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Review

Research Progress on the Mechanisms of Protocatechuic Acid in the Treatment of Cognitive Impairment

by
Shuzhi Liang
1,
Zhongmin Zhao
1,
Leilei Liu
1,
Yan Zhang
1,2,* and
Xijian Liu
1,*
1
College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan 250300, China
2
The Youth Research and Innovation Team of TCM for the Prevention and Treatment of Cardiovascular and Cerebrovascular Diseases, Shandong University of Traditional Chinese Medicine, Jinan 250300, China
*
Authors to whom correspondence should be addressed.
Molecules 2024, 29(19), 4724; https://doi.org/10.3390/molecules29194724 (registering DOI)
Submission received: 11 August 2024 / Revised: 25 September 2024 / Accepted: 3 October 2024 / Published: 6 October 2024
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Abstract

:
Cognitive impairment (CI) is a type of mental health disorder that mainly affects cognitive abilities, such as learning, memory, perception, and problem-solving. Currently, in clinical practice, the treatment of cognitive impairment mainly focuses on the application of cholinesterase inhibitors and NMDA receptor antagonists; however, there is no specific and effective drug yet. Procatechuic acid (PCA) possesses various functions, including antibacterial, antiasthmatic, and expectorant effects. In recent years, it has received growing attention in the cognitive domain. Therefore, by summarizing the mechanisms of action of procatechuic acid in the treatment of cognitive impairment in this paper, it is found that procatechuic acid has multiple effects, such as regulating the expression of neuroprotective factors, inhibiting cell apoptosis, promoting the autophagy-lysosome pathway, suppressing oxidative stress damage, inhibiting inflammatory responses, improving synaptic plasticity dysfunction, inhibiting Aβ deposition, reducing APP hydrolysis, enhancing the cholinergic system, and inhibiting the excitotoxicity of neuronal cells. The involved signaling pathways include activating Pi3K-akt-mTor and inhibiting JNK, P38 MAPK, P38-ERK-JNK, SIRT1, and NF-κB/p53, etc. This paper aims to present the latest progress in research on procatechuic acid, including aspects such as its chemical properties, sources, pharmacokinetics, mechanisms for treating neurodegenerative diseases.

1. Introduction

Cognitive impairment (CI), a primary mental health disorder that mainly affects cognitive abilities (including learning, memory, perception, and problem-solving), represents the earliest clinically detectable stage of dementia. It is frequently observed in disease complications such as stroke, cerebral infarction, and diabetes. The main manifestations are the progressive weakening of memory and the gradual deterioration of cognitive functions [1]. With the intensification of aging, the prevalence of mild cognitive impairment has been increasing annually. CI patients require lifelong medication and need assistance in daily life, imposing a heavy medical burden on both patients and their families. The pathological characteristics of cognitive impairment can be manifested as atrophy of the medial temporal lobe and hippocampus as well as varying degrees of sclerosis, β-amyloid plaques, neurofibrillary tangles, granule cell degeneration, and apoptosis of hippocampal neurons, etc. [2]. Currently, in clinical treatment, cholinesterase inhibitors such as donepezil are commonly used to inhibit AChE activity, slowing the decomposition of acetylcholine in the synaptic cleft and thereby increasing the content of ACh to improve the cognitive function of patients with Alzheimer’s disease. NMDA receptor antagonists like memantine are employed to regulate the development of neuronal dendrites [3]. However, the former can lead to adverse reactions such as diarrhea, muscle cramps, fatigue, nausea, vomiting, and insomnia, while the latter can cause respiratory depression. Hence, how to effectively prevent and treat CI is a current research focus. Procatechuic acid has multiple functions such as antibacterial, antiasthmatic, and expectorant effects, and has gradually gained attention in the cognitive field in recent years [4].

2. Chemical Properties of Protocatechuic Acid

Protocatechuic acid (PCA), also known as 3,4-dihydroxybenzoic acid, is a natural phenolic acid with the Molecule ID of MOL000105. It is a white-to-brown crystalline powder that undergoes color change in air. The density is 1.54 g/cm3, the molecular formula is (HO)2C6H3COOH, the molecular weight is 154.12, and the melting point is approximately 200 °C. The molecular structure is depicted in Figure 1. It is soluble in ethanol and ether. Currently, research on the functions of PCA mainly focuses on its antioxidant activity, inhibition of tumor cell proliferation [5], and anti-diabetes [6]. In recent years, PCA has been recognized as having neuroprotective and cognitive-enhancing effects [7]. This article reviews the literature on the treatment of cognitive impairment with PCA discovered in the past five years, providing the latest comprehensive evaluation for future clinical research on PCA for neurodegenerative diseases.

3. Sources of Protocatechuic Acid

3.1. Direct Sources

PCA is present not only in berries and red wine but also in traditional Chinese medicines such as rose [8], wolfberry [9], schizandra [10], pueraria root [11], scutellaria barbata [12], eucommia ulmoides [13], acanthopanax senticosus [14], smilax glabra [15], prunella vulgaris [16], solanum nigrum [17], hedyotis diffusa [18], plantago seed [19], dried tangerine peel [20], euphorbia pekinensis [21], cynomorium songaricum [22], rheum officinale [23], salvia miltiorrhiza [24], phellinus igniarius [25], ephedra [26], sterculia lychnophora [27], and cinnamon [28]. The content of PCA varies in different traditional Chinese medicines, see Table 1. The factors causing the content differences include temperature, pressure, origin of medicinal materials, and the composition and concentration of extraction solvents [8,9,10,11,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28].

3.2. Indirect Sources

A small portion of phenolic acids and aldehydes—such as anthocyanin [29], quercetin [30], protocatechuic aldehyde [31], and isoquercitrin [32]—is absorbed through the small intestine, while the majority reaches the colon intact and undergoes intestinal fermentation to be transformed into metabolites such as PCA. Moreover, the transformation conditions are associated with pH value and intestinal flora. It has been confirmed that PCA is transformed in neutral and alkaline fluids and within the intestinal flora. For instance, anthocyanin is stable in acidic environments but its b-ring cleavage occurs under neutral and alkaline pH conditions, decomposing into PCA [29]; therefore, quercetin [30], protocatechuic aldehyde [31], and isoquercitrin [32] can be decomposed by the intestinal microbiota in the human body, and the resulting metabolites include PCA, as depicted in Figure 2.

3.3. Pharmacokinetics

Oral PCA is absorbed through the stomach and reaches the liver via the portal vein, where it undergoes metabolism to form active metabolites. The primary metabolic pathway involves the combination of hydroxyl groups on protocatechuic acid with sulfate and glucuronic acid to generate O-methylated conjugates. The methyl metabolite C8H8O6S of PCA was detected in urine, while the methyl metabolites C8H8O6S, C8H8O7S, C6H6O5S, and C14H16O10 of PCA were detected in plasma. No metabolites were detected in the feces of rats [33], suggesting that this component is absorbed and metabolized in the bloodstream of rats and is not excreted through feces. An important basis for PCA in treating cognition is its relatively high permeability, enabling it to pass through the blood–brain barrier via passive diffusion [34]. Oral administration of a 50 mg/kg aqueous solution of PCA can reach the pharmacologically effective concentration of PCA in the body within 5 min and remains stable in human plasma for 24 h. However, it decomposes rapidly and in a dose-dependent manner in mouse plasma [35]. Additionally, PCA demonstrates favorable safety, inhibits apoptotic markers of liver cells, and reduces the extent of liver and kidney damage [36]. There are no studies reporting adverse effects on liver and kidney functions. The pharmacokinetics are depicted in Figure 3.

4. The Mechanisms by Which Protocatechuic Acid Treats Cognitive Impairment

Figure 4 displays the various mechanisms involved.

