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Review

The ATTO 565 Dye and Its Applications in Microscopy

by
Yuheng Wu
and
René M. Williams
*
Molecular Photonics Group, Van ’t Hoff Institute for Molecular Sciences (HIMS), Universiteit van Amsterdam, Science Park 904, 1098 XH Amsterdam, The Netherlands
*
Author to whom correspondence should be addressed.
Molecules 2024, 29(17), 4243; https://doi.org/10.3390/molecules29174243
Submission received: 10 July 2024 / Revised: 28 August 2024 / Accepted: 29 August 2024 / Published: 6 September 2024
(This article belongs to the Special Issue Advances in Functional Organic Dye Chemistry)

Abstract

ATTO 565, a Rhodamine-type dye, has garnered significant attention due to its remarkable optical properties, such as a high fluorescence quantum yield, and the fact that it is a relatively stable structure and has low biotoxicity. ATTO 565 has found extensive applications in combination with microscopy technology. In this review, the chemical and optical properties of ATTO 565 are introduced, along with the principles behind them. The functionality of ATTO 565 in confocal microscopy, stimulated emission depletion (STED) microscopy, single-molecule tracking (SMT) techniques, two-photon excitation–stimulated emission depletion microscopy (TPE-STED) and fluorescence correlation spectroscopy (FCS) is discussed. These studies demonstrate that ATTO 565 plays a crucial role in areas such as biological imaging and single-molecule localization, thus warranting further in-depth investigations. Finally, we present some prospects and concepts for the future applications of ATTO 565 in the fields of biocompatibility and metal ion detection. This review does not include theoretical calculations for the ATTO 565 molecule.
Keywords: high-resolution microscopy; fluorescent Rhodamine dye; stimulated emission depletion microscopy; fluorescent labeling; fluorescence correlation spectroscopy; 3D single-molecule tracking high-resolution microscopy; fluorescent Rhodamine dye; stimulated emission depletion microscopy; fluorescent labeling; fluorescence correlation spectroscopy; 3D single-molecule tracking

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MDPI and ACS Style

Wu, Y.; Williams, R.M. The ATTO 565 Dye and Its Applications in Microscopy. Molecules 2024, 29, 4243. https://doi.org/10.3390/molecules29174243

AMA Style

Wu Y, Williams RM. The ATTO 565 Dye and Its Applications in Microscopy. Molecules. 2024; 29(17):4243. https://doi.org/10.3390/molecules29174243

Chicago/Turabian Style

Wu, Yuheng, and René M. Williams. 2024. "The ATTO 565 Dye and Its Applications in Microscopy" Molecules 29, no. 17: 4243. https://doi.org/10.3390/molecules29174243

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