Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44
Abstract
:1. Introduction
2. Results
2.1. Identification of RCAR1/PYL9-Interacting Proteins by Yeast Two-Hybrid Screening
2.2. Demonstration of the Physical Interaction between RCAR1/PYL9 and AtMYB44 in Vitro and in Vivo
2.3. Analyses of the Interactions between RCARs/PYR1/PYLs and Other Members of the 22nd Subgroup of R2R3-MYBs
2.4. AtMYB44 Negatively Regulates the Expression of ABA-Responsive Gene RAB18
2.5. AtMYB44 and ABI1 Compete for Binding to RCAR1/PYL9
2.6. AtMYB44 Probably Reduces the Inhibitory Effect of RCAR1/PYL9 on ABI1 Activity in Vitro and in Vivo
3. Discussion
4. Experimental Section
4.1. Plant Materials and Chemicals
4.2. Plasmid Construction
4.3. Yeast Two-Hybrid Assay
4.4. GST Pull-Down Assay
4.5. BiFC Assay
4.6. RNA Isolation and qRT-PCR Analysis
4.7. Protoplast Isolation and Transient Activation Assay
4.8. PP2C Enzyme Assay
5. Conclusions
Supplementary Information
ijms-15-08473-s001.pdfAcknowledgments
Conflicts of Interest
References
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Clone | Accession Numbers in TAIR a | AA Sequence in Library | AA Sequence in TAIR a | Numbers of Clones |
---|---|---|---|---|
AtMYB44 | AT5G67300 | 48–305 | 305 | 3 |
AtMYB70 | AT2G23290 | 68–144 | 309 | 1 |
AtMYB77 | AT3G50060 | 26–286 | 301 | 1 |
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Li, D.; Li, Y.; Zhang, L.; Wang, X.; Zhao, Z.; Tao, Z.; Wang, J.; Wang, J.; Lin, M.; Li, X.; et al. Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44. Int. J. Mol. Sci. 2014, 15, 8473-8490. https://doi.org/10.3390/ijms15058473
Li D, Li Y, Zhang L, Wang X, Zhao Z, Tao Z, Wang J, Wang J, Lin M, Li X, et al. Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44. International Journal of Molecular Sciences. 2014; 15(5):8473-8490. https://doi.org/10.3390/ijms15058473
Chicago/Turabian StyleLi, Dekuan, Ying Li, Liang Zhang, Xiaoyu Wang, Zhe Zhao, Zhiwen Tao, Jianmei Wang, Jin Wang, Min Lin, Xufeng Li, and et al. 2014. "Arabidopsis ABA Receptor RCAR1/PYL9 Interacts with an R2R3-Type MYB Transcription Factor, AtMYB44" International Journal of Molecular Sciences 15, no. 5: 8473-8490. https://doi.org/10.3390/ijms15058473