4.1. Regulation of the Expression of Neuroprotective Factors

It has been discovered in research that PCA can regulate the expression of neuroprotective factors to enhance cognition. Krzysztoforska, K. [37] experimentally demonstrated that in the brains of rats administered with PCA, the level of DA increased and the level of AGE decreased. PCA improves memory ability by reducing the level of AGE, decreasing Tet2, activating NF-κB and NLRP3, and enhancing intestinal permeability [38]. As an inhibitor of monoamine oxidase (MAO) and dopamine β-hydroxylase, PCA effectively elevates dopamine (DA) levels by obstructing the conversion of DA into norepinephrine (NA) via dopamine β-hydroxylase and preventing further oxidative deamination by MAO. This mechanism subsequently leads to the upregulation of downstream receptor gene expression for Drd1 and Drd4, mediated by PCA, thereby playing a crucial regulatory role in cognitive decline.
Krzysztoforska, K. [39] further discovered that PCA led to an elevation in histidine concentration within both the hippocampus and prefrontal cortex. This increase in histidine levels ameliorated mitochondrial damage induced by the synergistic effects of diabetic hypoglycemia, facilitating mitochondrial energy utilization for the comprehensive maintenance of calcium (Ca2+) homeostasis, neuronal growth, and axonal branching, thereby ensuring effective synaptic transmission [40]. This phenomenon is associated with the buffering and antioxidant properties of histidine; notably, histidine treatment significantly upregulates histamine receptors (H1R and H2R) following combined exposure to diabetic hypoglycemia—receptors that play crucial physiological roles in memory consolidation, sleep–wake regulation, appetite control, and stress response within the brain [41].
Song, Y. [42] demonstrated that PCA treatment enhances the expression of BDNF/pro-BDNF in the hippocampus and prefrontal cortex of ischemia-reperfusion rats. This intervention not only modulates the activity of BACE1 in these brain regions but also elevates ADAM10 activity, as well as increases phosphorylation levels of Akt at Ser473 in the hippocampus and GSK3β at Ser9 in both the hippocampus and prefrontal cortex. BDNF is known to regulate GSK3β signaling pathways, thereby contributing to cognitive function improvement [43].
Krzysztoforska, K. [44] reported that PCA administration significantly elevated 5-HT (5-HIAA/5-HT) turnover within the hypothalamus and prefrontal cortex. The activation of 5-HT1AR appears to reduce Iba1, GFAP, and TNF-α expression, indicating its role in inhibiting microglial and astrocytic activation induced by aβ1-42 [45].
Kale, S. [46] found that PCA administration markedly increased CREB expression in cortical tissues subjected to ischemia-reperfusion injury. Elevated CREB levels were associated with reduced ROS production and inhibited apoptosis among primary neurons in mice. Notably, CREB knockout downregulated components of the PGA-CAMKIV signaling pathway while decreasing PKA, CaMKIV, CREB, pCREB, arc, and c-fos protein expressions within primary neurons—ultimately impairing memory functions [47]. Thus, enhancing CREB expression can facilitate improvements in learning and memory capabilities.
In conclusion, PCA ameliorates cognitive dysfunction through elevations in DA, histidine, BDNF, 5-HT, and CREB levels while concurrently reducing AGE.

4.2. Inhibition of Cell Apoptosis

Yin, X. [48] discovered that PCA could increase the expression level of Bax and decrease the expression level of Bcl-2 by acting on the Bcl-2 family proteins in neuronal cells. The integrity of the mitochondrial membrane was altered, resulting in an increased release of ROS and cytochrome c, activating caspase-9 and caspase-3, thereby activating the mitochondrial apoptotic pathway. Additionally, the reduction in ROS induced by PCA could inhibit the activation of caspase-3, promote the proliferation of neural stem cells, and prevent the apoptosis of neural stem cells [49].
Kho, A.R. [50] employed the TSQ fluorescence method to reveal that PCA could lower the intracellular zinc level in the CA1 region of the hippocampus. It inhibits the mitochondrial energy processes, including the dissipation of the mitochondrial membrane potential (MMP), leading to ATP depletion and consequent neuronal death [51].
Liu, Y.M. [52] found that PCA could prevent the collapse of the mitochondrial membrane potential induced by ichthyotoxin and inhibit the opening of the mitochondrial permeability transition pore (PTP), thereby causing apoptotic substances such as cytochrome c to be released from the mitochondria into the cytoplasm, and subsequently inactivate caspase-3-like enzymes.
The mitogen-activated protein kinase (MAPK) superfamily comprises three members: the classical MAPK (also referred to as ERK), JNK, and p38 [53]. Yin, X. [54] discovered that PCA mainly treats cognitive disorders by inhibiting the JNK and P38 MAPK signal transduction pathways. PCA inhibits the apoptosis of hippocampal and prefrontal cortex neurons by reducing the phosphorylation of JNK/P38 and downstream targets c-fos, Bax, and cleaved caspase-3 in the hippocampus and prefrontal cortex. The pathway is depicted in Figure 5.

4.3. Inhibition of Oxidative Stress Injury

Due to the brain’s high oxygen utilization rate, high non-heme iron content that promotes the generation of reactive oxygen species (ROS), and high polyunsaturated fatty acids content, the brain is at high risk of oxidative damage [55]. PCA possesses dose-dependent antioxidant capacity. Compared with standard antioxidants, PCA exhibits more effective antioxidant potential in both lipid and aqueous media [56]. Protocatechuic acid is an effective phenolic acid antioxidant. Its antioxidant activity mainly stems from its phenolic hydroxyl group and the activation of endogenous antioxidant enzymes. It can inhibit lipid peroxidation and has moderate hydrogen peroxide and DPPH radical scavenging activity in vitro [57].
PCA treatment has been shown to attenuate the increase in xanthine oxidase (XOD) activity [54], thereby inhibiting XOD-catalyzed purine oxidation and the production of superoxide radicals as by-products.
In oxidative reactions, PCA is capable of inhibiting the overexpression of inducible nitric oxide synthase (iNOS) to generate a large amount of NO, thereby suppressing the reaction of NO with superoxide radicals (O2−) to form the peroxynitrite anion (ONOO−), inhibiting the oxidative reaction process and alleviating neuronal cell damage [58].
PCA elevates superoxide dismutase (SOD) [59] to catalyze the disproportionation of superoxide radicals (O2−) into hydrogen peroxide. Additionally, PCA can raise glutathione peroxidase (GSH-PX) [59] and catalase (CAT) [59], further converting hydrogen peroxide into water, thereby eliminating superoxide radicals within the body [60]. This decreases the oxidation of unsaturated fatty acids into lipid peroxides and subsequently inhibits the further decomposition of these peroxides into malondialdehyde (MDA), thereby reducing the reactions of malondialdehyde (MDA) with phospholipids and proteins to form neurotoxic lipofuscin [61], improving learning and memory.
PCA is able to lower hydrogen peroxide [62] levels and inhibit its reaction with intracellular reduced iron ions through the Fenton reaction to generate highly toxic hydroxyl radicals, suppressing toxicity to neuronal cells in brain tissue. Since neuronal membranes contain a large amount of oxidation substrates such as polyunsaturated fatty acids and have low catalase activity, they are more susceptible to oxidative damage than other parts [63].
Adeyanju, A.A. [64] discovered that PCA downregulates neuronal NF-κB expression. The activation of NF-κB functionally disrupts or inhibits p53 activity, thereby leading to a reduction in cisplatin-induced neurodegenerative damage. PCA exerts its antioxidant activity in the brain through NF-κB/P53 pathways. The pathway is shown in Figure 5.

4.4. Promotion of the Autophagy-Lysosome Pathway

Cellular autophagy refers to the process by which cells use lysosomes to degrade and recycle damaged organelles and macromolecular substances within the cell. Abnormalities in the autophagy-lysosome pathway, such as the deposition of Aβ1-42, the accumulation of p62 protein, and the hyperphosphorylation and aggregation of Tau, can all result in cognitive dysfunction. When the autophagy process of hippocampal neurons is abnormal, the homeostasis of neuronal cells is disrupted and the cognitive function of the body is affected. The presence of LAMP-1 enables autophagosomes to fuse with lysosomes, introducing the encapsulated cellular components into lysosomes for degradation [65].
P62 is a protein closely related to the autophagy process. As a linker protein, it helps combine the labeled intracellular waste with autophagosomes and promotes their degradation and clearance. When autophagy is activated, P62 is degraded in autolysosomes and can be continuously consumed as autophagy progresses [66], and its expression level indirectly reflects the level of autophagosome clearance.
The mammalian protein microtubule-associated protein 1 light chain 3 (LC3) can specifically bind to the autophagosome membrane and is located on the surface of pre-autophagosomes and autophagosomes, participating in the regeneration and extension of the autophagosome membrane. There are two convertible forms of LC3-I and LC3-II: When autophagy occurs, LC3-I processed by ubiquitination-like modification binds to phosphatidylethanolamine on the surface of the autophagosome membrane, thereby converting to LC3-II. They are involved in the formation of the autophagosome membrane, and this process marks the occurrence of autophagy [67].
Beclin 1 is a key protein in the autophagy process, which participates in the formation of autophagosomes and the regulation of autophagy [68].
Li, H. [69] demonstrated through immunofluorescence that PCA increased the fluorescence intensities of LAMP1 and lysosomes, enabling autophagosomes to fuse with lysosomes and introducing the encapsulated deposits of Aβ1-42, accumulated p62 protein, and hyperphosphorylated aggregates of Tau into lysosomes for degradation. WB showed an increase in the autophagy-related marker LC3-II/I, a significant decrease in the p62 level, and a significant increase in the levels of LAMP1 and CD proteins, indicating an increase in autophagy levels, a reduction in the deposition of Aβ1-42, the accumulation of p62 protein, and the hyperphosphorylated aggregates of Tau, thereby improving cognition.
Huang, L. [70] discovered that PCA significantly reduced the expression of Beclin-1, activated the akt/GSK-3β/MeF2d signaling pathway, activated Akt through inducing phosphorylation, and that the phosphorylation of Akt promoted the phosphorylation of GSK-3β, thereby reducing the inhibitory effect of GSK-3β on MEF2D. The increase in the nuclear concentration of MEF2D increased the expression of IL-10 and decreased the expressions of IL-1β, IL-6, and TNF-α. It induces protective autophagy to treat cognitive dysfunction. The pathway is shown in Figure 6.

