Alteration of Tight Junction Gene Expression by Calcium- and Vitamin D-Deficient Diet in the Duodenum of Calbindin-Null Mice
Abstract
:1. Introduction
2. Results
2.1. Regulation of Serum Calcium Concentrations after the Consumption of Calcium-Deficient and Calcium/Vitamin D-Deficient Diets
2.2. Expression of Tight Junction Genes in the Duodenum
2.3. Regulation of Duodenal Tight Junction Protein Expression
2.4. Localization of Duodenal Tight Junction Proteins
3. Experimental Section
3.1. Animals
3.2. Experimental Treatments
3.3. Serum Calcium Concentration Analysis
3.4. Quantitative Real-Time PCR
3.5. Western Blot Analysis
3.6. Immunohistochemistry
3.7. Data Analysis
4. Conclusions
Diet | WT | CaBP-9K KO | CaBP-28K KO | DKO |
---|---|---|---|---|
Normal | 9.6 ± 0.15 | 9.8 ± 0.12 | 9.2 ± 0.10 | 9.6 ± 0.10 |
Ca2+ def | 9.4 ± 0.06 * | 9.4 ± 0.06 * | 9.2 ± 0.06 | 9.0 ± 0.10 * |
Ca2+/VD def | 8.3 ± 0.15 ** | 9.4 ± 0.12 * | 8.2 ± 0.06 ** | 7.5 ± 0.06 ** |
Diet | WT | CaBP-9K KO | CaBP-28K KO | DKO |
---|---|---|---|---|
Normal | - | OCLN↑, CLDN2↑, CLDN15↑ | CLDN10b↑ | CLDN10b↑ |
Ca2+ def | - | CLDN10b↑ | - | - |
Ca2+/VD def | - | OCLN↓, ZO-1↓, CLDN12↑ CLDN15↓ | CLDN15↑ | - |
Gene | Forward | Reverse |
---|---|---|
β-actin | 5′-ACAGGCATTGTGATGGACTC-3′ | 5′-ATTTCCCTCTCAGCTGTGGT-3′ |
OCLN | 5′-ACTGGGTCAGGGAATATCCA-3′ | 5′-TCAGCAGCAGCCATGTACTC-3′ |
ZO-1 | 5′-ACTCCCACTTCCCCAAAAAC-3′ | 5′-CCACAGCTGAAGGACTCACA-3′ |
CLDN2 | 5′-TGGTTCCTGACAGCATGAAA-3′ | 5′-CTTTGGGCTGTTGAGCAGAT-3′ |
CLDN10b | 5′-TCGCCTTCGTAGTCTCCATC-3′ | 5′-TCTCCTTCTCCGCCTTGATAC-3′ |
CLDN12 | 5′-AGGAAGTTTGAGCCGGTCTT-3′ | 5′-CGTGATGAATAGGGCTGTGA-3′ |
CLDN15 | 5′-GCCTCTTTCTAGGCATGGTG-3′ | 5′-TCCAGCATACAGTGGGTTGA-3′ |
Acknowledgments
Conflicts of Interest
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Hwang, I.; Yang, H.; Kang, H.-S.; Ahn, C.; Hong, E.-J.; An, B.-S.; Jeung, E.-B. Alteration of Tight Junction Gene Expression by Calcium- and Vitamin D-Deficient Diet in the Duodenum of Calbindin-Null Mice. Int. J. Mol. Sci. 2013, 14, 22997-23010. https://doi.org/10.3390/ijms141122997
Hwang I, Yang H, Kang H-S, Ahn C, Hong E-J, An B-S, Jeung E-B. Alteration of Tight Junction Gene Expression by Calcium- and Vitamin D-Deficient Diet in the Duodenum of Calbindin-Null Mice. International Journal of Molecular Sciences. 2013; 14(11):22997-23010. https://doi.org/10.3390/ijms141122997
Chicago/Turabian StyleHwang, Inho, Hyun Yang, Hong-Seok Kang, Changhwan Ahn, Eui-Ju Hong, Beum-Soo An, and Eui-Bae Jeung. 2013. "Alteration of Tight Junction Gene Expression by Calcium- and Vitamin D-Deficient Diet in the Duodenum of Calbindin-Null Mice" International Journal of Molecular Sciences 14, no. 11: 22997-23010. https://doi.org/10.3390/ijms141122997