Aerobic Interval Training Attenuates Mitochondrial Dysfunction in Rats Post-Myocardial Infarction: Roles of Mitochondrial Network Dynamics
Abstract
:1. Introduction
2. Results
2.1. Effects of MI and AIT on Left Ventricular Tissue Microstructure
2.2. Effects of MI and AIT on Membrane Potential and Cytochrome C Leakage
2.3. Effects of MI and AIT on Mitochondrial Respiratory Functions, Electron Transport Chain (ETC) Complex Activities and Protein Expression of Citrate Synthase (CS)
2.4. Effects of MI and AIT on Remodeling of Mitochondrial Dynamics and Oxidative Signaling
2.5. Effects of MI and AIT on PGC-1α and Tfam
3. Discussion
4. Experimental Section
4.1. Animals
4.2. In Vivo Myocardial Infarction Model
4.3. Experimental Grouping and Animal Use
4.4. Treadmill AIT Protocol
4.5. HE Staining
4.6. Myocardial Mitochondria Preparations
4.7. Mitochondrial Enzyme Activity Assay
4.8. Mitochondrial Respiratory Activity Determination
4.9. Measuring Mitochondrial Membrane Potential
4.10. Nuclear Protein Extraction
4.11. Western Blot Analysis
4.12. Statistical Analysis
5. Conclusions
Acknowledgments
Conflicts of Interest
References
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Jiang, H.-K.; Wang, Y.-H.; Sun, L.; He, X.; Zhao, M.; Feng, Z.-H.; Yu, X.-J.; Zang, W.-J. Aerobic Interval Training Attenuates Mitochondrial Dysfunction in Rats Post-Myocardial Infarction: Roles of Mitochondrial Network Dynamics. Int. J. Mol. Sci. 2014, 15, 5304-5322. https://doi.org/10.3390/ijms15045304
Jiang H-K, Wang Y-H, Sun L, He X, Zhao M, Feng Z-H, Yu X-J, Zang W-J. Aerobic Interval Training Attenuates Mitochondrial Dysfunction in Rats Post-Myocardial Infarction: Roles of Mitochondrial Network Dynamics. International Journal of Molecular Sciences. 2014; 15(4):5304-5322. https://doi.org/10.3390/ijms15045304
Chicago/Turabian StyleJiang, Hong-Ke, You-Hua Wang, Lei Sun, Xi He, Mei Zhao, Zhi-Hui Feng, Xiao-Jiang Yu, and Wei-Jin Zang. 2014. "Aerobic Interval Training Attenuates Mitochondrial Dysfunction in Rats Post-Myocardial Infarction: Roles of Mitochondrial Network Dynamics" International Journal of Molecular Sciences 15, no. 4: 5304-5322. https://doi.org/10.3390/ijms15045304