Desmodium intortum (Mill.) Urb. Protein Isolate Aggregates as Pickering Stabilizers: Physicochemical Characteristics and Emulsifying Properties
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of DIPI Aggregates
2.2. Influence of Ionic Strength and pH on the Characteristics of DIPI Aggregates
2.2.1. Size, Zeta Potential, and Surface Hydrophobicity: Modulation of Ionic Strength
2.2.2. Size, Zeta Potential, and Surface Hydrophobicity: Modulation of pH
2.2.3. Contact Angle and SEM
2.3. Characteristics of Pickering Emulsions Stabilized by DIPI
2.3.1. Influence of DIPI Concentration and Oil Fraction on Emulsion Rheological Properties
2.3.2. Influence of DIPI Concentration on Emulsion Droplet Size and Microstructure
2.3.3. Influence of Oil Fraction on Emulsion Droplet Size and Microstructure
2.3.4. Influence of DIPI Concentration and Oil Fraction on Emulsion Creaming Index
2.3.5. Confocal Laser Scanning Microscopy Analysis of DIPIPE
3. Materials and Methods
3.1. Materials
3.2. Preparation of DIPI Aggregates
3.3. Characterization of DIPI Aggregate
3.3.1. Size Distribution, Particle Size, and Zeta Potential
3.3.2. Surface Hydrophobicity (H0)
3.3.3. Contact Angle Measurements
3.3.4. Scanning Electron Microscopy (SEM)
3.4. Characterization of DIPIPE
3.4.1. Preparation of DIPIPE
3.4.2. Rheological Properties
3.4.3. Droplet Size Distribution and Volume-Averaged Droplet Size (D4,3)
3.4.4. Microstructure
3.4.5. Creaming Index (CI)
3.4.6. Confocal Laser Scanning Microscopy (CLSM)
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Tang, X.; Chang, H.; Yao, G.; Chen, J.; Dong, R. Desmodium intortum (Mill.) Urb. Protein Isolate Aggregates as Pickering Stabilizers: Physicochemical Characteristics and Emulsifying Properties. Molecules 2024, 29, 3923. https://doi.org/10.3390/molecules29163923
Tang X, Chang H, Yao G, Chen J, Dong R. Desmodium intortum (Mill.) Urb. Protein Isolate Aggregates as Pickering Stabilizers: Physicochemical Characteristics and Emulsifying Properties. Molecules. 2024; 29(16):3923. https://doi.org/10.3390/molecules29163923
Chicago/Turabian StyleTang, Xuemei, Hui Chang, Guanglong Yao, Jian Chen, and Rongshu Dong. 2024. "Desmodium intortum (Mill.) Urb. Protein Isolate Aggregates as Pickering Stabilizers: Physicochemical Characteristics and Emulsifying Properties" Molecules 29, no. 16: 3923. https://doi.org/10.3390/molecules29163923