Elucidation of the Specific Formation of Homo- and Heterodimeric Forms of ThbZIP1 and Its Role in Stress
Abstract
:1. Introduction
2. Results and Discussion
2.1. Analysis of the Hetero- and Homodimers of ThbZIP1
2.2. The Binding of ThbZIP2, ThbZIP4, and ThbZIP7 to C-Box Motifs
2.3. The Expression of ThbZIPs in T. hispida Can Be Induced by Salt, ABA (Abscisic Acid), and Drought
2.4. Targeting ThbZIPs to the Nucleus
3. Experimental Section
3.1. Plant Materials and Growth Conditions
3.2. Cloning the ThbZIP Gene Sequences
3.3. Yeast Transactivation Activity Analysis
3.4. Autoactivation, Heterodimer, and Homodimer Assays for ThbZIP1
3.5. Transient Expression Assays
3.6. Real-Time PCR Analysis of Gene Expression
3.7. The Subcellular Localization of the ThbZIP Proteins
3.8. Statistical Analysis
4. Conclusions
Supplementary Files
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Nie, X.; Ji, X.; Liu, Y.; Zheng, L.; Wang, Y. Elucidation of the Specific Formation of Homo- and Heterodimeric Forms of ThbZIP1 and Its Role in Stress. Int. J. Mol. Sci. 2014, 15, 10005-10017. https://doi.org/10.3390/ijms150610005
Nie X, Ji X, Liu Y, Zheng L, Wang Y. Elucidation of the Specific Formation of Homo- and Heterodimeric Forms of ThbZIP1 and Its Role in Stress. International Journal of Molecular Sciences. 2014; 15(6):10005-10017. https://doi.org/10.3390/ijms150610005
Chicago/Turabian StyleNie, Xianguang, Xiaoyu Ji, Yujia Liu, Lei Zheng, and Yucheng Wang. 2014. "Elucidation of the Specific Formation of Homo- and Heterodimeric Forms of ThbZIP1 and Its Role in Stress" International Journal of Molecular Sciences 15, no. 6: 10005-10017. https://doi.org/10.3390/ijms150610005