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Open AccessArticle
Efficient Production of Some Bioactive Depsides and Simple Phenolic Acids by Microshoots of Aronia × Prunifolia (Purple Aronia) Agitated Cultures as the Result of Feeding Strategy with Four Different Biogenetic Precursors
by
Paweł Kubica
Paweł Kubica †,
Agnieszka Szopa
Agnieszka Szopa *,†,
Adam Setkiewicz
Adam Setkiewicz † and
Halina Ekiert
Halina Ekiert †
Department of Medicinal Plant and Mushroom Biotechnology, Jagiellonian University, Medical College, 30-688 Kraków, Poland
*
Author to whom correspondence should be addressed.
†
(until 1 June 2024—Chair and Department of Pharmaceutical Botany).
Molecules 2024, 29(19), 4622; https://doi.org/10.3390/molecules29194622 (registering DOI)
Submission received: 30 July 2024
/
Revised: 17 September 2024
/
Accepted: 25 September 2024
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Published: 29 September 2024
Abstract
A precursor feeding strategy was used for the first time in agitated microshoot cultures of Aronia × prunifolia. This strategy involved the addition of biogenetic precursors of simple phenolic acids (phenylalanine, cinnamic acid, and benzoic acid) and depsides (caffeic acid) into the culture media, with an assessment of its effect on the production of these bioactive compounds. The in vitro cultures were maintained in Murashige–Skoog medium (1 mg/L BAP and 1 mg/L NAA). Precursors at five concentrations (0.1, 0.5, 1.0, 5.0, and 10.0 mmol/L) were fed into the medium at the time of culture initiation (point “0”) and independently on the 10th day of growth cycles. The contents of 23 compounds were determined in methanolic extracts of biomass collected after 20 days of growth cycles using an HPLC method. All extracts contained the same four depsides (chlorogenic, neochlorogenic, rosmarinic, and cryptochlorogenic acids) and the same four simple phenolic acids (protocatechuic, vanillic, caffeic, and syringic acids). Chlorogenic and neochlorogenic acids were the predominant compounds in all extracts (max. 388.39 and 263.54 mg/100 g d.w.). The maximal total contents of all compounds were confirmed after feeding with cinnamic acid (5 mmol/L, point “0”) and caffeic acid (10 mmol/L, point “0”), which caused a 2.68-fold and 2.49-fold increase in the contents of the estimated compounds vs. control cultures (603.03 and 558.48 mg/100 g d.w., respectively). The obtained results documented the efficacy of the precursor feeding strategy in enhancing the production of bioactive compounds in agitated cultures of A. × prunifolia and suggest a potential practical application value.
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MDPI and ACS Style
Kubica, P.; Szopa, A.; Setkiewicz, A.; Ekiert, H.
Efficient Production of Some Bioactive Depsides and Simple Phenolic Acids by Microshoots of Aronia × Prunifolia (Purple Aronia) Agitated Cultures as the Result of Feeding Strategy with Four Different Biogenetic Precursors. Molecules 2024, 29, 4622.
https://doi.org/10.3390/molecules29194622
AMA Style
Kubica P, Szopa A, Setkiewicz A, Ekiert H.
Efficient Production of Some Bioactive Depsides and Simple Phenolic Acids by Microshoots of Aronia × Prunifolia (Purple Aronia) Agitated Cultures as the Result of Feeding Strategy with Four Different Biogenetic Precursors. Molecules. 2024; 29(19):4622.
https://doi.org/10.3390/molecules29194622
Chicago/Turabian Style
Kubica, Paweł, Agnieszka Szopa, Adam Setkiewicz, and Halina Ekiert.
2024. "Efficient Production of Some Bioactive Depsides and Simple Phenolic Acids by Microshoots of Aronia × Prunifolia (Purple Aronia) Agitated Cultures as the Result of Feeding Strategy with Four Different Biogenetic Precursors" Molecules 29, no. 19: 4622.
https://doi.org/10.3390/molecules29194622
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