Next Article in Journal
Eclipsed Acetaldehyde as a Precursor for Producing Vinyl Alcohol
Next Article in Special Issue
Drug Conjugates Such as Antibody Drug Conjugates (ADCs), Immunotoxins and Immunoliposomes Challenge Daily Clinical Practice
Previous Article in Journal
Primary, Secondary Metabolites, Photosynthetic Capacity and Antioxidant Activity of the Malaysian Herb Kacip Fatimah (Labisia Pumila Benth) Exposed to Potassium Fertilization under Greenhouse Conditions
Previous Article in Special Issue
Genomic Polymorphism of Human Papillomavirus Type 52 in Women from Northeast China
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells

Chair and Department of Microbiology and Immunology, Medical University of Silesia in Katowice, Jordana 19, 41-808 Zabrze, Poland
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2012, 13(11), 15343-15359; https://doi.org/10.3390/ijms131115343
Submission received: 24 October 2012 / Revised: 13 November 2012 / Accepted: 14 November 2012 / Published: 20 November 2012
(This article belongs to the Special Issue Advances in Molecular Oncology (special issue))

Abstract

Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in cancer cells without toxicity to normal cells. TRAIL binds to death receptors, TRAIL-R1 (DR4) and TRAIL-R2 (DR5) expressed on cancer cell surface and activates apoptotic pathways. Endogenous TRAIL plays an important role in immune surveillance and defense against cancer cells. However, as more tumor cells are reported to be resistant to TRAIL mediated death, it is important to search for and develop new strategies to overcome this resistance. Chalcones can sensitize cancer cells to TRAIL-induced apoptosis. We examined the cytotoxic and apoptotic effects of TRAIL in combination with four chalcones: chalcone, isobavachalcone, licochalcone A and xanthohumol on HeLa cancer cells. The cytotoxicity was measured by MTT and LDH assays. The apoptosis was detected using annexin V-FITC staining by flow cytometry and fluorescence microscopy. Death receptor expression was analyzed using flow cytometry. The decreased expression of death receptors in cancer cells may be the cause of TRAIL-resistance. Chalcones enhance TRAIL-induced apoptosis in HeLa cells through increased expression of TRAIL-R2. Our study has indicated that chalcones augment the antitumor activity of TRAIL and confirm their cancer chemopreventive properties.
Keywords: TRAIL; chalcones; apoptosis; death receptors; cancer cells; chemoprevention TRAIL; chalcones; apoptosis; death receptors; cancer cells; chemoprevention

Share and Cite

MDPI and ACS Style

Szliszka, E.; Jaworska, D.; Ksek, M.; Czuba, Z.P.; Król, W. Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells. Int. J. Mol. Sci. 2012, 13, 15343-15359. https://doi.org/10.3390/ijms131115343

AMA Style

Szliszka E, Jaworska D, Ksek M, Czuba ZP, Król W. Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells. International Journal of Molecular Sciences. 2012; 13(11):15343-15359. https://doi.org/10.3390/ijms131115343

Chicago/Turabian Style

Szliszka, Ewelina, Dagmara Jaworska, Małgorzata Ksek, Zenon P. Czuba, and Wojciech Król. 2012. "Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells" International Journal of Molecular Sciences 13, no. 11: 15343-15359. https://doi.org/10.3390/ijms131115343

APA Style

Szliszka, E., Jaworska, D., Ksek, M., Czuba, Z. P., & Król, W. (2012). Targeting Death Receptor TRAIL-R2 by Chalcones for TRAIL-Induced Apoptosis in Cancer Cells. International Journal of Molecular Sciences, 13(11), 15343-15359. https://doi.org/10.3390/ijms131115343

Article Metrics

Back to TopTop