Fast Screening of Tyrosinase Inhibitors in Coreopsis tinctoria Nutt. by Ligand Fishing Based on Paper-Immobilized Tyrosinase
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of CP@DA@TYR
2.2. Selection of the Buffer System
2.3. Identification of the TYR Ligands
2.4. Quantification of the TYR Ligands
2.5. TYR Inhibitory Activity
2.6. Enzyme Kinetic Study
2.7. Molecular Docking
2.8. Inhibition of the Intracellular TYR Activity and Melanin Synthesis
3. Materials and Methods
3.1. Materials and Reagents
3.2. Immobilization of TYR on Cellulose Chromatography Paper and Characterization
3.3. Ligand Fishing of the TYR Inhibitors
3.4. Selection of the Buffer System
3.5. Extraction and Isolation of the TYR Inhibitors
3.6. Structural Identification of the TYR Ligands by UPLC-MS
3.7. Quantification of the TYR Ligands
3.8. TYR Inhibition Assay
3.9. Enzymatic Kinetic Study
3.10. Molecular Docking
3.11. In Vitro TYR Inhibition Activity
3.11.1. Cell Culture
3.11.2. Cytotoxicity Assay
3.11.3. Intracellular TYR Inhibition Activity Assay
3.11.4. Quantification of Intracellular Melanin Content
3.12. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Ablat, A.; Li, M.-J.; Zhai, X.-R.; Wang, Y.; Bai, X.-L.; Shu, P.; Liao, X. Fast Screening of Tyrosinase Inhibitors in Coreopsis tinctoria Nutt. by Ligand Fishing Based on Paper-Immobilized Tyrosinase. Molecules 2024, 29, 4018. https://doi.org/10.3390/molecules29174018
Ablat A, Li M-J, Zhai X-R, Wang Y, Bai X-L, Shu P, Liao X. Fast Screening of Tyrosinase Inhibitors in Coreopsis tinctoria Nutt. by Ligand Fishing Based on Paper-Immobilized Tyrosinase. Molecules. 2024; 29(17):4018. https://doi.org/10.3390/molecules29174018
Chicago/Turabian StyleAblat, Ayzohra, Ming-Jie Li, Xiao-Rui Zhai, Yuan Wang, Xiao-Lin Bai, Peng Shu, and Xun Liao. 2024. "Fast Screening of Tyrosinase Inhibitors in Coreopsis tinctoria Nutt. by Ligand Fishing Based on Paper-Immobilized Tyrosinase" Molecules 29, no. 17: 4018. https://doi.org/10.3390/molecules29174018