4.5. Inhibition of Inflammation

Neuroinflammation refers to a series of immune responses mediated by neuroimmune cells within the central nervous system. Neuroimmune cells, including microglia and astrocytes, activate inflammatory mediators that can compromise the integrity of the blood–brain barrier and white matter in the brain, ultimately leading to cognitive impairment [71]. Amyloid-beta (Aβ) has been shown to activate pro-inflammatory pathways involving cyclophilin A and matrix metalloproteinase 9 (MMP-9) in pericytes, exacerbating inflammatory damage [72].
Xi, Z. [48] demonstrated that PCA downregulates both P38-ERK-JNK and NF-κB signaling pathways in treating cognitive impairment. PCA treatment inhibited phosphorylated MAPK production in a dose-dependent manner, prevented NF-κB translocation from the cytoplasm to the nucleus, and suppressed the expression levels of IL-1β, IL-6, and TNF-α.
Kaewmool, C. [73] found that PCA inhibited the release of IL-1β, IL-6, TNF-α, iNOS, and COX-2 in BV2 microglia through modulation of the SIRT1/NF-κB pathway; it increased SIRT1 levels while reducing p65 acetylation. This resulted in decreased neuroinflammation as well as protection for both the blood–brain barrier and white matter—ultimately improving cognitive function.

4.6. Improve Dysfunction of Synaptic Plasticity

Dendritic spines are the postsynaptic sites of excitatory synapses and an essential component of neuronal synaptic plasticity. As the physiological basis for the formation of cognitive functions, neural synaptic plasticity participates in the pathological process of cognitive impairment formation [74]. Lee, S.H. [75] discovered that the MAP2 immunoreactivity (IR) in the hippocampus and cortex of rats with traumatic brain injury treated with PCA significantly increased, suggesting that PCA inhibits the loss of dendrites. Guan, S. [76] experimentally demonstrated that the dendritic spine-like structures in the elongated neurites of differentiated cells in the PCA group were more prominent, indicating that PCA influences the terminal differentiation stage of neurons, induces neuronal maturation, and effectively promotes neurite growth.
Synaptophysin (SYP) is located on the synaptic vesicles of the presynaptic membrane. By binding to the lipid of the synaptic vesicle membrane, it promotes the movement of synaptic vesicles to the presynaptic membrane and the release of neurotransmitters— while also participating in the occurrence and differentiation of synapses—and is one of the indicators showing changes in synaptic plasticity [77]. Glial Fibrillary Acidic Protein (GFAP) is the support system or “scaffold” for cells and nuclei in astrocytes. One of its functions is to provide mechanical support to the plasma membrane that contacts other cells or the extracellular matrix. An increase in GFAP production can cause hypertrophy, proliferation, and migration of astrocytes, forming reactive astrogliosis [78]. Excessive gliosis promotes reactive glial cell proliferation-induced glial scarring, which acts as a mechanical barrier to hinder the regeneration of myelin and axons, affecting the structural repair and functional recovery of neural tissue [79]. Yin, X. [54] utilized immunohistochemistry and found that PCA elevated the SYP in neuronal cells of the hippocampus and prefrontal cortex of rats, promoting the movement of synaptic vesicles to the presynaptic membrane and the release of neurotransmitters, thereby facilitating the occurrence and differentiation of synapses; further, it reduced the expression of GFAP in the cortex and hippocampus, inhibited the formation of reactive astrogliosis, suppressed the generation of glial scars, promoted the regeneration of myelin and axons, and facilitated structural repair and functional recovery. Additionally, PCA can also promote the growth of dendritic spine-like structures in elongated neurites, reduce dendritic loss, be beneficial for neuronal synaptic plasticity, and thereby improve learning and memory.

4.7. Inhibition of Aβ Deposition and Reduction in APP Hydrolysis

Aβ is generated by proteolytic processing of amyloid precursor protein (APP) through β-secretase, β-site APP cleaving enzyme 1 (BACE1), and γ-secretase. Excessive extracellular Aβ levels in the brain can inhibit long-term potentiation (LTP) in the hippocampus and affect memory function [80]. Moreover, it leads to Aβ aggregation, forming soluble Aβ oligomers with high neurotoxicity that can further reaggregate into fibrils to form amyloid plaques [81]. The aggregation and accumulation of Aβ drive tau phosphorylation promote the formation of neurofibrillary tangles, and subsequently induce synaptic toxicity, neurotoxicity, and ultimately cognitive impairment [82]. Rummel, N.G. [83] discovered that small, highly hydrophobic Aβ oligomers cause lipid peroxidation, resulting in the production of 4-hydroxynonenal (HNE) that leads to oxidative modification and dysfunction of membrane lipids and cytosolic resident proteins. Oxidatively modified proteins can cause neuronal death as well as thinning of the hippocampus and frontal cortex, thereby resulting in cognitive impairment. Song, Y. [42] found that PCA could attenuate Aβ deposition and APP in the hippocampus and cerebral cortex of AβPP/PS1 mice. Huang, L. [70] demonstrated that the levels of p-tau, Aβ42, and β-secretase in the PCA treatment group decreased in a dose-dependent manner. Hornedo-Ortega, R. [84] observed using transmission electron microscopy that the number of Aβ1-42 aggregates decreased with increasing PCA concentration, and the monomeric form of α-synuclein increased.
In conclusion, PCA is capable of attenuating APP, reducing β-secretase, thereby reducing APP hydrolysis, lowering extracellular Aβ levels in the brain, inhibiting the formation of soluble Aβ oligomers with high neurotoxicity, suppressing tau phosphorylation, inhibiting the formation of neurofibrillary tangles and, subsequently, inhibiting synaptic and neurotoxicity, which is conducive to learning and memory.

4.8. Regulation of the Cholinergic System

Acetylcholine (ACh) is a crucial neurotransmitter in the central cholinergic system, capable of transmitting neuronal information and contributing to enhanced learning and memory abilities. The impairment of cholinergic neurons in the brain can result in cognitive disorders [85]. Under the action of choline acetyltransferase (ChAT), the acetyl group on acetyl coenzyme A is transferred to choline to generate acetylcholine. In the synaptic cleft, acetylcholine is degraded by acetylcholinesterase (AChE) and the activity changes in both enzymes jointly maintain the dynamic equilibrium of acetylcholine in brain tissue [86]. Acetylcholine can increase the activity of α-secretase, thereby facilitating the processing of APP along the non-amyloidogenic generation pathway and reducing the formation of Aβ [87].
Consequently, the PCA treatment group, by activating choline acetyltransferase [88] and significantly reducing the activity of acetylcholinesterase, elevates the level of acetylcholine in the synaptic cleft, improves normal neural transmission in rats [89], reduces the formation of Aβ and, subsequently, ameliorates cognitive impairments in vivo.

