Metabolite Variations during the First Weeks of Growth of Immature Citrus sinensis and Citrus reticulata by Untargeted Liquid Chromatography–Mass Spectrometry/Mass Spectrometry Metabolomics
Abstract
:1. Introduction
2. Results and Discussion
2.1. Key Metabolites Responsible for Differentiating Immature Varieties
2.2. Evolution of Major Metabolites in Growing Fruits during the First Weeks of Growth
3. Materials and Methods
3.1. Chemicals
3.2. Fruit Samples and Sample Preparation
3.3. Instrumentation
3.4. Data Processing and Data Analysis
3.4.1. Data Processing
3.4.2. Statistical Analysis
3.4.3. Metabolite Identifications
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chemical Family | Number of Up-Regulated Compounds in the ‘Fremont’ Mandarin | Number of Up-Regulated Compounds in the Oranges |
---|---|---|
Amino acids | 0 | 4 |
Amines | 4 | 3 |
Peptides | 0 | 1 |
Amino sugars | 0 | 4 |
Sugars | 0 | 2 |
Lipids | 1 | 1 |
Polyphenols | 0 | 6 |
Polymethoxyflavones | 11 | 10 |
Polymethoxyflavanones | 0 | 1 |
Flavones | 2 | 5 |
Organic phosphorous | 0 | 1 |
Chemical Family | Number of Compounds with an Intensity Increase | Number of Compounds with an Intensity Decrease |
---|---|---|
Organic acids | 1 | 0 |
Amino acids | 1 | 0 |
Peptides | 0 | 1 |
Amines | 5 | 11 |
Amino sugars | 10 | 1 |
Sugars | 4 | 2 |
Lipids | 0 | 3 |
Polyphenols | 3 | 3 |
Limonoids | 3 | 0 |
Polymethoxyflavones | 3 | 10 |
Flavones | 3 | 0 |
Flavanones | 2 | 0 |
Organic phosphorus | 5 | 0 |
Variety | Citrus sinensis (L.) Obs. | Citrus sinensis (L.) Obs. | Citrus sinensis (L.) Obs. | Citrus reticulata Ten. |
---|---|---|---|---|
Cultivar | Valencia campbell | Valencia Late | Navelate | Fremont |
SRA 17 | SRA 246 | SRA 307 | SRA 147 | |
Rootstock | Poncirus trifoliata | Poncirus trifoliata | Poncirus trifoliata | Poncirus trifoliata |
Plot location | Ala H1-4 | Ala H1-4 | Ala O17-20 | B3 G1-4 |
Time (min) | Mobile Phase B (%) |
---|---|
0 | 0 |
2 | 0 |
4 | 40 |
10 | 100 |
14 | 100 |
14.5 | 0 |
16 | 0 |
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Deschamps, E.; Durand-Hulak, M.; Castagnos, D.; Hubert-Roux, M.; Schmitz, I.; Froelicher, Y.; Afonso, C. Metabolite Variations during the First Weeks of Growth of Immature Citrus sinensis and Citrus reticulata by Untargeted Liquid Chromatography–Mass Spectrometry/Mass Spectrometry Metabolomics. Molecules 2024, 29, 3718. https://doi.org/10.3390/molecules29163718
Deschamps E, Durand-Hulak M, Castagnos D, Hubert-Roux M, Schmitz I, Froelicher Y, Afonso C. Metabolite Variations during the First Weeks of Growth of Immature Citrus sinensis and Citrus reticulata by Untargeted Liquid Chromatography–Mass Spectrometry/Mass Spectrometry Metabolomics. Molecules. 2024; 29(16):3718. https://doi.org/10.3390/molecules29163718
Chicago/Turabian StyleDeschamps, Estelle, Marie Durand-Hulak, Denis Castagnos, Marie Hubert-Roux, Isabelle Schmitz, Yann Froelicher, and Carlos Afonso. 2024. "Metabolite Variations during the First Weeks of Growth of Immature Citrus sinensis and Citrus reticulata by Untargeted Liquid Chromatography–Mass Spectrometry/Mass Spectrometry Metabolomics" Molecules 29, no. 16: 3718. https://doi.org/10.3390/molecules29163718
APA StyleDeschamps, E., Durand-Hulak, M., Castagnos, D., Hubert-Roux, M., Schmitz, I., Froelicher, Y., & Afonso, C. (2024). Metabolite Variations during the First Weeks of Growth of Immature Citrus sinensis and Citrus reticulata by Untargeted Liquid Chromatography–Mass Spectrometry/Mass Spectrometry Metabolomics. Molecules, 29(16), 3718. https://doi.org/10.3390/molecules29163718