Calcium Citrate Amount and Gelatine Source Impact on Hydroxyapatite Formation in Bone Regeneration Material in Simulated Body Fluid
Abstract
:1. Introduction
2. Results
2.1. Conversion of Different Amounts of Calcium Citrate Powder Immersed in Simulated Body Fluid
2.2. Conversion of Octacalcium Phosphate/Calcium Citrate/Porcine Gelatine Composite Samples Produced in 96- and 24-Well Plates Immersed in Simulated Body Fluid
2.3. Conversion of Octacalcium Phosphate/Calcium Citrate/Gelatine Composite Samples with Different Gelatine Sources Immersed in Simulated Body Fluid
3. Discussion
4. Materials and Methods
4.1. Sample Preparation
4.1.1. Synthesis of Octacalcium Phosphate
4.1.2. Synthesis of Octacalcium Phosphate/Calcium Citrate/Gelatine
4.2. Soaking in Simulated Body Fluid
4.3. Characterisation
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Order | Reagent | Amount |
---|---|---|
1 | NaCl | 7.996 g |
2 | NaHCO3 | 0.350 g |
3 | KCl | 0.224 g |
4 | K2HPO4·3H2O | 0.228 g |
5 | MgCl2·6H2O | 0.305 g |
6 | 1.0 mol/L HCl | 40 mL |
7 | CaCl2 | 0.278 g |
8 | Na2SO4 | 0.071 g |
9 | (CH2OH)3CNH2 | 6.057 g |
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Wang, Y.; Yokoi, T.; Shimabukuro, M.; Kawashita, M. Calcium Citrate Amount and Gelatine Source Impact on Hydroxyapatite Formation in Bone Regeneration Material in Simulated Body Fluid. Molecules 2024, 29, 3925. https://doi.org/10.3390/molecules29163925
Wang Y, Yokoi T, Shimabukuro M, Kawashita M. Calcium Citrate Amount and Gelatine Source Impact on Hydroxyapatite Formation in Bone Regeneration Material in Simulated Body Fluid. Molecules. 2024; 29(16):3925. https://doi.org/10.3390/molecules29163925
Chicago/Turabian StyleWang, Yuejun, Taishi Yokoi, Masaya Shimabukuro, and Masakazu Kawashita. 2024. "Calcium Citrate Amount and Gelatine Source Impact on Hydroxyapatite Formation in Bone Regeneration Material in Simulated Body Fluid" Molecules 29, no. 16: 3925. https://doi.org/10.3390/molecules29163925