4.9. Inhibition of Excitotoxicity in Neuronal Cells

Glutamate exerts its physiological effects by binding and activating ligand-gated ion channels [ionotropic glutamate receptors (iGluRs)] and a class of G protein-coupled receptors [metabotropic glutamate receptors (mGluRs)]. Timely clearance of glutamate from the synaptic cleft is necessary. High levels of extracellular glutamate overactivate glutamate receptors, leading to excitotoxicity in the central nervous system. Additionally, the increase in extracellular glutamate concentration inhibits cystine uptake through the cystine/glutamate antiporter (system Xc-), resulting in glutathione depletion and glutamate oxidative toxicity [90]. NMDARs are ionotropic receptors with the highest affinity for glutamate and are double-gated channels with high calcium conductance. Excessive activation of NMDARs by glutamate leads to a large influx of Ca2+, activating various degradation processes—such as endonucleases involved in the necrotic process—and causing mitochondrial swelling and cytochrome c release from mitochondria, thereby initiating the apoptotic process that will lead to thinning of the hippocampus and frontal cortex brain regions and results in cognitive impairment [91]. Ban, J.Y. [92] found that PCA can inhibit the increase in Ca2+ concentration and glutamate induced by Aβ (25–35).
Therefore, PCA promotes astrocytes to recycle glutamate from the synaptic cleft through glutamate reuptake and then converts it to glutamine via glutamine synthetase, reducing the level of glutamate in the synaptic cleft and inhibiting glutamate receptors. On the one hand, it reduces the excitotoxicity of neuronal cells, and on the other hand, it inhibits the influx of Ca2+, thereby inhibiting various degradation processes, preventing mitochondrial swelling, inhibiting the release of cytochrome c from mitochondria—thus preventing the initiation of the apoptotic process, protecting the hippocampus and frontal cortex, and improving cognitive ability.
The glutamate and GABAergic systems are representative excitatory and inhibitory nervous systems, and their interaction and maintenance of a balanced state have significant implications for maintaining learning and memory functions. The weakened inhibitory function of GABAergic neurons can cause excessive excitation of excitatory neurons, thereby disrupting the excitation–inhibition balance state, generating excitotoxicity, ultimately triggering abnormal activities at the neural network level and leading to cognitive dysfunction [93]. Mert, H. [94] experimentally demonstrated that PCA can increase the GABA level in the hippocampus.
Therefore, PCA can increase the GABA level in the hippocampus, enhance the inhibitory excitatory function, and weaken excitotoxicity. Adefegha [95] found that PCA can significantly increase the activity of Na+/K+ ATPase, thereby improving the dysfunction of the Na-K pump under conditions of brain tissue ischemia and hypoxia, inhibiting the disorder of Na+ and K+ concentrations inside and outside cells, inducing the generation of various enzymes and ATP, preventing the activation of voltage-sensitive Ca2+ channels, preventing a large influx of Ca2+, reducing the release of neurotransmitters such as glutamate, and reducing the cytotoxic effects on neuronal cells, which is beneficial for cognitive dysfunction [96].
In summary, PCA can significantly increase the activity of Na+/K+ ATPase, thereby improving the dysfunction of the Na-K pump under conditions of brain tissue ischemia and hypoxia, inhibiting the disorder of Na+ and K+ concentrations inside and outside cells, inducing the generation of various enzymes and ATP, preventing the activation of voltage-sensitive Ca2+ channels, preventing a large influx of Ca2+, reducing the release of neurotransmitters such as glutamate, and reducing the cytotoxic effects on neuronal cells. At the same time, PCA promotes astrocytes to recycle glutamate from the synaptic cleft through glutamate reuptake and then converts it to glutamine via glutamine synthetase, reducing the level of glutamate in the synaptic cleft and inhibiting glutamate receptors, reducing the excitotoxicity of neuronal cells. PCA can also increase the GABA level in the hippocampus, enhance the inhibitory excitatory function, and weaken excitotoxicity. Through the above three mechanisms, PCA weakens the cytotoxic effects on neuronal cells and improves cognitive dysfunction.

4.10. Inhibition of Ferroptosis

Ferroptosis is an iron-dependent novel form of programmed cell death, distinct from apoptosis and autophagy, and is associated with various diseases such as degenerative disorders and stroke. The main mechanism of ferroptosis is that, under the action of divalent iron or lipoxygenase, the highly expressed unsaturated fatty acids on the cell membrane are catalyzed, resulting in lipid peroxidation and thereby inducing cell death. Additionally, it is characterized by glutathione depletion, a reduction in the core regulatory enzyme GPX4 of the antioxidant system (glutathione system), and accompanied by elevated levels of reactive oxygen species and malondialdehyde. Muley MM et al. discovered that PCA induces the phosphorylation of the transcription factor Nrf2 mediated by JNK. Along with the intense activation of the transcription factor, a large amount of Nrf2 translocates to the nucleus, increasing the level of active phosphorylated JNK and enhancing glutathione reductase (GR) [97], glutathione peroxidase (GPX) [59], and the ferroptosis factor glutathione (GSH) [50] in neural stem cells, while reducing lipid hydroperoxide (LPO) [62] and the ferroptosis factor malonic dialdehyde (MDA) [98] in neural stem cells. GPX4, as an antioxidant defense enzyme, is regarded as a key regulatory factor for inhibiting ferroptosis and can eliminate excessive toxic lipid hydroperoxides, thereby improving oxidative stress damage in cells; however, this process requires the participation of glutathione as a cofactor [99]. Glutathione, as the substrate of GPX4, participates in the intracellular antioxidant system. Direct or indirect inhibition of glutathione biosynthesis can decrease GPX4 activity and promote the occurrence of ferroptosis [100]. Moreover, GSH is also a natural ligand for Fe2+ in the labile iron pool (LIP) of neurons. The binding of GSH to Fe2+ in LIP prevents iron oxidation, which not only maintains the solubility of Fe2+ but also prevents Fe2+ from generating hydroxyl radicals as a catalyst. The inhibition of GSH synthesis directly or indirectly reduces the antioxidant capacity of cells [101]. Nrf2 is a key target for alleviating cell damage and also a crucial regulatory factor for ferroptosis [102]. Studies have shown that nuclear factor erythroid 2-related factor 2 (Nrf2) can promote the expression of GSH, GR, and GPX4, increasing the antioxidant capacity of cells. On the other hand, Nrf2 can also inhibit the occurrence of ferroptosis by promoting the transport of intracellular iron ions and reducing the accumulation of intracellular iron ions [103].
Therefore, the mechanism by which PCA inhibits ferroptosis is as follows: PCA induces the phosphorylation of the transcription factor Nrf2 mediated by JNK. Along with the intense activation of the transcription factor, a large amount of Nrf2 translocates to the nucleus, increasing the level of active phosphorylated JNK and promoting the biosynthesis of GSH. On the one hand, it increases the binding of GSH to Fe2+ in LIP to prevent iron oxidation, inhibits the catalysis of highly expressed unsaturated fatty acids on the cell membrane under the action of divalent iron or lipoxygenase, and suppresses lipid peroxidation, thereby protecting neuronal cells; on the other hand, it promotes the synthesis of GPX4, converting the peroxide bonds of lipid peroxidation into hydroxyl groups, eliminating excessive toxic lipid hydroperoxides, and maintaining the homeostasis of the membrane lipid bilayer, thereby protecting neuronal cells and improving cognitive dysfunction.

5. Summary

With the growth of the elderly population and the extension of life expectancy, the issue of age-related neurodegenerative processes will spread and exert social and economic influences. Hence, seeking new therapeutic approaches has become an indispensable task for modern medicine.
This article, by summarizing the mechanism of action of PCA in cognitive impairment, discovers that PCA can function through multiple pathways such as regulating the expression of neuroprotective factors, inhibiting apoptosis, promoting the autophagy-lysosome pathway, suppressing oxidative stress damage, inhibiting inflammatory responses, ameliorating synaptic plasticity dysfunction, inhibiting Aβ deposition and reducing APP hydrolysis, improving the cholinergic system, suppressing excitotoxicity of neuronal cells, and inhibiting ferroptosis. The action pathways involve activating Pi3K-akt-mTor and inhibiting JNK, P38 MAPK, P38-ERK-JNK, SIRT1, and NF-κB/p53, etc. The action pathways of PCA are extensive, encompassing multiple pathways and targets. However, as the current research on PCA is in its infancy, there are relatively few clinical and basic studies on the application of PCA in the treatment of patients with cognitive impairment. Research on the safety, toxicity, teratogenicity, and carcinogenicity of PCA is lacking. Moreover, the research mechanisms for inhibiting the effects of certain pathways are still needed, and further experiments and clinical studies are required to fully determine the therapeutic efficacy of PCA.

Author Contributions

Conceptualization, S.L. and Z.Z.; methodology, L.L.; validation, S.L.; formal analysis, Z.Z.; investigation, L.L.; data curation, S.L.; writing—original draft preparation, L.L.; writing—review and editing, S.L.; visualization, Z.Z.; supervision, S.L.; project administration, Z.Z.; funding acquisition, Y.Z. and X.L. All authors have read and agreed to the published version of the manuscript.

Funding

This research was financially supported by the National Natural Science Foudation of China (82374330).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are included in the article.

Conflicts of Interest

The authors declare no conflicts of interest.

