Microsatellite Analysis in Multistage Carcinogenesis of Esophageal Squamous Cell Carcinoma from Chongqing in Southern China
Abstract
:1. Introduction
2. Results
3. Discussion
4. Experimental Section
4.1. Sample Collection
4.2. Tissue Cell Acquisition by Using Microdissection
4.3. Extraction of Genomic DNA
4.4. Loss of Heterozygosity (LOH) Analysis
4.5. Statistical Analysis
5. Conclusions
Acknowledgements
- Conflict of Interest The authors declare no conflict of interest.
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Marker | Location | LGD a sample (%) | HGD a sample (%) | SCC a sample (%) |
---|---|---|---|---|
3S1597 | 3p25 | 4/21 (19) | 12/22 (54.5) | 18/22 (81.8) |
D3S2452 | 3p21-p14 | 7/25 (28) | 20/25 (80) | 18/25 (72) |
D3S1285 | 3p14 | 3/19 (15.8) | 14/21 (66.7) | 16/21 (76.2) |
D4S174 | 4p14-p13 | 2/27 (7.4) | 7/27 (25.9) | 12/27 (44.4) |
D5S409 | 5q14-q15 | 0/16 (0) | 7/16 (43.7) | 8/16 (50) |
D5S2501 | 5q21-q23.3 | 1/13 (7.7) | 7/13 (53.8) | 7/13 (53.8) |
D8S261 | 8p22-p21.3 | 0/18 (0) | 5/18 (27.8) | 6/18 (33.3) |
D9S157 | 9p23-p22 | 0/23 (0) | 16/23 (69.5) | 15/23 (65.2) |
D9S111 | 9q12-q21.1 | 0/25 (0) | 9/25 (36) | 15/25 (60) |
D9S125 | 9q34-q34 | 9/27 (33.3) | 21/27 (77.8) | 23/27 (85.2) |
D11S1338 | 11p15.5 | 0/29 (0) | 10/29 (34.5) | 11/29 (37.9) |
D13S175 | 13q13 | 0/22 (0) | 10/23 (43.5) | 12/23 (52.2) |
D13S153 | 13q14.2 | 4/24 (16.7) | 10/24 (41.7) | 13/24 (54.2) |
D13S173 | 13q32-q34 | 0/24 (0) | 5/24 (20.8) | 11/24 (45.8) |
D17S786 | 17p13.1 | 5/23 (21.7) | 13/23 (56.5) | 16/23 (69.5) |
D17S261 | 17p11.2 | 0/22 (0) | 10/22 (45.4) | 11/22 (50) |
Total b | 35/358 (9.8) | 176/362 (48.6) | 212/362 (58.5) |
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Liu, M.; Zhang, F.; Liu, S.; Zhao, W.; Zhu, J.; Zhang, X. Microsatellite Analysis in Multistage Carcinogenesis of Esophageal Squamous Cell Carcinoma from Chongqing in Southern China. Int. J. Mol. Sci. 2011, 12, 7401-7409. https://doi.org/10.3390/ijms12117401
Liu M, Zhang F, Liu S, Zhao W, Zhu J, Zhang X. Microsatellite Analysis in Multistage Carcinogenesis of Esophageal Squamous Cell Carcinoma from Chongqing in Southern China. International Journal of Molecular Sciences. 2011; 12(11):7401-7409. https://doi.org/10.3390/ijms12117401
Chicago/Turabian StyleLiu, Ming, Feng Zhang, Shen Liu, Wen Zhao, Jing Zhu, and Xiaoli Zhang. 2011. "Microsatellite Analysis in Multistage Carcinogenesis of Esophageal Squamous Cell Carcinoma from Chongqing in Southern China" International Journal of Molecular Sciences 12, no. 11: 7401-7409. https://doi.org/10.3390/ijms12117401