Matrix Selection Strategies for MALDI-TOF MS/MS Characterization of Cyclic Tetrapyrroles in Blood and Food Samples
Abstract
:1. Introduction
2. Results and Discussion
2.1. Matrix Evaluation for Protoporphyrin Analysis by MALDI MS
2.2. MALDI MS(/MS) of Phthalocyanines
2.3. MALDI MS(/MS) of Heme b and c in Standards and Real Samples
3. Materials and Methods
3.1. Chemicals
3.2. Instrumentation
3.3. Sample Preparation
3.3.1. Standard Phthalocyanines and Protoporphyrins
3.3.2. Standard Hemoglobin, Myoglobin, and Cytochrome c
3.3.3. Heme in Blood and Food Samples
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Product Ions | Proposed Formula | Loss of Chemical Species | Theoretical m/z Value | MALDI MS/MS | |
---|---|---|---|---|---|
m/z | Relative Intensity% | ||||
CoPP ([C34H32CoN4O4]+) at m/z 619.180 | |||||
B | [C32H29CoN4O4]+• | [CH2CH]• | 592.156 | 592.14 | 5.2 |
C | [C33H31CoN4O2]+• | [HCO2]• | 574.177 | 574.18 | 9.2 |
D | [C32H29CoN4O2]+• | [CH2COOH]• | 560.162 | 560.17 | 100 |
E | [C31H28CoN4O2]+ | [CH2CHCOOH] | 547.154 | 547.12 | 6.4 |
F | [C30H26CoN4]+ | 2×[CH2COOH]• | 501.148 | 501.16 | 32.3 |
G | [C29H23CoN4]+• | [C5H9O4]• | 486.125 | 486.15 | 8.7 |
ZnPP ([C34H32ZnN4O4]+•) at m/z 624.172 | |||||
B | [C33H31ZnN4O2]+ | [HCO2]• | 579.174 | 579.18 | 2.3 |
C | [C32H29ZnN4O2]+ | [CH2COOH]• | 565.158 | 565.16 | 100 |
D | [C31H28ZnN4O2]+ | [CH2CHCOOH] | 552.150 | 552.15 | 14.6 |
E | [C30H26ZnN4]+• | 2×[CH2COOH]• | 506.145 | 506.14 | 20.7 |
F | [C29H23ZnN4]+ | [C5H9O4]• | 491.121 | 491.12 | 7.6 |
G | [C28H21ZnN4]+ | [C6H11O4]• | 477.106 | 477.11 | 3.6 |
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Bianco, M.; Ventura, G.; Calvano, C.D.; Losito, I.; Cataldi, T.R.I.; Monopoli, A. Matrix Selection Strategies for MALDI-TOF MS/MS Characterization of Cyclic Tetrapyrroles in Blood and Food Samples. Molecules 2024, 29, 868. https://doi.org/10.3390/molecules29040868
Bianco M, Ventura G, Calvano CD, Losito I, Cataldi TRI, Monopoli A. Matrix Selection Strategies for MALDI-TOF MS/MS Characterization of Cyclic Tetrapyrroles in Blood and Food Samples. Molecules. 2024; 29(4):868. https://doi.org/10.3390/molecules29040868
Chicago/Turabian StyleBianco, Mariachiara, Giovanni Ventura, Cosima Damiana Calvano, Ilario Losito, Tommaso R. I. Cataldi, and Antonio Monopoli. 2024. "Matrix Selection Strategies for MALDI-TOF MS/MS Characterization of Cyclic Tetrapyrroles in Blood and Food Samples" Molecules 29, no. 4: 868. https://doi.org/10.3390/molecules29040868