Abbreviation List

AbbreviationFull titleAbbreviationFull title
CICognitive impairmentPCAProtocatechuic acid
AGEsAdvanced glycation end productsDADopamine
5-HT5-hydroxytryptamineBDNFBrain-derived neurotrophic factor
CREBcAMP-response element binding proteinNF-κBNuclear factor κB
PKAProtein kinase A systemXODXanthine oxidase
LC3Microtubule-associated protein 1 light chain 3MMP-9Matrix metalloproteinase 9
HNE4-hydroxynonenalChATCholine acetyltransferase
AChEAcetylcholinesteraseGRGlutathione reductase
GPXGlutathione peroxidaseGSHGlutathione
LPOLipid hydroperoxideMDAMalonic dialdehyde
LIPLabile iron pool

References

  1. Moritz, S.; Silverstein, S.M.; Dietrichkeit, M.; Gallinat, J. Neurocognitive deficits in schizophrenia are likely to be less severe and less related to the disorder than previously thought. World Psychiatry 2020, 19, 254–255. [Google Scholar] [CrossRef] [PubMed]
  2. Berron, D.; Vogel, J.W.; Insel, P.S. Early stages of tau pathology and its associations with functional connectivity, atrophy and memory. Brain 2021, 144, 2771–2783. [Google Scholar] [CrossRef] [PubMed]
  3. Skovgård, K.; Agerskov, C.; Kohlmeier, K.A.; Herrik, K.F. The 5-HT3 receptor antagonist ondansetron potentiates the effects of the acetylcholinesterase inhibitor donepezil on neuronal network oscillations in the rat dorsal hippocampus. Neuropharmacology 2018, 143, 130–142. [Google Scholar] [CrossRef]
  4. Bernal-Mercado, A.T.; Vazquez-Armenta, F.J.; Tapia-Rodriguez, M.R. Comparison of Single and Combined Use of Catechin, Protocatechuic, and Vanillic Acids as Antioxidant and Antibacterial Agents against Uropathogenic Escherichia Coli at Planktonic and Biofilm Levels. Molecules 2018, 23, 2813. [Google Scholar] [CrossRef] [PubMed]
  5. Lin, H.; Chen, J.; Chou, F.; Wang, C. Protocatechuic acid inhibits cancer cell metastasis involving the down-regulation of Ras/Akt/NF-κB pathway and MMP-2 production by targeting RhoB activation. Br. J. Pharmacol. 2011, 162, 237–254. [Google Scholar] [CrossRef] [PubMed]
  6. Lin, C.; Huang, C.; Huang, C.; Yin, M. Anticoagulatory, antiinflammatory, and antioxidative effects of protocatechuic acid in diabetic mice. J. Agric. Food Chem. 2009, 57, 6661–6667. [Google Scholar] [CrossRef]
  7. Szwajgier, D.; Borowiec, K.; Pustelniak, K. The Neuroprotective Effects of Phenolic Acids: Molecular Mechanism of Action. Nutrients 2017, 9, 477. [Google Scholar] [CrossRef]
  8. Trinha, L.T.P.; Choic, Y.S.; Baea, H.J. Production of phenolic compounds and biosugars from flower resources via several extraction processes. Ind. Crop. Prod. 2018, 125, 261–268. [Google Scholar] [CrossRef]
  9. Gruz, J.; Ayaz, F.A.; Torun, H.; Strnad, M. Phenolic acid content and radical scavenging activity of extracts from medlar (Mespilus germanica L.) fruit at different stages of ripening. Food Chem. 2011, 124, 271–277. [Google Scholar] [CrossRef]
  10. Szopa, A.; Kokotkiewicz, A.; Bednarz, M.; Luczkiewicz, M.; Ekiert, H. Studies on the accumulation of phenolic acids and flavonoids in different in vitro culture systems of Schisandra chinensis (Turcz.) Baill.using a DAD-HPLC method. Phytochem. Lett. 2017, 20, 462–469. [Google Scholar] [CrossRef]
  11. Wei, L.; Zhu, P.; Chen, X.; Wang, Y.; Xu, Y. An ultra high performance liquid chromatography with tandem mass spectrometry method for simultaneous determination of thirteen components extracted from Radix Puerariae in rat plasma and tissues: Application to pharmacokinetic and tissue distribution study. J. Sep. Sci. 2020, 43, 418–437. [Google Scholar] [PubMed]
  12. Yang, Y.; Wei, M.; Huang, T.; Lee, S. Extraction of protocatechuic acid from Scutellaria barbata D. Don using supercritical carbon dioxide. J. Supercrit. Fluids 2013, 81, 55–66. [Google Scholar] [CrossRef]
  13. Hou, P.; Wang, Q.; Qi, W.; Zhang, Y.; Xie, J. Comprehensive determination of seven polyphenols in Eucommia ulmoides and its anti oxidative stress activity in C. elegans. J. Food Meas. Charact. 2019, 13, 2903–2909. [Google Scholar] [CrossRef]
  14. Li, Q.; Jia, Y.; Xu, L. Simultaneous determination of protocatechuic acid, syringin, chlorogenic acid, caffeic acid, liriodendrin and isofraxidin in Acanthopanax senticosus Harms by HPLC-DAD. Biol. Pharm. Bull. 2006, 29, 532–534. [Google Scholar] [CrossRef] [PubMed]
  15. Yang, C.; Tang, Q.; Liu, J. Preparative isolation and purification of phenolic acids from Smilax china by high-speed counter-current chromatography. Sep. Purif. Technol. 2008, 61, 477–478. [Google Scholar] [CrossRef]
  16. Mîrza, C.M.; Mîrza, T.V.; Odagiu, A.C.M. Phytochemical Analysis and Antioxidant Effects of Prunella vulgaris in Experimental Acute Inflammation. Int. J. Mol. Sci. 2024, 25, 4843. [Google Scholar] [CrossRef]
  17. Lion, Q.; Pichette, A.; Mihoub, M.; Mshvildadze, V.; Legault, J. Phenolic Extract from Aralia nudicaulis L. Rhizomes Inhibits Cellular Oxidative Stresses. Molecules 2021, 26, 4458. [Google Scholar] [CrossRef]
  18. Chen, X.; Zhu, P.; Liu, B.; Wei, L.; Xu, Y. Simultaneous determination of fourteen compounds of Hedyotis diffusa Willd extract in rats by UHPLC-MS/MS method: Application to pharmacokinetics and tissue distribution study. J. Pharm. Biomed. Anal. 2018, 159, 490–512. [Google Scholar] [CrossRef]
  19. Xu, Y.; Wang, Y.; Bi, S. Simultaneous Determination of Four Ingredients in Plantago Depressa by Single Marker. J. Chem. 2021, 2021, 4040239. [Google Scholar] [CrossRef]
  20. Shi, J.; Peng, L.; Chen, W. Evaluation of chemical components and quality in Xinhui Chenpi (Citrus reticulata ‘Chachi’) with two different storage times by GC–MS and UPLC. Food Sci. Nutr. 2024, 12, 5036–5051. [Google Scholar] [CrossRef]
  21. Yener, İ.; Ertaş, A.; Yilmaz, M.A. Characterization of the Chemical Profile of Euphorbia Species from Turkey by Gas Chromatography–Mass Spectrometry (GC-MS), Liquid Chromatography–Tandem Mass Spectrometry (LC-MS/MS), and Liquid Chromatography–Ion Trap–Time-of-Flight–Mass Spectrometry (LC-IT-TOF-MS) and Chemometric Analysis. Anal. Lett. 2019, 52, 1031–1049. [Google Scholar]
  22. Jin, H.; Tang, G.; Li, J.; Ma, L.; Li, Y.; Chang, Y. Simultaneous Determination of Phenolic Acids, Anthraquinones, Flavonoids, and Triterpenes of Cynomorii Herba in Different Harvest Times by LC-MS/MS. J. Anal. Methods Chem. 2020, 2020, 8861765. [Google Scholar] [CrossRef] [PubMed]
  23. Jargalsaikhan, G.; Wu, J.; Chen, Y.; Yang, L.; Wu, M. Comparison of the Phytochemical Properties, Antioxidant Activity and Cytotoxic Effect on HepG2 Cells in Mongolian and Taiwanese Rhubarb Species. Molecules 2021, 26, 1217. [Google Scholar] [CrossRef] [PubMed]
  24. Pan, Y.; Zhang, L.; Chen, G. Separation and determination of protocatechuic aldehyde and protocatechuic acid in Salivia miltorrhrza by capillary electrophoresis with amperometric detection. Analyst 2001, 126, 1519–1523. [Google Scholar] [CrossRef]
  25. Zhou, Z.; Liang, S.; Zou, X.; Teng, Y.; Wang, W.; Fu, L. Determination of Phenolic Acids Using Ultra-High-Performance Liquid Chromatography Coupled with Triple Quadrupole (UHPLC-QqQ) in Fruiting Bodies of Sanghuangporus baumii (Pilát) L.W. Zhou and Y.C. Dai. Plants 2023, 12, 3565. [Google Scholar] [CrossRef] [PubMed]
