
Ethyl acetate | 98.92 ± 0.341 |
Aqueous | 42.88 ± 0.127 |
MP-1 | 93.10 ± 0.152 |
MP-2 | 68.59 ± 0.211 |
Trolox | 98.71 ± 0.301 |
% Inhibition (200 μg/mL) | |
---|---|
Methanol | 57.14 ± 0.184 |
n-Hexane | 31.03 ± 0.197 |
Dichloromethane | 51.72 ± 0.203 |
Ethyl acetate | 20.35 ± 0.136 |
Aqueous | 17.24 ± 0.105 |
Kojic acid | 90.48 ± 0.312 |
IC50 (μg/mL) | |
---|---|
MP-1 | 297.9 ± 1.2 |
MP-2 | 115.2 ± 0.8 |
Kojic acid | 14.6 ± 0.5 |
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Özgen, R.; Sezen Karaoğlan, E.; Sevindik, H.G.; Hancı, H.; Kazaz, C. Antimicrobial, Antioxidant, and Antityrosinase Activities of Morina persica L. and Its Isolated Compounds. Molecules 2024, 29, 3017. https://doi.org/10.3390/molecules29133017
Özgen R, Sezen Karaoğlan E, Sevindik HG, Hancı H, Kazaz C. Antimicrobial, Antioxidant, and Antityrosinase Activities of Morina persica L. and Its Isolated Compounds. Molecules. 2024; 29(13):3017. https://doi.org/10.3390/molecules29133017
Chicago/Turabian StyleÖzgen, Rıdvan, Esen Sezen Karaoğlan, Handan Gökben Sevindik, Hayrunisa Hancı, and Cavit Kazaz. 2024. "Antimicrobial, Antioxidant, and Antityrosinase Activities of Morina persica L. and Its Isolated Compounds" Molecules 29, no. 13: 3017. https://doi.org/10.3390/molecules29133017