Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes
Abstract
:1. Introduction
2. Experimental Section
2.1. Chemicals and animals
2.2. Genotoxicity/antigenotoxicity protocol
2.2.1. Experimental design
2.3. Unicellular alkaline electrophoresis (comet) assay
2.4. Crystallization, melting point determination, and spectroscopic analysis
3. Results and Discussion
4. Conclusions
References
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Agent/dose | Time (h) | Grades of damage (%) | |||
---|---|---|---|---|---|
G0 | G1 | G2 | G3 | ||
Corn oil
0.02 mL | 4 | 84 | 13 | 2 | 1 |
10 | 87 | 11 | 2 | 0 | |
16 | 92 | 4 | 3 | 1 | |
DMSO
0.02 mL | 4 | 77 | 17 | 4 | 2 |
10 | 87 | 5 | 4 | 4 | |
16 | 86 | 8 | 4 | 2 | |
Man
700 mg/kg | 4 | 91 | 7 | 2 | 0 |
10 | 85 | 10 | 3 | 2 | |
16 | 89 | 6 | 3 | 2 | |
AFB1 1.0 mg/kg | 4 | 72 | 12 | 8 | 8 |
10 | 2 | 9 | 24 | 65 | |
16 | 18 | 18 | 27 | 37 | |
Man + AFB1 100 + 1.0 mg/kg | 4 | 67 | 8 | 10 | 15 |
10 | 4 | 10 | 29 | 57 | |
16 | 16 | 20 | 30 | 34 | |
Man + AFB1 400 + 1.0 mg/kg | 4 | 75 | 12 | 8 | 5 |
10 | 23 | 20 | 23 | 35 | |
16 | 32 | 15 | 25 | 28 | |
Man + AFB1 700 + 1.0 mg/kg | 4 | 80 | 14 | 3 | 3 |
10 | 42 | 18 | 17 | 23 | |
16 | 36 | 28 | 19 | 17 |
Chemical | Melting point (°C) |
---|---|
AFB1/Man | 182.5 |
AFB1 | 132.5 |
Man | 246.0 |
© 2009 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license ( http://creativecommons.org/licenses/by/3.0/).
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Madrigal-Santillán, E.; Morales-González, J.A.; Sánchez-Gutiérrez, M.; Reyes-Arellano, A.; Madrigal-Bujaidar, E. Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes. Int. J. Mol. Sci. 2009, 10, 395-406. https://doi.org/10.3390/ijms10020395
Madrigal-Santillán E, Morales-González JA, Sánchez-Gutiérrez M, Reyes-Arellano A, Madrigal-Bujaidar E. Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes. International Journal of Molecular Sciences. 2009; 10(2):395-406. https://doi.org/10.3390/ijms10020395
Chicago/Turabian StyleMadrigal-Santillán, Eduardo, José Antonio Morales-González, Manuel Sánchez-Gutiérrez, Alicia Reyes-Arellano, and Eduardo Madrigal-Bujaidar. 2009. "Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes" International Journal of Molecular Sciences 10, no. 2: 395-406. https://doi.org/10.3390/ijms10020395