  26. Osmic, N.; Culum, D.; Ibragic, S. Catechins and other phenolic compounds in herb of eight Ephedra species in comparison to Camellia sinensis. Nat. Prod. Res. 2024, 38, 1457–1462. [Google Scholar] [CrossRef] [PubMed]
  27. Zhang, J.; Li, Y.; Lin, S.; Li, H. Green Extraction of Natural Antioxidants from the Sterculia nobilis Fruit Waste and Analysis of Phenolic Profile. Molecules 2018, 23, 1059. [Google Scholar] [CrossRef]
  28. Muhammad, D.R.A.; Tuenter, E.; Patria, G.D.; Foubert, K.; Pieters, L.; Dewettinck, K. Phytochemical composition and antioxidant activity of Cinnamomum burmannii Blume extracts and their potential application in white chocolate. Food Chem. 2021, 340, 127983. [Google Scholar] [CrossRef]
  29. Tian, L.; Tan, Y.; Chen, G. Metabolism of anthocyanins and consequent effects on the gut microbiota. Crit. Rev. Food Sci. Nutr. 2019, 59, 982–991. [Google Scholar] [CrossRef]
  30. Tang, Y.; Nakashima, S.; Saiki, S. 3,4-Dihydroxyphenylacetic acid is a predominant biologically-active catabolite of quercetin glycosides. Food Res. Int. 2016, 89, 716–723. [Google Scholar] [CrossRef]
  31. Wang, X.; Yan, K.; Ma, X. Simultaneous Determination and Pharmacokinetic Study of Protocatechuic Aldehyde and Its Major Active Metabolite Protocatechuic Acid in Rat Plasma by Liquid Chromatography-Tandem Mass Spectrometry. J. Chromatogr. Sci. 2016, 54, 697–705. [Google Scholar] [CrossRef] [PubMed]
  32. Valentová, K.; Vrba, J.; Bancířová, M.; Ulrichová, J.; Křen, V. Isoquercitrin: Pharmacology, toxicology, and metabolism. Food Chem. Toxicol. 2014, 68, 267–282. [Google Scholar] [CrossRef] [PubMed]
  33. Ma, F.; Deng, Q.; Zhou, X. The Tissue Distribution and Urinary Excretion Study of Gallic Acid and Protocatechuic Acid after Oral Administration of Polygonum Capitatum Extract in Rats. Molecules 2016, 21, 399. [Google Scholar] [CrossRef] [PubMed]
  34. Xiao, T.; Pan, M.; Wang, Y. In vitro bloodbrain barrier permeability study of four main active ingredients from Alpiniae oxyphyllae fructus. J. Pharm. Biomed. Anal. 2023, 235, 115637. [Google Scholar] [CrossRef] [PubMed]
  35. Chen, W.; Wang, D.; Wang, L. Pharmacokinetics of protocatechuic acid in mouse and its quantification in human plasma using LC-tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 2012, 908, 39–44. [Google Scholar] [CrossRef]
  36. Habib, S.A.; Suddek, G.M.; Abdel, R.M.; Abdelrahman, R.S. The protective effect of protocatechuic acid on hepatotoxicity induced by cisplatin in mice. Life Sci. 2021, 277, 119485. [Google Scholar] [CrossRef]
  37. Krzysztoforska, K.; Piechal, A.; Blecharz-Klin, K. Administration of protocatechuic acid affects memory and restores hippocampal and cortical serotonin turnover in rat model of oral D-galactose-induced memory impairment. Behav. Brain Res. 2019, 368, 111896. [Google Scholar] [CrossRef]
  38. Luo, L.; Li, R.; Wang, G. Age-dependent effects of a high-fat diet combined with dietary advanced glycation end products on cognitive function and protection with voluntary exercise. Food Funct. 2022, 13, 4445–4458. [Google Scholar] [CrossRef]
  39. Krzysztoforska, K.; Piechal, A.; Blecharz-Klin, K. Effect of protocatechuic acid on cognitive processes and central nervous system neuromodulators in the hippocampus, prefrontal cortex, and striatum of healthy rats. Nutr. Neurosci. 2022, 25, 1362–1373. [Google Scholar] [CrossRef]
  40. Li, S.; Sheng, Z. Energy matters: Presynaptic metabolism and the maintenance of synaptic transmission. Nat. Rev. Neurosci. 2022, 23, 4–22. [Google Scholar] [CrossRef]
  41. Jurič, D.M.; Kržan, M.; Lipnik-Stangelj, M. Histamine and astrocyte function. Pharmacol. Res. 2016, 111, 774–783. [Google Scholar] [CrossRef] [PubMed]
  42. Song, Y.; Cui, T.; Xie, N.; Zhang, X.; Qian, Z.; Liu, J. Protocatechuic acid improves cognitive deficits and attenuates amyloid deposits, inflammatory response in aged AβPP/PS1 double transgenic mice. Int. Immunopharmacol. 2014, 20, 276–281. [Google Scholar] [CrossRef] [PubMed]
  43. Baranowski, B.J.; Mohammad, A.; Finch, M.S. Exercise training and BDNF injections alter amyloid precursor protein (APP) processing enzymes and improve cognition. J. Appl. Physiol. 2023, 135, 121–135. [Google Scholar] [CrossRef] [PubMed]
  44. Krzysztoforska, K.; Piechal, A.; Wojnar, E. Protocatechuic Acid Prevents Some of the Memory-Related Behavioural and Neurotransmitter Changes in a Pyrithiamine-Induced Thiamine Deficiency Model of Wernicke-Korsakoff Syndrome in Rats. Nutrients 2023, 15, 625. [Google Scholar] [CrossRef]
  45. Wang, M.; Zong, H.; Chang, K. 5-HT1AR alleviates Aβ-induced cognitive decline and neuroinflammation through crosstalk with NF-κB pathway in mice. Int. Immunopharmacol. 2020, 2020, 106354. [Google Scholar] [CrossRef]
  46. Kale, S.; Sarode, L.P.; Kharat, A. Protocatechuic Acid Prevents Early Hour Ischemic Reperfusion Brain Damage by Restoring Imbalance of Neuronal Cell Death and Survival Proteins. J. Stroke Cerebrovasc. Dis. 2021, 30, 105507. [Google Scholar] [CrossRef]
  47. Xing, J.; Han, D.; Xu, D.; Li, X.; Sun, L. CREB Protects against Temporal Lobe Epilepsy Associated with Cognitive Impairment by Controlling Oxidative Neuronal Damage. Neurodegener. Dis. 2019, 19, 225–237. [Google Scholar] [CrossRef]
  48. Xi, Z.; Hu, X.; Chen, X. Protocatechuic acid exerts protective effects via suppression of the P38/JNK- NF-κB signalling pathway in an experimental mouse model of intracerebral haemorrhage. Eur. J. Pharmacol. 2019, 854, 128–138. [Google Scholar] [CrossRef]
  49. Guan, S.; Ge, D.; Liu, T.; Ma, X.; Cui, Z. Protocatechuic acid promotes cell proliferation and reduces basal apoptosis in cultured neural stem cells. Toxicol. Vitr. 2009, 23, 201–208. [Google Scholar] [CrossRef]
  50. Kho, A.R.; Choi, B.Y.; Lee, S. Effects of Protocatechuic Acid (PCA) on Global Cerebral Ischemia-Induced Hippocampal Neuronal Death. Int. J. Mol. Sci. 2018, 19, 1420. [Google Scholar] [CrossRef]
  51. Liu, H.; Gale, J.; Reynolds, I.; Weiss, J.; Aizenman, E. The Multifaceted Roles of Zinc in Neuronal Mitochondrial Dysfunction. Biomedicines 2021, 9, 489. [Google Scholar] [CrossRef] [PubMed]
  52. Liu, Y.; Jiang, B.; Bao, Y.; An, L. Protocatechuic acid inhibits apoptosis by mitochondrial dysfunction in rotenone-induced PC12 cells. Toxicol. Vitr. 2008, 22, 430–437. [Google Scholar] [CrossRef] [PubMed]
  53. Ou, X.; Li, S.; Xu, B. p38α MAPK is a MTOC-associated protein regulating spindle assembly, spindle length and accurate chromosome segregation during mouse oocyte meiotic maturation. Cell Cycle 2010, 9, 4130–4143. [Google Scholar] [CrossRef] [PubMed]
  54. Yin, X.; Zhang, X.; Lv, C. Protocatechuic acid ameliorates neurocognitive functions impairment induced by chronic intermittent hypoxia. Sci. Rep. 2015, 5, 14507. [Google Scholar] [CrossRef]
  55. Halliwell, B. Oxidative stress and neurodegeneration: Where are we now? J. Neurochem. 2006, 97, 1634–1658. [Google Scholar] [CrossRef]
  56. Kakkar, S.; Bais, S. A review on protocatechuic Acid and its pharmacological potential. ISRN Pharmacol. 2014, 2014, 952943. [Google Scholar] [CrossRef]
