The Apoptotic Volume Decrease Is an Upstream Event of MAP Kinase Activation during Staurosporine-Induced Apoptosis in HeLa Cells
Abstract
:1. Introduction
2. Results
2.1. VSOR Blocker Sensitivity of STS-Induced AVD and Phosphorylation of p38 and JNK
2.2. ASK1 Dependence of STS-Induced Caspase Activation but not of AVD
2.3. Induction of Phosphorylation of p38 and JNK by Sustained Cell Shrinkage
3. Discussion
4. Experimental Section
4.1. Chemicals and Antibodies
4.2. Cell Culture and Apoptosis Induction
4.3. Cell Viability Measurements
4.4. Caspase-3/7 Activity Measurements
4.5. Cell Volume Measurements
4.6. Western Blotting
4.7. RNA Interference
4.8. Quantitative RT-PCR
4.9. Statistical Analysis
5. Conclusions
Acknowledgments
References
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Hasegawa, Y.; Shimizu, T.; Takahashi, N.; Okada, Y. The Apoptotic Volume Decrease Is an Upstream Event of MAP Kinase Activation during Staurosporine-Induced Apoptosis in HeLa Cells. Int. J. Mol. Sci. 2012, 13, 9363-9379. https://doi.org/10.3390/ijms13079363
Hasegawa Y, Shimizu T, Takahashi N, Okada Y. The Apoptotic Volume Decrease Is an Upstream Event of MAP Kinase Activation during Staurosporine-Induced Apoptosis in HeLa Cells. International Journal of Molecular Sciences. 2012; 13(7):9363-9379. https://doi.org/10.3390/ijms13079363
Chicago/Turabian StyleHasegawa, Yuichi, Takahiro Shimizu, Nobuyuki Takahashi, and Yasunobu Okada. 2012. "The Apoptotic Volume Decrease Is an Upstream Event of MAP Kinase Activation during Staurosporine-Induced Apoptosis in HeLa Cells" International Journal of Molecular Sciences 13, no. 7: 9363-9379. https://doi.org/10.3390/ijms13079363