  57. Han, l.; Yang, Q.; Ma, W. Protocatechuic acid ame-liorated palmitic-acid-induced oxidative damage in en-dothelial cells through activating endogenous antioxi-dant enzymes via an adenosine-monophosphat e-activated-protein-kinase-dependent pathway. J. Agric. Food chem. 2018, 66, 10400–10409. [Google Scholar] [CrossRef]
  58. Choi, J.R.; Kim, J.H.; Lee, S.; Cho, E.J.; Kim, H.Y. Protective effects of protocatechuic acid against cognitive impairment in an amyloid beta-induced Alzheimer’s disease mouse model. Food Chem. Toxicol. 2020, 144, 111571. [Google Scholar] [CrossRef]
  59. Li, Z.; Liu, Y.; Wang, F. Neuroprotective effects of protocatechuic acid on sodium arsenate induced toxicity in mice: Role of oxidative stress, inflammation, and apoptosis. Chem. Biol. Interact. 2021, 337, 109392. [Google Scholar] [CrossRef]
  60. Tobón-Velasco, J.C.; Carmona-Aparicio, L.; Ali, S.F. Biomarkersof cell damage induced by oxidative stress in Parkinson’s disease andrelated models. Cent. Nerv. Syst. Agents. Med. Chem. 2010, 10, 278–286. [Google Scholar] [CrossRef]
  61. Husain, K.; Mejia, J.; Lalla, J.; Kazin, S. Dose response of alcohol-in-duced changes in BP, nitric oxide and antioxidants in rat plasma. Pharmacol. Res. 2005, 51, 337–343. [Google Scholar] [CrossRef]
  62. Adedara, I.A.; Fasina, O.B.; Ayeni, M.F.; Ajayi, O.M.; Farombi, E.O. Protocatechuic acid ameliorates neurobehavioral deficits via suppression of oxidative damage, inflammation, caspase-3 and acetylcholinesterase activities in diabetic rats. Food Chem. Toxicol. 2019, 125, 170–181. [Google Scholar] [CrossRef] [PubMed]
  63. Lin, M.T.; Beal, M.F. Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature 2006, 443, 787–795. [Google Scholar] [CrossRef] [PubMed]
  64. Adeyanju, A.A.; Oso, B.J.; Molehin, O.R. Treatment with protocatechuic acid attenuates cisplatin-induced toxic-ity in the brain and liver of male wistar rats. Adv. Tradit. Med. 2023, 23, 121–131. [Google Scholar] [CrossRef]
  65. Eskelinen, E.L. Roles of LAMP-1 and LAMP-2 in lysosome biogenesis and autophagy. Mol. Asp. Med. 2006, 27, 495–502. [Google Scholar] [CrossRef] [PubMed]
  66. Huang, Y.; Tao, W.; Wang, J. Progress of researches on acupuncture treatment of cerebral ischemia-reperfusion injury by regulating autophagy in the ischemic cerebral tissue. Zhen Ci Yan Jiu 2019, 44, 459–464. [Google Scholar]
  67. Old, E.A.; Clark, A.K.; Malcangio, M. The role of glia in the spinal cord in neuropathic and inflammatory pain. Handb. Exp. Pharmacol. 2015, 227, 145–170. [Google Scholar]
  68. Cheng, X.; Xie, Y.; Zhou, B.; Huang, N.; Farfel-Becker, T.; Sheng, Z. Characterization of LAMP1-labeled nondegradative lysosomal and endocytic compartments in neurons. J. Cell Biol. 2018, 217, 3127–3139. [Google Scholar] [CrossRef]
  69. Li, H.; Zheng, T.; Lian, F.; Xu, T.; Yin, W.; Jiang, Y. Anthocyanin-rich blueberry extracts and anthocyanin metabolite protocatechuic acid promote autophagy-lysosomal pathway and alleviate neurons damage in in vivo and in vitro models of Alzheimer’s disease. Nutrition 2022, 93, 111473. [Google Scholar] [CrossRef]
  70. Huang, L.; Zhong, X.; Qin, S.; Deng, M. Protocatechuic acid attenuates β secretase activity and okadaic acid induced autophagy via the Akt/GSK 3β/MEF2D pathway in PC12 cells. Mol. Med. Rep. 2020, 21, 1328–1335. [Google Scholar] [CrossRef]
  71. Xu, X.; Zheng, X.; Zhou, Q.; Li, H. Inhibition of excitatory amino acid efflux contributes to protective effects of puerarin against cerebral ischemia in rats. Biomed. Environ. Sci. 2007, 20, 336–342. [Google Scholar] [PubMed]
  72. Bell, R.D.; Winkler, E.A.; Singh, I. Apolipoprotein E controls cerebrovascular integrity via cyclophilin A. Nature 2012, 485, 512–516. [Google Scholar] [CrossRef] [PubMed]
  73. Kaewmool, C.; Kongtawelert, P.; Phitak, T.; Pothacharoen, P.; Udomruk, S. Protocatechuic acid inhibits inflammatory responses in LPS-activated BV2 microglia via regulating SIRT1/NF-κB pathway contributed to the suppression of microglial activation-induced PC12 cell apoptosis. J. Neuroimmunol. 2020, 341, 577164. [Google Scholar] [CrossRef] [PubMed]
  74. Bourne, J.N.; Harris, K.M. Balancing structure and function at hippocampal dendritic spines. Annu. Rev. Neurosci. 2008, 31, 47–67. [Google Scholar] [CrossRef]
  75. Lee, S.; Choi, B.; Lee, S. Administration of Protocatechuic Acid Reduces Traumatic Brain Injury-Induced Neuronal Death. Int. J. Mol. Sci. 2017, 18, 2510. [Google Scholar] [CrossRef]
  76. Guan, S.; Zhang, X.; Ge, D.; Liu, T.; Ma, X.; Cui, Z. Protocatechuic acid promotes the neuronal differentiation and facilitates survival of phenotypes differentiated from cultured neural stem and progenitor cells. Eur. J. Pharmacol. 2011, 670, 471–478. [Google Scholar] [CrossRef]
  77. Murphy, T.H.; Corbett, D. Plasticity during stroke recovery: From synapse to behaviour. Nat. Rev. Neurosci. 2009, 10, 861–872. [Google Scholar] [CrossRef]
  78. Duan, S.; Sawyer, T.W.; Sontz, R.A.; Wieland, B.A.; Diaz, A.F.; Merchant, J.L. GFAP-directed Inactivation of Men1 Exploits Glial Cell Plasticity in Favor of Neuroendocrine Reprogramming. Cell. Mol. Gastroenterol. Hepatol. 2022, 14, 1025–1051. [Google Scholar] [CrossRef]
  79. Albrecht, P.J.; Dahi, J.P.; Stoltfus, O.K. Ciliary neurophicfactor activates spinal cord astrocytes stimulating their produc-tion and release of firoblast growth factor-2 to increase motorneutron surbvival. J. Exp. Nerrol. 2002, 173, 46–62. [Google Scholar] [CrossRef]
  80. Li, S.; Jin, M.; Koeglsperger, T. Soluble abeta oligomers inhibit long-term potentiation through a mechanism involving excessive activation of extrasynaptic NR2B-containing NMDA receptors. J. Neurosci. 2011, 31, 6627–6638. [Google Scholar] [CrossRef]
  81. Tolar, M.; Hey, J.; Power, A.; Abushakra, S. Neurotoxic Soluble Amyloid Oligomers Drive Alzheimer’s Pathogenesis and Represent a Clinically Validated Target for Slowing Disease Progression. Int. J. Mol. Sci. 2021, 22, 6355. [Google Scholar] [CrossRef]
  82. Karran, E.; Mercken, M. The amyloid cascade hypothesis for Alzheimer’s disease: An appraisal for the development of therapeutics. Nat. Rev. Drug Discov. 2011, 10, 698–712. [Google Scholar] [CrossRef]
  83. Rummel, N.G.; Butterfield, D.A. Altered metabolism in Alzheimer disease brain: Role of oxidative stress. Antioxid. Redox Signal. 2022, 36, 1289–1305. [Google Scholar] [CrossRef] [PubMed]
  84. Hornedo-Ortega, R.; Álvarez-Fernández, M.A.; Cerezo, A.B.; Richard, T.; Troncoso, A.M.A.; Garcia-Parrilla, M.A.C. Protocatechuic Acid: Inhibition of Fibril Formation, Destabilization of Preformed Fibrils of Amyloid-β and α-Synuclein, and Neuroprotection. J. Agric. Food Chem. 2016, 64, 7722–7732. [Google Scholar] [CrossRef] [PubMed]
  85. Xie, Y.; Liu, L.; Zheng, J. Polygoni Multiflori Radix Praeparata and Acori Tatarinowii Rhizoma ameliorate scopolamine-induced cognitive impairment by regulating the cholinergic and synaptic associated proteins. J. Ethnopharmacol. 2023, 311, 116400. [Google Scholar] [CrossRef]
  86. Nan, Z.; Dan, L.; Yi, W. Screening and identification of anti-acetylcholinesterase ingredients from tianzhi granule based on ultrafiltration combined with ultra-performance liquid chromatography-mass spectrometry and in silico analysis. J. Ethnopharmacol. 2022, 298, 115641. [Google Scholar]
  87. Yamini, P.; Ray, R.S.; Yadav, S. α7nAChR activation protects against oxidative stress, neuroinflammation and central insulin resistance in ICV-STZ induced sporadic Alzheimer’s disease. Pharmacol. Biochem. Behav. 2022, 217, 173402. [Google Scholar] [CrossRef]
  88. Kang, T.; Yang, Q.; Bais, S. Neuroprotective effect of procate-chuic acid through MaO-B inhibition in aluminium chloride induced dementia of alzheimer’s type in rats. Int. J. Pharmacol. 2018, 14, 879–888. [Google Scholar]
  89. Ferdous, R.; Islam, M.B.; Al-Amin, M.Y. Anticholinesterase and antioxidant activity of Drynaria quercifolia and its ameliorative effect in scopolamine-induced memory impairment in mice. J. Ethnopharmacol. 2024, 319, 117095. [Google Scholar] [CrossRef] [PubMed]
  90. Wang, J.; Wang, F.; Mai, D. Molecular mechanisms of glutamate toxicity in parkinson’s disease. Front. Neurosci. 2020, 14, 585584. [Google Scholar] [CrossRef]
  91. Sultana, R.; Perluigi, M.; Newman, S.F. Redox proteomic analysis of carbonylated brain proteins in mild cognitive impairment and early Alzheimer’s disease. Antioxid. Redox Signal. 2010, 12, 327–336. [Google Scholar] [CrossRef] [PubMed]
  92. Ban, J.; Cho, S.; Jeon, S.; Bae, K.; Song, K.; Seong, Y. 3,4-dihydroxybenzoic acid from Smilacis chinae rhizome protects amyloid beta protein (25–35)-induced neurotoxicity in cultured rat cortical neurons. Neurosci. Lett. 2007, 420, 184–188. [Google Scholar] [CrossRef] [PubMed]
  93. Spiegel, A.M.; Teng, K.M.; Vogt, N.M. Hilar interneuron vulnerability distinguishes aged rats with memory impairment. J. Comp. Neurol. 2013, 521, 3508–3523. [Google Scholar] [CrossRef]
  94. Mert, H.; Kerem, Ö.; Mıs, L.; Yıldırım, S.; Mert, N. Effects of protocatechuic acid against cisplatin-induced neurotoxicity in rat brains: An experimental study. Int. J. Neurosci. 2024, 134, 725–734. [Google Scholar] [CrossRef] [PubMed]
  95. Adefegha, S.A.; Oboh, G.; Omojokun, O.S.; Adefegha, O.M. Alterations of Na+/K+-ATPase, cholinergic and antioxidant enzymes activity by protocatechuic acid in cadmium-induced neurotoxicity and oxidative stress in Wistar rats. Biomed. Pharmacother. 2016, 83, 559–568. [Google Scholar] [CrossRef] [PubMed]
  96. Lv, J.; Xiao, X.; Bi, M. ATP-sensitive potassium channels:a double-edged sword in neurodegenerative diseases. Ageing Res. Rev. 2022, 80, 101676. [Google Scholar] [CrossRef]
  97. Varì, R.; D’Archivio, M.; Filesi, C. Protocatechuic acid induces antioxidant/detoxifying enzyme expression through JNK-mediated Nrf2 activation in murine macrophages. J. Nutr. Biochem. 2011, 22, 409–417. [Google Scholar] [CrossRef]
  98. Muley, M.M.; Thakare, V.N.; Patil, R.R.; Bafna, P.A.; Naik, S.R. Amelioration of cognitive, motor and endogenous defense functions with silymarin, piracetam and protocatechuic acid in the cerebral global ischemic rat model. Life Sci. 2013, 93, 51–57. [Google Scholar] [CrossRef]
  99. Zhang, W.; Liu, Y.; Liao, Y.; Zhu, C.; Zou, Z. GPX4, ferroptosis, and diseases. Biomed. Pharmacother. 2024, 174, 116512. [Google Scholar] [CrossRef]
  100. Sun, Y.; Zheng, Y.; Wang, C. Glutathione depletion induces ferroptosis, autophagy, and premature cell senescence in retinal pigment epithelial cells. Cell Death Dis. 2018, 9, 753. [Google Scholar] [CrossRef]
  101. Hider, R.C.; Kong, X. Glutathione: A key component of the cytoplasmic labile iron pool. Biometals 2011, 24, 1179–1187. [Google Scholar] [CrossRef] [PubMed]
  102. Liu, L.; Vollmer, M.K.; Kelly, M.G. Reactive Gliosis Contributes to Nrf2-Dependent Neuroprotection by Pretreatment with Dimethyl Fumarate or Korean Red Ginseng Against Hypoxic-Ischemia: Focus on Hippocampal Injury. Mol. Neurobiol. 2020, 57, 105–117. [Google Scholar] [CrossRef] [PubMed]
  103. Ji, Y.; Zheng, K.; Li, S. Insight into the potential role of ferroptosis in neurodegenerative diseases. Front. Cell. Neurosci. 2022, 16, 1005182. [Google Scholar] [CrossRef] [PubMed]
Molecules 29 04724 g001
Figure 1. Molecular structure of protocatechuic acid.
Figure 1. Molecular structure of protocatechuic acid.
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Figure 2. Metabolic diagram of protocatechuic acid.
Figure 2. Metabolic diagram of protocatechuic acid.
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Figure 3. Pharmacokinetics of protocatechuic acid.
Figure 3. Pharmacokinetics of protocatechuic acid.
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Figure 4. Ten mechanisms of protocatechuic acid in treating cognitive impairment.
Figure 4. Ten mechanisms of protocatechuic acid in treating cognitive impairment.
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Figure 5. The P38-ERK-JNK and NF-κB pathways.
Figure 5. The P38-ERK-JNK and NF-κB pathways.
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Figure 6. The akt/GSK-3β/MeF2d signaling pathway.
Figure 6. The akt/GSK-3β/MeF2d signaling pathway.
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Table 1. Protocatechuic acid content in different traditional Chinese medicines.
Table 1. Protocatechuic acid content in different traditional Chinese medicines.
NameContent (mg/g)Detection MethodsReferences
rose0.476–1.632Extraction using an ethanol aqueous solution at 180 °C[8]
wolfberry0.1819–0.9114HPLC-MS[9]
schizandra0.0722–0.3569HPLC-DAD[10]
pueraria root0.04HPLC-/MS[11]
scutellaria barbata0.0361–0.066178Supercritical CO2 Extraction[12]
eucommia ulmoides0–0.20694HPLC[13]
acanthopanax senticosus0.003165–0.02312HPLC-DAD[14]
smilax glabra66HSCCC[15]
prunella vulgaris27HPLC-DAD-ESI[16]
solanum nigrum4.1–48LC-MS[17]
hedyotis diffusa0.561UHPLC-MS/MS[18]
plantago seed0.001881–0.004856QAMS[19]
dried tangerine peel0.043–0.075GC-MS, UPLC[20]
euphorbia pekinensis0.02603–0.361LC-MS/MS[21]
cynomorium songaricum0.012134–0.025287LC-MS/MS[22]
rheum officinale0.03899HPLC[23]
salvia miltiorrhiza0.915cce-UV[24]
phellinus igniarius0.016–0.032UHPLC-QqQ[25]
ephedra0.05–0.75HPLC-DAD[26]
sterculia lychnophora0.0219UPLC-MS/MS[27]
cinnamon0.1931–0.2278LC-HRMS[28]
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Liang, S.; Zhao, Z.; Liu, L.; Zhang, Y.; Liu, X. Research Progress on the Mechanisms of Protocatechuic Acid in the Treatment of Cognitive Impairment. Molecules 2024, 29, 4724. https://doi.org/10.3390/molecules29194724

AMA Style

Liang S, Zhao Z, Liu L, Zhang Y, Liu X. Research Progress on the Mechanisms of Protocatechuic Acid in the Treatment of Cognitive Impairment. Molecules. 2024; 29(19):4724. https://doi.org/10.3390/molecules29194724

Chicago/Turabian Style

Liang, Shuzhi, Zhongmin Zhao, Leilei Liu, Yan Zhang, and Xijian Liu. 2024. "Research Progress on the Mechanisms of Protocatechuic Acid in the Treatment of Cognitive Impairment" Molecules 29, no. 19: 4724. https://doi.org/10.3390/molecules29194724

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